FastQCFastQC Report
Fri 10 Feb 2017
SRR2568707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53855
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT37817.0207037415281786No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT12762.369325039457803TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9091.6878655649429024No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT7181.3332095441463188RNA PCR Primer, Index 1 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6651.23479714046978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.7724445269705691No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3390.6294680159688051No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2940.545910314734008No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT2580.4790641537461703TruSeq Adapter, Index 1 (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2230.41407483056355027No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG1840.34165815616005946TruSeq Adapter, Index 1 (96% over 28bp)
GTACATGGGATGTGGACACCTGAATATGCAAGGTGTCTTCCTGAAAAGCC1830.3398013183548417No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1620.3008077244452697No Hit
CATTAAATCAAATGGAGGGAGGGTGTGAAGGAAAAGGGAGCTGAGACCAG1530.2840961841983103No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1520.28223934639309256No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG1510.28038250858787483TruSeq Adapter, Index 1 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.26924148175656853No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC1440.26738464395135086No Hit
TGTAAGAGGAGATCAGCTGCTCTTCACCACAATTACTACCAAATACTAGG1400.25995729273047996No Hit
GTGAGAGGCTAATCCTTTCCATCTCAGGGCTATCACATTCACACACACAT1390.25810045492526223No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT1380.25624361712004456No Hit
CTTTTATGTTGCAAATGTAATAAACTGACATGCCTTTGACTTTCACACAC1350.25067310370439144No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1320.2451025902887383No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.23953207687308514No Hit
GTACATGGGGATCAAAGACGCAGATCAGGCAGGACTTCCAGTGGAAGCAA1250.23210472565221427No Hit
GTACAATGGGGGGGTGTTACCAAGGCTGCAGAGAAGCAGATGAAGGACAA1230.22839105004177884No Hit
GTACATGGGGGGACAAGCGAATAAGTGCACATGGTGGATGCCTTGGCGAT1200.2228205366261257No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.21725002321047257No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1170.21725002321047257No Hit
GTACATGGGAGAAGTCTGTGGCTTTGTCAGATATGTCACCCAAGTAAATG1110.20610899637916627No Hit
ACATTGGGGTCTGTCAGAACAAGACATCTGCTCATATGAAGCCAGACTCA1080.20053848296351312No Hit
GTAATAAACTGACATGCCTTTGACTTTCACACACTCACAACAAGATCATT1000.18568378052177142No Hit
CCCTAAAAGGGTGGGTTGCCCCATTCGGAAATCTGCGGATCAAAGTGTGT970.18011326710611827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA960.17825642930090058No Hit
TTACATAACTCTGCAGTGTTTCTCAAGTGAGCTTTTCATTTTAATCAGAT950.17639959149568285No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC930.17268591588524743No Hit
GAGTACAATGGGGGGGTGTTACCAAGGCTGCAGAGAAGCAGATGAAGGAC920.17082907808002973No Hit
CCCCAATGTCATGCCTACAGTCCAAACACACACCCAGCCTCTGGGAAAAC910.16897224027481197No Hit
GTGTTTGGACTGTAGGCATGACATTGGGGTCTGTCAGAACAAGACATCTG910.16897224027481197No Hit
GGCTAATCCTTTCCATCTCAGGGCTATCACATTCACACACACATTAAATC900.16711540246959428No Hit
TCTCTGTACAATGTCGATTATAATTATGCAAACAACAAAACTGTCAGTCT890.16525856466437658No Hit
CATTTCAAGTGGTTGGCCATGTAAGAGGAGATCAGCTGCTCTTCACCACA890.16525856466437658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.15783121344350573No Hit
CCTGAATATGCAAGGTGTCTTCCTGAAAAGCCCACACATTTCAAGTGGTT850.15783121344350573No Hit
GTGTAAGCTTTTAAAGTTGTAGGTTAAACCTCCTGTTGTTATCTTTGCTG820.15226070002785258No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC820.15226070002785258No Hit
GTACATGGGCCACTAAAAATACAAAAATTAGCTGGGTGTGGTGGCGTGCA810.15040386222263485No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA790.14669018661219943No Hit
GTACAATGTCGATTATAATTATGCAAACAACAAAACTGTCAGTCTGTTAG790.14669018661219943No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA780.14483334880698173No Hit
GTCCAAACACACACCCAGCCTCTGGGAAAACACACCCTGCAAGCTTCTGG780.14483334880698173No Hit
GTTTTACAACATCAGCCCATGATTTTGCAACAATCAAGTTGGCTTCAGAA780.14483334880698173No Hit
GTACTTTTATGTTGCAAATGTAATAAACTGACATGCCTTTGACTTTCACA750.13926283539132858No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC740.13740599758611086Illumina PCR Primer Index 5 (95% over 24bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT730.13554915978089313No Hit
ACCAAATACTAGGACTAGTTCCTGCTGTTGCTCTCGAAAGTGCCATATTC710.1318354841704577No Hit
TCTTTATCCTCAGCTTCTTCTTCCTCTTTACGCTTCTTGTCCTCTTCTTC710.1318354841704577No Hit
GTACATGGGGGGGGGATGGAAAGAAGCCAACCAGCTGCTGACAAGCCAGA710.1318354841704577No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT690.12812180856002228No Hit
GTCTGAGGGTGTGCAGGTTCCCTCTGTGCCCATCCAGCAGTTCCCCACGG690.12812180856002228No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT670.12440813294958684No Hit
GTAGAATGCCATGCTGACTCCGGCTGATGGATGCTCTCTTCGCAGGCAGG650.12069445733915143No Hit
CCTTAAGTCACTGAATTAAGGGTTAAAAAAAGTTTAAAGAGGTGGCTACC650.12069445733915143No Hit
GAATTAAGGGTTAAAAAAAGTTTAAAGAGGTGGCTACCACATTCCAGATA640.11883761953393371No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.116980781728716No Hit
GAGTACATGGGGGGACAAGCGAATAAGTGCACATGGTGGATGCCTTGGCG630.116980781728716No Hit
CCCTCAGACCAGTCGGCCACCTCAGGCTGAGCAGCAGTGAACTCAGGAGC630.116980781728716No Hit
TCTCAGGGCTATCACATTCACACACACATTAAATCAAATGGAGGGAGGGT620.11512394392349828No Hit
GACTAGTTCCTGCTGTTGCTCTCGAAAGTGCCATATTCCTGAGTCTGGCT620.11512394392349828No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA620.11512394392349828No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG610.11326710611828056No Hit
GACTAAAACTTCCAGTACAACGTTGAATAGAAGTGAGAGTAGATTCCCTG610.11326710611828056No Hit
AGTAAATGCTTTGTAGATCTGATTAAAATGAAAAGCTCACTTGAGAAACA600.11141026831306285No Hit
ATATAGGGACATATCTGCTAAAAGAACCCATGAATTTTTCAGATAGTGTC600.11141026831306285No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.10955343050784515No Hit
GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATA580.10769659270262744No Hit
GTACATGGGGATGTGGTGGATGCTGGCCAGGGAAGTACTCCGCATGCGAG580.10769659270262744No Hit
CATTAGGATAGGGTAGGCTAAGTTCCCTATGCTGCTAGCATCCTAAGATG570.10583975489740971No Hit
TTTTTATTCTTTGTGTACAAAGACTAGCATGATCGGTTCCACTTGGTAGT570.10583975489740971No Hit
GACCAGAAGCTTGCAGGGTGTGTTTTCCCAGAGGCTGGGTGTGTGTTTGG570.10583975489740971No Hit
TACTAGGACTAGTTCCTGCTGTTGCTCTCGAAAGTGCCATATTCCTGAGT570.10583975489740971No Hit
CAATATAGGGACATATCTGCTAAAAGAACCCATGAATTTTTCAGATAGTG560.103982917092192No Hit
CCTATGTATTCAATATGCAATACCCTAAAAGGGTGGGTTGCCCCATTCGG560.103982917092192No Hit
GTATCAACGCAGAGTACATGGGGATCAAAGACGCAGATCAGGCAGGACTT550.10212607928697429No Hit
GCTCAGGACCACTCAGTGGTGGCTCCAACCCACTCAGTGGCCTGCGCTGT550.10212607928697429No Hit
GTGTATCTCCAAACCTTAAGTCACTGAATTAAGGGTTAAAAAAAGTTTAA540.10026924148175656No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA540.10026924148175656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCTA156.5836805E-494.999075
TGCCCTG200.00206340571.2493065
GCTAATC200.00206340571.2493068
CTAATCC200.00206340571.2493069
GTACCCA250.00497722657.0524181
ACTTGAC250.004995585456.9994472
CCTGACT250.004995585456.9994478
ATTGCCC250.004995585456.9994473
GGCTAAT250.004995585456.9994477
CTTGACT250.004995585456.9994473
GACTTTA250.004995585456.9994476
AATTGCC250.004995585456.9994472
TTGCCCT250.004995585456.9994474
TGAGAGG250.004995585456.9994472
TATGTTG405.342538E-447.4995355
TGGGATG601.4658417E-647.4995356
ACAATGG405.342538E-447.4995353
ATAAGTG459.543903E-442.221815
TAAGTGC459.543903E-442.221816
TTATGTT459.543903E-442.221814
Produced by FastQC (version 0.11.5)