##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568707_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53855 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39465230712097 34.0 31.0 34.0 30.0 34.0 2 32.44213164980039 34.0 31.0 34.0 31.0 34.0 3 32.49040943273605 34.0 31.0 34.0 30.0 34.0 4 36.00092841890261 37.0 35.0 37.0 35.0 37.0 5 36.07795005106304 37.0 35.0 37.0 35.0 37.0 6 36.1415653142698 37.0 36.0 37.0 35.0 37.0 7 36.07642744406276 37.0 35.0 37.0 35.0 37.0 8 36.07085693064711 37.0 36.0 37.0 35.0 37.0 9 37.873437935196364 39.0 38.0 39.0 35.0 39.0 10-11 37.75235354191811 39.0 38.0 39.0 35.0 39.0 12-13 37.67108903537276 39.0 37.5 39.0 35.0 39.0 14-15 38.81425123015505 40.0 38.0 41.0 34.5 41.0 16-17 38.75381115959521 40.0 38.0 41.0 34.5 41.0 18-19 38.94085043171479 40.5 38.5 41.0 35.0 41.0 20-21 39.01981245938167 41.0 39.0 41.0 35.0 41.0 22-23 38.946708754990254 41.0 38.0 41.0 35.0 41.0 24-25 38.75290130907065 40.5 38.0 41.0 35.0 41.0 26-27 38.527406926005014 40.0 38.0 41.0 34.0 41.0 28-29 38.34398848760561 40.0 37.5 41.0 34.0 41.0 30-31 38.07230526413518 40.0 37.0 41.0 34.0 41.0 32-33 37.56741249651843 40.0 35.0 41.0 33.0 41.0 34-35 37.240098412403675 40.0 35.0 41.0 33.0 41.0 36-37 36.94039550645252 40.0 35.0 41.0 31.5 41.0 38-39 36.29118930461424 39.0 35.0 41.0 30.0 41.0 40-41 36.19455946523071 39.0 35.0 41.0 30.0 41.0 42-43 35.885628075387615 39.0 35.0 41.0 28.0 41.0 44-45 35.48067031844768 39.0 35.0 41.0 25.0 41.0 46-47 34.83125986445084 39.0 35.0 41.0 21.5 41.0 48-49 34.23480642465881 38.5 34.0 41.0 13.5 41.0 50-51 33.584736793241106 38.5 33.0 41.0 10.0 41.0 52-53 33.71529105932596 39.0 34.0 41.0 10.0 41.0 54-55 33.40664747934268 39.0 33.0 41.0 8.0 41.0 56-57 32.971339708476464 38.0 33.0 40.0 8.0 41.0 58-59 32.643682109367745 38.0 32.0 40.0 7.5 41.0 60-61 31.951137313155698 37.0 30.5 40.0 7.0 41.0 62-63 31.322653421223656 36.5 28.5 40.0 7.0 41.0 64-65 30.625392256986352 36.0 26.0 39.0 7.0 41.0 66-67 29.622096369882094 35.0 22.0 39.0 7.0 41.0 68-69 28.416767245381116 35.0 16.5 38.5 7.0 41.0 70-71 27.651322996936216 35.0 13.5 37.0 7.0 40.5 72-73 27.10690743663541 35.0 10.0 37.0 7.0 40.0 74-75 26.821892117723515 35.0 10.0 36.5 7.0 39.0 76-77 25.56729180206109 32.0 10.0 35.0 7.0 37.5 78-79 26.36294680159688 34.0 10.0 35.0 7.0 38.5 80-81 26.287094977253737 34.0 10.0 35.0 7.0 37.0 82-83 26.12620926562065 34.0 10.0 35.0 7.0 37.0 84-85 25.906498932318264 34.0 10.0 35.0 7.0 36.5 86-87 25.774774858416116 34.0 10.0 35.0 7.0 36.0 88-89 25.646838733636617 34.0 10.0 35.0 7.0 36.0 90-91 25.54237303871507 34.0 10.0 35.0 7.0 35.5 92-93 25.414752576362453 34.0 10.0 35.0 7.0 35.0 94-95 25.356735679138428 34.0 10.0 35.0 7.0 35.0 96-97 25.293259678767058 34.0 10.0 35.0 7.0 35.0 98-99 25.242308049391887 34.0 10.0 35.0 7.0 35.0 100-101 24.38336273326525 31.0 10.0 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27875035836159867 1101 2 -0.5132972446451447 1101 3 -0.3003244603087225 1101 4 0.13247405558820446 1101 5 -0.02626599295165022 1101 6 0.17320721546335704 1101 7 0.1959956566907053 1101 8 0.07912840763825102 1101 9 0.3468108923215283 1101 10-11 0.200007450913958 1101 12-13 -0.033848672498479004 1101 14-15 0.17220479501905572 1101 16-17 0.021222800754635784 1101 18-19 0.20516074887947156 1101 20-21 0.33441970985968794 1101 22-23 0.3230045363660139 1101 24-25 0.34505200992938256 1101 26-27 0.5512560684240384 1101 28-29 0.5888159327769387 1101 30-31 0.08273131351236174 1101 32-33 0.5411187494326626 1101 34-35 0.8798365657819858 1101 36-37 0.8716707463205964 1101 38-39 0.6996543833204925 1101 40-41 -0.024789425700795675 1101 42-43 0.36569853754439663 1101 44-45 -0.7379514728886747 1101 46-47 -0.17864167654480667 1101 48-49 0.16847754462356335 1101 50-51 -0.23833315384067788 1101 52-53 -0.855226397233146 1101 54-55 -0.3321768137995278 1101 56-57 -0.20374363870065082 1101 58-59 0.3592171375139728 1101 60-61 -0.2596380077765801 1101 62-63 -0.12566018526220546 1101 64-65 -0.07956216038288133 1101 66-67 0.31909905238632064 1101 68-69 -0.5309325335106259 1101 70-71 -0.4965894561128934 1101 72-73 -0.5450875074040589 1101 74-75 -1.8259418187630025 1101 76-77 -0.746849511118139 1101 78-79 -0.831413437434886 1101 80-81 -1.409857839160079 1101 82-83 -0.3238709058713276 1101 84-85 -0.916601601145004 1101 86-87 -0.5323914322253067 1101 88-89 -0.8867482864664353 1101 90-91 0.4446619125031397 1101 92-93 0.015298167241404315 1101 94-95 0.2557553788771507 1101 96-97 -0.32754400319930355 1101 98-99 -0.3573727144927332 1101 100-101 -0.40397319152677014 1102 1 0.2461416627094266 1102 2 0.1596276565406285 1102 3 -0.09380272117828525 1102 4 -0.15803977840388939 1102 5 0.33835851297720154 1102 6 0.09316768977165424 1102 7 0.24836719819268183 1102 8 0.3538319649505084 1102 9 0.029617216432200166 1102 10-11 0.057220889649144624 1102 12-13 -0.33622021400045554 1102 14-15 -0.4518070626884523 1102 16-17 0.13485916439099555 1102 18-19 0.06336232990713597 1102 20-21 -0.3360348355948588 1102 22-23 -0.1819361750964319 1102 24-25 -0.33528395845006287 1102 26-27 -0.31435657979730536 1102 28-29 -0.40327892888313954 1102 30-31 -0.12626078134930196 1102 32-33 0.011968551804201866 1102 34-35 -0.413641695087577 1102 36-37 -0.2543173959718885 1102 38-39 -0.05034561667950754 1102 40-41 -0.4452439711553424 1102 42-43 0.30789221343372475 1102 44-45 0.7136295547793097 1102 46-47 0.9829195882773334 1102 48-49 0.9253945406709931 1102 50-51 -0.25315528822803657 1102 52-53 -0.22825011264816197 1102 54-55 0.689068245489004 1102 56-57 -0.03773573356231452 1102 58-59 0.06722109008314447 1102 60-61 0.6553817550692642 1102 62-63 0.2019089846982709 1102 64-65 0.6714259819096107 1102 66-67 0.8171227678013366 1102 68-69 0.0036524467265302007 1102 70-71 0.91941844902545 1102 72-73 0.8966120973390197 1102 74-75 -0.009242214019920425 1102 76-77 -0.06750168503118203 1102 78-79 0.1740213451738093 1102 80-81 -0.15738748342885245 1102 82-83 0.6489551810851957 1102 84-85 0.833892470001242 1102 86-87 0.19191686816995102 1102 88-89 -0.3659972983241424 1102 90-91 -0.3799428305798678 1102 92-93 -0.15416823592065043 1102 94-95 -0.03129995709122824 1102 96-97 -0.13287997157874898 1102 98-99 0.28986048708829415 1102 100-101 0.008082144441608818 1103 1 0.6878412674525052 1103 2 0.6457936644457618 1103 3 0.608766448782184 1103 4 0.6324740555882045 1103 5 0.5873703706847095 1103 6 0.4459344881906304 1103 7 0.5823592930543384 1103 8 0.510946589456438 1103 9 0.5968108923215283 1103 10-11 0.506825632732145 1103 12-13 0.466151327501521 1103 14-15 0.30856843138268886 1103 16-17 0.9075864371182689 1103 18-19 0.4665243852431047 1103 20-21 0.8798742553142347 1103 22-23 0.1980045363660139 1103 24-25 0.5723247372021092 1103 26-27 0.8808015229694917 1103 28-29 0.6569977509587588 1103 30-31 1.0713676771487215 1103 32-33 1.0524823857963028 1103 34-35 0.141200202145626 1103 36-37 0.78076165541151 1103 38-39 1.5291998378659457 1103 40-41 1.8161196652082978 1103 42-43 1.3770621739080369 1103 44-45 1.239321254384059 1103 46-47 1.7531765052733803 1103 48-49 1.8616593628053764 1103 50-51 1.5116668461593221 1103 52-53 1.6561372391304943 1103 54-55 1.963277731655019 1103 56-57 1.9553472703902628 1103 58-59 2.4046716829685195 1103 60-61 2.1608165376779667 1103 62-63 2.067521632919611 1103 64-65 1.6477105668898453 1103 66-67 0.8304626887499538 1103 68-69 0.9463401937621008 1103 70-71 0.8329559984325634 1103 72-73 0.3980943107777577 1103 74-75 0.4126945448733572 1103 76-77 0.09405957979095447 1103 78-79 0.4981320171105672 1103 80-81 0.6583239790217377 1103 82-83 0.18749273049230553 1103 84-85 0.8106711261277262 1103 86-87 0.9335176586837797 1103 88-89 0.351888077169928 1103 90-91 0.17193463977586276 1103 92-93 -0.4165200145767791 1103 94-95 0.6421190152407874 1103 96-97 0.1383650877097864 1103 98-99 -0.08464544176545985 1103 100-101 -0.1767004642540435 1104 1 -0.17124964163840417 1104 2 -0.33602451737241523 1104 3 -0.7903244603087209 1104 4 -0.02934412622997229 1104 5 -0.17990235658801623 1104 6 -0.8677018754457393 1104 7 -0.3994588887638386 1104 8 -0.402689774179926 1104 9 -0.608643653133015 1104 10-11 -0.5840834581769485 1104 12-13 -0.5383941270439294 1104 14-15 0.11993206774632625 1104 16-17 -0.19514083560900275 1104 18-19 -0.7262028874841704 1104 20-21 -0.44603483559485824 1104 22-23 -0.22063182727034558 1104 24-25 -0.7126752627978874 1104 26-27 -0.9578348406668624 1104 28-29 -0.9593658854048854 1104 30-31 -0.8040868683058235 1104 32-33 -0.7293357960218785 1104 34-35 -1.070163434218017 1104 36-37 -1.816056526406669 1104 38-39 -1.5003456166795104 1104 40-41 -2.2752439711553407 1104 42-43 -1.9338469170010555 1104 44-45 -3.1815878365250327 1104 46-47 -3.382732585635715 1104 48-49 -3.552431546285529 1104 50-51 -3.5492422447497702 1104 52-53 -2.7647718517785975 1104 54-55 -1.933540450163168 1104 56-57 -1.9355618205188279 1104 58-59 -2.8566919533951207 1104 60-61 -1.7641834623220305 1104 62-63 -2.031569276171293 1104 64-65 -1.806834887655608 1104 66-67 -1.316355493068226 1104 68-69 -1.6354779880560777 1104 70-71 -1.8784076379310761 1104 72-73 -0.30599659831315407 1104 74-75 -1.171850909672095 1104 76-77 -1.1818495111181413 1104 78-79 -0.8864134374348858 1104 80-81 0.43696034265810013 1104 82-83 -0.49887090587132477 1104 84-85 -1.1752379647813669 1104 86-87 -0.023300523134398787 1104 88-89 0.37052444080629243 1104 90-91 -0.5212471784059538 1104 92-93 -0.9024291054858686 1104 94-95 -0.8665173483955755 1104 96-97 -1.4602712759265764 1104 98-99 -1.2710090781290972 1104 100-101 -1.4967004642540438 1105 1 0.5537503583615973 1105 2 0.18897548262758335 1105 3 -0.07532446030872109 1105 4 0.175655873770026 1105 5 0.16009764341198007 1105 6 0.45729812455426355 1105 7 0.4755411112361614 1105 8 0.5473102258200697 1105 9 0.7513563468669844 1105 10-11 0.7034165418230529 1105 12-13 0.7116058729560706 1105 14-15 0.9949320677463263 1105 16-17 0.9973591643909927 1105 18-19 1.15629711251583 1105 20-21 0.8389651644051384 1105 22-23 0.7593681727296513 1105 24-25 0.8723247372021063 1105 26-27 0.8671651593331262 1105 28-29 0.5956341145951214 1105 30-31 1.000913131694169 1105 32-33 0.24566420397811584 1105 34-35 0.17983656578198293 1105 36-37 0.3489434735933301 1105 38-39 0.47465438332049814 1105 40-41 -0.13274397115534242 1105 42-43 -0.6638469170010595 1105 44-45 -0.6015878365250344 1105 46-47 -0.7627325856357103 1105 48-49 -1.0474315462855301 1105 50-51 -0.5792422447497714 1105 52-53 -0.24727185177859923 1105 54-55 -1.3810404501631695 1105 56-57 -1.2855618205188293 1105 58-59 -0.706691953395115 1105 60-61 -0.6141834623220319 1105 62-63 -0.8290692761712926 1105 64-65 -1.8568348876556087 1105 66-67 -1.6513554930682268 1105 68-69 -1.5729779880560777 1105 70-71 -2.5909076379310747 1105 72-73 -2.848496598313151 1105 74-75 -3.729350909672096 1105 76-77 -3.934349511118139 1105 78-79 -2.9064134374348853 1105 80-81 -2.615539657341902 1105 82-83 -3.661370905871326 1105 84-85 -3.3177379647813687 1105 86-87 -2.7233005231344016 1105 88-89 -2.9344755591937073 1105 90-91 -2.521247178405954 1105 92-93 -3.507429105485869 1105 94-95 -3.159017348395576 1105 96-97 -2.3252712759265783 1105 98-99 -2.946009078129098 1105 100-101 -2.4267004642540435 1106 1 0.1809242714050754 1106 2 0.24658417827975398 1106 3 0.1888059744738868 1106 4 0.12456891724828267 1106 5 0.33835851297720154 1106 6 0.22360247238034958 1106 7 0.37880198080137717 1106 8 0.0929623997331177 1106 9 -0.12255669661127655 1106 10-11 0.2637426287795748 1106 12-13 0.34856239469519323 1106 14-15 0.37427989383328253 1106 16-17 -0.1586190964785672 1106 18-19 0.313362329907136 1106 20-21 0.490052120926876 1106 22-23 0.4811073031644355 1106 24-25 0.6103682154629766 1106 26-27 0.25086081150704587 1106 28-29 0.4010688972038139 1106 30-31 -0.11539121613191838 1106 32-33 -0.031509709065367986 1106 34-35 -0.07668517334845149 1106 36-37 0.017421734462892857 1106 38-39 -0.3220847471142889 1106 40-41 0.4460603766707507 1106 42-43 0.21006612647720146 1106 44-45 0.09406433738801212 1106 46-47 -0.6040369334617992 1106 48-49 -0.3137358941116233 1106 50-51 -0.36185094040194343 1106 52-53 -0.48911967786555266 1106 54-55 -0.593540450163168 1106 56-57 -0.6790400813883934 1106 58-59 -1.378431083829902 1106 60-61 -0.7576617231915961 1106 62-63 -0.9720040587799907 1106 64-65 -0.33944358330778 1106 66-67 -0.34592071045953077 1106 68-69 -1.289825814143036 1106 70-71 -1.8523206814093314 1106 72-73 -1.1794748591827187 1106 74-75 -0.5527204748894867 1106 76-77 -0.871849511118139 1106 78-79 -0.7390221330870617 1106 80-81 -0.603039657341899 1106 82-83 -0.05756655804524158 1106 84-85 -0.035672747390062653 1106 86-87 -0.6885179144387479 1106 88-89 0.10139400602368198 1106 90-91 0.35918760420274154 1106 92-93 0.30235350320978327 1106 94-95 -0.7812999570912282 1106 96-97 0.7910330718995091 1106 98-99 1.3876865740448174 1106 100-101 2.2906908400937844 1107 1 0.0378412674525066 1107 2 -0.5542063355542375 1107 3 -0.459415369399629 1107 4 -0.15843503532088477 1107 5 -0.12172053840620123 1107 6 0.21411630637244627 1107 7 -0.4812770705820242 1107 8 0.09731022582007398 1107 9 0.17862907413970674 1107 10-11 0.2295529054594141 1107 12-13 0.6343331456833425 1107 14-15 0.6335684313826917 1107 16-17 0.9075864371182689 1107 18-19 0.42561529433401546 1107 20-21 0.45941970985968794 1107 22-23 0.8139136272751131 1107 24-25 0.4245974644748429 1107 26-27 0.6285287956967665 1107 28-29 0.5024522964133027 1107 30-31 0.39409494987599203 1107 32-33 0.05248238579630282 1107 34-35 0.3934729294183512 1107 36-37 1.0103071099569618 1107 38-39 1.6996543833204925 1107 40-41 1.4956651197537454 1107 42-43 0.9452439920898499 1107 44-45 0.42568489074769644 1107 46-47 1.4645401416370163 1107 48-49 0.5139320900781073 1107 50-51 0.0980304825229581 1107 52-53 0.40159178458503675 1107 54-55 0.551914095291373 1107 56-57 0.13716545220844267 1107 58-59 0.7887625920594274 1107 60-61 0.9767256285870545 1107 62-63 1.2629761783741564 1107 64-65 1.8022560214353014 1107 66-67 1.600917234204502 1107 68-69 1.678158375580285 1107 70-71 1.4215923620689281 1107 72-73 1.818548856232301 1107 74-75 1.7354218176006313 1107 76-77 1.1145141252454955 1107 78-79 1.134495653474204 1107 80-81 1.3742330699308276 1107 82-83 0.946583639583217 1107 84-85 1.367489307945906 1107 86-87 0.9085176586837811 1107 88-89 0.4041608044426539 1107 90-91 1.1151164579576829 1107 92-93 1.099389076332315 1107 94-95 0.6080281061498773 1107 96-97 1.4406378149825123 1107 98-99 1.7880818309618114 1107 100-101 1.1642086266550464 1108 1 -0.15124964163840104 1108 2 -0.09602451737242035 1108 3 0.4696755396912806 1108 4 0.09065587377002515 1108 5 -0.43990235658801424 1108 6 -0.4677018754457336 1108 7 -0.2994588887638443 1108 8 0.05731022582006773 1108 9 -0.16864365313301732 1108 10-11 -0.36408345817694965 1108 12-13 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64-65 -3.6216497024704246 2316 66-67 -3.2016641350435364 2316 68-69 -3.534860704105462 2316 70-71 -3.99322245274589 2316 72-73 -3.864391660041548 2316 74-75 -3.3844435022646877 2316 76-77 -3.393454449389747 2316 78-79 -4.055178869533652 2316 80-81 -3.414768052403627 2316 82-83 -3.476648683649106 2316 84-85 -3.1706700635467975 2316 86-87 -3.6387326218998304 2316 88-89 -3.344660744378892 2316 90-91 -4.082975573467682 2316 92-93 -3.3392192289426603 2316 94-95 -3.111949447161006 2316 96-97 -3.195950288272254 2316 98-99 -3.424712781832799 2316 100-101 -3.321762192649107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 18.0 15 54.0 16 207.0 17 558.0 18 797.0 19 996.0 20 1317.0 21 1777.0 22 1789.0 23 1291.0 24 1244.0 25 1269.0 26 1450.0 27 1798.0 28 2239.0 29 2328.0 30 1324.0 31 897.0 32 852.0 33 1186.0 34 1957.0 35 2522.0 36 3957.0 37 8365.0 38 10326.0 39 3333.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.65799981428174 17.388801188596897 12.911133810010215 23.042065187111152 2 12.957144185181743 21.42047039137306 46.637538347122806 18.984847076322396 3 18.200724166744035 33.14084114752576 34.05440534769288 14.604029338037321 4 10.275740414074832 23.98106025438678 31.974747005849043 33.76845232568935 5 8.805124872342402 42.08894253086993 34.30507845139727 14.8008541453904 6 21.28623693056252 39.89265882964696 19.04284361245752 19.778260627333 7 21.286356376063296 36.16507559154563 18.717729653430407 23.83083837896066 8 27.015133228112525 32.8511744499118 21.570884783214186 18.56280753876149 9 29.00009284189026 12.30712097298301 15.926097855352337 42.7666883297744 10-11 22.42967226812738 28.737350292451953 26.552780614613315 22.28019682480735 12-13 27.83956921362919 24.933618048463465 26.493361804846348 20.733450933060997 14-15 25.42660848574877 24.47869278618513 23.720174542753693 26.374524185312413 16-17 21.930182898523814 30.483706248259214 25.916813666326245 21.669297186890727 18-19 22.50116052362826 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34.862129792962584 18.875684708940675 44-45 17.34193668183084 28.523813944851916 34.3394299507938 19.794819422523442 46-47 20.952557794076686 24.627239810602543 30.879212700770587 23.54098969455018 48-49 20.44099897873921 24.909479156995637 29.890446569492156 24.759075294773 50-51 16.638195153653328 24.334787856280755 34.193668183084206 24.833348806981707 52-53 16.362454739578496 25.284560393649613 36.899080865286415 21.453904001485473 54-55 16.3940209822672 25.603008077244453 31.4464766502646 26.556494290223746 56-57 20.61182805681924 26.761674867700307 27.303871506823878 25.322625568656576 58-59 16.53978274997679 28.380837433850154 33.36551852195711 21.71386129421595 60-61 20.386222263485283 32.147432921734286 24.983752669204346 22.48259214557608 62-63 16.239125792644998 35.93571567835557 24.892070300531987 22.933088228467444 64-65 15.553801875406185 35.64293009005664 26.408875684708942 22.394392349828244 66-67 16.436728251787205 34.520471636802526 26.420945130442856 22.621854980967413 68-69 18.365982731408412 32.09822671989602 26.718039179277692 22.817751369417884 70-71 18.53959706619627 31.179092006313248 27.208244359855165 23.073066567635315 72-73 17.917556401448334 30.51898616655835 26.300250673103704 25.263206758889613 74-75 18.829263763810232 29.529291616377307 26.665119301828987 24.976325317983473 76-77 18.935103518707642 28.513601336923223 28.7735586296537 23.77773651471544 78-79 18.76613127843283 27.887846996564853 28.348342772258846 24.997678952743478 80-81 18.79027016990066 28.27685451675796 27.7885061739857 25.14436913935568 82-83 18.57951907900845 28.002970940488346 27.23516850803082 26.18234147247238 84-85 18.80605329124501 27.7773651471544 27.003992201281218 26.412589360319377 86-87 19.424380280382508 27.74301364775787 25.997586110853216 26.835019961006406 88-89 19.628632438956455 26.175842540154115 26.707826571348992 27.48769844954043 90-91 19.26005013462074 27.045771051898615 26.213907715161085 27.480271098319562 92-93 19.375174078544237 27.136756104354287 25.566799740042708 27.921270077058768 94-95 18.644508402191068 26.00501346207409 26.441370346300253 28.90910778943459 96-97 19.248909107789437 25.40525485098877 26.199053012719336 29.14678302850246 98-99 19.619530401359217 25.278296149811062 25.77779015681141 29.324383292018307 100-101 20.168605568811685 25.794052382853483 25.256482865551916 28.78085918278292 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 28.5 2 35.0 3 59.0 4 35.0 5 24.5 6 43.5 7 68.5 8 103.0 9 111.5 10 82.5 11 53.5 12 48.5 13 52.0 14 57.0 15 53.5 16 56.0 17 75.5 18 87.0 19 92.5 20 108.5 21 126.0 22 151.0 23 191.0 24 208.0 25 263.0 26 329.0 27 377.5 28 443.5 29 510.5 30 602.0 31 692.0 32 786.0 33 950.0 34 1086.5 35 1524.5 36 1906.5 37 1920.0 38 1982.0 39 1898.0 40 1855.5 41 1823.5 42 1736.5 43 1748.0 44 1686.5 45 1777.5 46 1975.0 47 2032.0 48 2449.5 49 2694.0 50 2988.0 51 3122.5 52 2181.0 53 1419.0 54 1206.0 55 1092.5 56 887.0 57 719.5 58 604.5 59 463.0 60 418.5 61 387.0 62 374.0 63 368.0 64 287.5 65 133.0 66 44.0 67 35.0 68 29.5 69 16.0 70 10.0 71 11.0 72 12.0 73 8.0 74 2.5 75 1.5 76 4.5 77 4.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01856837805217714 2 0.12997864636523998 3 0.0 4 0.0 5 0.0 6 0.014854702441741714 7 0.024138891467830284 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.005570513415653143 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 9.284189026088571E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 9.284189026088571E-4 100-101 0.0027852567078265715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 53855.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.408411475257637 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86166124741354 23.82694271655371 2 8.826485368016554 5.544517686380095 3 3.83683121489802 3.6152632067588897 4 2.0928170263080106 2.6292823321882834 5 1.5784806384865502 2.4788784699656485 6 1.0523204256577001 1.9831027759725186 7 0.8867868755542417 1.9496796954786 8 0.6916937629323087 1.7380001856837806 9 0.532072125332545 1.5040386222263484 >10 4.085131540053207 24.716368025252994 >50 0.37245048773278155 8.127379073437934 >100 0.15370972509606856 8.240646179556215 >500 0.017735737511084836 4.255872249559001 >1k 0.01182382500738989 9.39002878098598 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 3781 7.0207037415281786 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 1276 2.369325039457803 TruSeq Adapter, Index 1 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 909 1.6878655649429024 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 718 1.3332095441463188 RNA PCR Primer, Index 1 (95% over 23bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 665 1.23479714046978 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.7724445269705691 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 339 0.6294680159688051 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 294 0.545910314734008 No Hit CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 258 0.4790641537461703 TruSeq Adapter, Index 1 (96% over 26bp) GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 223 0.41407483056355027 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 184 0.34165815616005946 TruSeq Adapter, Index 1 (96% over 28bp) GTACATGGGATGTGGACACCTGAATATGCAAGGTGTCTTCCTGAAAAGCC 183 0.3398013183548417 No Hit TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 162 0.3008077244452697 No Hit CATTAAATCAAATGGAGGGAGGGTGTGAAGGAAAAGGGAGCTGAGACCAG 153 0.2840961841983103 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 152 0.28223934639309256 No Hit CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG 151 0.28038250858787483 TruSeq Adapter, Index 1 (95% over 24bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.26924148175656853 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC 144 0.26738464395135086 No Hit TGTAAGAGGAGATCAGCTGCTCTTCACCACAATTACTACCAAATACTAGG 140 0.25995729273047996 No Hit GTGAGAGGCTAATCCTTTCCATCTCAGGGCTATCACATTCACACACACAT 139 0.25810045492526223 No Hit TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT 138 0.25624361712004456 No Hit CTTTTATGTTGCAAATGTAATAAACTGACATGCCTTTGACTTTCACACAC 135 0.25067310370439144 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 132 0.2451025902887383 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 0.23953207687308514 No Hit GTACATGGGGATCAAAGACGCAGATCAGGCAGGACTTCCAGTGGAAGCAA 125 0.23210472565221427 No Hit GTACAATGGGGGGGTGTTACCAAGGCTGCAGAGAAGCAGATGAAGGACAA 123 0.22839105004177884 No Hit GTACATGGGGGGACAAGCGAATAAGTGCACATGGTGGATGCCTTGGCGAT 120 0.2228205366261257 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.21725002321047257 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 117 0.21725002321047257 No Hit GTACATGGGAGAAGTCTGTGGCTTTGTCAGATATGTCACCCAAGTAAATG 111 0.20610899637916627 No Hit ACATTGGGGTCTGTCAGAACAAGACATCTGCTCATATGAAGCCAGACTCA 108 0.20053848296351312 No Hit GTAATAAACTGACATGCCTTTGACTTTCACACACTCACAACAAGATCATT 100 0.18568378052177142 No Hit CCCTAAAAGGGTGGGTTGCCCCATTCGGAAATCTGCGGATCAAAGTGTGT 97 0.18011326710611827 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 96 0.17825642930090058 No Hit TTACATAACTCTGCAGTGTTTCTCAAGTGAGCTTTTCATTTTAATCAGAT 95 0.17639959149568285 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 93 0.17268591588524743 No Hit GAGTACAATGGGGGGGTGTTACCAAGGCTGCAGAGAAGCAGATGAAGGAC 92 0.17082907808002973 No Hit CCCCAATGTCATGCCTACAGTCCAAACACACACCCAGCCTCTGGGAAAAC 91 0.16897224027481197 No Hit GTGTTTGGACTGTAGGCATGACATTGGGGTCTGTCAGAACAAGACATCTG 91 0.16897224027481197 No Hit GGCTAATCCTTTCCATCTCAGGGCTATCACATTCACACACACATTAAATC 90 0.16711540246959428 No Hit TCTCTGTACAATGTCGATTATAATTATGCAAACAACAAAACTGTCAGTCT 89 0.16525856466437658 No Hit CATTTCAAGTGGTTGGCCATGTAAGAGGAGATCAGCTGCTCTTCACCACA 89 0.16525856466437658 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.15783121344350573 No Hit CCTGAATATGCAAGGTGTCTTCCTGAAAAGCCCACACATTTCAAGTGGTT 85 0.15783121344350573 No Hit GTGTAAGCTTTTAAAGTTGTAGGTTAAACCTCCTGTTGTTATCTTTGCTG 82 0.15226070002785258 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 82 0.15226070002785258 No Hit GTACATGGGCCACTAAAAATACAAAAATTAGCTGGGTGTGGTGGCGTGCA 81 0.15040386222263485 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 79 0.14669018661219943 No Hit GTACAATGTCGATTATAATTATGCAAACAACAAAACTGTCAGTCTGTTAG 79 0.14669018661219943 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 78 0.14483334880698173 No Hit GTCCAAACACACACCCAGCCTCTGGGAAAACACACCCTGCAAGCTTCTGG 78 0.14483334880698173 No Hit GTTTTACAACATCAGCCCATGATTTTGCAACAATCAAGTTGGCTTCAGAA 78 0.14483334880698173 No Hit GTACTTTTATGTTGCAAATGTAATAAACTGACATGCCTTTGACTTTCACA 75 0.13926283539132858 No Hit ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC 74 0.13740599758611086 Illumina PCR Primer Index 5 (95% over 24bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 73 0.13554915978089313 No Hit ACCAAATACTAGGACTAGTTCCTGCTGTTGCTCTCGAAAGTGCCATATTC 71 0.1318354841704577 No Hit TCTTTATCCTCAGCTTCTTCTTCCTCTTTACGCTTCTTGTCCTCTTCTTC 71 0.1318354841704577 No Hit GTACATGGGGGGGGGATGGAAAGAAGCCAACCAGCTGCTGACAAGCCAGA 71 0.1318354841704577 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 69 0.12812180856002228 No Hit GTCTGAGGGTGTGCAGGTTCCCTCTGTGCCCATCCAGCAGTTCCCCACGG 69 0.12812180856002228 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 67 0.12440813294958684 No Hit GTAGAATGCCATGCTGACTCCGGCTGATGGATGCTCTCTTCGCAGGCAGG 65 0.12069445733915143 No Hit CCTTAAGTCACTGAATTAAGGGTTAAAAAAAGTTTAAAGAGGTGGCTACC 65 0.12069445733915143 No Hit GAATTAAGGGTTAAAAAAAGTTTAAAGAGGTGGCTACCACATTCCAGATA 64 0.11883761953393371 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.116980781728716 No Hit GAGTACATGGGGGGACAAGCGAATAAGTGCACATGGTGGATGCCTTGGCG 63 0.116980781728716 No Hit CCCTCAGACCAGTCGGCCACCTCAGGCTGAGCAGCAGTGAACTCAGGAGC 63 0.116980781728716 No Hit TCTCAGGGCTATCACATTCACACACACATTAAATCAAATGGAGGGAGGGT 62 0.11512394392349828 No Hit GACTAGTTCCTGCTGTTGCTCTCGAAAGTGCCATATTCCTGAGTCTGGCT 62 0.11512394392349828 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 62 0.11512394392349828 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 61 0.11326710611828056 No Hit GACTAAAACTTCCAGTACAACGTTGAATAGAAGTGAGAGTAGATTCCCTG 61 0.11326710611828056 No Hit AGTAAATGCTTTGTAGATCTGATTAAAATGAAAAGCTCACTTGAGAAACA 60 0.11141026831306285 No Hit ATATAGGGACATATCTGCTAAAAGAACCCATGAATTTTTCAGATAGTGTC 60 0.11141026831306285 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.10955343050784515 No Hit GTGGATGCCTTGGCGATTACAGGCGATGAAGGACGTAGTAGCTTGCGATA 58 0.10769659270262744 No Hit GTACATGGGGATGTGGTGGATGCTGGCCAGGGAAGTACTCCGCATGCGAG 58 0.10769659270262744 No Hit CATTAGGATAGGGTAGGCTAAGTTCCCTATGCTGCTAGCATCCTAAGATG 57 0.10583975489740971 No Hit TTTTTATTCTTTGTGTACAAAGACTAGCATGATCGGTTCCACTTGGTAGT 57 0.10583975489740971 No Hit GACCAGAAGCTTGCAGGGTGTGTTTTCCCAGAGGCTGGGTGTGTGTTTGG 57 0.10583975489740971 No Hit TACTAGGACTAGTTCCTGCTGTTGCTCTCGAAAGTGCCATATTCCTGAGT 57 0.10583975489740971 No Hit CAATATAGGGACATATCTGCTAAAAGAACCCATGAATTTTTCAGATAGTG 56 0.103982917092192 No Hit CCTATGTATTCAATATGCAATACCCTAAAAGGGTGGGTTGCCCCATTCGG 56 0.103982917092192 No Hit GTATCAACGCAGAGTACATGGGGATCAAAGACGCAGATCAGGCAGGACTT 55 0.10212607928697429 No Hit GCTCAGGACCACTCAGTGGTGGCTCCAACCCACTCAGTGGCCTGCGCTGT 55 0.10212607928697429 No Hit GTGTATCTCCAAACCTTAAGTCACTGAATTAAGGGTTAAAAAAAGTTTAA 54 0.10026924148175656 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 54 0.10026924148175656 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0018568378052177142 0.0 5 0.0 0.0 0.0 0.0018568378052177142 0.0 6 0.0 0.0 0.0 0.0018568378052177142 0.0 7 0.0 0.0 0.0 0.0018568378052177142 0.0 8 0.0 0.0 0.0 0.0018568378052177142 0.0 9 0.0 0.0 0.0 0.0018568378052177142 0.0 10-11 0.0 0.0 0.0 0.004642094513044285 0.0 12-13 0.0 0.0 0.0 0.007427351220870857 0.0 14-15 0.0 0.0 0.0 0.015783121344350573 0.0 16-17 0.0 0.0 0.0 0.01856837805217714 0.0 18-19 0.0 0.0 0.0 0.020425215857394857 0.0 20-21 0.0 0.0 0.0 0.025995729273047996 0.0 22-23 0.0 0.0 0.0 0.05570513415653143 0.0 24-25 0.0 0.0 0.0 0.09469872806610342 0.0 26-27 0.0 0.0 0.0 0.18011326710611827 0.0 28-29 0.0 0.0 0.0 0.27388357626961285 0.0 30-31 0.0 0.0 0.0 0.43171478971311855 0.0 32-33 0.0 0.0 0.0 0.6554637452418531 0.0 34-35 0.0 0.0 0.0 1.0936774672732337 0.0 36-37 0.0 0.0 0.0 1.5402469594280939 0.0 38-39 0.0 0.0 0.0 2.0889425308699283 0.0 40-41 0.0 0.0 0.0 2.8901680438213724 0.0 42-43 0.0 0.0 0.0 3.4500046420945134 0.0 44-45 0.0 0.0 0.0 3.830656392164144 0.0 46-47 0.0 0.0 0.0 4.246588060532913 0.0 48-49 0.0 0.0 0.0 4.6875870392721195 0.0 50-51 0.0 0.0 0.0 5.185219571070467 0.0 52-53 0.0 0.0 0.0 5.7088478321418625 0.0 54-55 0.0 0.0 0.0 6.1656299322254196 0.0 56-57 0.0 0.0 0.0 6.53885433107418 0.0 58-59 0.0 0.0 0.0 6.908365054312506 0.0 60-61 0.0 0.0 0.0 7.250023210472565 0.0 62-63 0.0 0.0 0.0 7.588896109924798 0.0 64-65 0.0 0.0 0.0 7.9676910221892125 0.0 66-67 0.0 0.0 0.0 8.356698542382322 0.0 68-69 0.0 0.0 0.0 8.828335344907622 0.0 70-71 0.0 0.0 0.0 9.372388821836413 0.0 72-73 0.0 0.0 0.0 9.8950886640052 0.0 74-75 0.0 0.0 0.0 10.355584439699193 0.0 76-77 0.0 0.0 0.0 10.83743385015319 0.0 78-79 0.0 0.0 0.0 11.314641166094141 0.0 80-81 0.0 0.0 0.0 11.895831399127285 0.0 82-83 0.0 0.0 0.0 12.495590010212608 0.0 84-85 0.0 0.0 0.0 13.066567635317055 0.0 86-87 0.0 0.0 0.0 13.64682944944759 0.0 88-89 0.0 0.0 0.0 14.278154303221614 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGCTA 15 6.5836805E-4 94.99907 5 TGCCCTG 20 0.002063405 71.249306 5 GCTAATC 20 0.002063405 71.249306 8 CTAATCC 20 0.002063405 71.249306 9 GTACCCA 25 0.004977226 57.052418 1 ACTTGAC 25 0.0049955854 56.999447 2 CCTGACT 25 0.0049955854 56.999447 8 ATTGCCC 25 0.0049955854 56.999447 3 GGCTAAT 25 0.0049955854 56.999447 7 CTTGACT 25 0.0049955854 56.999447 3 GACTTTA 25 0.0049955854 56.999447 6 AATTGCC 25 0.0049955854 56.999447 2 TTGCCCT 25 0.0049955854 56.999447 4 TGAGAGG 25 0.0049955854 56.999447 2 TATGTTG 40 5.342538E-4 47.499535 5 TGGGATG 60 1.4658417E-6 47.499535 6 ACAATGG 40 5.342538E-4 47.499535 3 ATAAGTG 45 9.543903E-4 42.22181 5 TAAGTGC 45 9.543903E-4 42.22181 6 TTATGTT 45 9.543903E-4 42.22181 4 >>END_MODULE