FastQCFastQC Report
Fri 10 Feb 2017
SRR2568705_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568705_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815634
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8470.10384559741256495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.040.720911
GTATCAA16700.034.137291
ATCAACG16400.032.438333
TCAACGC16550.032.1443334
CAACGCA16600.032.047515
AACGCAG16950.031.3857636
TACGCTG650.00584369929.2301465
TATCAAC18700.029.2159822
CATTGCG700.00839103727.1422799
ACGCAGA19400.026.9324157
CGCAGAG19750.026.2146328
GGGGCGT950.001226401424.9994686
GTACATG24450.024.2882041
ACATGGG22700.023.4356233
TAGCCCT8400.023.184034
TACATGG24500.023.0751822
ATAGACC2955.456968E-1222.5418933
TTAGGCG1100.002877012421.590459
GTAGCCC5150.021.2131423
GTTACAC3850.020.973583
Produced by FastQC (version 0.11.5)