##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568705_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 815634 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01420612676765 33.0 31.0 34.0 30.0 34.0 2 32.16610636633588 34.0 31.0 34.0 30.0 34.0 3 32.204418893768526 34.0 31.0 34.0 30.0 34.0 4 35.5400915116339 37.0 35.0 37.0 33.0 37.0 5 35.59425428562321 37.0 35.0 37.0 33.0 37.0 6 35.71336653449954 37.0 36.0 37.0 35.0 37.0 7 35.71568865447002 37.0 36.0 37.0 35.0 37.0 8 35.738032009455225 37.0 36.0 37.0 35.0 37.0 9 37.51480075622154 39.0 38.0 39.0 35.0 39.0 10-11 37.497386695503124 39.0 38.0 39.0 35.0 39.0 12-13 37.47098392170999 39.0 38.0 39.0 35.0 39.0 14-15 38.87973220831893 41.0 39.0 41.0 35.0 41.0 16-17 38.87142517354598 41.0 39.0 41.0 35.0 41.0 18-19 38.86772253241037 41.0 39.0 41.0 35.0 41.0 20-21 38.88599543422663 41.0 39.0 41.0 35.0 41.0 22-23 38.82069715092799 41.0 39.0 41.0 35.0 41.0 24-25 38.77365766998433 41.0 39.0 41.0 34.0 41.0 26-27 38.69693637097031 41.0 39.0 41.0 34.0 41.0 28-29 38.58039635424713 40.5 38.5 41.0 34.0 41.0 30-31 38.501623277107136 40.0 38.0 41.0 34.0 41.0 32-33 38.39705063790867 40.0 38.0 41.0 34.0 41.0 34-35 38.25947790307907 40.0 38.0 41.0 33.5 41.0 36-37 38.16461182344041 40.0 38.0 41.0 33.0 41.0 38-39 38.08110745751158 40.0 38.0 41.0 33.0 41.0 40-41 37.96558628012074 40.0 38.0 41.0 33.0 41.0 42-43 37.82569939458139 40.0 38.0 41.0 32.5 41.0 44-45 37.67141266793685 40.0 38.0 41.0 32.0 41.0 46-47 37.72586356135227 40.0 38.0 41.0 32.5 41.0 48-49 37.88665075266603 40.0 38.0 41.0 33.0 41.0 50-51 36.81258628257282 39.0 36.5 40.0 31.0 40.5 52-53 36.97080185475348 39.5 37.0 40.0 31.0 40.5 54-55 37.608434665548515 40.0 38.0 41.0 32.0 41.0 56-57 37.62986278159076 40.0 37.5 41.0 32.0 41.0 58-59 37.51285870868551 40.0 37.0 41.0 31.5 41.0 60-61 37.36231998666068 40.0 37.0 41.0 31.0 41.0 62-63 37.16386271293251 40.0 36.0 41.0 31.0 41.0 64-65 37.04107295674285 39.5 36.0 41.0 31.0 41.0 66-67 36.971417939909315 39.0 36.0 41.0 31.0 41.0 68-69 36.72904636148076 39.0 35.0 41.0 31.0 41.0 70-71 36.393662476061564 39.0 35.0 41.0 30.0 41.0 72-73 36.05488981577521 37.5 35.0 40.0 30.0 41.0 74-75 35.68998411052016 37.0 35.0 39.5 30.0 41.0 76-77 35.2822859272664 37.0 35.0 39.0 29.0 41.0 78-79 34.874381156253904 36.0 35.0 39.0 29.0 39.5 80-81 34.4490335125804 36.0 34.0 37.5 29.0 39.0 82-83 34.073536659825365 35.0 34.0 37.0 29.0 39.0 84-85 33.71150908373119 35.0 34.0 37.0 29.0 38.0 86-87 33.425255690665175 35.0 34.0 36.0 28.0 37.0 88-89 33.18056076622603 35.0 34.0 36.0 28.0 37.0 90-91 32.98161062437319 35.0 34.0 35.5 27.0 36.5 92-93 32.80334745731541 35.0 34.0 35.0 27.0 36.0 94-95 32.65095802774283 35.0 34.0 35.0 27.0 36.0 96-97 32.55187988730239 35.0 33.5 35.0 26.0 36.0 98-99 32.42788554670355 35.0 33.0 35.0 26.0 35.5 100-101 31.218527550347336 34.5 31.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06622598063650997 1101 2 -0.030109068263769245 1101 3 0.26759379959130314 1101 4 0.05616158030604623 1101 5 -0.08839263763743332 1101 6 -0.07885019400774951 1101 7 -2.5136217198706845E-4 1101 8 -0.18724591732791396 1101 9 -0.0088212767673852 1101 10-11 -0.04588923236445197 1101 12-13 0.028845025172806515 1101 14-15 0.1580710039487201 1101 16-17 0.17663058772047435 1101 18-19 0.1754422121551329 1101 20-21 0.03560270042878955 1101 22-23 -0.020991059738271645 1101 24-25 -0.0010109766488852756 1101 26-27 0.06802672154585565 1101 28-29 0.022333423884198567 1101 30-31 0.1514062764102988 1101 32-33 0.08613622713562563 1101 34-35 -0.06323953285233586 1101 36-37 0.0925449124726967 1101 38-39 0.07323567808087006 1101 40-41 0.1057885045728355 1101 42-43 0.04977697089651656 1101 44-45 0.17810926108960246 1101 46-47 0.20329046537455042 1101 48-49 0.08307439131399974 1101 50-51 -0.048515597829535295 1101 52-53 0.06422679260148101 1101 54-55 -0.048616418411661755 1101 56-57 -0.08105688487026441 1101 58-59 -0.022486408538341607 1101 60-61 0.07905397265103176 1101 62-63 0.09467935633777103 1101 64-65 0.09060422313381622 1101 66-67 0.18444571003139032 1101 68-69 0.014948861733728336 1101 70-71 0.09855421462975045 1101 72-73 0.008192299224916155 1101 74-75 0.08970500140254956 1101 76-77 0.04081113357674582 1101 78-79 -0.10369913989175927 1101 80-81 -0.08454938284119606 1101 82-83 0.047435261867796896 1101 84-85 -0.02256289079083018 1101 86-87 -0.2066818752979671 1101 88-89 -0.18212332915914686 1101 90-91 -0.06230711815576484 1101 92-93 -0.1026659520107458 1101 94-95 -0.04445677577676577 1101 96-97 -0.10913580343751761 1101 98-99 -0.05643259289004021 1101 100-101 -0.019061511521574204 1102 1 0.41101142207715213 1102 2 0.32293451916493865 1102 3 0.4907409784734895 1102 4 0.4746223121110802 1102 5 0.4730671322865234 1102 6 0.3187500880948022 1102 7 0.4442244680273859 1102 8 0.5470330503392233 1102 9 0.5579186848681488 1102 10-11 0.4881338490889604 1102 12-13 0.4477174642336692 1102 14-15 0.5831827464673651 1102 16-17 0.6505185627300278 1102 18-19 0.520524895157159 1102 20-21 0.5614202531891692 1102 22-23 0.5364781037575099 1102 24-25 0.6296146891445247 1102 26-27 0.5572420222860117 1102 28-29 0.5857114173005655 1102 30-31 0.5347721955421321 1102 32-33 0.6549854961244677 1102 34-35 0.546962314476275 1102 36-37 0.3587690399948613 1102 38-39 0.3643690176913523 1102 40-41 0.4807402720523939 1102 42-43 0.3629421873566798 1102 44-45 0.3836407234626904 1102 46-47 0.49211510577687534 1102 48-49 0.5386487783225178 1102 50-51 0.4965164987204318 1102 52-53 0.36543865198591163 1102 54-55 0.53255545182855 1102 56-57 0.5031040169717045 1102 58-59 0.4477635882307709 1102 60-61 0.43787807764930875 1102 62-63 0.4617939161894853 1102 64-65 0.5634195438131613 1102 66-67 0.579316354565556 1102 68-69 0.44706701248505 1102 70-71 0.4549726938237626 1102 72-73 0.25727703522456125 1102 74-75 0.4539856122062673 1102 76-77 0.2738291323840869 1102 78-79 0.3679678314013586 1102 80-81 0.5851090847585496 1102 82-83 0.5767523263027599 1102 84-85 0.46583545721072994 1102 86-87 0.44517809992574087 1102 88-89 0.3445499469782547 1102 90-91 0.39838960867923845 1102 92-93 0.3460543399875533 1102 94-95 0.2861847675161968 1102 96-97 0.38621818172256184 1102 98-99 0.2941065959174267 1102 100-101 0.35575086628167796 1103 1 0.2899156479092895 1103 2 0.30239483960620106 1103 3 0.2686943833792199 1103 4 0.1799373802231088 1103 5 0.2937970455858405 1103 6 0.3477616029744226 1103 7 0.2561208584551906 1103 8 0.13303991544715643 1103 9 0.3032819548018253 1103 10-11 0.3756742346339834 1103 12-13 0.4280698313743514 1103 14-15 0.27463718253040525 1103 16-17 0.3534896172926807 1103 18-19 0.23338236591785488 1103 20-21 0.1403655166472504 1103 22-23 0.3194003652784758 1103 24-25 0.32276801336611527 1103 26-27 0.3076828289883551 1103 28-29 0.20935291538258838 1103 30-31 0.26296400123245434 1103 32-33 0.39593461294607124 1103 34-35 0.2841302313994234 1103 36-37 0.1628387204926014 1103 38-39 0.10327842539320642 1103 40-41 0.2102562367322136 1103 42-43 0.09459784689740758 1103 44-45 0.21737139143699835 1103 46-47 0.41673194302791217 1103 48-49 0.2688098683931486 1103 50-51 0.3581979632767869 1103 52-53 0.2923108197811146 1103 54-55 0.575366737871235 1103 56-57 0.44242223593874996 1103 58-59 0.425124208107583 1103 60-61 0.33840095960353267 1103 62-63 0.31468446049446896 1103 64-65 0.29257729870723637 1103 66-67 0.1857376996954727 1103 68-69 0.32146144667008514 1103 70-71 0.20346856050016981 1103 72-73 -0.012096723648085117 1103 74-75 0.0276177203230219 1103 76-77 0.08247381262099651 1103 78-79 0.10801968487616875 1103 80-81 0.13818325505079088 1103 82-83 0.10462574261556057 1103 84-85 0.18010403108367257 1103 86-87 0.1837701615795595 1103 88-89 0.1509627715841404 1103 90-91 -0.06946888802209372 1103 92-93 0.03677322877209832 1103 94-95 0.0059308211224617935 1103 96-97 0.03318244073258114 1103 98-99 0.2440267812127459 1103 100-101 0.11363061212662018 1104 1 0.007889914397935627 1104 2 0.14545629048734554 1104 3 0.12208510895264624 1104 4 0.26593938629446257 1104 5 0.15656382982688655 1104 6 0.0014319828658813094 1104 7 0.08761418594301773 1104 8 0.11929965518928753 1104 9 0.005712489599190462 1104 10-11 0.13435115750651505 1104 12-13 0.05876504802341742 1104 14-15 0.26636545950110246 1104 16-17 0.19057104917592937 1104 18-19 0.14316395655972514 1104 20-21 0.03835147414776685 1104 22-23 -0.0017769727578311745 1104 24-25 0.23059833427110732 1104 26-27 0.23345652819721607 1104 28-29 0.15802040573417742 1104 30-31 0.2715130536626589 1104 32-33 0.2545713479653031 1104 34-35 0.13512795298063907 1104 36-37 -0.02305168955635395 1104 38-39 -0.1114143866272812 1104 40-41 -0.0017795271359162257 1104 42-43 -0.1341739990203692 1104 44-45 0.18483749746651057 1104 46-47 0.17127051340315802 1104 48-49 0.2122147427479888 1104 50-51 0.08111932129410349 1104 52-53 0.11056540484983657 1104 54-55 0.0784240039015387 1104 56-57 0.05211846890873062 1104 58-59 0.048381350585152916 1104 60-61 0.0759975266973214 1104 62-63 0.09716189219172833 1104 64-65 0.14594643576442223 1104 66-67 0.06440857778346043 1104 68-69 0.2507756625419617 1104 70-71 0.11637716468634807 1104 72-73 -0.05190428693565252 1104 74-75 0.07436017928824157 1104 76-77 0.06614957303495572 1104 78-79 0.19330594860147698 1104 80-81 -0.02002901652610234 1104 82-83 -0.21093359923360566 1104 84-85 -0.23357843837688108 1104 86-87 -0.15184657250970446 1104 88-89 -0.06179703790550661 1104 90-91 -0.16957981978246295 1104 92-93 -0.14714266236863693 1104 94-95 -0.07744734989844204 1104 96-97 -0.18539796486582105 1104 98-99 -0.19547223235813505 1104 100-101 -0.1666198810879429 1105 1 0.08663511763179343 1105 2 0.14596297801761438 1105 3 0.2879731161058814 1105 4 -0.028904322956307738 1105 5 0.0548351311942028 1105 6 -0.06334950813668883 1105 7 0.025775953388340156 1105 8 0.17142886050950779 1105 9 0.17529633520852173 1105 10-11 0.3035531246463421 1105 12-13 0.2993764261389913 1105 14-15 0.4045551692734648 1105 16-17 0.3777846403237959 1105 18-19 0.5253944993860031 1105 20-21 0.45370671426549336 1105 22-23 0.46490087083964937 1105 24-25 0.32909876272012184 1105 26-27 0.4145079048225355 1105 28-29 0.39655660036179796 1105 30-31 0.39097209021122126 1105 32-33 0.33375058867913054 1105 34-35 0.39557838329247375 1105 36-37 0.3390380239416473 1105 38-39 0.2505989511768263 1105 40-41 0.30263349771411896 1105 42-43 0.44195207675921466 1105 44-45 0.3898913330165996 1105 46-47 0.48317953318631623 1105 48-49 0.40163764055358087 1105 50-51 0.5522716628611093 1105 52-53 0.6167406591250142 1105 54-55 0.4587730967073256 1105 56-57 0.355875780484304 1105 58-59 0.5515369707185371 1105 60-61 0.5465854332581799 1105 62-63 0.5688637661904536 1105 64-65 0.49497590560534377 1105 66-67 0.6281655167946738 1105 68-69 0.3792358540400471 1105 70-71 0.5359312051468379 1105 72-73 0.5761293183696665 1105 74-75 0.5838810615206427 1105 76-77 0.5161048653117035 1105 78-79 0.629893631623716 1105 80-81 0.7408964333453625 1105 82-83 0.5228989703427871 1105 84-85 0.631907199267026 1105 86-87 0.5784168304931612 1105 88-89 0.5075856229491578 1105 90-91 0.39953459473264985 1105 92-93 0.24665414102915406 1105 94-95 0.4497461227739663 1105 96-97 0.5406703634379042 1105 98-99 0.46509183008365795 1105 100-101 0.40309808769116273 1106 1 0.19294372803247484 1106 2 0.27401071885307715 1106 3 0.40920073758925923 1106 4 0.40477001634462795 1106 5 0.20743530169718127 1106 6 0.2839650698684224 1106 7 0.331317300361917 1106 8 0.3218124922230601 1106 9 0.37870639488456703 1106 10-11 0.30917972311181785 1106 12-13 0.2527012343231334 1106 14-15 0.41546736023680353 1106 16-17 0.4276567630859489 1106 18-19 0.3991370398804577 1106 20-21 0.46103834256899034 1106 22-23 0.4047559433034138 1106 24-25 0.2806759153202876 1106 26-27 0.37418394915330566 1106 28-29 0.41411840769342945 1106 30-31 0.29404114391965663 1106 32-33 0.23933456139865683 1106 34-35 0.28368580017226463 1106 36-37 0.32301074346424485 1106 38-39 0.430564850580069 1106 40-41 0.24167868100483503 1106 42-43 0.2896502864778867 1106 44-45 0.5138896279867566 1106 46-47 0.3356092007055054 1106 48-49 0.313487597927832 1106 50-51 0.4008650132447329 1106 52-53 0.29129308879252847 1106 54-55 0.27774155365531783 1106 56-57 0.46567970205292397 1106 58-59 0.3385361182036135 1106 60-61 0.42753172481828017 1106 62-63 0.49119965706854885 1106 64-65 0.4004320010870188 1106 66-67 0.39764548269406674 1106 68-69 0.36495622914660686 1106 70-71 0.5934759621800865 1106 72-73 0.5992324726748635 1106 74-75 0.5311103880338592 1106 76-77 0.4457723844932886 1106 78-79 0.3109678004216718 1106 80-81 0.5004872261842408 1106 82-83 0.20819308798984082 1106 84-85 0.2278151276557665 1106 86-87 0.34717215871140894 1106 88-89 0.06457624528504624 1106 90-91 0.12711345236265714 1106 92-93 0.10496616148950721 1106 94-95 0.3061399846665438 1106 96-97 0.29071298918385224 1106 98-99 0.025535137841536937 1106 100-101 0.09116287882500629 1107 1 -0.008999699690086516 1107 2 -0.03532661670598003 1107 3 0.08732224393718013 1107 4 -0.02620537303007353 1107 5 0.0848707122060901 1107 6 0.02706965353995372 1107 7 0.21360898298132724 1107 8 0.29798444499271426 1107 9 0.204464086662135 1107 10-11 0.18564112256355259 1107 12-13 0.030774499557374213 1107 14-15 0.1144397479422139 1107 16-17 0.1060975796625172 1107 18-19 0.19024306312808648 1107 20-21 0.007316885227915293 1107 22-23 0.057893147156576674 1107 24-25 0.049957046153252804 1107 26-27 0.14087690595071223 1107 28-29 0.28790433966580053 1107 30-31 0.13533553723022607 1107 32-33 -0.19697491683158574 1107 34-35 -0.1044476515942776 1107 36-37 0.032004265371831764 1107 38-39 0.07965822393565958 1107 40-41 -0.0684243865174139 1107 42-43 -0.11649624399058212 1107 44-45 0.2274032656730256 1107 46-47 0.09005274328785617 1107 48-49 0.16375254118709393 1107 50-51 -0.0379392197215509 1107 52-53 -0.10627418076043682 1107 54-55 -0.037929150971230285 1107 56-57 0.12983655458826604 1107 58-59 0.11300967747892088 1107 60-61 0.07308894796788934 1107 62-63 0.24516768010176548 1107 64-65 0.21104810902458837 1107 66-67 0.1718043261325164 1107 68-69 -0.03845051235759911 1107 70-71 0.09383279327492744 1107 72-73 -0.14224263337901277 1107 74-75 0.03564275552393781 1107 76-77 0.017888255053954083 1107 78-79 -0.012634355879534098 1107 80-81 -0.0019179379121183615 1107 82-83 0.10123794456824697 1107 84-85 0.055390667113933034 1107 86-87 0.30436927343337317 1107 88-89 -0.1278261621762411 1107 90-91 -0.13026667229851085 1107 92-93 -0.0454963406162463 1107 94-95 -0.057231226874570496 1107 96-97 0.09489235423890108 1107 98-99 0.09949259377366815 1107 100-101 0.02811327470843139 1108 1 0.20295593449608873 1108 2 0.11989249053408457 1108 3 0.16796323468295782 1108 4 0.11187301564711305 1108 5 -0.01851796498498004 1108 6 0.06392321913603638 1108 7 0.01225487301943673 1108 8 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48-49 -0.33516429181356955 2316 50-51 -0.6165979023562826 2316 52-53 -0.5518390510199183 2316 54-55 -0.38277279701248546 2316 56-57 -0.780327118066424 2316 58-59 -0.8480282466727758 2316 60-61 -0.41351118509930984 2316 62-63 -0.10034396327814221 2316 64-65 -0.19723665478112906 2316 66-67 -0.13288762330194714 2316 68-69 -0.042368010694261216 2316 70-71 -0.09114125862127764 2316 72-73 0.005443328031503825 2316 74-75 0.07469265572353834 2316 76-77 -0.25859078686220727 2316 78-79 -0.160802020550193 2316 80-81 0.011785322234253215 2316 82-83 -0.03777735815963723 2316 84-85 -0.06735850121607001 2316 86-87 -0.06912906322664014 2316 88-89 -0.05659795193006545 2316 90-91 -0.005045115412279699 2316 92-93 -0.014978709212392971 2316 94-95 -0.02845677577676753 2316 96-97 0.07340466295480752 2316 98-99 -0.01608691387769312 2316 100-101 0.045465237449615614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 6.0 7 68.0 8 178.0 9 157.0 10 236.0 11 374.0 12 571.0 13 984.0 14 1167.0 15 1336.0 16 1610.0 17 1922.0 18 2314.0 19 2810.0 20 3337.0 21 3939.0 22 4529.0 23 4731.0 24 4841.0 25 5121.0 26 5414.0 27 6189.0 28 7130.0 29 8343.0 30 9814.0 31 12264.0 32 15319.0 33 20099.0 34 27936.0 35 41521.0 36 70696.0 37 146637.0 38 286769.0 39 117230.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61735676013683 21.567944678085805 14.81945585404431 21.995242707733052 2 13.793805306479282 21.132853259503488 43.47529148361451 21.598049950402725 3 18.267586206896553 27.68760153256705 30.915800766283525 23.129011494252875 4 11.498963991025342 18.233757953974226 42.88979071392666 27.37748734107377 5 11.92656838512241 39.38005139673572 36.74327134337727 11.950108874764595 6 27.089594053955302 40.32989169949522 20.145880154634437 12.434634091915042 7 25.26940190264136 33.91319060866623 24.045644756088443 16.771762732603975 8 23.70099815229639 38.06821177356251 21.524091872678877 16.706698201462224 9 24.823437668592504 18.27898319715146 22.04785329632064 34.849725837935395 10-11 23.440771223198002 28.3105736662222 30.024368936502828 18.22428617407697 12-13 24.18579046035652 24.857069642609737 30.395337460320963 20.561802436712778 14-15 20.961669081965688 27.29306281984321 27.74203870853839 24.00322938965271 16-17 20.917899449998405 30.659830267007017 27.94218975668008 20.4800805263145 18-19 20.53515490422776 29.792872529679403 29.57757962220754 20.0943929438853 20-21 21.377379090668892 28.55779304493567 29.846197041152056 20.21863082324338 22-23 21.148705021814386 28.439558795853277 29.415367111250607 20.996369071081734 24-25 20.813509615001475 28.79547194702477 29.51473499205825 20.876283445915504 26-27 20.826497958583147 29.034465001655203 29.42208898860975 20.7169480511519 28-29 20.940336250363988 28.808226946006837 29.399132553755614 20.85230424987356 30-31 21.363309482631248 28.482103390371886 29.374619509674034 20.779967617322832 32-33 21.180082378089562 28.68906776912802 29.254581890429414 20.876267962353005 34-35 21.16212664013516 28.916462530988163 29.23964670428158 20.6817641245951 36-37 21.3630877103503 28.752619896068516 29.120677363055837 20.763615030525354 38-39 21.230210909937995 28.679092606045003 29.18330252901889 20.907393954998117 40-41 21.444238469705777 28.56471162310546 29.150820098230334 20.84022980895843 42-43 21.173186960311806 28.573328935930743 29.1026724029067 21.15081170085075 44-45 21.393024761288235 28.802100957789577 28.773288803228926 21.031585477693262 46-47 21.210345277847427 28.72593007637762 28.866498246422807 21.197226399352147 48-49 21.362959239346996 28.558388455337386 29.014853821962568 21.063798483353054 50-51 21.4032530173661 28.725239452092417 28.63303874881684 21.23846878172464 52-53 21.34468401268216 28.589232425328028 28.864478430276325 21.20160513171349 54-55 21.344745314687717 28.730472246130006 28.752050552085862 21.17273188709642 56-57 21.315933372076202 28.913213524693678 28.672235340851408 21.098617762378712 58-59 21.46587807766719 28.57188395775556 28.80133736455322 21.16090060002403 60-61 21.416959077233173 28.899849687482376 28.823957804603534 20.859233430680916 62-63 21.205465243303617 28.794412151633036 28.914503811178392 21.08561879388495 64-65 21.461640130551313 28.560245114747346 28.721717764934073 21.256396989767268 66-67 21.3449270671262 28.61963946136219 28.859397778112907 21.176035693398706 68-69 21.382902481624917 28.44074943490754 29.02469363620368 21.151654447263862 70-71 21.565858027449963 28.3381118510304 28.795549940260866 21.30048018125877 72-73 21.289634811692498 28.59309445167808 28.847497774737196 21.269772961892222 74-75 21.382139538077126 28.37786311016951 28.705215819840763 21.534781531912596 76-77 21.244455233597424 28.59989897429484 28.792264667730873 21.363381124376865 78-79 21.25046283014195 28.37105858755275 28.714901536718674 21.663577045586624 80-81 21.32124288797332 28.69886698057681 28.497400431626446 21.482489699823425 82-83 21.39907780195365 28.687336115499757 28.542158281849765 21.37142780069683 84-85 21.444184274146327 28.57742845878835 28.718079832837006 21.260307434228316 86-87 21.118860881806185 28.490217140727008 28.95943387434581 21.431488103120998 88-89 21.181211032843844 28.66750336139142 28.783504320299986 21.367781285464748 90-91 21.396803753192753 28.606077847358552 28.544460644080637 21.452657755368055 92-93 21.346234961179857 28.646775941811477 28.795324907179886 21.21166418982878 94-95 21.33167694855662 28.511994584455696 28.817722663059577 21.33860580392811 96-97 21.182515466243405 28.478732267693687 29.044657915694184 21.29409435036873 98-99 21.293748482552108 28.289520474204 28.725809734719455 21.690921308524437 100-101 21.164004944133254 28.25035069992839 28.953528580817938 21.632115775120415 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 3.5 2 9.0 3 26.0 4 25.5 5 17.5 6 33.0 7 50.0 8 90.5 9 136.0 10 142.5 11 137.5 12 154.5 13 180.5 14 205.0 15 223.5 16 229.0 17 266.5 18 323.0 19 346.0 20 386.5 21 454.5 22 540.5 23 686.5 24 860.0 25 1055.0 26 1385.0 27 1812.0 28 2365.0 29 3129.5 30 4337.5 31 6380.5 32 9732.5 33 15531.0 34 22980.5 35 31035.5 36 40891.0 37 49687.5 38 54645.5 39 56261.5 40 53675.0 41 48716.5 42 43209.5 43 38361.0 44 34431.5 45 30187.0 46 26711.0 47 24455.0 48 22927.5 49 21619.5 50 20162.5 51 18804.0 52 17413.0 53 16136.0 54 14849.0 55 13436.5 56 11946.0 57 10323.0 58 8905.5 59 7472.0 60 6096.5 61 4826.5 62 3737.0 63 2793.5 64 2034.0 65 1522.0 66 1080.5 67 776.0 68 581.0 69 445.0 70 336.0 71 246.0 72 181.5 73 132.0 74 103.5 75 72.5 76 45.5 77 33.0 78 26.0 79 17.0 80 10.0 81 8.0 82 6.5 83 7.0 84 5.5 85 2.5 86 2.0 87 1.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005394576488964413 2 0.007969260722333792 3 0.0011034361000154481 4 4.904160444513103E-4 5 0.0022068722000308963 6 0.00404593236672331 7 0.0038007243444976545 8 0.003310308300046344 9 0.007111032644543999 10-11 0.005701086516746482 12-13 0.003310308300046344 14-15 0.0 16-17 0.0 18-19 1.2260401111282757E-4 20-21 0.0015325501389103447 22-23 3.065100277820689E-4 24-25 9.195300833462068E-4 26-27 0.0029424962667078617 28-29 0.0026359862389257927 30-31 0.009133998827905653 32-33 5.517180500077241E-4 34-35 0.0 36-37 6.130200555641379E-5 38-39 1.8390601666924136E-4 40-41 0.0 42-43 1.2260401111282757E-4 44-45 7.356240666769654E-4 46-47 0.0019003621722488272 48-49 0.002329476211143724 50-51 0.0026972882444822066 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 8.58228077789793E-4 64-65 0.001961664177805241 66-67 0.002574684233369379 68-69 0.00729493866121324 70-71 5.517180500077241E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.013976857266862344 82-83 0.010114830916808273 84-85 0.017471071583577927 86-87 0.015448105400216273 88-89 0.015141595372434206 90-91 0.01409946127797517 92-93 0.00956311286680055 94-95 0.02494991626146041 96-97 0.00827577075011586 98-99 0.014712481333539308 100-101 0.014957689355764964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 815634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.07796180875461 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1297356626149 42.692004089987016 2 13.113073613785692 14.707088826185236 3 4.140147772469978 6.965131460015156 4 1.9080818184453847 4.280053573710373 5 1.116782567872642 3.131344509492245 6 0.728341720711817 2.4506351518679934 7 0.5015616983418325 1.968859043504317 8 0.36402409019619913 1.6330983221991275 9 0.2924575921851419 1.4760383116714844 >10 1.6183948789510842 16.326111687461655 >50 0.07106700030216724 2.666540284679345 >100 0.015669492876613542 1.442451071218442 >500 6.620912465227128E-4 0.26064366800760813 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 847 0.10384559741256495 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.2260401111282757E-4 0.0 0.0 0.0 0.0 7 1.2260401111282757E-4 0.0 0.0 0.0 0.0 8 1.2260401111282757E-4 0.0 0.0 0.0 0.0 9 1.2260401111282757E-4 0.0 0.0 0.0 0.0 10-11 1.2260401111282757E-4 0.0 0.0 1.2260401111282757E-4 0.0 12-13 1.2260401111282757E-4 0.0 0.0 2.4520802222565514E-4 0.0 14-15 1.2260401111282757E-4 0.0 0.0 2.4520802222565514E-4 0.0 16-17 1.2260401111282757E-4 0.0 0.0 3.678120333384827E-4 0.0 18-19 1.2260401111282757E-4 0.0 0.0 4.904160444513103E-4 1.2260401111282757E-4 20-21 1.2260401111282757E-4 0.0 0.0 7.356240666769654E-4 2.4520802222565514E-4 22-23 1.2260401111282757E-4 0.0 0.0 9.195300833462068E-4 2.4520802222565514E-4 24-25 1.2260401111282757E-4 0.0 0.0 0.0011647381055718618 2.4520802222565514E-4 26-27 1.2260401111282757E-4 0.0 0.0 0.001409946127797517 2.4520802222565514E-4 28-29 1.2260401111282757E-4 0.0 0.0 0.0015938521444667584 2.4520802222565514E-4 30-31 1.2260401111282757E-4 0.0 0.0 0.0021455701944744824 2.4520802222565514E-4 32-33 3.678120333384827E-4 0.0 0.0 0.00275859025003862 3.678120333384827E-4 34-35 6.130200555641379E-4 0.0 0.0 0.0038620263500540684 3.678120333384827E-4 36-37 6.130200555641379E-4 0.0 0.0 0.006007596544528551 3.678120333384827E-4 38-39 6.130200555641379E-4 0.0 0.0 0.01054394495570317 3.678120333384827E-4 40-41 6.130200555641379E-4 0.0 0.0 0.015570709411329101 3.678120333384827E-4 42-43 6.130200555641379E-4 0.0 0.0 0.020474869855842202 3.678120333384827E-4 44-45 6.130200555641379E-4 0.0 0.0 0.02654376840592717 3.678120333384827E-4 46-47 6.130200555641379E-4 0.0 0.0 0.037700733417194474 3.678120333384827E-4 48-49 6.130200555641379E-4 0.0 0.0 0.04781556433400275 4.904160444513103E-4 50-51 6.130200555641379E-4 0.0 0.0 0.059524247395277785 4.904160444513103E-4 52-53 6.130200555641379E-4 0.0 0.0 0.07552407084550178 4.904160444513103E-4 54-55 6.130200555641379E-4 0.0 0.0 0.09409857852909516 4.904160444513103E-4 56-57 6.130200555641379E-4 0.0 0.0 0.11579948849606564 4.904160444513103E-4 58-59 6.130200555641379E-4 0.0 0.0 0.14111721679086453 4.904160444513103E-4 60-61 6.743220611205516E-4 0.0 0.0 0.17060348146349957 4.904160444513103E-4 62-63 7.356240666769654E-4 0.0 0.0 0.20229661833616547 6.130200555641379E-4 64-65 7.356240666769654E-4 0.0 0.0 0.23785178155888548 6.130200555641379E-4 66-67 7.356240666769654E-4 0.0 0.0 0.2748781929149594 6.130200555641379E-4 68-69 7.356240666769654E-4 0.0 0.0 0.3231841732934134 6.130200555641379E-4 70-71 7.356240666769654E-4 0.0 0.0 0.3830149307164733 6.130200555641379E-4 72-73 8.58228077789793E-4 0.0 0.0 0.4405162119283894 6.130200555641379E-4 74-75 8.58228077789793E-4 0.0 0.0 0.5062319618848651 6.130200555641379E-4 76-77 8.58228077789793E-4 0.0 0.0 0.588437951336016 6.130200555641379E-4 78-79 8.58228077789793E-4 0.0 0.0 0.6830882479151188 6.130200555641379E-4 80-81 8.58228077789793E-4 0.0 0.0 0.7876081673888042 6.130200555641379E-4 82-83 8.58228077789793E-4 0.0 0.0 0.8977678713736799 6.130200555641379E-4 84-85 8.58228077789793E-4 0.0 0.0 1.0247243248810127 6.130200555641379E-4 86-87 8.58228077789793E-4 0.0 0.0 1.1583013949884386 6.130200555641379E-4 88-89 8.58228077789793E-4 0.0 0.0 1.3148054151739628 6.130200555641379E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 980 0.0 40.72091 1 GTATCAA 1670 0.0 34.13729 1 ATCAACG 1640 0.0 32.43833 3 TCAACGC 1655 0.0 32.144333 4 CAACGCA 1660 0.0 32.04751 5 AACGCAG 1695 0.0 31.385763 6 TACGCTG 65 0.005843699 29.230146 5 TATCAAC 1870 0.0 29.215982 2 CATTGCG 70 0.008391037 27.142279 9 ACGCAGA 1940 0.0 26.932415 7 CGCAGAG 1975 0.0 26.214632 8 GGGGCGT 95 0.0012264014 24.999468 6 GTACATG 2445 0.0 24.288204 1 ACATGGG 2270 0.0 23.435623 3 TAGCCCT 840 0.0 23.18403 4 TACATGG 2450 0.0 23.075182 2 ATAGACC 295 5.456968E-12 22.541893 3 TTAGGCG 110 0.0028770124 21.59045 9 GTAGCCC 515 0.0 21.213142 3 GTTACAC 385 0.0 20.97358 3 >>END_MODULE