FastQCFastQC Report
Fri 10 Feb 2017
SRR2568705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815634
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.11708683061275031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.11218267016823721No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC8700.10666548966815999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9200.045.5188261
CCGTTAT853.6405072E-739.115539
TCAACGC16100.034.5273444
GTATCAA17100.034.2298661
ATCAACG16250.034.2086333
CAACGCA16600.033.4873665
ACCGTTA1001.2991295E-633.2481968
CCGATCA600.003951333531.664959
AACGCAG17850.031.4084876
CTTCGCG404.4958864E-429.68588822-23
TATCAAC19700.028.2576392
CGCAGAG20150.027.1077128
ACGCAGA20750.027.0188677
TACCGTT1257.372988E-626.6067127
TAATACT7700.025.2985924
GTACATG21600.024.0142441
ACATGGG19750.023.8161353
TCGTCCG500.001654463923.74871310-11
ATACCGT1652.423123E-623.0361166
GTTACAC3950.022.8538673
Produced by FastQC (version 0.11.5)