##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568705_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 815634 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69194516167791 34.0 31.0 34.0 31.0 34.0 2 32.80995397445423 34.0 31.0 34.0 31.0 34.0 3 32.88311791808581 34.0 31.0 34.0 31.0 34.0 4 36.254241485764446 37.0 37.0 37.0 35.0 37.0 5 36.30922202850788 37.0 37.0 37.0 35.0 37.0 6 36.330628688848186 37.0 37.0 37.0 35.0 37.0 7 36.28335993840374 37.0 37.0 37.0 35.0 37.0 8 36.2827162673454 37.0 37.0 37.0 35.0 37.0 9 38.10644602848827 39.0 39.0 39.0 37.0 39.0 10-11 38.044714908892956 39.0 39.0 39.0 35.0 39.0 12-13 38.04027603067062 39.0 39.0 39.0 35.0 39.0 14-15 39.51112018380793 41.0 39.0 41.0 37.0 41.0 16-17 39.50509174458152 41.0 39.0 41.0 37.0 41.0 18-19 39.56451913480801 41.0 39.5 41.0 37.0 41.0 20-21 39.53558826630572 41.0 40.0 41.0 37.0 41.0 22-23 39.51452918833692 41.0 39.5 41.0 37.0 41.0 24-25 39.505014504054515 41.0 39.0 41.0 37.0 41.0 26-27 39.41600031386627 41.0 39.0 41.0 36.0 41.0 28-29 39.3242011735656 41.0 39.0 41.0 36.0 41.0 30-31 39.27643219875581 41.0 39.0 41.0 36.0 41.0 32-33 39.15826522680516 41.0 39.0 41.0 36.0 41.0 34-35 39.073834587572364 41.0 39.0 41.0 35.0 41.0 36-37 39.006465522526035 41.0 39.0 41.0 35.0 41.0 38-39 38.889261605082666 40.0 38.5 41.0 35.0 41.0 40-41 38.83229671641938 40.0 38.0 41.0 35.0 41.0 42-43 38.73295681641521 40.0 38.0 41.0 35.0 41.0 44-45 38.641349551391926 40.0 38.0 41.0 35.0 41.0 46-47 38.47925601433977 40.0 38.0 41.0 34.0 41.0 48-49 38.362220677411685 40.0 38.0 41.0 34.0 41.0 50-51 38.38915310053284 40.0 38.0 41.0 34.0 41.0 52-53 38.481446334998296 40.0 38.0 41.0 34.0 41.0 54-55 38.3888625290265 40.0 38.0 41.0 34.0 41.0 56-57 38.288129234436035 40.0 38.0 41.0 34.0 41.0 58-59 38.13779035694932 40.0 38.0 41.0 34.0 41.0 60-61 38.103575868588116 40.0 37.5 41.0 34.0 41.0 62-63 37.97788346243536 40.0 37.0 41.0 33.5 41.0 64-65 37.8065633605269 40.0 37.0 41.0 33.0 41.0 66-67 37.61597174713168 39.5 36.5 41.0 33.0 41.0 68-69 37.37035974468941 39.0 36.0 41.0 33.0 41.0 70-71 37.04118943055341 39.0 35.5 41.0 32.0 41.0 72-73 36.669050701662755 38.0 35.0 40.0 32.0 41.0 74-75 36.28896355473165 37.0 35.0 39.5 32.0 41.0 76-77 34.41332448132373 35.5 33.0 38.5 29.0 39.0 78-79 35.34196465571567 36.0 35.0 39.0 31.0 39.5 80-81 35.16037217673613 36.0 35.0 37.5 31.0 39.0 82-83 34.85274645245294 35.5 35.0 37.0 31.0 39.0 84-85 34.48036128949995 35.0 35.0 37.0 31.0 38.0 86-87 34.201654173317934 35.0 34.0 36.0 31.0 37.0 88-89 33.982619655384646 35.0 34.0 36.0 31.0 37.0 90-91 33.80579279431706 35.0 34.0 36.0 31.0 36.5 92-93 33.664592819818694 35.0 34.0 35.0 31.0 36.0 94-95 33.54247064246954 35.0 34.0 35.0 31.0 36.0 96-97 33.43336349392007 35.0 34.0 35.0 30.0 36.0 98-99 33.357001424658606 35.0 34.0 35.0 30.0 36.0 100-101 32.33319601684089 34.5 32.5 35.0 27.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2876900235031101 1101 2 0.25968357758043936 1101 3 0.1579200684277211 1101 4 0.16984060694234415 1101 5 0.17205311291955638 1101 6 0.1568829649840282 1101 7 0.19987873898004693 1101 8 0.23500381611173538 1101 9 0.25856148393421563 1101 10-11 0.1963630931154725 1101 12-13 0.2559719908269855 1101 14-15 0.2577996137151999 1101 16-17 0.29580961604241196 1101 18-19 0.33393365453671464 1101 20-21 0.4118642764507854 1101 22-23 0.2300172703609178 1101 24-25 0.2866225807529901 1101 26-27 0.3374286907613353 1101 28-29 0.26888771718365945 1101 30-31 0.01541146935669957 1101 32-33 0.18070679946588797 1101 34-35 0.29019513858079904 1101 36-37 0.11049086762616156 1101 38-39 0.1996617794140505 1101 40-41 0.16792909322403204 1101 42-43 0.15161256325343686 1101 44-45 0.1472024632574076 1101 46-47 0.19173633281416613 1101 48-49 0.1852884557296548 1101 50-51 0.17372219249637055 1101 52-53 0.1971914980945968 1101 54-55 0.20061293765242283 1101 56-57 0.06573285223690561 1101 58-59 0.24444417608651037 1101 60-61 0.2078454757456356 1101 62-63 0.21555824829641068 1101 64-65 0.05251999152539355 1101 66-67 0.07819764057295231 1101 68-69 0.22988637006414336 1101 70-71 0.20388535002931718 1101 72-73 0.0996722341557259 1101 74-75 -0.031987481183513466 1101 76-77 -0.04054603263288925 1101 78-79 0.06933556790093576 1101 80-81 0.1417796576209298 1101 82-83 0.016779337572437214 1101 84-85 0.1261627778129366 1101 86-87 0.20363425754182884 1101 88-89 0.3128919566378485 1101 90-91 0.2686639692752024 1101 92-93 0.16917049005081708 1101 94-95 0.20842918757136175 1101 96-97 0.2546580725849452 1101 98-99 0.12307826992680049 1101 100-101 0.14261073201509333 1102 1 0.33565114947629837 1102 2 0.3141175539524994 1102 3 0.2896903911655926 1102 4 0.23129760753497663 1102 5 0.14879811473808502 1102 6 0.15254803836207742 1102 7 0.17136939617928704 1102 8 0.23664879891418877 1102 9 0.2223367840835735 1102 10-11 0.30457286811653006 1102 12-13 0.27365878683988143 1102 14-15 0.3607042730228329 1102 16-17 0.3509100781515002 1102 18-19 0.26797095189089504 1102 20-21 0.35446988490056697 1102 22-23 0.2741031787108241 1102 24-25 0.33034755292121076 1102 26-27 0.3144792421351994 1102 28-29 0.35849773994755907 1102 30-31 0.4306680848276585 1102 32-33 0.5009624272086839 1102 34-35 0.48794939550458594 1102 36-37 0.4430220179879285 1102 38-39 0.480013189096006 1102 40-41 0.4665903984652644 1102 42-43 0.4294614205350342 1102 44-45 0.5366286024218425 1102 46-47 0.3668125355809124 1102 48-49 0.4686021268106302 1102 50-51 0.4257830365516213 1102 52-53 0.602131431298325 1102 54-55 0.6247424906638841 1102 56-57 0.6660001850200672 1102 58-59 0.7345558170630113 1102 60-61 0.6175514281463634 1102 62-63 0.8212562397927101 1102 64-65 0.6860331467722673 1102 66-67 0.6669297853092857 1102 68-69 0.5464347862835623 1102 70-71 0.6704918298953615 1102 72-73 0.6714145312606661 1102 74-75 0.5045279984554369 1102 76-77 0.2994466194080161 1102 78-79 0.500487222022528 1102 80-81 0.45344112717582163 1102 82-83 0.4589427761219014 1102 84-85 0.374316095379541 1102 86-87 0.3800360468991073 1102 88-89 0.3299316522329434 1102 90-91 0.401884498512878 1102 92-93 0.33621378577663563 1102 94-95 0.5387504115419617 1102 96-97 0.42050686340257926 1102 98-99 0.2920665593477665 1102 100-101 0.43830700652598154 1103 1 0.2952186546320874 1103 2 0.2105879703452942 1103 3 0.2541493726673636 1103 4 0.15773418527519567 1103 5 0.13795096598364864 1103 6 0.07392446815817522 1103 7 0.12095571604324107 1103 8 0.07350510810140065 1103 9 0.10867473241094672 1103 10-11 0.17933594538202868 1103 12-13 0.15246128804840708 1103 14-15 0.1575428108313517 1103 16-17 0.2780248200491755 1103 18-19 0.15250480967117142 1103 20-21 0.3208268566019967 1103 22-23 0.1846142014867027 1103 24-25 0.2186495689815331 1103 26-27 0.2642637945990245 1103 28-29 0.2795107433105599 1103 30-31 0.24608745110933228 1103 32-33 0.1809141558317222 1103 34-35 0.06279698245739951 1103 36-37 9.189787691710194E-4 1103 38-39 -0.008436284196498889 1103 40-41 0.15950723467297223 1103 42-43 0.19543653364932823 1103 44-45 0.16067265179218992 1103 46-47 0.26151972516431243 1103 48-49 0.3160186691472049 1103 50-51 0.23828977472113877 1103 52-53 0.24800975821518279 1103 54-55 0.16750769632151474 1103 56-57 0.18874302864932702 1103 58-59 0.3124012373682916 1103 60-61 0.3584101230803114 1103 62-63 0.18759703988730791 1103 64-65 0.08722028182430819 1103 66-67 0.13959107535139736 1103 68-69 0.2513238281941099 1103 70-71 0.2215106411257537 1103 72-73 0.1073045434676203 1103 74-75 0.13010139494498674 1103 76-77 0.06794919817069456 1103 78-79 0.050099277346809856 1103 80-81 0.2621101517670965 1103 82-83 0.05851379382024646 1103 84-85 0.1948695121096904 1103 86-87 0.19952700644921606 1103 88-89 0.2456367169019842 1103 90-91 0.18161417184641238 1103 92-93 0.13323403680170998 1103 94-95 0.053278774453680455 1103 96-97 0.19131867806553515 1103 98-99 0.1645256173603613 1103 100-101 0.14331255356101025 1104 1 0.22178116501117984 1104 2 -0.10078486000882236 1104 3 -0.05008928339768204 1104 4 -0.061754598660293425 1104 5 0.012060024525055724 1104 6 -0.004136285247831495 1104 7 0.08659011950271633 1104 8 0.11132486723998625 1104 9 0.09056362794245132 1104 10-11 0.02946016238315252 1104 12-13 0.11971815153591336 1104 14-15 0.16217437789192957 1104 16-17 0.18435556940318065 1104 18-19 0.015442641387025446 1104 20-21 0.04489240597990829 1104 22-23 0.09524272622714847 1104 24-25 0.06355932898365779 1104 26-27 -0.0770058094343753 1104 28-29 -0.08004433397632482 1104 30-31 -0.08211445797975614 1104 32-33 -0.09169068132442959 1104 34-35 -0.12913655148306447 1104 36-37 -0.09388283865176561 1104 38-39 -0.24210706264518222 1104 40-41 -0.35756097839571765 1104 42-43 -0.23071911623143393 1104 44-45 -0.36956787749179654 1104 46-47 -0.24973453441600668 1104 48-49 -0.1569259380414465 1104 50-51 -0.32179936924437413 1104 52-53 -0.28764616733433 1104 54-55 -0.07548101786645844 1104 56-57 -0.16563578670575652 1104 58-59 -0.27961067949267004 1104 60-61 -0.2948019160048716 1104 62-63 -0.3540206374540489 1104 64-65 -0.21200319585316407 1104 66-67 -0.10560963142341251 1104 68-69 -0.2579874649248026 1104 70-71 -0.27566458455726917 1104 72-73 -0.27942051183219263 1104 74-75 -0.17503567552657984 1104 76-77 -0.43495362693575146 1104 78-79 -0.20610160877874506 1104 80-81 -0.12335446631544755 1104 82-83 -0.13219749797013947 1104 84-85 -0.024772052094562014 1104 86-87 -0.22376567404738523 1104 88-89 -0.18188731277724202 1104 90-91 -0.14947229514307736 1104 92-93 -0.16547463779625105 1104 94-95 -0.4326372096837403 1104 96-97 -0.2684270424262891 1104 98-99 -0.24009133593537513 1104 100-101 -0.3220960008055087 1105 1 0.36597474945289576 1105 2 0.11523913313599365 1105 3 0.19422352163938683 1105 4 0.19832513011285613 1105 5 0.13477154794072987 1105 6 0.2802383654730818 1105 7 0.2506153048534898 1105 8 0.1635062315706719 1105 9 0.3360649132894977 1105 10-11 0.3455251376076305 1105 12-13 0.2690661639050447 1105 14-15 0.3210637635332958 1105 16-17 0.3560946998379677 1105 18-19 0.35659423782531263 1105 20-21 0.34505447147672896 1105 22-23 0.40657240842974574 1105 24-25 0.2967138314239506 1105 26-27 0.32319537532028875 1105 28-29 0.3801061820253082 1105 30-31 0.4383523651639365 1105 32-33 0.495867531856895 1105 34-35 0.44802897886229687 1105 36-37 0.3884147657593999 1105 38-39 0.42158281478114645 1105 40-41 0.5064064594791802 1105 42-43 0.3846119072028671 1105 44-45 0.539759271273148 1105 46-47 0.4359720338936697 1105 48-49 0.42829257095598194 1105 50-51 0.44008170821175696 1105 52-53 0.5799612243425827 1105 54-55 0.4688839461810872 1105 56-57 0.5428605435352907 1105 58-59 0.5193442774761436 1105 60-61 0.4912772166653028 1105 62-63 0.5650602462686081 1105 64-65 0.665065467758474 1105 66-67 0.5853724482308493 1105 68-69 0.5131034035227273 1105 70-71 0.5299872167914117 1105 72-73 0.5932667949000461 1105 74-75 0.47043394184982645 1105 76-77 0.5729149323578611 1105 78-79 0.6911283752009894 1105 80-81 0.6211259926838508 1105 82-83 0.5148052217496968 1105 84-85 0.5716867236589422 1105 86-87 0.6136658672513775 1105 88-89 0.5672129442921587 1105 90-91 0.44290503804122494 1105 92-93 0.41990407387618944 1105 94-95 0.6139966713192067 1105 96-97 0.521500680087776 1105 98-99 0.5871803752527356 1105 100-101 0.6626530770976942 1106 1 0.2091972558467532 1106 2 0.015580139103597901 1106 3 0.10530621558653053 1106 4 0.11102760240611786 1106 5 0.11635722569008777 1106 6 0.08228440127870584 1106 7 0.08070908149457523 1106 8 0.10144314546666067 1106 9 0.15703678029716883 1106 10-11 0.1637263311285153 1106 12-13 0.18066036680360043 1106 14-15 0.07212940718206085 1106 16-17 0.12169572285586838 1106 18-19 0.05049875615438282 1106 20-21 0.18034858912378127 1106 22-23 0.10205407935898592 1106 24-25 0.09292014003435156 1106 26-27 0.06411609143282249 1106 28-29 0.18556227750698184 1106 30-31 -0.008621206873570486 1106 32-33 -2.5352526160560274E-4 1106 34-35 0.07411593538404304 1106 36-37 0.11407546482163156 1106 38-39 0.20819833055266912 1106 40-41 0.18884465214755153 1106 42-43 0.23751045792749892 1106 44-45 0.03656234032686001 1106 46-47 0.07416470226537086 1106 48-49 0.2777810620045855 1106 50-51 0.3246085624317061 1106 52-53 0.3496969447176852 1106 54-55 0.35920790185030427 1106 56-57 0.21378978479701516 1106 58-59 0.27318059461169497 1106 60-61 0.285011232010568 1106 62-63 0.5223066230802047 1106 64-65 0.38825387355557694 1106 66-67 0.3364807176255695 1106 68-69 0.3523617155431893 1106 70-71 0.3722719567743624 1106 72-73 0.4305216968608292 1106 74-75 0.41872891201180096 1106 76-77 0.4464443441225683 1106 78-79 0.46678907426322525 1106 80-81 0.23873042576143177 1106 82-83 0.2832621697462798 1106 84-85 0.273178624767219 1106 86-87 0.22491906418232332 1106 88-89 0.3143996450594315 1106 90-91 0.2523679536422563 1106 92-93 0.29462700652751295 1106 94-95 0.21813136867640281 1106 96-97 0.31745123422247445 1106 98-99 0.42464840594327313 1106 100-101 0.37774259116419273 1107 1 0.1262975820485721 1107 2 -0.0011281323330720738 1107 3 0.04979064825571555 1107 4 0.07165672778707233 1107 5 0.09407901995322732 1107 6 0.11056900853478879 1107 7 0.14073940448032118 1107 8 0.07346023660408463 1107 9 0.0755858713733275 1107 10-11 0.03163986467523472 1107 12-13 0.013112853135289981 1107 14-15 0.14236464004115135 1107 16-17 0.18705018018162178 1107 18-19 0.20603882690802067 1107 20-21 0.07103728845147117 1107 22-23 0.1981197485321502 1107 24-25 0.1788972905925732 1107 26-27 0.2219066484262484 1107 28-29 0.0655092888551323 1107 30-31 0.03806164226191555 1107 32-33 0.14475237087450665 1107 34-35 0.01181445139549453 1107 36-37 0.06510920297193223 1107 38-39 0.04199850513955994 1107 40-41 0.10890146945921231 1107 42-43 0.015497961181864639 1107 44-45 0.014219047373124738 1107 46-47 0.08768599727125803 1107 48-49 0.15434047514759897 1107 50-51 0.09885546504591503 1107 52-53 0.10619571188923516 1107 54-55 0.2928794334673839 1107 56-57 0.2580215259183518 1107 58-59 0.27132292886900444 1107 60-61 0.33980624556402717 1107 62-63 0.3973001143585222 1107 64-65 0.39034968193880815 1107 66-67 0.1811374399864647 1107 68-69 0.1952174363266792 1107 70-71 0.1588034972739223 1107 72-73 0.12655673675546097 1107 74-75 0.2697136433164502 1107 76-77 0.006343726942603212 1107 78-79 0.2497155486801148 1107 80-81 0.24313415028169771 1107 82-83 0.2811855046621332 1107 84-85 0.18356731031173013 1107 86-87 0.20278328459060901 1107 88-89 0.11152432153767933 1107 90-91 0.16748197113157204 1107 92-93 0.11613335444548767 1107 94-95 0.07211087768907731 1107 96-97 0.1231712519874506 1107 98-99 0.20051023726783512 1107 100-101 0.2057798669961599 1108 1 0.0854905597295712 1108 2 0.09629973919659562 1108 3 -9.510501656180281E-4 1108 4 0.017165709822997144 1108 5 0.055571943197648466 1108 6 0.0183806579631991 1108 7 0.037983552547821375 1108 8 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2316 62-63 -0.2791523141178587 2316 64-65 -0.3373016819999819 2316 66-67 -0.47339083679330685 2316 68-69 -0.3622782389893544 2316 70-71 -0.14540408755641465 2316 72-73 -0.13927426790188946 2316 74-75 -0.06628103399559393 2316 76-77 -0.1722058405889939 2316 78-79 -0.23441751851881776 2316 80-81 -0.0049802875093831744 2316 82-83 -0.19472134829861432 2316 84-85 -0.39163694783863434 2316 86-87 -0.38034379458436973 2316 88-89 -0.2523426112633942 2316 90-91 -0.33081476872205684 2316 92-93 -0.23693650583395254 2316 94-95 -0.09882458471944489 2316 96-97 -0.3147795131489417 2316 98-99 -0.3791384653269745 2316 100-101 -0.22842904850616463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 0.0 11 0.0 12 0.0 13 3.0 14 15.0 15 36.0 16 114.0 17 268.0 18 499.0 19 814.0 20 1309.0 21 1979.0 22 2500.0 23 2729.0 24 2786.0 25 2991.0 26 3412.0 27 4377.0 28 5615.0 29 7049.0 30 8982.0 31 11385.0 32 14331.0 33 18562.0 34 25925.0 35 38378.0 36 66487.0 37 147151.0 38 309910.0 39 137984.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.082096506802955 22.278205040170295 15.118916824437663 21.520781628589088 2 13.824437983330654 21.188553110068987 43.651178403243044 21.33583050335731 3 17.803941473749255 28.29112077230719 31.313677458271727 22.591260295671834 4 11.565604180306362 18.5422628286707 42.9491659249124 26.942967066110533 5 11.80786970626531 39.683240276888895 36.996128165329054 11.512761851516734 6 26.856382685245922 41.102739684038234 19.81253180154609 12.228345829169758 7 24.66500528584008 34.052828966894204 24.600128037518427 16.682037709747284 8 23.375312946738365 38.63558900192979 21.614351535124822 16.374746516207026 9 24.314582275873736 18.188305048587967 22.23031408695567 35.266798588582624 10-11 23.15419661269638 28.486245055993255 30.173766664950207 18.185791666360156 12-13 23.83526189442814 25.06577705196203 30.68766137752963 20.4112996760802 14-15 20.772613696829705 27.325491580782558 27.93973767645782 23.962157045929917 16-17 20.58006409737701 30.840671183398438 28.232516054995255 20.3467486642293 18-19 20.304756790423156 29.890735305296246 29.842061512884456 19.962446391396142 20-21 21.30520552110383 28.764678765230485 29.859103470429137 20.07101224323655 22-23 20.749870652768276 28.900156197510157 29.675871775821015 20.674101373900548 24-25 20.54585757827653 28.877045341415393 29.892267855435158 20.68482922487292 26-27 20.661166650728145 29.07339566521258 29.554800314847103 20.710637369212172 28-29 20.801953849860322 28.8595615240176 29.562400650792863 20.77608397532921 30-31 21.22036354541375 28.649430994784424 29.592317142247627 20.5378883175542 32-33 21.07231920199501 28.48888104223218 29.59053938408649 20.84826037168632 34-35 21.02382931559989 28.870976442865302 29.45683970996795 20.648354531566852 36-37 21.156548157629526 28.690809848535004 29.298803139643514 20.853838854191952 38-39 20.9737455770603 28.927435589982764 29.302052145938006 20.796766687018934 40-41 21.206325386141334 28.712081644463076 29.51152109892427 20.570071870471317 42-43 20.97423599310475 28.446827866420477 29.428947297439784 21.14998884303499 44-45 21.15029535306277 28.73274042033559 29.127464034113338 20.989500192488297 46-47 21.346093958809956 28.540068216871784 29.001733620717136 21.112104203601127 48-49 21.254692668525344 28.642319962139883 29.13273600659119 20.970251362743582 50-51 21.231152698391682 28.738625412869006 28.96433939732772 21.06588249141159 52-53 21.265236613481047 28.641277828045425 29.08216185200715 21.01132370646638 54-55 21.179291201690955 28.925903039843853 28.82886196504805 21.065943793417148 56-57 21.353511501482284 28.8422871042649 28.81770500003678 20.986496394216033 58-59 21.267811297714417 28.7725254219417 28.853382767270613 21.106280513073266 60-61 21.388576248660552 28.77307713999171 28.95845440479431 20.87989220655343 62-63 21.243413099502963 28.847497774737196 28.933320582516174 20.97576854324366 64-65 21.278052650001868 28.620522870928845 28.765196047479773 21.33622843158951 66-67 21.18860910653553 28.86527535634856 28.80575110895328 21.14036442816263 68-69 21.23293045655282 28.545830605394084 29.08062930186824 21.140609636184855 70-71 21.289880019714722 28.430153720909136 28.9961551382115 21.28381112116464 72-73 21.08672517330077 28.656542027428973 29.00559564706719 21.25113715220307 74-75 21.40138836782184 28.548037477594118 28.575439474077836 21.47513468050621 76-77 21.126755382929108 28.415257333558923 29.082407060029375 21.37558022348259 78-79 21.31458472795396 28.42898898280356 28.690809848535004 21.565616440707476 80-81 21.26824041175331 28.64336209623434 28.593155753683636 21.495241738328712 82-83 21.3713503850992 28.743958687352418 28.6854765740516 21.19921435349679 84-85 21.62894141244725 28.5034709195546 28.587883781205786 21.279703886792362 86-87 20.944381916398775 28.633431171334202 29.03685966990096 21.385327242366063 88-89 21.03302461643335 28.65721634949009 29.00197882873936 21.3077802053372 90-91 21.15409607740727 28.733721252424495 28.686947822184948 21.425234847983287 92-93 21.214846364913676 28.670947998734725 28.674319609040328 21.43988602731127 94-95 21.275106236375628 28.448421718564944 28.716434086857586 21.560037958201843 96-97 21.096717400206465 28.6683120124958 28.948523479894167 21.286447107403568 98-99 21.117253572067867 28.491149216437766 28.67861074942928 21.712986462065093 100-101 21.17692999464831 28.55533234718417 28.765170012646657 21.502567645520855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 12.5 3 23.5 4 17.5 5 16.0 6 33.0 7 56.0 8 118.0 9 154.5 10 142.0 11 133.0 12 144.5 13 172.0 14 183.0 15 198.5 16 220.5 17 259.0 18 289.5 19 311.0 20 369.5 21 458.0 22 549.5 23 677.0 24 881.0 25 1103.0 26 1386.5 27 1842.5 28 2431.0 29 3193.5 30 4484.0 31 6797.0 32 10573.5 33 16557.0 34 24306.5 35 32875.0 36 43249.5 37 51530.5 38 55040.0 39 56570.5 40 53939.0 41 47647.0 42 41608.0 43 36795.0 44 33031.5 45 29619.0 46 26502.5 47 24257.5 48 22963.5 49 21714.5 50 20407.5 51 19036.0 52 17520.0 53 16351.0 54 15189.5 55 13669.0 56 11908.0 57 10148.0 58 8679.0 59 7245.5 60 5634.5 61 4222.5 62 3116.0 63 2213.0 64 1514.0 65 1011.5 66 703.0 67 471.5 68 322.5 69 243.5 70 177.0 71 121.0 72 86.0 73 68.0 74 45.5 75 29.0 76 19.0 77 14.5 78 12.0 79 6.5 80 3.5 81 1.0 82 1.5 83 2.5 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012996025177959723 2 0.10360038939033929 3 0.0 4 0.0 5 0.0 6 0.0035555163222719993 7 0.030405794755981236 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.001655154150023172 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 3.065100277820689E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.291140388948965E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 815634.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.184807202310914 #Duplication Level Percentage of deduplicated Percentage of total 1 75.27421855712929 41.539932383797954 2 13.57788663211021 14.985861120156734 3 4.30961965050012 7.134765885844191 4 2.0061929362239916 4.428454815846361 5 1.1572388786415355 3.1931002202425813 6 0.7415996711566304 2.4555020924445494 7 0.5178003214402768 2.0002297635583406 8 0.3673904103741327 1.6219495171579525 9 0.2934121702122801 1.4572704639578682 >10 1.6628597689532998 16.534159680373122 >50 0.07450242289427172 2.728493661108286 >100 0.016380991775598437 1.4892172595613524 >500 8.975885883926525E-4 0.4310631359507472 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 955 0.11708683061275031 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 915 0.11218267016823721 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 870 0.10666548966815999 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.130200555641379E-4 0.0 0.0 0.0 0.0 6 6.130200555641379E-4 0.0 0.0 0.0 0.0 7 6.130200555641379E-4 0.0 0.0 0.0 0.0 8 6.130200555641379E-4 0.0 0.0 0.0 0.0 9 6.130200555641379E-4 0.0 0.0 0.0 0.0 10-11 6.130200555641379E-4 0.0 0.0 1.2260401111282757E-4 0.0 12-13 6.130200555641379E-4 0.0 0.0 2.4520802222565514E-4 0.0 14-15 6.130200555641379E-4 0.0 0.0 2.4520802222565514E-4 0.0 16-17 6.130200555641379E-4 0.0 0.0 3.678120333384827E-4 0.0 18-19 6.130200555641379E-4 0.0 0.0 4.904160444513103E-4 0.0 20-21 6.130200555641379E-4 0.0 0.0 7.356240666769654E-4 0.0 22-23 6.130200555641379E-4 0.0 0.0 9.195300833462068E-4 0.0 24-25 6.130200555641379E-4 0.0 0.0 0.0011647381055718618 0.0 26-27 6.130200555641379E-4 0.0 0.0 0.001409946127797517 0.0 28-29 6.130200555641379E-4 0.0 0.0 0.0015938521444667584 0.0 30-31 6.130200555641379E-4 0.0 0.0 0.0021455701944744824 0.0 32-33 6.130200555641379E-4 0.0 0.0 0.00275859025003862 0.0 34-35 6.130200555641379E-4 0.0 0.0 0.003984630361166896 0.0 36-37 6.130200555641379E-4 0.0 0.0 0.006130200555641379 0.0 38-39 6.130200555641379E-4 0.0 0.0 0.010666548966815998 0.0 40-41 6.130200555641379E-4 0.0 0.0 0.015632011416885513 0.0 42-43 6.130200555641379E-4 0.0 0.0 0.02041356785028579 0.0 44-45 6.130200555641379E-4 0.0 0.0 0.026421164394814338 0.0 46-47 6.130200555641379E-4 0.0 0.0 0.03757812940608165 0.0 48-49 6.130200555641379E-4 0.0 0.0 0.04744775230066427 0.0 50-51 7.356240666769654E-4 0.0 0.0 0.059340341378608545 0.0 52-53 8.58228077789793E-4 0.0 0.0 0.0747271447732684 0.0 54-55 8.58228077789793E-4 0.0 0.0 0.0929951424290797 0.0 56-57 8.58228077789793E-4 0.0 0.0 0.11463475039049378 0.0 58-59 8.58228077789793E-4 0.0 0.0 0.13989117667973627 0.0 60-61 8.58228077789793E-4 0.0 0.0 0.16919353533570203 0.0 62-63 8.58228077789793E-4 0.0 0.0 0.20027365215280382 0.0 64-65 8.58228077789793E-4 0.0 0.0 0.2355836073532982 0.0 66-67 8.58228077789793E-4 0.0 0.0 0.27254871670381564 1.2260401111282757E-4 68-69 8.58228077789793E-4 0.0 0.0 0.3204255830433749 2.4520802222565514E-4 70-71 8.58228077789793E-4 0.0 0.0 0.379704622416427 2.4520802222565514E-4 72-73 8.58228077789793E-4 0.0 0.0 0.43732850763945597 2.4520802222565514E-4 74-75 8.58228077789793E-4 0.0 0.0 0.5034733716348264 2.4520802222565514E-4 76-77 8.58228077789793E-4 0.0 0.0 0.5860471731193158 2.4520802222565514E-4 78-79 8.58228077789793E-4 0.0 0.0 0.6813104897539828 2.4520802222565514E-4 80-81 8.58228077789793E-4 0.0 0.0 0.7842365570832015 2.4520802222565514E-4 82-83 8.58228077789793E-4 0.0 0.0 0.8953157911514233 2.4520802222565514E-4 84-85 8.58228077789793E-4 0.0 0.0 1.0234369827643282 2.4520802222565514E-4 86-87 8.58228077789793E-4 0.0 0.0 1.1577496769384306 2.4520802222565514E-4 88-89 8.58228077789793E-4 0.0 0.0 1.3148667171795192 2.4520802222565514E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 920 0.0 45.518826 1 CCGTTAT 85 3.6405072E-7 39.11553 9 TCAACGC 1610 0.0 34.527344 4 GTATCAA 1710 0.0 34.229866 1 ATCAACG 1625 0.0 34.208633 3 CAACGCA 1660 0.0 33.487366 5 ACCGTTA 100 1.2991295E-6 33.248196 8 CCGATCA 60 0.0039513335 31.66495 9 AACGCAG 1785 0.0 31.408487 6 CTTCGCG 40 4.4958864E-4 29.685888 22-23 TATCAAC 1970 0.0 28.257639 2 CGCAGAG 2015 0.0 27.107712 8 ACGCAGA 2075 0.0 27.018867 7 TACCGTT 125 7.372988E-6 26.606712 7 TAATACT 770 0.0 25.298592 4 GTACATG 2160 0.0 24.014244 1 ACATGGG 1975 0.0 23.816135 3 TCGTCCG 50 0.0016544639 23.748713 10-11 ATACCGT 165 2.423123E-6 23.036116 6 GTTACAC 395 0.0 22.853867 3 >>END_MODULE