FastQCFastQC Report
Fri 10 Feb 2017
SRR2568704_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568704_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665815
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC35350.034.8016934
GTATCAA38350.034.5557821
GGTATCA21500.034.243261
ATCAACG36300.033.8909033
CAACGCA37050.033.5894625
GTACATG85550.031.5917821
AACGCAG39750.031.5480926
TACATGG90500.029.181532
ACATGGG88550.028.7518793
TATCAAC44600.028.435332
ACGCAGA47400.025.95547
CGCAGAG48750.025.3340728
CATGGGG50700.023.3282094
TATAGTA11000.020.7267112
CATGGGA40800.020.7228664
GGACCGT3052.0918378E-1020.2464926
GAGTACA60850.019.9830251
ATGGGGG22850.019.7481945
TATACTG15200.019.374845
GCAGAGT62400.019.1071289
Produced by FastQC (version 0.11.5)