FastQCFastQC Report
Fri 10 Feb 2017
SRR2568704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665815
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.035.0878221
GTATCAA36400.031.4988041
TCAACGC33750.031.2549954
CAACGCA35150.030.1453135
ATCAACG35400.029.7981973
GTACATG81850.028.713541
AACGCAG37800.028.4090636
ACATGGG79950.027.1605133
TACATGG83150.027.0043492
TATCAAC44300.024.5915372
CATGGGG42100.023.4758594
ACGCAGA47050.022.821727
CGCAGAG47500.022.3987398
GTACGCT1353.7891095E-421.118243
GAGTACA56450.020.8166771
TACACCG3102.582965E-1019.9260835
GTATTAT13450.019.1007271
CATGGGA39600.018.5984524
GTATATA17200.018.5321061
AGTACAT58500.018.053072
Produced by FastQC (version 0.11.5)