FastQCFastQC Report
Fri 10 Feb 2017
SRR2568702_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568702_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369932
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG70700.030.6372622
GGTATCA17800.030.4227521
GTACATG75350.030.197151
TCAACGC25150.030.0293064
GTATCAA26850.029.7220441
CAACGCA25650.029.443945
ACATGGG71750.029.3270243
ATCAACG25900.028.9763373
AACGCAG27700.027.4363566
TATCAAC29750.025.5468582
CATGGGG43900.023.4790954
ACGCAGA35000.021.7139157
CGCAGAG36650.020.7363438
CATGGGA30750.020.0809564
TATAATA4750.020.0005022
GAGTACA52550.019.0731931
TATAGGA11250.019.0004772
GACCGCG2001.2858647E-518.9996747
TACACTG21850.018.912725
GTGTAGC15250.018.6893881
Produced by FastQC (version 0.11.5)