FastQCFastQC Report
Fri 10 Feb 2017
SRR2568702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369932
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT33220.1401727982068684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.029.966221
GTATCAA25900.028.6468181
TACATGG66800.027.6247062
TCAACGC25000.027.1779444
GTACATG72350.026.6894151
CAACGCA25350.026.6152745
ACATGGG68800.025.8978313
ATCAACG26250.025.8837553
AACGCAG27100.025.0719036
CATGGGG39200.023.1508964
TATCAAC30000.023.1458972
GAGTACA51800.020.1996781
ACGCAGA33250.020.1483217
TGGACCG4700.019.207745
AGTACAT51250.019.0240252
CGCAGAG35050.018.8365218
TACACTG25750.018.6365155
CATGGGA29150.018.2557644
TACCGTA3156.7921064E-918.1001477
GTACCGT4750.018.0052626
Produced by FastQC (version 0.11.5)