FastQCFastQC Report
Fri 10 Feb 2017
SRR2568701_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568701_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636655
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC30200.035.3873634
ATCAACG30150.035.1309743
CAACGCA31300.034.2954675
AACGCAG33400.032.566416
GTATCAA35050.032.1180731
GGTATCA21800.029.1969431
CGCAGAG36700.028.7321268
ACGCAGA37850.027.9846487
TATCAAC39350.027.521432
GTACATG60700.020.9717751
TATTACG1150.003725170920.6515672
GCAGAGT51650.019.9558539
ACATGGG58100.019.8656483
TATACGA2201.3467215E-619.430885
GTCGTAT1250.0060335318.9998041
TCTATAC15650.018.8169823
CTATACC13750.018.6536464
TACATGG64900.018.5164072
GTATATA24300.018.3742941
TACTATA13000.018.2686942
Produced by FastQC (version 0.11.5)