FastQCFastQC Report
Fri 10 Feb 2017
SRR2568701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2636655
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC28150.036.461884
ATCAACG28250.036.3328133
CAACGCA28700.035.9287075
AACGCAG30750.033.533466
GTATCAA32150.033.301761
GGTATCA22000.029.1995871
TATCAAC37250.028.0998672
CGCAGAG36600.027.7714638
ACGCAGA37050.027.7015827
TAGACCG1000.00165044423.7593044
GTATAGG15050.023.080861
GTACATG59200.022.1846731
ACATGGG56300.020.8474183
GCAGAGT51150.019.5930889
TACATGG63550.018.9414352
TAATACT25150.017.7604354
GACCCGT1350.0094069717.5914768
CTATACC13300.017.5068574
CGAATCG1102.9215149E-517.27195760-61
TCTATAC16950.017.1010933
Produced by FastQC (version 0.11.5)