##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568697_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 91705 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.440259527833813 33.0 31.0 34.0 30.0 34.0 2 31.44620249713756 33.0 31.0 34.0 30.0 34.0 3 31.442375006815332 34.0 31.0 34.0 28.0 34.0 4 34.81530996128892 37.0 35.0 37.0 32.0 37.0 5 34.92775748323428 37.0 35.0 37.0 32.0 37.0 6 35.19760100321684 37.0 35.0 37.0 33.0 37.0 7 35.19532195627283 37.0 35.0 37.0 33.0 37.0 8 35.20931246933101 37.0 35.0 37.0 33.0 37.0 9 36.9143667193719 39.0 38.0 39.0 33.0 39.0 10-11 36.90980317321847 39.0 37.5 39.0 33.5 39.0 12-13 36.88967886156698 39.0 38.0 39.0 33.0 39.0 14-15 38.016433127964675 40.0 38.0 41.0 33.0 41.0 16-17 38.02207077040511 40.0 38.0 41.0 33.0 41.0 18-19 38.193871653672105 41.0 38.5 41.0 33.5 41.0 20-21 38.25920615015539 41.0 39.0 41.0 34.0 41.0 22-23 38.08434109372444 40.5 38.5 41.0 34.0 41.0 24-25 37.955651273103975 40.5 38.0 41.0 34.0 41.0 26-27 37.6957472329753 40.0 37.0 41.0 33.0 41.0 28-29 37.450858731803066 40.0 36.0 41.0 33.0 41.0 30-31 36.989286298457 39.5 35.0 41.0 32.0 41.0 32-33 36.380584482852626 39.0 35.0 41.0 30.5 41.0 34-35 35.97889428057358 39.0 35.0 41.0 29.5 41.0 36-37 35.536911836868214 39.0 35.0 41.0 25.5 41.0 38-39 35.055351398506076 38.0 35.0 41.0 23.0 41.0 40-41 34.49425876451666 38.0 35.0 41.0 17.0 41.0 42-43 33.85198189847881 38.0 35.0 41.0 13.5 41.0 44-45 33.08775966414045 37.0 33.0 40.0 9.0 41.0 46-47 32.32775203096887 36.5 33.0 40.5 7.5 41.0 48-49 31.602835178016463 37.0 30.0 41.0 7.0 41.0 50-51 30.251736546535085 35.5 25.0 39.5 7.0 40.5 52-53 30.07178452647075 35.0 22.5 39.5 7.0 40.5 54-55 30.151049561092634 35.0 20.0 40.0 7.0 41.0 56-57 29.68367046507824 35.0 16.5 40.0 7.0 41.0 58-59 29.169238318521344 35.0 13.5 40.0 7.0 41.0 60-61 28.58261272558748 35.0 10.0 40.0 7.0 41.0 62-63 28.03973611035385 35.0 10.0 40.0 7.0 41.0 64-65 27.657150646093452 35.0 10.0 40.0 7.0 41.0 66-67 27.23618123330244 35.0 10.0 39.5 7.0 41.0 68-69 26.75761954091925 35.0 8.0 39.0 7.0 41.0 70-71 26.2484597350199 35.0 8.0 39.0 7.0 41.0 72-73 25.709519655416827 34.0 7.5 38.5 7.0 41.0 74-75 25.254991548988606 34.0 7.0 37.0 7.0 40.0 76-77 24.778021918106973 33.0 7.0 37.0 7.0 39.0 78-79 24.38970612289406 33.0 7.0 36.0 7.0 39.0 80-81 24.05283245188376 32.5 7.0 36.0 7.0 38.5 82-83 23.781178779782998 32.0 7.0 35.5 7.0 37.0 84-85 23.426263562510222 31.0 7.0 35.0 7.0 37.0 86-87 23.15508423750068 31.0 7.0 35.0 7.0 36.5 88-89 22.87777656616324 30.5 7.0 35.0 7.0 36.0 90-91 22.642505861185324 30.0 7.0 35.0 7.0 36.0 92-93 22.4322501499373 29.0 7.0 35.0 7.0 36.0 94-95 22.248950438907364 29.0 7.0 35.0 6.0 35.0 96-97 22.08028460825473 28.0 7.0 35.0 5.0 35.0 98-99 21.89115097322938 27.0 7.0 35.0 5.0 35.0 100-101 21.0348290714792 23.5 7.0 34.5 5.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11952990024022014 1101 2 0.14590329412062886 1101 3 -0.041715391271971214 1101 4 0.3628385823253879 1101 5 0.6381866931822913 1101 6 0.2826321897819071 1101 7 0.16006638520330085 1101 8 0.07727150656312887 1101 9 0.46293005608321636 1101 10-11 0.013087761985147495 1101 12-13 0.2160375090079043 1101 14-15 0.45201953515439897 1101 16-17 -0.385172384269282 1101 18-19 0.019612004571591513 1101 20-21 0.13572888912735692 1101 22-23 -0.043965923498319626 1101 24-25 0.047588551426841263 1101 26-27 0.18320129352982661 1101 28-29 -0.14673348522519092 1101 30-31 0.1358776178498644 1101 32-33 0.1977673019018127 1101 34-35 0.5502412867566733 1101 36-37 0.5784525631235127 1101 38-39 0.8231127975666368 1101 40-41 0.40412294819757477 1101 42-43 0.03775997183899449 1101 44-45 0.6545892792242043 1101 46-47 0.8153251429759649 1101 48-49 0.1127381656877624 1101 50-51 -0.5622341449091266 1101 52-53 -0.9786340002433249 1101 54-55 -0.7871515236717102 1101 56-57 -0.9168661920675767 1101 58-59 -1.7538081773717629 1101 60-61 -1.0274352611964659 1101 62-63 -1.2099242898144134 1101 64-65 -1.9266476728913382 1101 66-67 -2.2568247679233586 1101 68-69 -2.2285588173437105 1101 70-71 -2.0822317189374715 1101 72-73 -2.108395007707543 1101 74-75 -2.093089260001772 1101 76-77 -2.2630813247344364 1101 78-79 -2.122015509734304 1101 80-81 -0.6078008509855621 1101 82-83 -1.2738738028834469 1101 84-85 -1.6124739226154752 1101 86-87 -0.411603125019127 1101 88-89 -0.4125827102718276 1101 90-91 -1.0352079488847714 1101 92-93 -1.2578395234240531 1101 94-95 -1.262284538994404 1101 96-97 -1.2254238784530607 1101 98-99 -1.119314129474656 1101 100-101 -0.45823662315134683 1102 1 -0.7184400245718088 1102 2 -0.5770290367064383 1102 3 -0.368407120595279 1102 4 -0.40746216955431436 1102 5 -0.25467044967485464 1102 6 -0.03541292300005239 1102 7 -0.3606103065260271 1102 8 0.13667000280373287 1102 9 0.08135110871479867 1102 10-11 -0.22657389215019208 1102 12-13 -0.6764436939996159 1102 14-15 -0.47091279567267463 1102 16-17 -0.7581047150963514 1102 18-19 -0.2065158149772799 1102 20-21 -0.8056244943312834 1102 22-23 -0.34039449492689755 1102 24-25 -1.0234640801521024 1102 26-27 -0.7921746463198005 1102 28-29 -0.5424101769545118 1102 30-31 -0.5351750137290807 1102 32-33 -0.6770447281733709 1102 34-35 -2.0157361568523555 1102 36-37 -1.148427136124603 1102 38-39 -0.6866616385235886 1102 40-41 -0.5095988563137084 1102 42-43 0.14584267860590927 1102 44-45 -0.5922152320539951 1102 46-47 -1.0091109472495994 1102 48-49 -0.3944046914550938 1102 50-51 0.0041192385495172346 1102 52-53 -0.251942270920015 1102 54-55 -0.31835453119050783 1102 56-57 -0.09374589131569877 1102 58-59 0.053146709846281226 1102 60-61 -0.1193525544295504 1102 62-63 -0.6967663950775709 1102 64-65 -1.4516476728913368 1102 66-67 -1.3273134897278709 1102 68-69 -0.8928445316294216 1102 70-71 -1.4123069069073964 1102 72-73 -0.7661017746248397 1102 74-75 -1.7229764780468848 1102 76-77 -1.9211640315013518 1102 78-79 -1.65848167514784 1102 80-81 -2.675845963767518 1102 82-83 -2.6836482389736744 1102 84-85 -2.4275115166004397 1102 86-87 -2.1469414708837924 1102 88-89 -2.112206770422201 1102 90-91 -2.119606445125374 1102 92-93 -1.6839673429729274 1102 94-95 -1.0974349149342544 1102 96-97 -2.0263637280771185 1102 98-99 -1.712359242256614 1102 100-101 -1.359928352474654 1103 1 0.5427441859545077 1103 2 0.5262604369777719 1103 3 0.6368560372994558 1103 4 0.5806957251825295 1103 5 0.5953295503251468 1103 6 0.3665607612104722 1103 7 0.3939949566318717 1103 8 0.41298579227741783 1103 9 0.27543005608321636 1103 10-11 0.33362347627086564 1103 12-13 0.5946089375793306 1103 14-15 0.9198766780115406 1103 16-17 0.6701847585878582 1103 18-19 0.9553262902858748 1103 20-21 0.5473360319845071 1103 22-23 0.44085550507310245 1103 24-25 0.45383855142684126 1103 26-27 0.12427272210126006 1103 28-29 0.9121950862033756 1103 30-31 0.7608776178498644 1103 32-33 0.7397315876160988 1103 34-35 0.6770270010423829 1103 36-37 0.8775597059806586 1103 38-39 1.0097199404237784 1103 40-41 0.8157300910547178 1103 42-43 0.17972425755328203 1103 44-45 0.2304821363670655 1103 46-47 0.6849680001188219 1103 48-49 1.0368453085449048 1103 50-51 0.9386587122337282 1103 52-53 1.2865445711852495 1103 54-55 1.0655270477568628 1103 56-57 1.5634909507895678 1103 58-59 1.1988703940568115 1103 60-61 1.3395290245178195 1103 62-63 0.9838257101855881 1103 64-65 0.6796023271086646 1103 66-67 1.4565680892195019 1103 68-69 1.7696554683705799 1103 70-71 1.5847325667768146 1103 72-73 1.5335692780067411 1103 74-75 0.8533393114268009 1103 76-77 0.8012043895512768 1103 78-79 0.04941306169426696 1103 80-81 0.5011277204430087 1103 82-83 0.48773333997369406 1103 84-85 -0.025866779758334957 1103 86-87 0.1553611606951577 1103 88-89 -0.4536541388432589 1103 90-91 0.2415777654009439 1103 92-93 0.18412476229023156 1103 94-95 0.28414403243416686 1103 96-97 0.7835046929755123 1103 98-99 0.6208644419539127 1103 100-101 -0.22341519457991765 1104 1 0.15524418595450484 1104 2 0.4012604369777719 1104 3 0.6493560372994587 1104 4 0.08069572518252954 1104 5 0.49532955032514536 1104 6 0.2665607612104779 1104 7 0.7939949566318703 1104 8 0.212985792277415 1104 9 0.36293005608321494 1104 10-11 0.03362347627086848 1104 12-13 0.3008589375793349 1104 14-15 0.12612667801153776 1104 16-17 0.09518475858786246 1104 18-19 0.2053262902858748 1104 20-21 0.6223360319845028 1104 22-23 0.8346055050730996 1104 24-25 0.4100885514268455 1104 26-27 0.0742727221012558 1104 28-29 0.6746950862033785 1104 30-31 0.1983776178498644 1104 32-33 0.47723158761610307 1104 34-35 0.9707770010423857 1104 36-37 1.1150597059806628 1104 38-39 0.9284699404237813 1104 40-41 0.7469800910547164 1104 42-43 -0.16402574244671797 1104 44-45 -0.15076786363294303 1104 46-47 -0.7837819998811781 1104 48-49 -0.7194046914550931 1104 50-51 -0.8050912877662704 1104 52-53 -0.3384554288147541 1104 54-55 -0.2969729522431379 1104 56-57 -0.07400904921043505 1104 58-59 0.11137039405680937 1104 60-61 -0.7917209754821819 1104 62-63 -0.8286742898144119 1104 64-65 -1.314147672891334 1104 66-67 -1.4871819107804995 1104 68-69 -1.211594531629423 1104 70-71 -1.5465174332231832 1104 72-73 -1.8414307219932589 1104 74-75 -1.8216606885731998 1104 76-77 -1.4362956104487203 1104 78-79 -1.519336938305731 1104 80-81 -1.7488722795569913 1104 82-83 -1.9622666600263052 1104 84-85 -2.044616779758332 1104 86-87 -1.3633888393048466 1104 88-89 -1.4474041388432575 1104 90-91 -1.7959222345990575 1104 92-93 -1.6783752377097727 1104 94-95 -1.4846059675658339 1104 96-97 -1.728995307024487 1104 98-99 -1.8853855580460852 1104 100-101 -1.0296651945799162 1105 1 0.3688805495908696 1105 2 0.5948210430383796 1105 3 1.0929166433600663 1105 4 0.31781693730373917 1105 5 0.4801780351736298 1105 6 0.541939549089264 1105 7 0.6708888960258079 1105 8 0.9205615498531756 1105 9 0.5992936924468495 1105 10-11 0.469797718695105 1105 12-13 1.1309725739429695 1105 14-15 1.3903312234660845 1105 16-17 1.0349574858605877 1105 18-19 1.2689626539222374 1105 20-21 1.3028284562269263 1105 22-23 0.7467267171943135 1105 24-25 0.8369824908207804 1105 26-27 1.0860151463436765 1105 28-29 1.2125738740821603 1105 30-31 1.1661806481528956 1105 32-33 1.7077240118585237 1105 34-35 1.2751330616484466 1105 36-37 1.3713097059806572 1105 38-39 1.1538487283025631 1105 40-41 1.4591013031759275 1105 42-43 1.3765045605835766 1105 44-45 1.2253685000034196 1105 46-47 2.0997407273915485 1105 48-49 2.8783225812721795 1105 50-51 2.899454166779183 1105 52-53 2.529158207548889 1105 54-55 2.74185280533262 1105 56-57 3.062354587153205 1105 58-59 2.202468878905293 1105 60-61 2.0204002366390306 1105 62-63 2.7521969223067977 1105 64-65 2.7642614180177567 1105 66-67 2.2594089983104055 1105 68-69 2.160185771400883 1105 70-71 2.6080280213222693 1105 72-73 2.681864732552196 1105 74-75 3.390460523548011 1105 76-77 3.234537722884614 1105 78-79 3.264564576845782 1105 80-81 3.2272640840793727 1105 82-83 3.51292273391309 1105 84-85 3.215231705090151 1105 86-87 2.7210808576648517 1105 88-89 3.2406640429749274 1105 90-91 3.2215020078251833 1105 92-93 2.8293899138053824 1105 94-95 3.439447062737198 1105 96-97 2.5838834808543005 1105 98-99 2.92938716922664 1105 100-101 2.8324560175412934 1106 1 0.09783677854709794 1106 2 -0.25198030376297 1106 3 -0.3526501355400491 1106 4 -0.6844277316075988 1106 5 -0.1528185978229999 1106 6 -0.7891491153327337 1106 7 -0.898134672997756 1106 8 -0.6950388990806076 1106 9 -0.6049711784846821 1106 10-11 -0.6892931903958086 1106 12-13 -0.3695885932848668 1106 14-15 -0.7785801121119178 1106 16-17 -0.402191784622012 1106 18-19 -0.6101058084795525 1106 20-21 -0.5998090297438878 1106 22-23 -0.2709500504824547 1106 24-25 -0.1641398436348851 1106 26-27 -0.887609993948125 1106 28-29 -0.5999962718213112 1106 30-31 0.12661835859060488 1106 32-33 -0.1517035975690888 1106 34-35 0.3472430504250994 1106 36-37 0.5750134096843595 1106 38-39 0.9462168540040281 1106 40-41 1.4599430540176783 1106 42-43 1.4932273439730324 1106 44-45 1.164201272169528 1106 46-47 2.443455654439809 1106 48-49 2.491397777680703 1106 50-51 1.3183655023571887 1106 52-53 1.6520538304445083 1106 54-55 2.197240010719824 1106 56-57 2.959941568073514 1106 58-59 2.8410771841802642 1106 60-61 3.394698777604237 1106 62-63 2.728347315123859 1106 64-65 2.7659449197012584 1106 66-67 2.550935373170116 1106 68-69 3.323204851086629 1106 70-71 2.9991615791224966 1106 72-73 3.3816402656610656 1106 74-75 3.6721664719206295 1106 76-77 3.225278463625351 1106 78-79 2.8821291110769813 1106 80-81 2.943874634023256 1106 82-83 2.7856500066403633 1106 84-85 3.4001363066614196 1106 86-87 2.7555926421766372 1106 88-89 2.5526730216505733 1106 90-91 2.6364851728083494 1106 92-93 2.0328130338951667 1106 94-95 1.7085267484835498 1106 96-97 2.252794816432303 1106 98-99 2.5014971580032963 1106 100-101 2.080211348629959 1107 1 0.5393350950454128 1107 2 0.40921498243231724 1107 3 0.8277651282085507 1107 4 0.014786634273434629 1107 5 0.6316931866887785 1107 6 0.6176971248468419 1107 7 0.6974040475409637 1107 8 0.7198039740955977 1107 9 1.0197482379013962 1107 10-11 0.36373711263450303 1107 12-13 0.6821089375793292 1107 14-15 0.37896758710244427 1107 16-17 0.5690483949515013 1107 18-19 0.35418992664951077 1107 20-21 0.38426785016631726 1107 22-23 0.2618782323458291 1107 24-25 0.1873612786995693 1107 26-27 0.08222726755580112 1107 28-29 0.703104177112472 1107 30-31 0.5563321633044112 1107 32-33 0.6300724967070082 1107 34-35 0.40770881922420443 1107 36-37 -0.35028120311024935 1107 38-39 -1.0715300595762187 1107 40-41 -0.36097445439983034 1107 42-43 -0.4549348333558143 1107 44-45 -0.8257678636329402 1107 46-47 -1.3491229089720882 1107 48-49 -0.9512228732732737 1107 50-51 -1.0266821968571769 1107 52-53 -0.9253872469965714 1107 54-55 -0.9759502249704113 1107 56-57 -1.261509049210435 1107 58-59 -1.390334151397738 1107 60-61 -0.9871755209367272 1107 62-63 -0.8974242898144134 1107 64-65 -0.28687494561861016 1107 66-67 -0.6553637289623175 1107 68-69 -0.8473899861748748 1107 70-71 -1.0567447059504573 1107 72-73 -0.24995344926598406 1107 74-75 -0.17393341584592648 1107 76-77 -0.08743197408508507 1107 78-79 -0.1710414837602805 1107 80-81 -0.3238722795569906 1107 82-83 -0.023062114571757775 1107 84-85 -0.3207531433947004 1107 86-87 -0.5497524756684804 1107 88-89 -0.7422905024796194 1107 90-91 -0.9243313255081524 1107 92-93 -0.9641706922552231 1107 94-95 -1.5340377857476533 1107 96-97 -0.8233134888426683 1107 98-99 -0.8944764671369931 1107 100-101 -0.49898337639809753 1108 1 0.21952990024022156 1108 2 0.6206285688459019 1108 3 0.3879549383983587 1108 4 0.4023990218858273 1108 5 0.39093394592953956 1108 6 0.30351131066102255 1108 7 0.6435828687197827 1108 8 0.13551326480489223 1108 9 0.49040258355574196 1108 10-11 0.1971536960510818 1108 12-13 0.3407627837331759 1108 14-15 0.46575579889066177 1108 16-17 0.08241003331313124 1108 18-19 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66-67 -1.4583026004356725 2316 68-69 -1.8790514281811461 2316 70-71 -1.1741036401197356 2316 72-73 -1.1347496875104994 2316 74-75 -0.4664882747800938 2316 76-77 -1.3074163001038919 2316 78-79 -0.7902421107195288 2316 80-81 -1.14326883128113 2316 82-83 -0.6292925220952696 2316 84-85 -0.9851340211376431 2316 86-87 -0.9638198737876031 2316 88-89 -0.42736103539497705 2316 90-91 -0.4157498208059529 2316 92-93 -0.8928148928821855 2316 94-95 -1.0787870020485926 2316 96-97 -1.286753927714141 2316 98-99 -1.1375407304598788 2316 100-101 -0.7641479532006095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 142.0 8 408.0 9 352.0 10 341.0 11 420.0 12 415.0 13 402.0 14 341.0 15 466.0 16 1047.0 17 2184.0 18 2190.0 19 2735.0 20 3603.0 21 4486.0 22 3565.0 23 3152.0 24 3505.0 25 2675.0 26 2276.0 27 1973.0 28 1821.0 29 1441.0 30 1312.0 31 1345.0 32 1442.0 33 1769.0 34 2412.0 35 3824.0 36 5063.0 37 9901.0 38 18749.0 39 5935.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.65606290282125 18.71598852743274 12.59692247292714 15.03102609681887 2 13.869743500261734 27.92815389984296 40.18823067527482 18.013871924620485 3 22.36349955835687 37.542937526580374 27.33825501891977 12.755307896142984 4 10.461058646485355 19.03993369828357 38.998058930012434 31.50094872521864 5 8.651130328575011 46.81403286840642 30.78223317593431 13.752603627084264 6 20.81070470457371 39.95506990337848 20.74963467033087 18.48459072171694 7 22.347514095335725 39.42986139132142 23.888458728202664 14.33416578514019 8 28.558497644390158 30.36010294887454 23.727316349677192 17.354083057058105 9 33.05705810504275 14.372491711743152 21.761036468330133 30.809413714883966 10-11 21.958733655408576 31.812382084473867 26.482872940227054 19.746011319890506 12-13 27.384107043543693 30.47022387978321 27.052049595969507 15.093619480703591 14-15 25.318684913581592 28.454827981026114 30.26770623193937 15.958780873452918 16-17 17.322937680606294 32.79210511967723 32.08331061556077 17.801646584155716 18-19 16.67584469682513 31.023559367315674 37.962150167112846 14.338445768746356 20-21 17.406357341475385 30.63682460062156 38.042091489013686 13.914726568889375 22-23 16.643585409737746 31.19404612616542 36.22594187885066 15.936426585246169 24-25 15.444676477484009 32.15977928387213 35.5245006897379 16.871043548905963 26-27 16.00680490956777 31.680452788213547 36.11782090219579 16.194921400022903 28-29 16.158845788939903 31.58104232325325 37.09037175977928 15.169740128027568 30-31 15.635752886067806 36.1073393644925 35.58547504921448 12.671432700225212 32-33 13.339585298431375 36.51892764259115 36.679225119814184 13.462261939163293 34-35 12.535303418570415 36.18614034131182 36.96581429583992 14.312741944277848 36-37 13.84875415735238 36.1490649364811 35.40701161332534 14.595169292841176 38-39 14.289297202987841 35.41464478490813 35.850280791668936 14.44577722043509 40-41 13.413118150591572 35.507333296984896 36.40423095796304 14.675317594460498 42-43 14.107268454655989 36.518382413076786 33.553969543479326 15.8203795887879 44-45 13.728312668346037 37.39708400126498 34.10213629077109 14.77246703961789 46-47 12.74911260025845 36.057600558339374 35.22936079955944 15.963926041842738 48-49 13.204687099570878 36.51968134702312 33.42802772128224 16.847603832123752 50-51 13.36226873940141 35.91661804718725 32.34676685005752 18.374346363353816 52-53 12.996019846246115 34.94084292023336 32.76102720680443 19.3021100267161 54-55 13.38803772967668 35.11258928084619 32.05986587427076 19.439507115206368 56-57 13.326427130472712 33.475273976337164 31.31236028569871 21.88593860749141 58-59 13.427839267215527 31.84450139032768 32.49114006869854 22.236519273758244 60-61 14.6889482580012 32.20871272013522 31.301455754866147 21.800883266997438 62-63 14.085298030577304 30.479706004231094 33.9670890493119 21.4679069158797 64-65 13.946640930430373 28.916188201680487 33.95056679698363 23.18660407090551 66-67 14.696452524018799 28.516668665961458 34.52327724402665 22.263601565993085 68-69 14.025770075957924 28.547747708448068 35.067696888069754 22.358785327524252 70-71 14.95207354175981 26.692147476091293 36.41702016291725 21.938758819231648 72-73 14.515566217763482 27.52194536830053 34.02377187721498 23.938716536721007 74-75 14.458862657434162 26.671937189902405 34.1562619268306 24.712938225832833 76-77 14.686767351834687 25.089144539556184 36.26029115097323 23.963796957635896 78-79 13.943078349054034 25.878087345291966 36.38787416171419 23.790960143939806 80-81 14.381533528555304 26.56382067935064 35.05052322759718 24.004122564496868 82-83 14.30682487403756 25.876284162540625 35.13588675376797 24.68100420965385 84-85 14.086442507988789 26.806338681004675 32.90671930723844 26.200499503768093 86-87 14.545593351583033 26.82106204534796 32.20108843834183 26.432256164727182 88-89 14.74572204469359 25.608838381084293 32.132924714530326 27.512514859691787 90-91 15.53897831871919 23.876128778955636 32.38341403830214 28.201478864023034 92-93 16.264347446767903 25.65119005425448 30.25164262929742 27.832819869680197 94-95 16.68693721946191 23.658189394228618 30.393285083963722 29.261588302345746 96-97 17.110249573320683 23.52652500368062 29.240483551716807 30.122741871281892 98-99 17.307493060970756 23.32277255797975 28.17000485323067 31.199729527818825 100-101 17.362118419474104 22.24809414228223 28.55078470078852 31.839002737455147 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 78.0 1 59.0 2 134.5 3 265.5 4 198.5 5 111.5 6 181.0 7 313.0 8 410.5 9 379.5 10 288.0 11 247.5 12 253.0 13 259.5 14 279.5 15 290.0 16 300.5 17 301.0 18 334.5 19 383.0 20 399.5 21 437.5 22 507.5 23 621.0 24 757.0 25 913.0 26 1148.5 27 1442.5 28 1697.5 29 1976.0 30 2600.0 31 3272.5 32 3997.5 33 4793.0 34 5266.0 35 5712.5 36 5972.5 37 5370.5 38 4383.5 39 3782.0 40 3281.0 41 3100.0 42 2577.0 43 2032.0 44 1962.0 45 1692.5 46 1317.0 47 1039.5 48 1214.5 49 1608.0 50 2185.5 51 2034.0 52 1385.0 53 1168.5 54 920.0 55 704.0 56 552.0 57 414.5 58 337.5 59 279.0 60 256.5 61 304.5 62 289.0 63 173.5 64 96.0 65 90.0 66 97.0 67 82.0 68 63.0 69 54.0 70 42.0 71 51.5 72 57.0 73 39.0 74 25.5 75 16.5 76 14.0 77 10.0 78 8.0 79 10.5 80 9.5 81 7.5 82 5.0 83 4.0 84 2.5 85 1.0 86 1.0 87 1.0 88 3.0 89 2.5 90 1.0 91 1.0 92 1.0 93 1.5 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008723624666048743 2 0.009814077749304836 3 0.002180906166512186 4 0.003271359249768279 5 0.006542718499536558 6 0.00763317158279265 7 0.008723624666048743 8 0.009814077749304836 9 0.009814077749304836 10-11 0.008723624666048743 12-13 0.004361812333024372 14-15 0.0 16-17 0.0 18-19 5.452265416280465E-4 20-21 0.0 22-23 0.0 24-25 0.003816585791396325 26-27 0.007087945041164603 28-29 0.006542718499536558 30-31 0.015811569707213348 32-33 5.452265416280465E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 5.452265416280465E-4 44-45 0.004361812333024372 46-47 0.004907038874652418 48-49 0.007087945041164603 50-51 0.007087945041164603 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.003271359249768279 64-65 0.005997491957908511 66-67 0.006542718499536558 68-69 0.010359304290932882 70-71 0.002180906166512186 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.015811569707213348 82-83 0.011994983915817022 84-85 0.0152663431655853 86-87 0.0152663431655853 88-89 0.0152663431655853 90-91 0.014175890082329206 92-93 0.008178398124420696 94-95 0.031077912872798645 96-97 0.00926885120767679 98-99 0.014721116623957255 100-101 0.0152663431655853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 91705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.223870017992475 #Duplication Level Percentage of deduplicated Percentage of total 1 80.05097976740481 27.39654326372608 2 7.780787000159312 5.3257728586227575 3 3.04604110243747 3.1274194427784745 4 1.7078222080611758 2.337931410501063 5 1.2075832404014657 2.066408592770296 6 0.8316074557909829 1.7076495283790416 7 0.6627369762625458 1.5876996892208715 8 0.47156284849450375 1.291096450575214 9 0.3887207264616855 1.19731748541519 >10 3.2021666401147044 22.73158497355651 >50 0.4078381392384897 9.577449430238264 >100 0.2230364823960491 14.407066135979498 >500 0.006372470925601402 1.372880431819421 >1k 0.012744941851202804 5.873180306417317 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 2.113298075350308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1308 1.4263126328989695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1079 1.1765988768333242 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1061 1.1569707213347147 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.8265634371081184 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 501 0.5463169947113026 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 497 0.5419551823782781 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 494 0.5386838231285099 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 489 0.5332315577122294 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 487 0.5310506515457172 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 359 0.39147265688893734 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 330 0.35984951747451066 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 322 0.35112589280846196 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 322 0.35112589280846196 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 315 0.34349272122566926 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 309 0.3369500027261327 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 286 0.3118695818112426 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 276 0.30096505097868165 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 270 0.29442233247914507 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 258 0.28133689548007196 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 246 0.26825145848099885 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 242 0.2638896461479745 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 234 0.25516602148192574 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 230 0.2508042091489014 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 223 0.2431710375661087 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 218 0.23771877214982823 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 203 0.22136197590098683 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 182 0.19846246115260893 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 181 0.19737200806935282 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 179 0.1951911019028406 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 177 0.19301019573632844 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 174 0.18973883648656015 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 171 0.18646747723679188 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 168 0.18319611798702362 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 167 0.1821056649037675 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 160 0.17447249332097486 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 157 0.17120113407120657 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 155 0.1690202279046944 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 154 0.1679297748214383 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 154 0.1679297748214383 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 153 0.16683932173818222 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.16683932173818222 No Hit GTACATGGGAGGATTAAACTCAGATGCAGATAAAATTCCATTTCACCCCT 152 0.1657488686549261 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 152 0.1657488686549261 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 141 0.15375388473910911 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 140 0.152663431655853 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 139 0.1515729785725969 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 137 0.14939207240608474 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 135 0.14721116623957253 No Hit GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA 132 0.14393980698980427 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 132 0.14393980698980427 No Hit ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.13739708849026772 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 125 0.1363066354070116 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 122 0.13303527615724334 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 120 0.13085436999073113 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 120 0.13085436999073113 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 120 0.13085436999073113 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 120 0.13085436999073113 No Hit GTACATGAGCTCATCATAGCCTTATAGAAGGTAAACGAAACCACATAAAT 119 0.12976391690747505 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 119 0.12976391690747505 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 118 0.12867346382421896 No Hit GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA 117 0.12758301074096287 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 116 0.12649255765770678 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 114 0.12431165149119458 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 114 0.12431165149119458 No Hit ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 113 0.1232211984079385 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 113 0.1232211984079385 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 112 0.1221307453246824 No Hit CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.12104029224142632 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 110 0.11994983915817022 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 110 0.11994983915817022 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 108 0.11776893299165804 No Hit ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG 103 0.11231666757537757 No Hit GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT 103 0.11231666757537757 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 102 0.11122621449212147 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 102 0.11122621449212147 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 100 0.1090453083256093 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.1090453083256093 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 100 0.1090453083256093 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 99 0.10795485524235318 No Hit TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA 99 0.10795485524235318 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 98 0.10686440215909711 No Hit TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT 97 0.105773949075841 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 97 0.105773949075841 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.105773949075841 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 95 0.10359304290932883 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 95 0.10359304290932883 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.001090453083256093 0.0 8 0.0 0.0 0.0 0.001090453083256093 0.0 9 0.0 0.0 0.0 0.001090453083256093 0.0 10-11 0.0 0.0 0.0 0.001090453083256093 0.0 12-13 0.0 0.0 0.0 0.0027261327081402323 0.0 14-15 0.0 0.0 0.0 0.004907038874652418 0.0 16-17 0.0 0.0 0.0 0.014721116623957255 0.0 18-19 0.0 0.0 0.0 0.025625647456518186 0.0 20-21 0.0 0.0 0.0 0.051796521454664415 0.0 22-23 0.0 0.0 0.0 0.1090453083256093 0.0 24-25 0.0 0.0 0.0 0.18701270377841994 0.0 26-27 0.0 0.0 0.0 0.24262581102448066 0.0 28-29 0.0 0.0 0.0 0.3205932064772913 0.0 30-31 0.0 0.0 0.0 0.4394525925522055 0.0 32-33 0.0 0.0 0.0 0.6111989531650401 0.0 34-35 0.0 0.001090453083256093 0.0 0.8407393271904476 0.0 36-37 0.0 0.001090453083256093 0.0 1.117169183795867 0.0 38-39 0.0 0.001090453083256093 0.0 1.4775639278120059 0.0 40-41 0.0 0.001090453083256093 0.0 2.006978899732839 0.0 42-43 0.0 0.001090453083256093 0.0 2.3057630445450084 0.0 44-45 0.0 0.001090453083256093 0.0 2.5113134507387818 0.0 46-47 0.0 0.001090453083256093 0.0 2.7583010740962868 0.0 48-49 0.0 0.001090453083256093 0.0 3.002562564745652 0.0 50-51 0.0 0.001090453083256093 0.0 3.266997437435254 0.0 52-53 0.0 0.001090453083256093 0.0 3.5630554495392834 0.0 54-55 0.0 0.001090453083256093 0.0 3.790414917398179 0.0 56-57 0.0 0.001090453083256093 0.0 3.953982879886593 0.0 58-59 0.0 0.001090453083256093 0.0 4.155171473747342 0.0 60-61 0.0 0.001090453083256093 0.0 4.350362575650182 0.0 62-63 0.0 0.001090453083256093 0.0 4.547189357177908 0.0 64-65 0.0 0.001090453083256093 0.0 4.763644294204242 0.0 66-67 0.0 0.001090453083256093 0.0 4.958290169565455 0.0 68-69 0.0 0.001090453083256093 0.0 5.18837577013249 0.0 70-71 0.0 0.001090453083256093 0.0 5.492066953819312 0.0 72-73 0.0 0.001090453083256093 0.0 5.765770677716591 0.0 74-75 0.0 0.001090453083256093 0.0 6.019301019573632 0.0 76-77 0.0 0.001090453083256093 0.0 6.28537157188812 0.0 78-79 0.0 0.001090453083256093 0.0 6.573251185867728 0.0 80-81 0.0 0.001090453083256093 0.0 6.878578049179434 0.0 82-83 0.0 0.001090453083256093 0.0 7.202442614906493 0.0 84-85 0.0 0.001090453083256093 0.0 7.51594787634262 0.0 86-87 0.0 0.001090453083256093 0.0 7.801101357614089 0.0 88-89 0.0 0.001090453083256093 0.0 8.12442069679952 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 30 1.2201781E-8 94.99019 2 ATAAACG 20 1.736137E-5 94.99019 3 ACCTGGG 30 1.3581375E-6 79.158485 3 TAAACGC 25 5.2521696E-5 75.99215 4 CATGAGC 35 3.3940887E-6 67.850136 4 TACATGA 35 3.3940887E-6 67.850136 2 TGGGATG 80 0.0 65.305756 6 GGGATGC 70 1.8189894E-11 61.06512 7 ATATAGG 25 0.00500864 56.99411 2 TATAGGC 25 0.00500864 56.99411 3 ATGGGAT 120 0.0 55.410942 5 GGGTGCG 35 2.775818E-4 54.280106 7 TATATGG 35 2.775818E-4 54.280106 2 GGATGCC 80 6.91216E-11 53.43198 8 GAGATGG 45 1.505876E-5 52.772327 5 TGGGTGC 40 5.364855E-4 47.495094 6 AAACGCA 40 5.364855E-4 47.495094 5 GGTGCGT 40 5.364855E-4 47.495094 8 GTATAAA 40 5.364855E-4 47.495094 1 GTGCGTT 40 5.364855E-4 47.495094 9 >>END_MODULE