FastQCFastQC Report
Fri 10 Feb 2017
SRR2568697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91705
Sequences flagged as poor quality0
Sequence length101
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20662.252876070007088No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT17181.8733983970339676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16031.747996292459517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12151.3249004961561528No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA9481.0337495229267761No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9271.0108500081783982No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.7022517856169239No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5350.5833923995420097No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT4920.5365029169619977No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4690.5114224960471075No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT4540.4950656997982662TruSeq Adapter, Index 3 (95% over 21bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA4190.45689984188430294No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3490.3805681260563764No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3470.37838721988986423No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.37620631372335206No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3260.3554877051414863No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG3070.33476909655962056TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT3030.33040728422659615RNA PCR Primer, Index 3 (95% over 23bp)
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2980.3249550188103157No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2930.3195027533940352No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT2780.3031459571451938No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2770.30205550406193776No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA2690.293331879395889No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2670.29115097322937683No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC2590.2824273485633281No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA2570.2802464423968159No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.2475328498991331No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA2170.23662831906657217No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT2140.23335695981680388No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2050.22354288206749903No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT1810.19737200806935282No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT1790.1951911019028406No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1770.19301019573632844No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA1760.19191974265307235No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC1760.19191974265307235No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA1710.18646747723679188No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.18428657107027968No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC1670.1821056649037675No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC1630.17774385257074315No Hit
CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC1620.17665339948748704No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1620.17665339948748704No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.17556294640423098No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA1560.1701106809879505No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT1550.1690202279046944No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC1520.1657488686549261No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA1510.16465841557167005No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1500.16356796248841393No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG1480.16138705632190176No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC1470.16029660323864564No Hit
GTACATGGGAGGATTAAACTCAGATGCAGATAAAATTCCATTTCACCCCT1450.15811569707213347No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.15702524398887738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1430.1559347909056213No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC1410.15375388473910911No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG1340.14612071315631645No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG1330.14503026007306036No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT1310.14284935390654818No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA1310.14284935390654818No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA1300.14175890082329207No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1300.14175890082329207No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT1270.1384875415735238No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT1180.12867346382421896No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.12867346382421896No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA1150.1254021045744507No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG1130.1232211984079385No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA1130.1232211984079385No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.1221307453246824No Hit
GTACATGAGCTCATCATAGCCTTATAGAAGGTAAACGAAACCACATAAAT1110.12104029224142632No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA1080.11776893299165804No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1080.11776893299165804No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1080.11776893299165804No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1080.11776893299165804No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC1070.11667847990840194No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT1040.11340712065863365TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA1040.11340712065863365RNA PCR Primer, Index 3 (96% over 28bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1030.11231666757537757No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG1030.11231666757537757No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG1030.11231666757537757No Hit
TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA1020.11122621449212147No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA1020.11122621449212147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1010.11013576140886538No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA990.10795485524235318No Hit
GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT990.10795485524235318No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC980.10686440215909711No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG960.10468349599258493No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA960.10468349599258493No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT960.10468349599258493No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA950.10359304290932883No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT940.10250258982607273No Hit
GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA940.10250258982607273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG930.10141213674281664No Hit
GATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCT930.10141213674281664No Hit
GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA920.10032168365956054No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC920.10032168365956054No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGT156.571124E-495.09881
GTACAGG156.571124E-495.09881
ACTGAGT156.5854046E-495.046916
CTTGGGC156.599708E-494.9950948
TACCTGG506.366463E-1176.079042
ACCTGGG506.366463E-1176.037543
TATTGTC200.002059460971.3241042
GTCTTGG200.00206392771.285196
TCTTGGG200.00206392771.285197
GGGAGTC200.002068400471.246329
GGGTGGC200.002068400471.246328
AGGCTTA659.094947E-1265.801716
TATAGGC659.094947E-1265.801713
ATATAGG701.8189894E-1161.134942
GATATAG801.8189894E-1259.4367521
TACAGGG250.00498601957.059282
CTGAGTG250.004996808757.028157
GCGTGCA250.005007616256.9970559
CTTACTA851.2551027E-1050.291529
AAGTACT405.334684E-447.54942
Produced by FastQC (version 0.11.5)