##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568697_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 91705 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.412725587481596 33.0 31.0 34.0 31.0 34.0 2 32.39248677825636 33.0 31.0 34.0 31.0 34.0 3 32.430172836813696 34.0 31.0 34.0 30.0 34.0 4 35.935783217927046 37.0 35.0 37.0 35.0 37.0 5 36.091096450575215 37.0 35.0 37.0 35.0 37.0 6 36.21203860203915 37.0 37.0 37.0 35.0 37.0 7 36.124180797121205 37.0 36.0 37.0 35.0 37.0 8 36.1618886647402 37.0 37.0 37.0 35.0 37.0 9 37.91364702033695 39.0 38.0 39.0 35.0 39.0 10-11 37.83842756665395 39.0 38.0 39.0 35.0 39.0 12-13 37.82274685131672 39.0 38.0 39.0 35.0 39.0 14-15 38.978240008723624 40.5 38.0 41.0 35.5 41.0 16-17 38.959478763426205 40.0 38.5 41.0 35.5 41.0 18-19 39.235407011613326 41.0 39.0 41.0 36.0 41.0 20-21 39.24830161932283 41.0 39.0 41.0 36.0 41.0 22-23 39.07589553459462 41.0 38.5 41.0 35.0 41.0 24-25 38.969734474674226 41.0 38.0 41.0 35.0 41.0 26-27 38.66169238318521 40.0 38.0 41.0 35.0 41.0 28-29 38.43551060465624 40.0 37.0 41.0 35.0 41.0 30-31 37.93990513058176 40.0 35.5 41.0 34.5 41.0 32-33 37.256420042527665 39.0 35.0 41.0 33.0 41.0 34-35 36.89901859222507 39.0 35.0 41.0 33.0 41.0 36-37 36.4354669865329 39.0 35.0 41.0 32.0 41.0 38-39 35.815222725042254 39.0 35.0 41.0 30.0 41.0 40-41 35.330936153971976 38.0 35.0 41.0 25.5 41.0 42-43 34.747325663813314 38.0 35.0 41.0 20.0 41.0 44-45 34.04738018646748 38.0 34.5 41.0 13.5 41.0 46-47 33.10055067880704 38.0 33.0 41.0 9.0 41.0 48-49 32.222943132871706 37.5 32.5 41.0 7.0 41.0 50-51 31.475192192355923 37.0 30.0 40.0 7.0 41.0 52-53 31.221051196772258 37.0 27.5 41.0 7.0 41.0 54-55 30.821100267161007 37.0 23.5 41.0 7.0 41.0 56-57 30.292034240226815 36.0 21.0 40.5 7.0 41.0 58-59 29.7802082765389 35.5 17.5 40.0 7.0 41.0 60-61 29.238122239790634 35.0 13.5 40.0 7.0 41.0 62-63 28.68607491412682 35.0 10.0 40.0 7.0 41.0 64-65 28.21492285044436 35.0 10.0 40.0 7.0 41.0 66-67 27.62349926394417 35.0 10.0 39.5 7.0 41.0 68-69 26.98708358322883 35.0 10.0 39.0 7.0 41.0 70-71 26.406221034839977 35.0 9.0 39.0 7.0 41.0 72-73 25.8630281882122 34.5 8.0 37.5 7.0 40.5 74-75 25.425587481598605 34.0 8.0 37.0 7.0 40.0 76-77 23.993102884248408 31.0 7.5 35.5 7.0 39.0 78-79 24.578725260345674 33.0 7.0 36.0 7.0 39.0 80-81 24.46801155880268 33.0 7.0 36.0 7.0 39.0 82-83 24.296041655307782 33.0 7.0 35.5 7.0 37.0 84-85 23.992792105119676 33.0 7.0 35.0 7.0 37.0 86-87 23.80288970067063 33.0 7.0 35.0 7.0 36.5 88-89 23.603364047761843 33.0 7.0 35.0 7.0 36.0 90-91 23.42905512240336 32.0 7.0 35.0 7.0 36.0 92-93 23.318668556785347 32.0 7.0 35.0 7.0 36.0 94-95 23.20947058502808 32.0 7.0 35.0 7.0 35.5 96-97 23.092824818712174 31.0 7.0 35.0 7.0 35.0 98-99 23.038149501117715 31.0 7.0 35.0 5.5 35.0 100-101 22.369254675317592 28.5 7.0 34.5 5.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8044060525602603 1101 2 0.5685417425524903 1101 3 0.34956254823843835 1101 4 0.5974596497361517 1101 5 0.5651467174311264 1101 6 0.441518729475213 1101 7 0.08458845489676037 1101 8 0.030101961584009018 1101 9 0.5680762815908977 1101 10-11 0.6048632255167661 1101 12-13 0.5692491198882195 1101 14-15 0.8127448371344244 1101 16-17 0.7350071359844037 1101 18-19 0.4981893345979955 1101 20-21 0.5110949326745668 1101 22-23 0.5657267576656935 1101 24-25 0.7579683852496615 1101 26-27 0.7573889578085087 1101 28-29 1.0745330341595434 1101 30-31 0.681119641423706 1101 32-33 0.32641279973895365 1101 34-35 0.13046184492319668 1101 36-37 0.25008868869869616 1101 38-39 0.39788858749695066 1101 40-41 0.5336200816832886 1101 42-43 1.4362475819606217 1101 44-45 1.4127972741878594 1101 46-47 1.3552793226262594 1101 48-49 1.1774364085654412 1101 50-51 1.5931979959865608 1101 52-53 1.3737319783200377 1101 54-55 1.304740778063561 1101 56-57 0.7207215568169261 1101 58-59 0.960003805016072 1101 60-61 0.11449721275202407 1101 62-63 -0.3614340151744173 1101 64-65 -0.4197598064296777 1101 66-67 -0.3289998698995724 1101 68-69 -0.7865865879673954 1101 70-71 -0.6375472437858996 1101 72-73 -0.27078867811781393 1101 74-75 -0.43622555865362855 1101 76-77 -0.4126242841314749 1101 78-79 0.14633124373991535 1101 80-81 0.37094114086261953 1101 82-83 0.1955632018435054 1101 84-85 -0.4050416701586492 1101 86-87 0.18607493275471398 1101 88-89 -0.13451091847555574 1101 90-91 0.19608538200731118 1101 92-93 0.25239801659131444 1101 94-95 -0.2690059757063814 1101 96-97 -0.12273894491104187 1101 98-99 -0.7379194298764453 1101 100-101 -0.4335615506512269 1102 1 0.21831582699634566 1102 2 0.28884249443218835 1102 3 0.15257006703542686 1102 4 -0.0792320795871575 1102 5 -0.13372546301999932 1102 6 0.20317286481355978 1102 7 0.3691749210621751 1102 8 0.36732000669679366 1102 9 0.36619658234278063 1102 10-11 0.12629179694533832 1102 12-13 0.25364761612882347 1102 14-15 0.2215794236005948 1102 16-17 0.5836913465107187 1102 18-19 0.3643547481318308 1102 20-21 0.3289520755317099 1102 22-23 0.4818921711995259 1102 24-25 0.3564646258511601 1102 26-27 0.30663707810926155 1102 28-29 0.46457062814451433 1102 30-31 0.6322474609725788 1102 32-33 0.888818814776549 1102 34-35 0.44888289755477473 1102 36-37 1.0873067338114808 1102 38-39 1.057287083737549 1102 40-41 1.0682065478487033 1102 42-43 0.9065483338403197 1102 44-45 0.7032108080224475 1102 46-47 0.6951289466864097 1102 48-49 0.2234890401443863 1102 50-51 0.8777844621519684 1102 52-53 -0.09506501416116464 1102 54-55 -0.7567253873499702 1102 56-57 -0.5059701725063803 1102 58-59 -0.4086052175403161 1102 60-61 -0.8464050428870742 1102 62-63 -0.8830505565278024 1102 64-65 -1.168819956805617 1102 66-67 -1.4096389676439323 1102 68-69 -1.8644061368395803 1102 70-71 -0.9409307024325138 1102 72-73 -0.7777435653358573 1102 74-75 -1.2503233030145289 1102 76-77 -0.9566092465374894 1102 78-79 -0.8382552224254951 1102 80-81 -2.170412242596022 1102 82-83 -2.107632286878303 1102 84-85 -1.481357459632335 1102 86-87 -1.4936243153655866 1102 88-89 -0.9518041515582638 1102 90-91 -1.8195161217520877 1102 92-93 -1.927489201453799 1102 94-95 -1.8389307877364551 1102 96-97 -1.7787539825050267 1102 98-99 -1.8886713095756917 1102 100-101 -1.822095385237695 1103 1 0.39726319541740196 1103 2 0.450684599695343 1103 3 0.345991119667012 1103 4 0.2635310783075795 1103 5 0.329432431716846 1103 6 0.24330444376092686 1103 7 -0.031482973674670234 1103 8 -0.3734694669874159 1103 9 -0.08906657555196063 1103 10-11 -0.01745820305465884 1103 12-13 0.22371340560250985 1103 14-15 0.5600662657058564 1103 16-17 0.3037571359844051 1103 18-19 0.14461790602656777 1103 20-21 0.18520207553171275 1103 22-23 0.18715532909426003 1103 24-25 0.18475409953537536 1103 26-27 0.20828181495136278 1103 28-29 0.5754258913024017 1103 30-31 0.4704053557094241 1103 32-33 0.5835556568818063 1103 34-35 0.7679618449231995 1103 36-37 0.16526726012726556 1103 38-39 -0.2663971267887604 1103 40-41 -0.1949513468881392 1103 42-43 -0.24410956089652558 1103 44-45 -0.10863129724071285 1103 46-47 -0.12329210594516837 1103 48-49 0.314936408565444 1103 50-51 0.5708765674151302 1103 52-53 0.7398034068914683 1103 54-55 0.28241934949213743 1103 56-57 0.5492929853883588 1103 58-59 0.568039519301788 1103 60-61 0.34931864132345325 1103 62-63 0.25017312768272504 1103 64-65 0.4525616221417543 1103 66-67 0.8058215586718553 1103 68-69 1.0901991263183177 1103 70-71 0.7320956133569574 1103 72-73 0.7515327504536167 1103 74-75 1.0352030127749465 1103 76-77 1.0614828587256682 1103 78-79 0.10615267231134595 1103 80-81 0.476298283719764 1103 82-83 0.25717034470064704 1103 84-85 1.1574583298413472 1103 86-87 1.211967789897571 1103 88-89 1.7922747958101581 1103 90-91 0.9255496677215973 1103 92-93 0.6988265880198874 1103 94-95 0.9890297385793296 1103 96-97 0.4031539122318186 1103 98-99 0.4254734272664109 1103 100-101 1.4637598779202037 1104 1 -0.002736804582596619 1104 2 0.15068459969534587 1104 3 0.020991119667009173 1104 4 0.12603107830758375 1104 5 -0.08306756828315542 1104 6 -0.01919555623906888 1104 7 0.006017026325331187 1104 8 0.17653053301258126 1104 9 0.3484334244480394 1104 10-11 0.11379179694533548 1104 12-13 0.3549634056025113 1104 14-15 0.36006626570586064 1104 16-17 0.0475071359844037 1104 18-19 -0.2553820939734308 1104 20-21 0.18520207553171275 1104 22-23 0.005905329094268552 1104 24-25 -0.027745900464630324 1104 26-27 0.0582818149513642 1104 28-29 -0.218324108697594 1104 30-31 -0.04834464429057306 1104 32-33 -0.34769434311818515 1104 34-35 0.01796184492319952 1104 36-37 -0.05973273987272876 1104 38-39 -0.13514712678876606 1104 40-41 -0.11995134688813636 1104 42-43 0.2496404391034801 1104 44-45 0.5101187027592928 1104 46-47 0.851707894054833 1104 48-49 0.7899364085654383 1104 50-51 0.9958765674151344 1104 52-53 0.7710534068914683 1104 54-55 0.38241934949213885 1104 56-57 0.4617929853883531 1104 58-59 1.130539519301788 1104 60-61 0.34306864132345183 1104 62-63 -0.18107687231727354 1104 64-65 0.008811622141752906 1104 66-67 -0.20667844132814395 1104 68-69 -0.6410508736816816 1104 70-71 -0.3616543866430426 1104 72-73 -0.9297172495463819 1104 74-75 -1.4147969872250563 1104 76-77 -0.1822671412743304 1104 78-79 -0.23759732768865405 1104 80-81 -0.27995171628023385 1104 82-83 -0.586579655299353 1104 84-85 -0.8050416701586514 1104 86-87 0.1119677898975695 1104 88-89 -0.8389752041898433 1104 90-91 -1.586950332278402 1104 92-93 -1.401173411980114 1104 94-95 -0.7984702614206682 1104 96-97 -1.090596087768187 1104 98-99 -1.0182765727335905 1104 100-101 -1.0674901220797963 1105 1 0.44120258935679146 1105 2 0.14689672090747052 1105 3 0.6270517257276182 1105 4 0.2987583510348557 1105 5 0.2733718256562341 1105 6 0.31641050436699203 1105 7 0.40147157177987935 1105 8 0.21062144210348777 1105 9 0.38972130323591614 1105 10-11 0.1490190696726117 1105 12-13 -0.01927901863991366 1105 14-15 0.32464959903918356 1105 16-17 0.5077344087116771 1105 18-19 0.704466390875055 1105 20-21 0.19637631795595212 1105 22-23 0.12503411697305467 1105 24-25 0.14195106923234135 1105 26-27 0.2902893907089421 1105 28-29 0.4316758913024046 1105 30-31 0.24389020419426544 1105 32-33 0.753252626578778 1105 34-35 1.4365224509838086 1105 36-37 1.5290930177030262 1105 38-39 1.5305725701809294 1105 40-41 2.123230471293674 1105 42-43 2.0524813481943838 1105 44-45 2.265611127001712 1105 46-47 2.6448897122366475 1105 48-49 2.821375802504832 1105 50-51 2.8115962643848285 1105 52-53 3.0265458311338875 1105 54-55 3.238290561613347 1105 56-57 3.7659596520550203 1105 58-59 3.5485319435442086 1105 60-61 4.087008035262844 1105 62-63 4.031423127682725 1105 64-65 3.175478288808417 1105 66-67 3.45260186170216 1105 68-69 3.508002156621348 1105 70-71 3.148383492144834 1105 72-73 3.5199039625748263 1105 74-75 3.706415133987065 1105 76-77 3.492354070846879 1105 78-79 3.190054187462863 1105 80-81 3.6956164655379453 1105 82-83 3.961147617427919 1105 84-85 3.340412875295897 1105 86-87 2.568975365655149 1105 88-89 2.088865704901064 1105 90-91 2.5817996677215973 1105 92-93 2.754129618322917 1105 94-95 2.640734284033879 1105 96-97 2.484403912231816 1105 98-99 2.8677082757512586 1105 100-101 2.3950098779202023 1106 1 0.5102261583803624 1106 2 0.31580805648547283 1106 3 0.4222256875682433 1106 4 0.4334384857149871 1106 5 0.16338304900079237 1106 6 0.3977797524029043 1106 7 0.41157258188088974 1106 8 0.48054287869159396 1106 9 0.5171062639542114 1106 10-11 0.5460448833650915 1106 12-13 0.4203955043679386 1106 14-15 0.5715631792861018 1106 16-17 0.4479700989473656 1106 18-19 0.5282598813352166 1106 20-21 0.7348162730625702 1106 22-23 0.639624464896734 1106 24-25 0.02101953163413839 1106 26-27 0.39971700013655465 1106 28-29 0.7958734221666006 1106 30-31 0.3892325162032435 1106 32-33 0.3854075087336568 1106 34-35 0.9909556720836932 1106 36-37 0.5554678774112176 1106 38-39 0.160763367038399 1106 40-41 0.41615976422297507 1106 42-43 0.5291929082392741 1106 44-45 0.8753193200432392 1106 46-47 1.3112757952894043 1106 48-49 1.5570660381950745 1106 50-51 1.552898172353398 1106 52-53 2.0992169871383766 1106 54-55 2.560119966776085 1106 56-57 2.6641078002031726 1106 58-59 2.689104334116603 1106 60-61 3.066525431446909 1106 62-63 2.0252502881765544 1106 64-65 2.6075770542405188 1106 66-67 2.4396950154619788 1106 68-69 2.863501595454121 1106 70-71 3.0041635145915286 1106 72-73 3.122597565268432 1106 74-75 4.446314123886058 1106 76-77 3.3172698957627063 1106 78-79 3.5452730426817176 1106 80-81 3.387255073843221 1106 82-83 3.160642566922867 1106 84-85 3.2517484532981413 1106 86-87 3.147538777551894 1106 88-89 3.4822439316126257 1106 90-91 3.3379725072277715 1106 92-93 3.1960488102421074 1106 94-95 3.292810602776864 1106 96-97 3.141811319639224 1106 98-99 2.6272481186244363 1106 100-101 2.512293828537487 1107 1 -0.1800095318553261 1107 2 -0.47431540030465413 1107 3 0.06644566512155592 1107 4 0.04875835103485571 1107 5 0.12943243171684315 1107 6 -0.10783191987543717 1107 7 -0.1439829736746674 1107 8 -0.23256037607832525 1107 9 0.21547887899349405 1107 10-11 -0.09529911214556819 1107 12-13 -0.009809321670225302 1107 14-15 -0.22459282520323853 1107 16-17 -0.014992864015596297 1107 18-19 -0.0644730030643359 1107 20-21 0.056224802804436536 1107 22-23 0.5019280563669923 1107 24-25 0.11543591771718553 1107 26-27 0.28839545131499733 1107 28-29 0.32372134584785783 1107 30-31 -0.22391282610876573 1107 32-33 -0.10848979766364408 1107 34-35 -0.5672654278040739 1107 36-37 -0.5693918307818251 1107 38-39 -0.2459425813342193 1107 40-41 0.06641228947550104 1107 42-43 0.012708620921657143 1107 44-45 0.7106868845774699 1107 46-47 1.048298803145741 1107 48-49 0.9785727722018009 1107 50-51 -0.33991888713032026 1107 52-53 -1.3749693203812612 1107 54-55 -1.8829215595987705 1107 56-57 -1.020025196429824 1107 58-59 -0.5859377534254868 1107 60-61 -0.4148859041310935 1107 62-63 0.20187767313727178 1107 64-65 0.24744798577811622 1107 66-67 -0.015201168600871284 1107 68-69 -0.2779826918635031 1107 70-71 0.36618652244786887 1107 72-73 0.23960093227179868 1107 74-75 0.3692939218658502 1107 76-77 0.1836419496347581 1107 78-79 0.24308449049316394 1107 80-81 -0.20779262537114107 1107 82-83 -0.7774887462084479 1107 84-85 -0.5004962156131967 1107 86-87 -0.45564584646606576 1107 88-89 -0.32022520418984257 1107 90-91 -0.36706396864203583 1107 92-93 -0.4864006847073874 1107 94-95 -0.24562935232976102 1107 96-97 -0.17468699685909073 1107 98-99 0.2635416090845908 1107 100-101 0.7643280597383821 1108 1 0.33847198662618894 1108 2 0.16304723705798096 1108 3 -0.21527261659672803 1108 4 -0.09484804257154167 1108 5 -0.04364449136007664 1108 6 0.1184418063982946 1108 7 0.10876427907258801 1108 8 -0.02264529116324354 1108 9 0.0219224354370553 1108 10-11 -0.011070840417303884 1108 12-13 0.00661175725085883 1108 14-15 0.38142615581574546 1108 16-17 0.1937983447756153 1108 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-1.1892665274896892 2316 64-65 -1.5015332054444563 2316 66-67 -1.957324993052282 2316 68-69 -1.9240250116127164 2316 70-71 -2.102818179746489 2316 72-73 -1.8818724219601783 2316 74-75 -1.3096245734319503 2316 76-77 -1.2887326585157126 2316 78-79 -2.0802697414817573 2316 80-81 -1.7982706817974758 2316 82-83 -1.5857175863338355 2316 84-85 -2.0602140839517524 2316 86-87 -2.1102304859645002 2316 88-89 -1.7512596869484618 2316 90-91 -1.5371658495197806 2316 92-93 -1.2537596188766642 2316 94-95 -1.3392030200413565 2316 96-97 -1.8173202256992198 2316 98-99 -2.1066386416991065 2316 100-101 -0.9394728807004888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 4.0 14 52.0 15 173.0 16 673.0 17 1679.0 18 2328.0 19 2636.0 20 3511.0 21 4579.0 22 4303.0 23 3241.0 24 3331.0 25 2942.0 26 2646.0 27 2509.0 28 2462.0 29 2004.0 30 1431.0 31 1319.0 32 1351.0 33 1567.0 34 1993.0 35 3382.0 36 4518.0 37 10250.0 38 20167.0 39 6644.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.43802689409224 18.355817783254995 12.80359460373202 16.402560718920746 2 13.07727580409197 27.532371116011966 41.86518767604865 17.525165403847414 3 21.436126710648274 37.205168747614636 29.331007033422384 12.027697508314706 4 9.459680497246605 20.450357123384766 38.17785289787906 31.91210948148956 5 7.843629027861077 46.04874325282155 32.45297421078458 13.654653508532796 6 19.93609457131017 40.6900913869441 20.40393465506336 18.96987938668237 7 20.65704656261248 39.33553657711899 23.81357503572091 16.193841824547626 8 27.612452974210782 31.064827435799575 24.272395180197375 17.05032440979227 9 31.42903876560711 13.38858295621831 21.894117005615836 33.28826127255875 10-11 20.891445395561856 31.179325009541465 27.868164222234338 20.06106537266234 12-13 26.56343710811842 29.3468186031296 28.178398124420696 15.911346164331281 14-15 24.85905893898915 27.065590752957853 31.100267161005394 16.9750831470476 16-17 17.218799411155334 32.30576304454501 32.61054468131508 17.86489286298457 18-19 16.741180960689167 28.902458971702742 39.060574668774876 15.295785398833214 20-21 16.657216073278448 28.470094324191702 39.7840902895153 15.088599313014559 22-23 15.937517038329426 30.29769369172891 37.83708630936154 15.92770296058012 24-25 15.967504498118966 30.989586173054906 37.42162368464097 15.621285644185159 26-27 16.31372335205278 30.58938989149992 38.33487814186795 14.762008614579358 28-29 16.42739917233257 29.03815012513154 39.32619801861434 15.208252683921552 30-31 15.127855624011776 34.29856605419552 38.67564473038548 11.897933591407229 32-33 13.774058121149338 34.877051414862876 38.839212692873886 12.509677771113898 34-35 13.690093233738617 33.12360285698708 39.57526852407175 13.611035385202552 36-37 13.362957308761791 34.96319720844011 37.395998037184455 14.277847445613654 38-39 14.066844774003599 32.37827817458154 38.58404667139197 14.970830380022901 40-41 12.684150264434873 32.45788124965923 39.63088163131781 15.227086854588082 42-43 14.66877487596096 33.24355269614525 36.713374407066134 15.374298020827654 44-45 13.119241044654053 35.092961125347585 37.18063355324137 14.607164276756993 46-47 13.178125511149883 32.94149719208331 36.74608799956382 17.134289297202987 48-49 13.688457554113734 33.29643967068317 35.19546371517365 17.819639060029445 50-51 13.130145575486615 33.245188375770134 34.43378223651927 19.19088381222398 52-53 12.593097431982988 32.74139905130582 35.71997164821983 18.945531868491358 54-55 12.915326318085166 33.15958780873453 33.510168475001365 20.414917398178943 56-57 13.752249059484214 32.19181069734475 31.27583010740963 22.780110135761408 58-59 12.559838612943677 31.37724224415245 33.89073660105774 22.172182541846137 60-61 15.01499372989477 33.19448230739873 30.721334714573906 21.0691892481326 62-63 13.155225996401503 32.86898206204678 33.22556022027152 20.75023172128019 64-65 12.664591159648655 30.908515939784852 33.240462572719984 23.18643032784651 66-67 13.537975028624395 30.36257565018265 33.71462842811188 22.384820893081077 68-69 13.330243716264109 29.907311487923234 34.24022681424132 22.522217981571345 70-71 14.492666703015104 27.840902895152936 35.51278556240118 22.153644839430783 72-73 14.35417916144158 28.497355651273104 33.161768714901044 23.986696472384274 74-75 14.603892917507224 27.549206695381933 32.98129872962216 24.865601657488686 76-77 14.613706995256528 26.14415789760646 35.67471784526471 23.567417261872308 78-79 13.97143012921869 27.038874652418084 35.54877051414863 23.440924704214602 80-81 14.552641622594187 28.003380404558094 34.00905076059102 23.434927212256692 82-83 14.78872471511913 27.298947712774655 34.20478708903549 23.707540483070716 84-85 15.103865656180144 27.893789869690856 31.792704868873017 25.209639605255983 86-87 15.906984351998254 28.084073932719043 30.67553568507715 25.333406030205552 88-89 15.66817512676517 27.487596096177963 30.278065536230304 26.56616324082656 90-91 16.4249495665449 26.10653726623412 30.250258982607274 27.218254184613706 92-93 16.48928629845701 27.8790687530669 28.306526361703288 27.325118586772806 94-95 16.66866583065264 26.16215037348018 28.627119568180582 28.542064227686602 96-97 17.592279592170545 26.163240826563438 27.90905621285644 28.33542336840957 98-99 18.1435036257565 26.294095196554167 26.978899732838997 28.583501444850334 100-101 19.02009748863178 25.444096703488434 27.229207332366446 28.30659847551334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 67.0 1 56.0 2 174.5 3 321.5 4 215.5 5 102.5 6 161.5 7 310.5 8 403.5 9 372.0 10 298.0 11 243.5 12 248.5 13 253.0 14 282.0 15 319.0 16 332.0 17 351.0 18 374.0 19 386.0 20 399.0 21 427.0 22 456.5 23 562.0 24 690.0 25 798.5 26 973.0 27 1190.5 28 1447.0 29 1769.5 30 2371.0 31 3069.0 32 3922.0 33 4788.0 34 5390.5 35 5983.5 36 6230.0 37 5660.0 38 4676.0 39 3997.5 40 3553.0 41 3405.0 42 2861.0 43 2276.5 44 2197.5 45 1924.5 46 1473.5 47 1164.0 48 1346.0 49 1707.5 50 2435.0 51 2310.5 52 1224.5 53 759.0 54 597.0 55 458.5 56 361.0 57 302.5 58 240.5 59 156.5 60 157.5 61 211.0 62 163.5 63 76.0 64 40.0 65 43.0 66 49.0 67 35.0 68 20.0 69 16.5 70 16.0 71 23.0 72 28.0 73 17.5 74 8.0 75 3.0 76 1.5 77 1.0 78 0.5 79 0.0 80 1.0 81 1.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013085436999073117 2 0.12104029224142632 3 0.0 4 0.0 5 0.0 6 0.00763317158279265 7 0.023989967831634044 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0016356796248841396 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.452265416280465E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.002180906166512186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 91705.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.34621885393381 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80732329584247 23.61158061174418 2 8.6169104171907 5.229812987296222 3 3.3993316324697256 3.094705850280792 4 1.8002802831578568 2.1852679788452103 5 1.2253404721693197 1.8592225069516384 6 0.9486506881310863 1.727277683877651 7 0.74382838046642 1.5800665176380786 8 0.6324337920873908 1.5353579412245788 9 0.48510546552157824 1.3249004961561528 >10 3.5790003234036436 22.48623302982389 >50 0.47432534406554316 9.978736164876507 >100 0.25872291494484173 14.857423259364266 >500 0.014373495274713429 3.330243716264108 >1k 0.014373495274713429 7.199171255656725 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2066 2.252876070007088 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 1718 1.8733983970339676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1603 1.747996292459517 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 1.3249004961561528 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 948 1.0337495229267761 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 927 1.0108500081783982 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 644 0.7022517856169239 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 535 0.5833923995420097 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 492 0.5365029169619977 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 469 0.5114224960471075 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 454 0.4950656997982662 TruSeq Adapter, Index 3 (95% over 21bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 419 0.45689984188430294 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 349 0.3805681260563764 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 347 0.37838721988986423 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.37620631372335206 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 326 0.3554877051414863 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 307 0.33476909655962056 TruSeq Adapter, Index 3 (96% over 28bp) ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 303 0.33040728422659615 RNA PCR Primer, Index 3 (95% over 23bp) CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 298 0.3249550188103157 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 293 0.3195027533940352 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 278 0.3031459571451938 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 277 0.30205550406193776 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 269 0.293331879395889 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 267 0.29115097322937683 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 259 0.2824273485633281 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 257 0.2802464423968159 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 227 0.2475328498991331 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 217 0.23662831906657217 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 214 0.23335695981680388 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 205 0.22354288206749903 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 181 0.19737200806935282 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 179 0.1951911019028406 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 177 0.19301019573632844 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 176 0.19191974265307235 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 176 0.19191974265307235 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 171 0.18646747723679188 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 169 0.18428657107027968 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 167 0.1821056649037675 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 163 0.17774385257074315 No Hit CTACTATACAATCAAAGATATCCTAGGTATCCTAATCATATTCTTAATTC 162 0.17665339948748704 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 162 0.17665339948748704 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.17556294640423098 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 156 0.1701106809879505 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 155 0.1690202279046944 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 152 0.1657488686549261 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 151 0.16465841557167005 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 150 0.16356796248841393 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 148 0.16138705632190176 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 147 0.16029660323864564 No Hit GTACATGGGAGGATTAAACTCAGATGCAGATAAAATTCCATTTCACCCCT 145 0.15811569707213347 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 144 0.15702524398887738 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 143 0.1559347909056213 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 141 0.15375388473910911 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 134 0.14612071315631645 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 133 0.14503026007306036 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT 131 0.14284935390654818 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 131 0.14284935390654818 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 130 0.14175890082329207 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 130 0.14175890082329207 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 127 0.1384875415735238 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 118 0.12867346382421896 No Hit GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 118 0.12867346382421896 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 115 0.1254021045744507 No Hit ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG 113 0.1232211984079385 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 113 0.1232211984079385 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.1221307453246824 No Hit GTACATGAGCTCATCATAGCCTTATAGAAGGTAAACGAAACCACATAAAT 111 0.12104029224142632 No Hit CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.11776893299165804 No Hit ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.11776893299165804 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 108 0.11776893299165804 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 108 0.11776893299165804 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 107 0.11667847990840194 No Hit CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT 104 0.11340712065863365 TruSeq Adapter, Index 3 (96% over 26bp) CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA 104 0.11340712065863365 RNA PCR Primer, Index 3 (96% over 28bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 103 0.11231666757537757 No Hit GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG 103 0.11231666757537757 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 103 0.11231666757537757 No Hit TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA 102 0.11122621449212147 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 102 0.11122621449212147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 101 0.11013576140886538 No Hit ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 99 0.10795485524235318 No Hit GTATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAAT 99 0.10795485524235318 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 98 0.10686440215909711 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 96 0.10468349599258493 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 96 0.10468349599258493 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 96 0.10468349599258493 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 95 0.10359304290932883 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 94 0.10250258982607273 No Hit GCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGCCATTGGTTGAATAAA 94 0.10250258982607273 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 93 0.10141213674281664 No Hit GATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCT 93 0.10141213674281664 No Hit GGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGA 92 0.10032168365956054 No Hit GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC 92 0.10032168365956054 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.001090453083256093 0.0 8 0.0 0.0 0.0 0.001090453083256093 0.0 9 0.0 0.0 0.0 0.001090453083256093 0.0 10-11 0.0 0.0 0.0 0.001090453083256093 0.0 12-13 0.0 0.0 0.0 0.0027261327081402323 0.0 14-15 0.0 0.0 0.0 0.0054522654162804645 0.0 16-17 0.0 0.0 0.0 0.014175890082329208 0.0 18-19 0.0 0.0 0.0 0.025625647456518186 0.0 20-21 0.0 0.0 0.0 0.053432201079548554 0.0 22-23 0.0 0.0 0.0 0.11013576140886538 0.0 24-25 0.0 0.0 0.0 0.1886483834033041 0.0 26-27 0.0 0.0 0.0 0.24698762335750507 0.0 28-29 0.0 0.0 0.0 0.3216836595605474 0.0 30-31 0.0 0.0 0.0 0.4383621394689493 0.0 32-33 0.0 0.0 0.0 0.6090180469985279 0.0 34-35 0.0 0.0 0.0 0.8412845537320757 0.0 36-37 0.0 0.0 0.0 1.117169183795867 0.0 38-39 0.0 0.0 0.0 1.4759282481871216 0.0 40-41 0.0 0.0 0.0 2.0020718608581864 0.0 42-43 0.0 0.0 0.0 2.3035821383784962 0.0 44-45 0.0 0.0 0.0 2.51185867728041 0.0 46-47 0.0 0.0 0.0 2.761572433346055 0.0 48-49 0.0 0.0 0.0 3.005288697453792 0.0 50-51 0.0 0.0 0.0 3.2724497028515347 0.0 52-53 0.0 0.0 0.0 3.5668720353306798 0.0 54-55 0.0 0.0 0.0 3.7942315031895752 0.0 56-57 0.0 0.0 0.0 3.9605255983861296 0.0 58-59 0.0 0.0 0.0 4.160623739163622 0.0 60-61 0.0 0.0 0.0 4.361812333024371 0.0 62-63 0.0 0.0 0.0 4.560274794176981 0.0 64-65 0.0 0.0 0.0 4.775094051578431 0.0 66-67 0.0 0.0 0.0 4.975192192355924 0.0 68-69 0.0 0.0 0.0 5.207458699089472 0.0 70-71 0.0 0.0 0.0 5.515511695109318 0.0 72-73 0.0 0.0 0.0 5.793577231339622 0.0 74-75 0.0 0.0 0.0 6.054740744779457 0.0 76-77 0.0 0.0 0.0 6.328444468676736 0.0 78-79 0.0 0.0 0.0 6.626138160405649 0.0 80-81 0.0 0.0 0.0 6.933100703342239 0.0 82-83 0.0 0.0 0.0 7.259691401777438 0.0 84-85 0.0 0.0 0.0 7.572651436671936 0.0 86-87 0.0 0.0 0.0 7.856169238318522 0.0 88-89 0.0 0.0 0.0 8.183850389836977 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTGT 15 6.571124E-4 95.0988 1 GTACAGG 15 6.571124E-4 95.0988 1 ACTGAGT 15 6.5854046E-4 95.04691 6 CTTGGGC 15 6.599708E-4 94.995094 8 TACCTGG 50 6.366463E-11 76.07904 2 ACCTGGG 50 6.366463E-11 76.03754 3 TATTGTC 20 0.0020594609 71.324104 2 GTCTTGG 20 0.002063927 71.28519 6 TCTTGGG 20 0.002063927 71.28519 7 GGGAGTC 20 0.0020684004 71.24632 9 GGGTGGC 20 0.0020684004 71.24632 8 AGGCTTA 65 9.094947E-12 65.80171 6 TATAGGC 65 9.094947E-12 65.80171 3 ATATAGG 70 1.8189894E-11 61.13494 2 GATATAG 80 1.8189894E-12 59.436752 1 TACAGGG 25 0.004986019 57.05928 2 CTGAGTG 25 0.0049968087 57.02815 7 GCGTGCA 25 0.0050076162 56.997055 9 CTTACTA 85 1.2551027E-10 50.29152 9 AAGTACT 40 5.334684E-4 47.5494 2 >>END_MODULE