FastQCFastQC Report
Fri 10 Feb 2017
SRR2568696_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568696_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541132
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26350.034.2519071
TCAACGC28650.030.6725834
CAACGCA30450.029.015425
ATCAACG30350.028.7980023
GGTATCA18850.028.7279931
AACGCAG31050.028.6077216
ACATGGG67450.028.5921843
GTACATG73250.028.0796741
TACATGG75300.026.1171462
CATGGGG38550.025.0135234
TATCAAC37800.023.3744532
ACGCAGA37800.023.3735337
CGTATAC1652.4313395E-623.0308153
CGCAGAG38250.022.9743658
ATGGGGG19750.020.9245155
ATACCGG1404.8532363E-420.3575946
ATAGGAC7550.019.5037443
TATAATA17800.018.1471842
GTATATA20700.018.1287781
GAGTACA49150.017.8796881
Produced by FastQC (version 0.11.5)