FastQCFastQC Report
Fri 10 Feb 2017
SRR2568696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2541132
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC27240.1071963203800511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29900.033.5756651
TCAACGC30150.032.307754
CGAAACG300.003953331.66488868-69
ATCAACG31400.030.870293
CAACGCA31600.030.8252775
AACGCAG32800.029.8423866
GGTATCA19000.028.547271
ACATGGG61650.026.7446293
ACGCAGA38200.025.4984387
GTACATG71450.025.370911
CATGGGG35700.024.4900554
CGCAGAG39650.024.437478
TATCAAC42200.023.785172
TACATGG74350.023.2893142
AGAGTAC46550.017.55003510-11
TAATACT31600.017.2922294
ATAGGAC7500.017.1057723
GCAGAGT57450.017.0312469
CATGGGA25800.016.7595314
GAGTACA47250.016.1113341
Produced by FastQC (version 0.11.5)