FastQCFastQC Report
Fri 10 Feb 2017
SRR2568693_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568693_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064124
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27050.035.6473581
GTATCAA41100.034.5563621
TCAACGC44650.030.637154
ATCAACG45650.029.9660193
AACGCAG46750.029.3630186
CAACGCA47750.028.5491355
GTACATG89050.025.8705961
TATCAAC55300.025.0816632
ACATGGG83850.024.8678843
ACGCAGA55450.024.6703537
TACATGG92300.024.0847852
CGCAGAG56650.023.896238
CATGGGG47000.021.626854
TACCTGG21000.018.5478322
GTATATA31550.018.5184381
GAGTACA62100.018.4341891
GTACCTG22950.018.2137171
CATGGGA36800.017.9409054
ACCGTGC4900.017.4486128
GCAGAGT75950.017.3860729
Produced by FastQC (version 0.11.5)