FastQCFastQC Report
Fri 10 Feb 2017
SRR2568693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3064124
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC33020.10776326284445407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41000.035.2700771
TCAACGC42600.032.1236084
GGTATCA25300.031.2107561
ATCAACG43950.031.028763
CAACGCA45550.030.1474725
AACGCAG46300.029.9670036
TATCAAC54600.025.5206832
ACGCAGA54850.025.2945187
CGCAGAG55450.024.9265828
GTACATG84200.024.1795581
ACATGGG76700.024.0368653
TACATGG86050.022.7147672
GTATATA29950.021.7590751
CATGGGG41350.019.8797994
GCAGAGT71150.019.2259989
CATGGGA34750.018.1860454
AGAGTAC63650.018.02146310-11
TAATACT34000.017.8884414
GCGGTCG1350.0094066517.591699
GTATTAT19950.017.1674691
Produced by FastQC (version 0.11.5)