FastQCFastQC Report
Fri 10 Feb 2017
SRR2568692_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568692_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195500
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12950.033.7446981
GTACATG34350.032.357621
CAACGCA13000.032.1533935
TCAACGC13050.031.666224
TACATGG34600.031.3001372
ACATGGG34750.030.3436263
GGTATCA8500.029.6172311
TCGCACT650.00584456829.2303564
ATCAACG14300.028.8969883
AACGCAG15050.027.7736956
GCACCGT1955.347829E-1026.7944956
TATCAAC16450.025.6987252
CATGGGG24600.025.6805324
TACCCCG1201.7110351E-423.7496665
CCGTTCA1452.3346991E-522.9307129
ACCGTGC2503.8380676E-1022.7996798
GACCGTA1050.002196452322.6187297
ACGCAGA18300.022.5816487
GGACCGT1503.0336749E-522.1663536
CCGTATA1302.9418844E-421.9227689
Produced by FastQC (version 0.11.5)