FastQCFastQC Report
Fri 10 Feb 2017
SRR2568692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195500
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12250.034.161281
TCAACGC12900.030.199084
CAACGCA13300.029.648045
GTACATG33350.028.3755841
ATCAACG13750.028.3322263
ACATGGG32650.027.209963
AACGCAG15050.026.2005946
TACATGG35600.025.3757252
TATCAAC15900.025.1186922
CATGGGG21550.023.5888124
GAGTACA22450.021.8176751
ACGGCGT1100.002878006321.5901688
TATACCG1353.7915897E-421.1148015
ACGCAGA18000.020.8508667
CGCAGAG18050.020.525618
CTAGGAC5300.019.7204283
AGTACAT23700.019.660412
GGTATCA8600.019.3533951
GTATAAG4750.019.0216241
GTATTAT4001.8189894E-1219.0216221
Produced by FastQC (version 0.11.5)