FastQCFastQC Report
Fri 10 Feb 2017
SRR2568691_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568691_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239655
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC21300.034.343374
ATCAACG21750.034.2880023
GTATCAA24250.033.302571
CAACGCA22300.033.0163155
AACGCAG23600.031.197626
GGTATCA15650.028.2298871
TATCAAC28300.027.6967222
GTACATG55050.027.6142481
ACATGGG52700.025.9587423
ACGCAGA28600.025.9095767
TACATGG56900.025.3800332
CGCAGAG30250.024.0252468
TTATCGT1401.7802007E-523.7504444
CATGGGG32150.022.014414
GAGTACA40900.019.5131131
AGTACAT41500.019.1160982
GTATATA13050.018.9292491
GTGTAGC14600.018.5465241
TAATACT14500.017.3623944
TAGACCG1650.001464214617.2730525
Produced by FastQC (version 0.11.5)