FastQCFastQC Report
Fri 10 Feb 2017
SRR2568691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239655
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT32350.14444188948744338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19400.032.084614
CAACGCA20450.030.669585
ATCAACG20350.030.5868033
GTATCAA23350.029.9402561
AACGCAG21600.029.036716
GGTATCA14000.027.5157951
ACATGGG43800.026.577813
TATCAAC24950.026.1129342
GTACATG48050.025.7338641
ACGCAGA25700.024.217897
TACATGG50000.023.2073462
CGCAGAG27500.022.2807128
CATGGGG27550.021.2134234
GGGCCGT1856.5392396E-620.5468626
AATACCG2551.1890734E-820.4965025
TGTACGC1404.815276E-420.3811592
TATACTG14150.019.4759565
CCGTGCG1559.6316135E-418.3861859
GAGTACA34950.018.234031
GTATATA13700.017.7041341
Produced by FastQC (version 0.11.5)