FastQCFastQC Report
Fri 10 Feb 2017
SRR2568690_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568690_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948608
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31840.16339869281045752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.11438934870430584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.049.389821
GTATCAA41900.043.3039361
TCAACGC50850.034.8414234
ATCAACG52000.034.0708923
CAACGCA53150.033.423075
AACGCAG54450.032.6259276
TATCAAC55950.032.0051042
ACGCAGA61450.028.6001937
CGCAGAG63150.027.529418
GTACATG61250.025.7460141
ACATGGG60500.023.78843
CGTATAC1801.953158E-723.7491473
TACATGG64150.023.6195722
GCAGAGT72800.023.5540169
AGAGTAC65800.020.28448510-11
GTATTAT13100.019.9420321
GAGTACT39150.019.65396112-13
CATGGGG38700.019.6375374
CAGAGTA71900.018.82779910-11
GTGGTAT15200.018.7493271
Produced by FastQC (version 0.11.5)