FastQCFastQC Report
Fri 10 Feb 2017
SRR2568690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948608
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39360.20199034387624396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.1422040759352317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.050.2866551
GTATCAA43350.043.563271
TCAACGC51700.036.303294
ATCAACG52950.035.3565333
CAACGCA53600.035.105075
AACGCAG54650.034.430596
TATCAAC56350.033.4227522
CGCGAAT452.4152447E-531.6651178-79
ACGCAGA63150.029.4185057
CGCAGAG63400.028.9930558
GCAGAGT76950.023.4556379
GTACATG58950.023.1588611
TACATGG59550.022.7616672
ACATGGG56600.021.9950163
AGAGTAC67100.020.77580510-11
GAGTACT45100.020.64212-13
CAGAGTA73550.020.27772510-11
GTACCGG2001.269616E-519.0273841
TATACAG18450.018.800355
GTGTAGC11600.018.4532831
Produced by FastQC (version 0.11.5)