FastQCFastQC Report
Fri 10 Feb 2017
SRR2568688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769234
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36100.033.421711
TCAACGC36750.031.4076424
CAACGCA38000.030.6244965
ATCAACG38600.029.7793033
GGTATCA25450.029.4897731
AACGCAG41100.028.6618446
GTACATG80500.027.8514771
TATCAAC45300.025.7952062
TACATGG86250.025.0584622
ACATGGG82500.025.0450423
ACGCAGA47950.024.1710477
CGCAGAG50650.022.8825638
CATGGGG57050.020.6482124
GTATAGG12350.020.3850141
ACCGTAT2603.2841854E-718.2692588
GAGTACA60600.018.0284041
GTATAGC11000.017.7048931
TACACCG3501.3133103E-917.6425655
TATAGCG1350.00940454317.5923025
GCAGAGT64600.017.2794389
Produced by FastQC (version 0.11.5)