FastQCFastQC Report
Fri 10 Feb 2017
SRR2568688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2769234
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38550.036.3994371
TCAACGC40750.033.1126024
CAACGCA42050.032.3148845
ATCAACG41950.032.2786563
AACGCAG44450.030.7838766
GGTATCA25950.029.1445141
TATCAAC46800.029.0644572
ACGCAGA51600.026.1490277
CGCAGAG53750.025.0962688
GTACATG74100.023.8151191
GTATATA16400.022.6228751
ACATGGG75250.021.467183
TACATGG79400.021.3241422
CATGGGG47900.018.6476864
GCAGAGT70600.018.5683659
GGCGTAA1801.2317457E-418.4768586
TAGTCCG1300.007554808618.2738155
AATACCG2152.391241E-517.6788525
GTATAGC10300.017.0868321
AGAGTAC60550.016.47306410-11
Produced by FastQC (version 0.11.5)