##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568687_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 158997 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18939351056938 33.0 31.0 34.0 23.0 34.0 2 30.207532217589012 33.0 31.0 34.0 23.0 34.0 3 30.236381818524876 33.0 31.0 34.0 22.0 34.0 4 33.39052309163066 37.0 35.0 37.0 25.0 37.0 5 33.47076988874004 37.0 35.0 37.0 24.0 37.0 6 33.76806480625421 37.0 35.0 37.0 25.0 37.0 7 33.760530072894454 37.0 35.0 37.0 25.0 37.0 8 33.788065183619814 37.0 35.0 37.0 25.0 37.0 9 35.387296615659416 39.0 37.0 39.0 26.0 39.0 10-11 35.34224859588545 39.0 37.0 39.0 25.0 39.0 12-13 35.327147682031736 39.0 37.0 39.0 25.0 39.0 14-15 36.2738825260854 40.0 37.0 41.0 25.0 41.0 16-17 36.34432725145757 40.0 37.5 41.0 24.5 41.0 18-19 36.509600810078176 40.0 37.5 41.0 25.0 41.0 20-21 36.539576218419214 40.0 37.5 41.0 25.0 41.0 22-23 36.39991320590954 40.0 37.0 41.0 24.0 41.0 24-25 36.19587790964609 40.0 36.0 41.0 23.0 41.0 26-27 35.944948646829815 40.0 35.5 41.0 22.5 41.0 28-29 35.76017157556432 40.0 35.0 41.0 21.0 41.0 30-31 35.39101995635137 39.5 35.0 41.0 19.0 41.0 32-33 34.842795776020935 39.0 35.0 41.0 16.5 41.0 34-35 34.4125329408731 38.5 35.0 41.0 15.0 41.0 36-37 33.99610055535639 38.5 34.5 41.0 13.0 41.0 38-39 33.600794354610464 38.0 34.0 41.0 11.0 41.0 40-41 33.25652056328107 38.0 33.0 41.0 9.0 41.0 42-43 32.67694673484405 38.0 32.5 41.0 8.0 41.0 44-45 31.947187682786467 37.0 30.0 40.0 8.0 41.0 46-47 31.292769674899528 37.0 26.0 40.0 7.5 41.0 48-49 30.660575356767737 37.0 22.5 40.5 7.0 41.0 50-51 29.357475298276068 36.0 19.0 39.0 7.0 40.0 52-53 29.351981483927368 35.5 17.5 39.5 7.0 40.5 54-55 29.553831204362346 36.5 15.0 40.0 7.0 41.0 56-57 29.15140222771499 35.5 13.5 40.0 7.0 41.0 58-59 28.7019377724108 35.5 11.5 40.0 7.0 41.0 60-61 28.042359289797922 35.0 10.0 40.0 7.0 41.0 62-63 27.70003207607691 35.0 10.0 40.0 7.0 41.0 64-65 27.423787870211388 35.0 10.0 39.5 7.0 41.0 66-67 26.98924193538243 35.0 10.0 39.0 7.0 41.0 68-69 26.441564935187458 34.0 10.0 38.5 7.0 40.5 70-71 25.805776209614017 33.5 9.0 37.0 7.0 39.5 72-73 25.265398089272125 32.5 8.0 37.0 7.0 39.0 74-75 24.893196098039585 32.5 8.0 36.0 7.0 39.0 76-77 24.52322685333686 32.0 8.0 35.5 7.0 37.5 78-79 24.155078397705616 31.5 8.0 35.0 7.0 37.0 80-81 23.764262847726688 31.0 8.0 35.0 7.0 36.5 82-83 23.392394196116907 30.0 7.0 35.0 7.0 36.0 84-85 23.08485694698642 29.0 7.0 35.0 7.0 36.0 86-87 23.022340044151775 29.5 7.0 35.0 7.0 35.5 88-89 22.7241111467512 29.0 7.0 35.0 7.0 35.0 90-91 22.455646961892363 29.0 7.0 35.0 7.0 35.0 92-93 22.238482487090952 27.0 7.0 35.0 7.0 35.0 94-95 22.02115448719158 26.5 7.0 35.0 6.5 35.0 96-97 21.79807480644289 25.0 7.0 35.0 5.0 35.0 98-99 21.686884029258415 25.0 7.0 35.0 5.0 35.0 100-101 20.605190663974792 22.0 7.0 34.0 5.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.33727693724762275 1101 2 -0.17664187323815028 1101 3 0.2590754631064591 1101 4 -0.6689023218510783 1101 5 -0.46422679922869037 1101 6 -0.2113797712251113 1101 7 -0.3284762276285136 1101 8 -0.14307918327148172 1101 9 0.03395103487414275 1101 10-11 0.30306473918379595 1101 12-13 0.34454617134972665 1101 14-15 0.30030887213058577 1101 16-17 -0.0172073695085615 1101 18-19 0.07806048211622141 1101 20-21 -0.18969250308572327 1101 22-23 0.16320992609691132 1101 24-25 0.40019880133948504 1101 26-27 0.011440839635582734 1101 28-29 0.3290862036554998 1101 30-31 0.73780344455772 1101 32-33 0.7535358914566217 1101 34-35 0.7405147141894801 1101 36-37 -0.054120692623222055 1101 38-39 -0.10379758595612998 1101 40-41 0.1635165475934386 1101 42-43 0.29827831221221146 1101 44-45 0.6058613597550568 1101 46-47 0.21976713394588643 1101 48-49 0.08722529509738663 1101 50-51 -0.164261913449554 1101 52-53 0.18564611759783034 1101 54-55 0.6215990283653703 1101 56-57 0.1074459270246173 1101 58-59 -0.25227055470389814 1101 60-61 -0.041872063014210426 1101 62-63 -0.6111236716381718 1101 64-65 -1.0618573775553841 1101 66-67 -1.300339066055713 1101 68-69 -1.1896819682340727 1101 70-71 -0.6353850908905976 1101 72-73 -1.2995477931661306 1101 74-75 -1.1509226236590386 1101 76-77 -1.6426028338398417 1101 78-79 -1.6306009639199992 1101 80-81 -1.1770980573958951 1101 82-83 -1.1410852798993965 1101 84-85 -0.6606970833325114 1101 86-87 -0.8497246975549508 1101 88-89 -1.4979355935633514 1101 90-91 -1.7644068077283457 1101 92-93 -1.1329754287420606 1101 94-95 -0.9530239252964279 1101 96-97 -1.2227068587555934 1101 98-99 -0.8932761117974657 1101 100-101 -0.6281608665022631 1102 1 0.40279783576288253 1102 2 0.48112324455901145 1102 3 0.3747955984100031 1102 4 0.4421296643811985 1102 5 0.5885297137378984 1102 6 0.18551062103647098 1102 7 0.3680402995805565 1102 8 0.20568350143565794 1102 9 0.7133765882537659 1102 10-11 0.4345109441567985 1102 12-13 0.37579060788168306 1102 14-15 0.6541454396933446 1102 16-17 0.36843739934789 1102 18-19 0.22775486227170205 1102 20-21 0.39054012405806304 1102 22-23 0.6820218660411328 1102 24-25 0.8195507685817915 1102 26-27 0.6808966582813625 1102 28-29 0.9078649111506039 1102 30-31 0.6987849886797335 1102 32-33 1.1655885886259298 1102 34-35 1.5436421250256345 1102 36-37 1.8273403482645563 1102 38-39 2.080583162960849 1102 40-41 2.021180781170081 1102 42-43 1.88402989965509 1102 44-45 2.855846523840274 1102 46-47 2.6632719704541046 1102 48-49 2.398304756256959 1102 50-51 2.520830959376987 1102 52-53 2.2945417321014183 1102 54-55 2.6243881803444857 1102 56-57 2.6161101012576005 1102 58-59 2.2718823145620206 1102 60-61 1.7968021996408297 1102 62-63 2.0253964161904463 1102 64-65 1.6364097875980192 1102 66-67 1.9230898105688183 1102 68-69 2.2389572816620777 1102 70-71 2.3282669178922646 1102 72-73 2.3335897060920736 1102 74-75 2.136449045678088 1102 76-77 2.333451999701193 1102 78-79 2.273766729391969 1102 80-81 2.3117750065172267 1102 82-83 1.609879054561464 1102 84-85 1.765924482153217 1102 86-87 1.7250779847784443 1102 88-89 1.8604407639228526 1102 90-91 2.1741131614129507 1102 92-93 2.0138407839456143 1102 94-95 2.665337002838399 1102 96-97 1.5144304031279745 1102 98-99 2.10138295888083 1102 100-101 2.1928586575615903 1103 1 -0.25225951564483395 1103 2 -0.11136893479448062 1103 3 -0.21566321285172663 1103 4 0.017624971993292604 1103 5 0.3306047919443671 1103 6 0.10819049590612906 1103 7 0.34457836006021836 1103 8 -0.2301871972087639 1103 9 -0.6816703356252773 1103 10-11 -0.35349739786614265 1103 12-13 -0.381847556873268 1103 14-15 -0.7478618595767301 1103 16-17 -0.2683978456990346 1103 18-19 -0.22493022636228943 1103 20-21 -0.4295589374643498 1103 22-23 -0.5598446615918178 1103 24-25 -0.17828551921800084 1103 26-27 -0.6553129350450178 1103 28-29 -0.8821507301424134 1103 30-31 -0.43330573081974677 1103 32-33 -0.8820912862437282 1103 34-35 -0.089595622628174 1103 36-37 -0.14625193536421932 1103 38-39 -0.33878597155426604 1103 40-41 -0.31148345240656283 1103 42-43 -0.6054382963824523 1103 44-45 -0.18714096312531936 1103 46-47 0.0419216054906002 1103 48-49 0.22778859358751546 1103 50-51 0.8862897706387152 1103 52-53 0.7976960595258191 1103 54-55 0.4024643013038123 1103 56-57 0.6386596320188112 1103 58-59 0.344796808826878 1103 60-61 0.5472975072529245 1103 62-63 0.3355371878275655 1103 64-65 0.4640427385886383 1103 66-67 0.6360720837700704 1103 68-69 0.5048592628925235 1103 70-71 0.2839238521988321 1103 72-73 0.38544059243201545 1103 74-75 -0.20307128800282825 1103 76-77 -0.35906624847399016 1103 78-79 -0.5950028222242949 1103 80-81 -0.35741164624606725 1103 82-83 -0.24593429267524058 1103 84-85 -0.18590033536503014 1103 86-87 0.38227297956467865 1103 88-89 -0.6445383810197995 1103 90-91 -0.17317568113136517 1103 92-93 -0.3248453474412507 1103 94-95 0.005222300022964532 1103 96-97 -0.30214936746639864 1103 98-99 -0.5419694915884072 1103 100-101 -0.14032695244883442 1104 1 -0.3367579958879965 1104 2 -0.3387245578947855 1104 3 -0.0451971540877949 1104 4 -0.23333754067135004 1104 5 -0.14300209761996996 1104 6 -0.2951023105781658 1104 7 -0.22330411207757095 1104 8 -0.07821151331818754 1104 9 0.22820808382761015 1104 10-11 -0.027154945991782142 1104 12-13 -0.2040613360019421 1104 14-15 -0.20193987376112688 1104 16-17 -0.5172073695085615 1104 18-19 -0.11740743000971321 1104 20-21 -0.5474160803214971 1104 22-23 -0.6795508419362974 1104 24-25 -0.9305398251957087 1104 26-27 -0.5798063494320473 1104 28-29 -0.0020848740127306087 1104 30-31 0.3040549581366818 1104 32-33 0.10010222946042546 1104 34-35 -0.42943858108815647 1104 36-37 -0.7027109019295708 1104 38-39 -0.6850119087376498 1104 40-41 -0.042157211271806716 1104 42-43 0.13437933279688252 1104 44-45 0.38877089097802653 1104 46-47 0.8796875629374057 1104 48-49 0.7919476614699867 1104 50-51 0.5684832863428717 1104 52-53 0.7998997069422309 1104 54-55 0.7573021939076661 1104 56-57 0.7996272105395796 1104 58-59 0.7880338908937503 1104 60-61 0.709987476857787 1104 62-63 0.5199090216674875 1104 64-65 0.08197586928772438 1104 66-67 0.06793631882579376 1104 68-69 0.537787327735483 1104 70-71 0.33339193730521544 1104 72-73 0.45187929557284434 1104 74-75 0.49784056610051763 1104 76-77 0.12231673024941259 1104 78-79 0.0716131858810698 1104 80-81 0.23909291302444657 1104 82-83 -0.001836015066324137 1104 84-85 -0.24476558359198108 1104 86-87 -0.10582225853055505 1104 88-89 0.028789886457406766 1104 90-91 0.9353856157278067 1104 92-93 0.9744454327005947 1104 94-95 1.1755870416643042 1104 96-97 0.8683673498588291 1104 98-99 1.415148036273802 1104 100-101 0.3144096230324216 1105 1 -0.9029793384576799 1105 2 -0.8816900865109822 1105 3 -0.7846000899281407 1105 4 -0.5231280734109163 1105 5 -0.7192844660844315 1105 6 -0.46152157496873514 1105 7 -0.9206486609807527 1105 8 -0.8713832104977968 1105 9 -1.070983735403793 1105 10-11 -0.9012271874563993 1105 12-13 -0.9690291737060477 1105 14-15 -0.5519316270185897 1105 16-17 -0.9939515555550642 1105 18-19 -1.2657663282449008 1105 20-21 -0.8071060028021151 1105 22-23 -0.612834694814417 1105 24-25 -0.40118142176506666 1105 26-27 -0.14454327834512526 1105 28-29 -0.37038439570165593 1105 30-31 -0.8879292081176473 1105 32-33 -0.644743556454138 1105 34-35 -0.14311168021399823 1105 36-37 -0.4402995544118369 1105 38-39 0.0022716142707608356 1105 40-41 -0.04966174698020609 1105 42-43 0.7669869527869864 1105 44-45 0.760616511957739 1105 46-47 0.3568440861107547 1105 48-49 0.5145272425354648 1105 50-51 0.31588556244381394 1105 52-53 -0.04275244545756962 1105 54-55 -0.8522145469796847 1105 56-57 -1.3121986182580443 1105 58-59 -0.7091622166437723 1105 60-61 -0.9320214296241502 1105 62-63 -0.8424528453950231 1105 64-65 -0.5973083134600898 1105 66-67 -0.1687451919774361 1105 68-69 -0.25538436944413334 1105 70-71 -0.21394436485542911 1105 72-73 -0.6498030399046435 1105 74-75 -0.44039133229961536 1105 76-77 -0.13135307017941855 1105 78-79 -0.05951002045242504 1105 80-81 0.513297102369652 1105 82-83 0.32696160987182665 1105 84-85 0.281541525100085 1105 86-87 0.3515420825547153 1105 88-89 0.21335198442870862 1105 90-91 0.3122617496549047 1105 92-93 0.42166628046572896 1105 94-95 -0.14001579521512753 1105 96-97 0.16706436453803164 1105 98-99 0.055012789696199604 1105 100-101 0.3147394927338887 1106 1 0.3131844997991813 1106 2 0.07473145130590453 1106 3 0.022753775565259815 1106 4 0.07785663222495032 1106 5 0.01824957958915263 1106 6 0.12903991675555204 1106 7 -0.12549886001699662 1106 8 0.4551409881194246 1106 9 0.030105726150786438 1106 10-11 0.27565318128443295 1106 12-13 0.003577153551439949 1106 14-15 0.686597599882731 1106 16-17 0.5109457836445941 1106 18-19 0.32091919448713213 1106 20-21 0.1992055413001239 1106 22-23 0.4940163422691839 1106 24-25 0.5173708514383719 1106 26-27 0.13300752827544216 1106 28-29 -0.1307028536945367 1106 30-31 -0.3945026420166542 1106 32-33 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1.5197100450911307 1106 100-101 1.4505031633812813 1107 1 0.10319161217471517 1107 2 -0.030353897200495084 1107 3 -0.16679103240059945 1107 4 -0.11808931372099352 1107 5 -0.17165084715337997 1107 6 -0.16398995522168747 1107 7 0.1284129465263888 1107 8 0.2641737050468791 1107 9 0.3677657546002848 1107 10-11 0.11473568484062469 1107 12-13 0.46232537545756003 1107 14-15 0.11529603516011377 1107 16-17 0.23498561294758247 1107 18-19 -0.13000541433221713 1107 20-21 0.10389970915218782 1107 22-23 -0.03710030068956627 1107 24-25 0.08280470634591097 1107 26-27 -0.15362120572170568 1107 28-29 -0.07247027901458836 1107 30-31 0.16941983309002495 1107 32-33 0.06114179646304052 1107 34-35 0.09123144504099656 1107 36-37 -0.04117674739429589 1107 38-39 0.11967267506227586 1107 40-41 -0.19832555766971893 1107 42-43 0.031215838955894526 1107 44-45 0.31577257070926734 1107 46-47 -0.004131026088344925 1107 48-49 -0.16328283498391372 1107 50-51 -0.45074030454925307 1107 52-53 -0.40803702318094537 1107 54-55 -0.35815599944806564 1107 56-57 0.2035092560789593 1107 58-59 -0.4087746197445483 1107 60-61 -0.24696941004031103 1107 62-63 0.15104470662456038 1107 64-65 -0.48633320126098667 1107 66-67 -0.5204128786359448 1107 68-69 -0.2141256995134917 1107 70-71 -0.28712877938011516 1107 72-73 -0.20393910681610805 1107 74-75 -0.26265775416824155 1107 76-77 -0.3853820379476751 1107 78-79 -0.4968825214724184 1107 80-81 -0.6252687891032132 1107 82-83 -1.3642613603444111 1107 84-85 -1.943137177470291 1107 86-87 -1.3667871708112607 1107 88-89 -1.4947263509446103 1107 90-91 -1.046953876620087 1107 92-93 -1.4801085053359841 1107 94-95 -0.9569017601274084 1107 96-97 -1.775739593030309 1107 98-99 -1.687082273543293 1107 100-101 -1.640890862223273 1108 1 0.7001214367361186 1108 2 0.2860336626081157 1108 3 0.5230813758928612 1108 4 0.5773652317335518 1108 5 0.41263942397899456 1108 6 0.2014805391961758 1108 7 0.600422515904377 1108 8 0.4408084737869089 1108 9 0.017247413292473368 1108 10-11 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-0.6830790164620204 2316 54-55 -0.6015630543192891 2316 56-57 -0.7776777539994306 2316 58-59 0.03271474195758017 2316 60-61 0.37168960451736055 2316 62-63 0.1574976741497558 2316 64-65 0.1862311884366612 2316 66-67 0.1693547585421058 2316 68-69 0.5945249163879645 2316 70-71 0.31637066070947384 2316 72-73 -0.10414907322148181 2316 74-75 -0.10215943389948379 2316 76-77 -0.030874759112286654 2316 78-79 0.3127479376541231 2316 80-81 0.2795184449393382 2316 82-83 0.3910717863521178 2316 84-85 -0.060368420471412776 2316 86-87 0.003397599625468928 2316 88-89 0.2521941417765561 2316 90-91 -0.03624558994595617 2316 92-93 -0.20995173041997006 2316 94-95 -0.16271083067612224 2316 96-97 -0.3209943522688299 2316 98-99 -0.15648316939995865 2316 100-101 -0.09268257554914072 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 17.0 7 650.0 8 1816.0 9 1571.0 10 1681.0 11 1988.0 12 2099.0 13 2021.0 14 1660.0 15 1658.0 16 2332.0 17 3335.0 18 3573.0 19 4189.0 20 5629.0 21 6787.0 22 4918.0 23 4325.0 24 4353.0 25 3300.0 26 2925.0 27 2730.0 28 2715.0 29 2297.0 30 2261.0 31 2605.0 32 3081.0 33 3969.0 34 5513.0 35 8233.0 36 12971.0 37 21622.0 38 23279.0 39 6889.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.99285462688069 18.78490917325014 13.309515422935641 13.91272077693353 2 13.850256952176073 28.58113335723586 36.85769818655294 20.710911504035124 3 26.603981257272242 33.721815151419854 27.354948268813484 12.319255322494419 4 11.307131581099014 18.690918696579182 38.4919212312182 31.510028491103604 5 9.33712395197152 44.332626785500885 30.962519419582485 15.36772984294511 6 22.71603695853172 37.63971092339722 18.555371755278667 21.088880362792395 7 22.174000553500896 38.2808765441417 22.01361109014517 17.53151181221224 8 26.486238301298176 26.587501257924927 21.16836067223508 25.757899768541815 9 28.60188947454493 19.650786861736254 22.40574641792359 29.341577245795225 10-11 24.136640532372244 28.641740021951552 23.66144295270353 23.560176492972673 12-13 32.042492704035425 27.56176411391768 24.0166171882862 16.37912599376069 14-15 26.7354101020774 26.69547224161462 31.36820191575942 15.200915740548565 16-17 17.73555475889482 31.397762221928716 31.74713988314245 19.119543136034014 18-19 17.85207757452255 31.780773669695055 33.82674352418779 16.540405231594605 20-21 20.83178716311834 26.870656309946856 32.40227680115727 19.89527972577754 22-23 18.017805535376787 29.2464252132922 31.042073517803964 21.69369573352705 24-25 21.18803972501934 30.45008270804375 29.411672211984175 18.950205354952736 26-27 20.240708476687068 26.182628986911045 34.985124757058664 18.591537779343227 28-29 17.178797742031858 33.70850826296838 32.50782269603912 16.604871298960646 30-31 19.182287080135865 31.013335010693172 30.83123663353881 18.973141275632155 32-33 17.523915545576333 32.17041830977943 33.11446127914363 17.191204865500605 34-35 18.139021490971526 31.61915004685623 32.72262998672931 17.51919847544293 36-37 17.869575745378043 31.21043544983695 34.60390637529757 16.31608242948744 38-39 18.039705276531244 31.928375781856833 32.51014959448793 17.521769347123993 40-41 16.81069454140644 34.20095976653648 31.14712856217413 17.841217129882953 42-43 16.557597179812134 34.41302167028834 28.843402213256265 20.18597893664326 44-45 18.15160034466059 30.661584809776286 30.051195934514485 21.13561891104864 46-47 19.232789174264035 32.88634927024401 30.82900658211288 17.05185497337908 48-49 17.696654831577987 31.64780285614549 31.546538944150754 19.10900336812577 50-51 13.252720296874019 32.00106924963834 35.3226618026291 19.423548650858542 52-53 15.706271187506681 34.11322226205526 27.96279175078775 22.217714799650306 54-55 14.421341283168864 30.765360352711056 29.238601986200997 25.574696377919082 56-57 18.432108781926704 26.830065976087596 30.00685547526054 24.73096976672516 58-59 21.841292603005087 26.103322704201965 28.762492374069954 23.292892318722995 60-61 18.19153820512337 27.329446467543413 32.495896148983945 21.983119178349277 62-63 15.953796853330482 26.972171818344776 32.43843301770198 24.63559831062276 64-65 15.320508587045056 30.451382009503774 30.747313501835027 23.480795901616144 66-67 18.451160450342787 25.239952198251466 29.195232404553746 27.113654946852 68-69 17.188836612489307 26.09067579127459 30.84901625320787 25.87147134302823 70-71 20.05044136191677 25.981703999773583 30.37645482762199 23.59139981068766 72-73 19.50162581683931 22.841940413970075 34.50002201299396 23.156411756196658 74-75 17.250325477839205 29.293005528406198 30.416926105524006 23.039742888230595 76-77 18.398774819650683 25.602055384692797 30.868821424303604 25.130348371352916 78-79 19.848173235973004 25.60960269690623 31.763177921596004 22.77904614552476 80-81 21.609780338675993 25.1965754975719 30.689366680924945 22.504277482827163 82-83 19.75077999194847 25.19436896135266 32.28977958937198 22.76507145732689 84-85 18.923653702201 28.6004541651727 29.99911934731055 22.476772785315745 86-87 19.53697267817997 29.006985353089 27.67564813446097 23.78039383427006 88-89 19.682339990564554 28.036483723855955 25.290139959113066 26.99103632646643 90-91 22.336249897783954 25.050164489202842 25.843046478421407 26.770539134591797 92-93 24.273579001330344 27.250340448416953 25.96780128505518 22.508279265197523 94-95 23.53457414181957 26.164870042244527 25.599932056456055 24.700623759479853 96-97 21.311305266733342 25.696475635146783 28.576101246076526 24.41611785204335 98-99 24.346841534179365 27.485335975216618 22.84285512100769 25.324967369596322 100-101 23.447731432848776 25.425375997785842 23.66694553300163 27.45994703636375 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 149.0 1 99.0 2 104.0 3 170.0 4 124.0 5 79.0 6 124.5 7 213.5 8 272.5 9 254.0 10 201.0 11 170.0 12 157.5 13 152.0 14 174.5 15 191.5 16 202.0 17 226.0 18 260.0 19 313.0 20 406.0 21 518.0 22 649.5 23 829.5 24 1047.5 25 1273.0 26 1622.5 27 2065.0 28 2670.0 29 3250.5 30 3664.5 31 4082.0 32 4720.0 33 5648.0 34 6044.0 35 5343.0 36 4673.0 37 4953.5 38 4551.0 39 3681.5 40 3083.0 41 2333.0 42 2063.5 43 2040.5 44 1998.5 45 2466.5 46 3614.5 47 8121.5 48 16865.0 49 17005.5 50 10907.0 51 7360.0 52 3913.5 53 2227.0 54 1532.0 55 1206.5 56 949.0 57 737.5 58 652.0 59 631.0 60 603.0 61 489.5 62 386.5 63 334.5 64 291.5 65 266.5 66 247.5 67 216.5 68 181.0 69 150.5 70 134.5 71 122.5 72 96.0 73 82.0 74 68.0 75 62.5 76 59.0 77 42.0 78 40.5 79 33.0 80 25.5 81 19.5 82 13.5 83 14.0 84 15.0 85 14.0 86 8.5 87 5.0 88 4.0 89 2.5 90 1.5 91 1.5 92 1.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008176254897891155 2 0.011320968320156985 3 0.0012578853689063315 4 0.0 5 0.005031541475625326 6 0.005031541475625326 7 0.0056604841600784925 8 0.003144713422265829 9 0.006918369528984823 10-11 0.00660389818675824 12-13 0.003144713422265829 14-15 0.0 16-17 0.0 18-19 9.434140266797486E-4 20-21 0.0012578853689063315 22-23 9.434140266797486E-4 24-25 0.002515770737812663 26-27 0.005031541475625326 28-29 0.0028302420800392462 30-31 0.010692025635703817 32-33 0.0 34-35 0.0 36-37 3.144713422265829E-4 38-39 3.144713422265829E-4 40-41 0.0 42-43 3.144713422265829E-4 44-45 0.0 46-47 0.0034591847644924113 48-49 0.004088127448945577 50-51 0.0044025987911721605 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0022012993955860803 64-65 0.004717070133398743 66-67 0.0044025987911721605 68-69 0.008176254897891155 70-71 3.144713422265829E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.015723567111329145 82-83 0.01320779637351648 84-85 0.015094624426875978 86-87 0.013522267715743063 88-89 0.013836739057969645 90-91 0.012578853689063316 92-93 0.00974861160902407 94-95 0.02610112140480638 96-97 0.011320968320156985 98-99 0.012264382346836734 100-101 0.012578853689063316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 158997.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.08873123392266 #Duplication Level Percentage of deduplicated Percentage of total 1 86.23387940048798 31.120712969427096 2 5.965493203206692 4.305741617766373 3 2.262112234227954 2.4491028132606276 4 1.15719762983618 1.6704717699076084 5 0.7302195887068665 1.3176349239293823 6 0.5106308818403624 1.1056812392686655 7 0.4304635761589404 1.0874419014195236 8 0.31195538515162075 0.9006459241369333 9 0.19518996165911467 0.6339742259287912 >10 1.7845939351690483 12.588287829330113 >50 0.19518996165911467 5.0309125329408735 >100 0.17079121645172535 12.652439983144337 >500 0.02614151272220286 6.377478820355101 >1k 0.024398745207389334 15.002169852261362 >5k 0.0017427675148135237 3.7573035969232125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5974 3.7573035969232125 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2845 1.7893419372692565 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2606 1.6390246356849503 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2411 1.5163808122165827 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1948 1.2251803493147668 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1676 1.0541079391435058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1650 1.0377554293477236 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1632 1.0264344610275664 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1621 1.019516091498582 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1447 0.9100800644037309 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1431 0.9000169814524802 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1302 0.8188833751580218 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1167 0.7339761127568445 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1075 0.6761133857871532 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1042 0.6553582772001987 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 983 0.618250658817462 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 953 0.599382378283867 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 943 0.5930929514393353 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 782 0.4918331792423757 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 737 0.4635307584419832 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 647 0.4069259168411983 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 641 0.40315226073447924 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 617 0.3880576363076033 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 599 0.3767366679874463 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 587 0.3691893557740083 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 570 0.3584973301383045 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 540 0.3396290496047095 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 524 0.3295659666534589 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 510 0.3207607690711145 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 507 0.31887394101775507 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 492 0.30943980075095756 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 490 0.30818191538205125 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 482 0.3031503739064259 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 472 0.29686094706189425 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 434 0.27296112505267395 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 406 0.2553507298879853 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 400 0.2515770737812663 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 395 0.2484323603590005 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 381 0.23962716277665616 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.23082196519431183 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 342 0.2150983980829827 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 334 0.21006685660735738 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 333 0.20943791392290417 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 328 0.20629320050063837 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 317 0.19937483097165354 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 305 0.19182751875821558 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 300 0.18868280533594972 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 289 0.1817644358069649 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 286 0.17987760775360542 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 286 0.17987760775360542 No Hit CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA 273 0.17170135285571425 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 268 0.16855663943344842 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 268 0.16855663943344842 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 264 0.16604086869563575 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 263 0.1654119260111826 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 258 0.16226721258891677 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 254 0.1597514418511041 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 252 0.1584935564821978 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 245 0.15409095769102563 No Hit GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT 243 0.1528330723221193 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 238 0.14968835889985344 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 224 0.14088316131750914 No Hit GATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCAT 224 0.14088316131750914 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 222 0.1396252759486028 No Hit AATATCTCCCATTCCCAAGTGTCAGAAGAAAGACATCTTTGCTCTTTCTA 218 0.13710950521079016 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 215 0.13522267715743064 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 208 0.13082007836625847 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 197 0.12390170883727367 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 191 0.12012805273055466 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.12012805273055466 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 188 0.11824122467719517 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 188 0.11824122467719517 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.117612281992742 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 187 0.117612281992742 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 186 0.11698333930828884 No Hit GATTTAAATGATGAGAAATTATTGGCTGGACACAAATTGCAGAAAAGTCA 184 0.11572545393938251 No Hit CATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTT 182 0.11446756857047619 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 181 0.113838625886023 No Hit ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT 181 0.113838625886023 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 180 0.11320968320156985 No Hit CTCCAAGCAATGGTTTGAGAAAGAATATGTGAAGAAAGCAAACAAAGTTT 171 0.10754919904149135 No Hit TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT 171 0.10754919904149135 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 168 0.10566237098813185 No Hit GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA 164 0.10314660025031919 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 164 0.10314660025031919 No Hit AAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 163 0.10251765756586603 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 162 0.10188871488141285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.289426844531658E-4 0.0 2 0.0 0.0 0.0 0.0018868280533594973 0.0 3 0.0 0.0 0.0 0.0018868280533594973 0.0 4 0.0 0.0 0.0 0.0018868280533594973 0.0 5 0.0 0.0 0.0 0.0018868280533594973 0.0 6 0.0 0.0 0.0 0.0018868280533594973 0.0 7 0.0 0.0 0.0 0.0018868280533594973 0.0 8 0.0 0.0 0.0 0.0018868280533594973 0.0 9 0.0 0.0 0.0 0.003144713422265829 0.0 10-11 0.0 0.0 0.0 0.0044025987911721605 0.0 12-13 0.0 0.0 0.0 0.006289426844531658 0.0 14-15 0.0 0.0 0.0 0.010377554293477236 0.0 16-17 0.0 0.0 0.0 0.015409095769102562 0.0 18-19 0.0 0.0 0.0 0.025157707378126632 0.0 20-21 0.0 0.0 0.0 0.03364843361824437 0.0 22-23 0.0 0.0 0.0 0.06352321112976975 0.0 24-25 0.0 0.0 0.0 0.11855569601942176 0.0 26-27 0.0 0.0 0.0 0.2132115700296232 0.0 28-29 0.0 0.0 0.0 0.3644722856406096 0.0 30-31 0.0 0.0 0.0 0.6063007478128518 0.0 32-33 0.0 0.0 0.0 0.9585086511066248 0.0 34-35 0.0 0.0 0.0 1.4714114102781815 0.0 36-37 0.0 0.0 0.0 2.058214934872985 0.0 38-39 0.0 0.0 0.0 2.757284728642679 0.0 40-41 0.0 0.0 0.0 3.778058705510167 0.0 42-43 0.0 0.0 0.0 4.428699912576967 0.0 44-45 0.0 0.0 0.0 4.922419919872702 0.0 46-47 0.0 0.0 0.0 5.472430297426995 0.0 48-49 0.0 0.0 0.0 5.983446228545192 0.0 50-51 0.0 0.0 0.0 6.5067265420102265 0.0 52-53 0.0 0.0 0.0 7.0567369195645195 0.0 54-55 0.0 0.0 0.0 7.535991245117833 0.0 56-57 0.0 0.0 0.0 8.008012729799933 0.0 58-59 0.0 0.0 0.0 8.380661270338434 0.0 60-61 0.0 0.0 0.0 8.700478625382868 0.0 62-63 0.0 0.0 0.0 9.028157763982968 0.0 64-65 0.0 0.0 0.0 9.366843399560999 0.0 66-67 0.0 0.0 0.0 9.733202513254966 0.0 68-69 0.0 0.0 0.0 10.19956351377699 0.0 70-71 0.0 0.0 0.0 10.702403189997295 0.0 72-73 0.0 0.0 0.0 11.12536714529205 0.0 74-75 0.0 0.0 0.0 11.534494361528834 0.0 76-77 0.0 0.0 0.0 11.95903067353472 0.0 78-79 0.0 0.0 0.0 12.481996515657528 0.0 80-81 0.0 0.0 0.0 13.039239734083033 0.0 82-83 0.0 0.0 0.0 13.51534934621408 0.0 84-85 0.0 0.0 0.0 13.999949684585243 0.0 86-87 0.0 0.0 0.0 14.485493436983088 0.0 88-89 0.0 0.0 0.0 15.06034705057328 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 60 0.0 79.17768 1 TATATTA 20 0.0020696367 71.25991 2 GTATATT 20 0.0020696367 71.25991 1 ATATTAT 20 0.0020696367 71.25991 3 ATATGTT 25 0.005010577 57.00793 2 TATCTTG 25 0.005010577 57.00793 7 GTTCAAT 25 0.005010577 57.00793 9 ATGTTCT 25 0.005010577 57.00793 4 AGTACTC 180 0.0 52.785122 5 AAAAAGT 175 0.0 51.5786 1 AGGATAT 95 7.2759576E-12 50.006954 8 AAGTACT 200 0.0 47.50661 4 CATATAG 100 1.2732926E-11 47.50661 3 TATATAT 40 5.370404E-4 47.50661 4 ATATATT 40 5.370404E-4 47.50661 5 GTATATA 40 5.370404E-4 47.50661 3 TAGGATA 100 1.2732926E-11 47.50661 7 ACATATA 100 1.2732926E-11 47.50661 2 GTACCCA 90 2.237357E-10 47.506607 1 AAAGTAC 205 0.0 46.347916 3 >>END_MODULE