##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568687_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 158997 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33859129417536 33.0 31.0 34.0 30.0 34.0 2 32.380145537337185 33.0 31.0 34.0 31.0 34.0 3 32.41925319345648 34.0 31.0 34.0 30.0 34.0 4 35.89917419825532 37.0 35.0 37.0 35.0 37.0 5 36.04566752831814 37.0 35.0 37.0 35.0 37.0 6 36.15292112429794 37.0 36.0 37.0 35.0 37.0 7 36.05416454398511 37.0 35.0 37.0 35.0 37.0 8 36.091901105052294 37.0 36.0 37.0 35.0 37.0 9 37.84465744636691 39.0 38.0 39.0 35.0 39.0 10-11 37.734205676836666 39.0 37.5 39.0 35.0 39.0 12-13 37.722205450417306 39.0 38.0 39.0 35.0 39.0 14-15 38.74852041233483 40.0 38.0 41.0 34.5 41.0 16-17 38.80267552217967 40.0 38.0 41.0 35.0 41.0 18-19 39.0870865488028 41.0 38.5 41.0 35.5 41.0 20-21 39.07106108920294 41.0 39.0 41.0 35.5 41.0 22-23 38.963081064422596 40.0 38.5 41.0 35.0 41.0 24-25 38.749136776165585 40.0 38.0 41.0 35.0 41.0 26-27 38.41221532481745 40.0 37.5 41.0 34.5 41.0 28-29 38.19951634307566 40.0 37.0 41.0 34.0 41.0 30-31 37.78214368824569 40.0 36.5 41.0 33.5 41.0 32-33 37.20545356201689 39.0 35.0 41.0 33.0 41.0 34-35 36.85933382390863 39.0 35.0 41.0 32.5 41.0 36-37 36.32863513148047 39.0 35.0 41.0 30.5 41.0 38-39 35.75357711151783 39.0 35.0 41.0 28.0 41.0 40-41 35.54493795480418 39.0 35.0 41.0 26.0 41.0 42-43 35.02567029566596 38.5 35.0 41.0 21.5 41.0 44-45 34.38982182053749 38.0 34.5 41.0 16.5 41.0 46-47 33.52397215041793 38.0 33.0 41.0 10.0 41.0 48-49 32.67352214192721 38.0 32.5 40.0 7.5 41.0 50-51 31.961537010132268 37.5 31.0 40.0 7.0 41.0 52-53 32.04890658314308 38.0 31.0 40.0 7.0 41.0 54-55 31.707906438486262 37.5 29.5 40.5 7.0 41.0 56-57 31.069221431850913 37.0 26.5 40.5 7.0 41.0 58-59 30.61491726258986 37.0 24.0 40.0 7.0 41.0 60-61 29.876066844028504 36.0 20.5 40.0 7.0 41.0 62-63 29.40974986949439 36.0 18.0 40.0 7.0 41.0 64-65 28.82908168078643 35.5 15.0 39.5 7.0 41.0 66-67 27.88355440668692 35.0 10.5 39.0 7.0 41.0 68-69 26.78807461776008 34.5 10.0 38.0 7.0 40.0 70-71 25.937813292074694 34.0 10.0 37.0 7.0 39.5 72-73 25.39035327710586 33.5 9.0 36.5 7.0 39.0 74-75 25.099879243004587 33.0 8.0 36.0 7.0 38.5 76-77 23.831047126675344 30.5 8.0 34.5 7.0 36.5 78-79 24.556645722875274 32.5 8.0 35.0 7.0 37.0 80-81 24.461408076881952 32.5 8.0 35.0 7.0 36.0 82-83 24.30385164499959 32.5 8.0 35.0 7.0 36.0 84-85 24.09408039145393 32.0 8.0 35.0 7.0 36.0 86-87 24.114753737491903 33.0 8.0 35.0 7.0 35.5 88-89 23.98358145122235 32.0 8.0 35.0 7.0 35.0 90-91 23.8565193053957 32.0 8.0 35.0 7.0 35.0 92-93 23.76308672490676 32.0 8.0 35.0 7.0 35.0 94-95 23.715947470706993 31.5 8.0 35.0 7.0 35.0 96-97 23.63234840908948 31.0 8.0 35.0 7.0 35.0 98-99 23.66908495128839 32.0 8.0 35.0 7.0 35.0 100-101 22.80196167223281 28.5 7.5 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1204439272620732 1101 2 0.3853053439203009 1101 3 0.06756578515257416 1101 4 0.26819909577388046 1101 5 0.27895588944841165 1101 6 0.19270395261668227 1101 7 0.25648368679485856 1101 8 0.2516374049441268 1101 9 0.03223952304512778 1101 10-11 0.3614953612444154 1101 12-13 0.4081003452509009 1101 14-15 0.049436221399467684 1101 16-17 -0.06123024517518161 1101 18-19 0.4845475524812457 1101 20-21 0.38255461125860535 1101 22-23 0.4463370120927479 1101 24-25 0.47730087614520755 1101 26-27 0.08127444881933599 1101 28-29 0.5971932314725237 1101 30-31 0.3415161437598684 1101 32-33 0.5127901129959511 1101 34-35 0.3535126220991742 1101 36-37 0.4897571642850167 1101 38-39 0.7275744466627287 1101 40-41 0.942022292626973 1101 42-43 0.48594015889014486 1101 44-45 0.41337519667447253 1101 46-47 0.08189973740361722 1101 48-49 -0.8020118796958897 1101 50-51 -1.186861763775152 1101 52-53 -0.5779185361007748 1101 54-55 -0.4169685257938873 1101 56-57 -0.5299820173012861 1101 58-59 0.16732621229312983 1101 60-61 0.20881689298317596 1101 62-63 0.386939571702861 1101 64-65 0.4543666005430289 1101 66-67 -0.14841630885327817 1101 68-69 -0.32068220100801526 1101 70-71 -0.4020615718129932 1101 72-73 -0.32424097990553236 1101 74-75 0.2062251116218654 1101 76-77 -0.054810967041110104 1101 78-79 0.0524255422614317 1101 80-81 -0.5067874242418497 1101 82-83 -0.7889392408499951 1101 84-85 -1.2474914538702784 1101 86-87 -0.8485233910237255 1101 88-89 -0.5923617062702036 1101 90-91 -0.9226076440432998 1101 92-93 -0.16944200198998516 1101 94-95 -1.0939024906340684 1101 96-97 -0.7578987212668657 1101 98-99 -0.3742623461903243 1101 100-101 -0.15533209748885568 1102 1 0.37550297420290235 1102 2 0.2446608717821519 1102 3 0.3720581001487133 1102 4 0.27264987020863174 1102 5 0.00318590547119868 1102 6 0.1269511783006152 1102 7 0.03744624094594684 1102 8 0.03657598425692754 1102 9 0.1273080649714231 1102 10-11 0.30191432747786706 1102 12-13 0.2560322187302191 1102 14-15 0.1211233616285341 1102 16-17 -0.04286338267444023 1102 18-19 0.23652072914731548 1102 20-21 -0.3116178414148223 1102 22-23 0.08030532257877354 1102 24-25 -0.019346207113947855 1102 26-27 0.1480360653406123 1102 28-29 0.45248371868395765 1102 30-31 0.7551414478961149 1102 32-33 0.7165465666188808 1102 34-35 1.1123399913947694 1102 36-37 0.6533379606769145 1102 38-39 0.2775714794797679 1102 40-41 0.26781898125079806 1102 42-43 0.7399903636257719 1102 44-45 1.0792110521133154 1102 46-47 1.9031269642744277 1102 48-49 2.1292384912019813 1102 50-51 1.9446437848569822 1102 52-53 1.8288525754059606 1102 54-55 1.7550093983649155 1102 56-57 1.7488738369506862 1102 58-59 2.0289367992019187 1102 60-61 2.4166680590860814 1102 62-63 1.8491376608370338 1102 64-65 1.2379996193549694 1102 66-67 0.8904537878768863 1102 68-69 0.917226528444246 1102 70-71 1.4320432290890324 1102 72-73 1.838746379895074 1102 74-75 2.0008070355432963 1102 76-77 1.888248792023635 1102 78-79 2.0991883456558895 1102 80-81 1.6587813847309079 1102 82-83 1.6248047506045147 1102 84-85 2.4420818652205796 1102 86-87 2.0947143990184074 1102 88-89 2.0738895547825535 1102 90-91 1.6567051563839748 1102 92-93 1.3711193890253845 1102 94-95 1.3260429131995295 1102 96-97 1.4777846209680163 1102 98-99 1.37735476852097 1102 100-101 1.9831760029206187 1103 1 0.03780745804023411 1103 2 -0.0338584191459006 1103 3 -0.09561656095660709 1103 4 -0.06681832582890479 1103 5 0.2732067605285522 1103 6 0.027837518238051473 1103 7 0.2630458238447986 1103 8 0.2042506453622437 1103 9 0.3411245404667298 1103 10-11 0.0713095308146805 1103 12-13 -0.14428060713004953 1103 14-15 0.0723746650696171 1103 16-17 -0.020783090703631046 1103 18-19 0.03242792414209816 1103 20-21 0.19672418152573812 1103 22-23 0.12159833613456072 1103 24-25 0.10654013282348984 1103 26-27 0.356013124777526 1103 28-29 0.3382501420416233 1103 30-31 0.3748494770931998 1103 32-33 -0.12187887655108653 1103 34-35 -0.13867669265111715 1103 36-37 0.10836924326294906 1103 38-39 0.7096882678009351 1103 40-41 1.187318459874362 1103 42-43 0.7720028766601743 1103 44-45 0.2647689248974672 1103 46-47 0.4396233146393911 1103 48-49 0.7645096069475485 1103 50-51 -0.4535574664464619 1103 52-53 -0.11880123064200632 1103 54-55 -0.12332741081131005 1103 56-57 0.18096455645016718 1103 58-59 -0.0190559015280094 1103 60-61 0.04993187556157608 1103 62-63 0.18844944394443885 1103 64-65 -0.10222457251155603 1103 66-67 -0.21447321942238418 1103 68-69 -0.389003919939487 1103 70-71 -0.18975030584319086 1103 72-73 -0.2864361018567507 1103 74-75 -0.5217946328612904 1103 76-77 -0.5798690390504007 1103 78-79 -0.697109881664236 1103 80-81 -0.3615783650084019 1103 82-83 -0.8544444673308327 1103 84-85 0.20047602580452306 1103 86-87 0.14244641153144144 1103 88-89 -0.5833315088253705 1103 90-91 -0.660760954147829 1103 92-93 -0.7332341041967219 1103 94-95 -0.5415796102624064 1103 96-97 -0.03371172939694844 1103 98-99 -0.9048662950869542 1103 100-101 -0.8177885434818855 1104 1 0.09155619157620265 1104 2 0.11943438733839429 1104 3 -0.0697806946952042 1104 4 0.11165178561992661 1104 5 0.2850102053107193 1104 6 0.2959732831823274 1104 7 0.2320934428924204 1104 8 0.1308970486043961 1104 9 0.013211673192159878 1104 10-11 0.02617275269917485 1104 12-13 0.08540530978991256 1104 14-15 0.2575317066096403 1104 16-17 0.22868499440275514 1104 18-19 0.16609053812386776 1104 20-21 0.3046269170880507 1104 22-23 0.06050411121054822 1104 24-25 -0.12337374762230979 1104 26-27 -0.008069955263003692 1104 28-29 0.32371113494942705 1104 30-31 0.09683529269603497 1104 32-33 0.23186985698487916 1104 34-35 0.4195046649983212 1104 36-37 0.3704006515709537 1104 38-39 0.35791521815554717 1104 40-41 0.252262735456938 1104 42-43 0.411212603103948 1104 44-45 0.10028057636656484 1104 46-47 -0.5000929974173474 1104 48-49 -0.7425066037920658 1104 50-51 -0.5636131908638866 1104 52-53 -0.16094915364099194 1104 54-55 0.3191497928361038 1104 56-57 0.2996322362880619 1104 58-59 0.1687100559187975 1104 60-61 0.38032701233969135 1104 62-63 0.4425527874094861 1104 64-65 0.17647350246311433 1104 66-67 0.043505503981872806 1104 68-69 0.3959758166359926 1104 70-71 0.5710399677130411 1104 72-73 0.46852843715034354 1104 74-75 0.3113107369462078 1104 76-77 -0.09554786377178104 1104 78-79 0.5201646877379922 1104 80-81 0.8044044110807569 1104 82-83 0.5825362824158766 1104 84-85 0.858632054173313 1104 86-87 0.279655124803984 1104 88-89 0.05960162187371765 1104 90-91 0.33098170036078045 1104 92-93 0.5899421875965913 1104 94-95 0.32280742114589955 1104 96-97 0.24625280961014795 1104 98-99 0.4198044242646155 1104 100-101 0.23603617789602893 1105 1 0.05823935172794137 1105 2 -0.3187975110617387 1105 3 0.12088399275324946 1105 4 0.2757619621002192 1105 5 -0.008243959294262027 1105 6 0.12180208080726374 1105 7 0.20108569095443585 1105 8 -0.17736618741737686 1105 9 0.1984335105663959 1105 10-11 0.036481180870048036 1105 12-13 -0.25469035242351623 1105 14-15 -0.21195534600457222 1105 16-17 0.04956574650566381 1105 18-19 0.06102703820633337 1105 20-21 0.4924052446486584 1105 22-23 0.30188626525970363 1105 24-25 0.25457446283456164 1105 26-27 0.17921356774540698 1105 28-29 -0.010199470361477836 1105 30-31 0.31050839182187673 1105 32-33 0.43886309465599993 1105 34-35 0.6656699297187672 1105 36-37 0.7679761092651631 1105 38-39 0.6678278026846627 1105 40-41 1.0227558906606333 1105 42-43 1.3153029874021485 1105 44-45 0.6804389138232736 1105 46-47 0.4745070355696299 1105 48-49 0.36049521049128685 1105 50-51 0.3696706620142258 1105 52-53 0.9150858125473675 1105 54-55 1.1341753577379698 1105 56-57 1.1107818321976737 1105 58-59 1.0295875093247062 1105 60-61 1.6589019752293517 1105 62-63 1.3232224229034664 1105 64-65 1.077924929149571 1105 66-67 0.6488988736008707 1105 68-69 0.3745342860405785 1105 70-71 -0.1935155328642324 1105 72-73 -0.22015703208931114 1105 74-75 -0.24773593961655038 1105 76-77 -0.0347693712763153 1105 78-79 -0.2745738905235946 1105 80-81 -0.1711852310106181 1105 82-83 0.5505389213734837 1105 84-85 0.3376853281301031 1105 86-87 0.3958517271460593 1105 88-89 0.023728291839081095 1105 90-91 -0.6002404668831574 1105 92-93 -0.42506688382019675 1105 94-95 -0.36843453828012684 1105 96-97 -0.15173498521090067 1105 98-99 -0.46738013783335575 1105 100-101 0.2986289537495672 1106 1 0.5285410487738247 1106 2 0.19529216000467642 1106 3 0.3580513927113529 1106 4 0.27989982088924137 1106 5 0.4239789613007474 1106 6 0.4093046997052312 1106 7 0.4663276771266496 1106 8 0.26679890791050553 1106 9 0.3689237682659581 1106 10-11 0.369185978691128 1106 12-13 0.41373097588153485 1106 14-15 0.5207337384286959 1106 16-17 0.596784476863931 1106 18-19 0.6558835225977049 1106 20-21 0.43128016608172715 1106 22-23 0.43298829752452406 1106 24-25 0.7494938007771523 1106 26-27 0.5473258660902687 1106 28-29 0.1362231150145945 1106 30-31 0.12079542303914792 1106 32-33 0.3377736331014205 1106 34-35 0.250705546731119 1106 36-37 0.14263565252935706 1106 38-39 0.721174754287425 1106 40-41 0.03365050620641341 1106 42-43 0.7485472782045832 1106 44-45 0.5043442144727592 1106 46-47 0.8652989903150683 1106 48-49 1.3056292980672382 1106 50-51 1.1839907961017992 1106 52-53 1.48226054541977 1106 54-55 1.097039384555483 1106 56-57 1.02121552170113 1106 58-59 1.1924305849584798 1106 60-61 1.2741596747893738 1106 62-63 1.1926965481915452 1106 64-65 1.1570418367548534 1106 66-67 1.4983646184154544 1106 68-69 1.68840920747364 1106 70-71 1.5816782655853814 1106 72-73 1.133158492737845 1106 74-75 1.1217381856715285 1106 76-77 1.1350923509109911 1106 78-79 1.3879287283743729 1106 80-81 1.3933444149143774 1106 82-83 1.0537601658738005 1106 84-85 1.1795300798585764 1106 86-87 1.232118226203255 1106 88-89 1.1580777575838965 1106 90-91 1.387791169404295 1106 92-93 1.1825960116333931 1106 94-95 1.580235061552262 1106 96-97 2.0054774597922425 1106 98-99 1.1068132415925795 1106 100-101 1.3458214951281526 1107 1 -0.22572637654623406 1107 2 -0.04457270486018672 1107 3 0.034082687163696335 1107 4 -0.16569050628003623 1107 5 -0.1743496304488943 1107 6 0.01261195432827833 1107 7 0.08954958324329709 1107 8 -0.15176439223174043 1107 9 -0.24327395577387279 1107 10-11 0.01952381652896662 1107 12-13 -0.2717242161526059 1107 14-15 -0.17245616199804914 1107 16-17 0.24632217245425636 1107 18-19 0.12227754820224845 1107 20-21 0.1083783168640835 1107 22-23 0.08625999026990172 1107 24-25 0.08013035838740024 1107 26-27 -0.2527274767262355 1107 28-29 -0.014381436905743783 1107 30-31 -0.12120315448574814 1107 32-33 -0.04593902692703011 1107 34-35 0.10436842013083236 1107 36-37 -0.1590179747821665 1107 38-39 -0.6172854164095867 1107 40-41 -0.283828156666992 1107 42-43 -0.7770572737157622 1107 44-45 -0.4280882179596759 1107 46-47 -1.0768240537816638 1107 48-49 -1.3366182126013229 1107 50-51 -1.7115461882509742 1107 52-53 -1.8807373208675706 1107 54-55 -1.9840416965255976 1107 56-57 -2.1066294285122353 1107 58-59 -1.9519506383701106 1107 60-61 -2.1340906808294022 1107 62-63 -1.446794916957817 1107 64-65 -1.1413223168724578 1107 66-67 -1.7572363773171205 1107 68-69 -2.0226505364808425 1107 70-71 -2.7768743659935637 1107 72-73 -2.750909786067279 1107 74-75 -2.2282795952673062 1107 76-77 -2.3925570089752135 1107 78-79 -2.5120534906867924 1107 80-81 -2.6356385153843433 1107 82-83 -2.6908166477819613 1107 84-85 -2.4896555531428497 1107 86-87 -2.458493438092617 1107 88-89 -2.4264706065697297 1107 90-91 -2.6737308789598586 1107 92-93 -2.273929592918524 1107 94-95 -2.074192392217295 1107 96-97 -2.3152906767653683 1107 98-99 -2.109847498094471 1107 100-101 -2.6528449344593277 1108 1 0.21031828055105706 1108 2 -0.28623937152685386 1108 3 -0.11942608476612548 1108 4 -0.19950230851289064 1108 5 -0.014022676700889747 1108 6 -0.13471659431606042 1108 7 0.16542677622575042 1108 8 0.006848047959650216 1108 9 0.06904661838880344 1108 10-11 0.2100541195592669 1108 12-13 0.08991852706908077 1108 14-15 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-0.04811456466970654 2316 96-97 0.23064997273070986 2316 98-99 -0.3624650792814812 2316 100-101 0.12468866016553193 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 14.0 14 87.0 15 377.0 16 1155.0 17 2505.0 18 3468.0 19 4210.0 20 5403.0 21 6969.0 22 6517.0 23 4608.0 24 4527.0 25 4726.0 26 4969.0 27 5599.0 28 6171.0 29 5138.0 30 3021.0 31 2288.0 32 2520.0 33 3118.0 34 3931.0 35 6430.0 36 10816.0 37 26084.0 38 27698.0 39 6645.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.63672162497091 18.666532468594507 13.541004850002203 18.155741056432387 2 11.970638413064144 26.73767839493349 41.06530183132197 20.226381360680403 3 23.976553016723585 33.561639527789836 32.32513821015491 10.136669245331673 4 8.898910042327843 22.484700969200677 35.76797046485154 32.84841852361994 5 7.402026453329308 43.239809556155144 34.32769171745379 15.030472273061758 6 20.082774063288195 40.19762622101178 17.252353966047536 22.467245749652488 7 18.52902690052593 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18.83933092025196 30-31 18.97991786008541 28.395818789033754 33.50409127216237 19.12017207871847 32-33 14.983616043069995 27.69612005257961 38.49129228853375 18.828971615816652 34-35 15.46349931130776 27.46687044409643 35.950992786027406 21.1186374585684 36-37 16.16791511789531 28.67821405435323 38.70764857198564 16.446222255765832 38-39 16.51100335226451 27.62599294326308 36.18936206343516 19.67364164103725 40-41 14.809084448134241 29.854651345622873 35.66199362252118 19.674270583721707 42-43 17.131769781819784 29.467222651999727 33.86699120109184 19.53401636508865 44-45 16.282068215123555 28.16814153726171 33.990578438586894 21.559211809027843 46-47 20.626175336641573 28.064680465669163 32.118845009654265 19.190299188034995 48-49 18.15348717271395 26.623772775586964 33.39151053164525 21.83122952005384 50-51 11.553991584746882 27.93920640012076 38.65104373038485 21.855758284747512 52-53 14.069133379875092 30.96756542576275 33.77139191305496 21.191909281307193 54-55 12.43859947043026 28.28323804851664 31.57135040283779 27.706812078215314 56-57 18.849412253061377 25.92784769523953 28.91721227444543 26.30552777725366 58-59 18.85947533601263 27.20931841481286 31.570721460153333 22.360484789021175 60-61 17.619514833613213 31.66632074819022 30.321012346144894 20.393152072051674 62-63 12.72363054300279 34.42613155718244 29.658072083801112 23.19216581601366 64-65 11.988628680505546 37.445792831917686 27.538027566644548 23.02755092093222 66-67 15.50689635653503 31.28423806738492 26.38823374025925 26.8206318358208 68-69 14.97229507474984 30.79995220035598 28.057762096140177 26.169990628754004 70-71 17.85914199638987 29.660936998811298 28.126002377403346 24.353918627395483 72-73 17.33177355547589 25.252048780794606 32.583948124807385 24.832229538922117 74-75 15.234878645509035 30.911589526846416 28.293615602810117 25.559916224834435 76-77 16.087095982943076 26.4278571293798 29.741441662421302 27.743605225255823 78-79 17.39686912331679 25.790738190028744 30.793977244853675 26.01841544180079 80-81 19.12834833361636 24.708013358742615 30.11849280175098 26.045145505890048 82-83 17.439951697201835 24.28096127599892 31.680157487248188 26.598929539551058 84-85 16.364145235444695 26.719057592281615 29.59206777486368 27.324729397410014 86-87 16.6729560935112 26.700818254432473 27.09296401818902 29.53326163386731 88-89 16.0081636760442 26.066844028503684 24.93411825380353 32.99087404164858 90-91 18.37267369824588 22.38752932445266 25.862437656056404 33.37735932124505 92-93 19.28526953338742 24.63788624942609 26.117159443259936 29.959684773926554 94-95 18.583055026195463 23.31993685415448 26.43792021233105 31.659087907319005 96-97 15.798411290779072 22.743825355195384 29.880123524343226 31.57763982968232 98-99 18.898529212907203 24.49645117974295 24.35902677102955 32.2459928363203 100-101 17.037903588466342 22.635860763354938 25.599466651571902 34.72676899660682 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 128.0 1 82.5 2 96.5 3 171.0 4 128.5 5 76.0 6 107.5 7 193.0 8 260.5 9 263.0 10 191.5 11 120.5 12 133.0 13 146.0 14 156.5 15 166.0 16 164.0 17 196.0 18 225.0 19 256.0 20 295.0 21 354.5 22 441.0 23 519.5 24 642.0 25 788.5 26 1003.5 27 1227.5 28 1588.5 29 1929.0 30 2153.5 31 2550.5 32 3275.5 33 4423.5 34 5224.0 35 5169.0 36 5210.0 37 6037.5 38 5964.5 39 5409.5 40 5113.0 41 4409.5 42 4176.0 43 4010.5 44 3716.5 45 3870.0 46 4360.5 47 8443.5 48 16904.0 49 16642.5 50 10802.0 51 8077.5 52 4977.0 53 2630.5 54 1149.0 55 603.5 56 391.5 57 266.0 58 220.0 59 210.5 60 190.5 61 118.0 62 68.0 63 45.0 64 29.5 65 28.0 66 18.5 67 18.0 68 17.5 69 13.0 70 18.0 71 16.0 72 8.0 73 3.5 74 3.5 75 5.0 76 4.5 77 4.0 78 2.0 79 3.5 80 4.5 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017610395164688642 2 0.09434140266797486 3 0.0 4 0.0 5 0.0 6 0.006289426844531658 7 0.025786650062579797 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0015723567111329145 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 9.434140266797486E-4 64-65 3.144713422265829E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.144713422265829E-4 100-101 9.434140266797486E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 158997.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.66021371472418 #Duplication Level Percentage of deduplicated Percentage of total 1 79.57356729322349 19.623011754938773 2 8.668928052232907 4.275552368912621 3 3.4124818281516998 2.5245759353950072 4 1.787854829248387 1.7635552872066769 5 1.1043382896783902 1.361660911841104 6 0.8263408911219363 1.2226645785769543 7 0.5610956668111913 0.9685717340578752 8 0.425922619806677 0.8402674264294295 9 0.35196000918156545 0.7811468140908319 >10 2.626947894616032 12.9134511971924 >50 0.311153051595297 5.340981276376284 >100 0.2550434849141779 12.887664547129821 >500 0.04335739243541024 6.691321219897231 >1k 0.04590782728455202 19.91295433247168 >5k 0.005100869698283557 8.892620615483311 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 7672 4.825248275124688 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6467 4.067372340358623 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 2979 1.873620256985981 RNA PCR Primer, Index 27 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2894 1.8201601288074618 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2731 1.7176424712415959 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2270 1.4276998937086864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2205 1.3868186192192304 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1810 1.13838625886023 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1784 1.1220337490644476 No Hit ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 1763 1.1088259526909312 RNA PCR Primer, Index 27 (96% over 25bp) TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1581 0.9943583841204551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.9880689572759234 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1471 0.9251746888306069 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1430 0.8993880387680271 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 1360 0.8553620508563055 RNA PCR Primer, Index 27 (96% over 30bp) GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 1317 0.8283175154248192 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1265 0.7956124958332547 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1120 0.7044158065875457 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1077 0.6773712711560596 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1033 0.6496977930401202 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 999 0.6283137417687127 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 931 0.5855456392258974 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 841 0.5289407976251125 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 695 0.4371151656949502 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 621 0.39057340704541593 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 616 0.38742869362315013 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 592 0.37233406919627415 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 575 0.36164204356057034 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 558 0.3509500179248665 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 549 0.34528953376478805 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 534 0.33585539349799054 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 532 0.3345975081290842 No Hit CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT 531 0.33396856544463105 RNA PCR Primer, Index 27 (96% over 28bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 531 0.33396856544463105 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 525 0.330194909337912 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 508 0.3195028837022082 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 501 0.31510028491103603 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 477 0.3000056604841601 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 474 0.29811883243080056 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 458 0.2880557494795499 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 422 0.26541381283923593 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 386 0.24277187619892202 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC 378 0.23774033472329664 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 361 0.22704830908759285 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 361 0.22704830908759285 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 359 0.2257904237186865 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 358 0.22516148103423334 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 354 0.2226457102964207 No Hit CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA 350 0.22012993955860802 RNA PCR Primer, Index 27 (96% over 30bp) CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG 343 0.21572734076743585 RNA PCR Primer, Index 27 (100% over 24bp) GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 329 0.20692214318509153 No Hit TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 317 0.19937483097165354 No Hit CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT 317 0.19937483097165354 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 306 0.19245646144266873 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 305 0.19182751875821558 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 295 0.18553809191368392 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 292 0.1836512638603244 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 289 0.1817644358069649 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 279 0.17547500896243326 No Hit TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT 274 0.17233029554016743 RNA PCR Primer, Index 27 (95% over 22bp) CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA 274 0.17233029554016743 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 273 0.17170135285571425 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 269 0.1691855821179016 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 268 0.16855663943344842 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 262 0.16478298332672944 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 256 0.16100932722001043 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 254 0.1597514418511041 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 250 0.15723567111329145 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 235 0.14780153084649397 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 235 0.14780153084649397 No Hit GCCATGAAGTCATTCTAAATTTGTTGAAGCATACGATACTGATATTACTT 229 0.14402787473977496 No Hit GATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAGATCAT 225 0.14151210400196232 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 219 0.1377384478952433 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 217 0.13648056252633697 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 214 0.13459373447297748 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 207 0.13019113568180532 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 207 0.13019113568180532 No Hit ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC 205 0.12893325031289898 TruSeq Adapter, Index 27 (96% over 25bp) ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 202 0.1270464222595395 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.12578853689063316 No Hit GATTTAAATGATGAGAAATTATTGGCTGGACACAAATTGCAGAAAAGTCA 197 0.12390170883727367 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 191 0.12012805273055466 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 191 0.12012805273055466 No Hit ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT 190 0.1194991100461015 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.117612281992742 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 179 0.11258074051711667 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 175 0.11006496977930401 No Hit TATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTCATGGCTGCCCT 172 0.1081781417259445 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 162 0.10188871488141285 No Hit CATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTT 161 0.1012597721969597 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 159 0.10000188682805335 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0012578853689063315 0.0 3 0.0 0.0 0.0 0.0012578853689063315 0.0 4 0.0 0.0 0.0 0.0012578853689063315 0.0 5 0.0 0.0 0.0 0.0012578853689063315 0.0 6 0.0 0.0 0.0 0.0012578853689063315 0.0 7 0.0 0.0 0.0 0.0012578853689063315 0.0 8 0.0 0.0 0.0 0.0012578853689063315 0.0 9 0.0 0.0 0.0 0.002515770737812663 0.0 10-11 0.0 0.0 0.0 0.0037736561067189946 0.0 12-13 0.0 0.0 0.0 0.0056604841600784925 0.0 14-15 0.0 0.0 0.0 0.00974861160902407 0.0 16-17 0.0 0.0 0.0 0.014780153084649396 0.0 18-19 0.0 0.0 0.0 0.024843236035900048 0.0 20-21 0.0 0.0 0.0 0.033962904960470955 0.0 22-23 0.0 0.0 0.0 0.06383768247199632 0.0 24-25 0.0 0.0 0.0 0.12044252407278125 0.0 26-27 0.0 0.0 0.0 0.21541286942520926 0.0 28-29 0.0 0.0 0.0 0.3657301710095159 0.0 30-31 0.0 0.0 0.0 0.6059862764706252 0.0 32-33 0.0 0.0 0.0 0.9625967785555702 0.0 34-35 0.0 0.0 0.0 1.4827323785983384 0.0 36-37 0.0 0.0 0.0 2.0645043617175167 0.0 38-39 0.0 0.0 0.0 2.7704925250161954 0.0 40-41 0.0 0.0 0.0 3.7959835720170823 0.0 42-43 0.0 0.0 0.0 4.442851122977164 0.0 44-45 0.0 0.0 0.0 4.940344786379617 0.0 46-47 0.0 0.0 0.0 5.487524921853872 0.0 48-49 0.0 0.0 0.0 5.993509311496443 0.0 50-51 0.0 0.0 0.0 6.5073554846946795 0.0 52-53 0.0 0.0 0.0 7.0601961043290125 0.0 54-55 0.0 0.0 0.0 7.542280671962365 0.0 56-57 0.0 0.0 0.0 8.014302156644465 0.0 58-59 0.0 0.0 0.0 8.386007283156285 0.0 60-61 0.0 0.0 0.0 8.708654880280761 0.0 62-63 0.0 0.0 0.0 9.041365560356486 0.0 64-65 0.0 0.0 0.0 9.384453794725687 0.0 66-67 0.0 0.0 0.0 9.75301420781524 0.0 68-69 0.0 0.0 0.0 10.225664635181797 0.0 70-71 0.0 0.0 0.0 10.728818782744328 0.0 72-73 0.0 0.0 0.0 11.154612980119122 0.0 74-75 0.0 0.0 0.0 11.57443222199161 0.0 76-77 0.0 0.0 0.0 12.000226419366403 0.0 78-79 0.0 0.0 0.0 12.524450146858118 0.0 80-81 0.0 0.0 0.0 13.082322307968075 0.0 82-83 0.0 0.0 0.0 13.555916149361309 0.0 84-85 0.0 0.0 0.0 14.046805914577005 0.0 86-87 0.0 0.0 0.0 14.536437794423794 0.0 88-89 0.0 0.0 0.0 15.113492707409574 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCTAG 15 6.608305E-4 94.99654 7 GCTAGTA 15 6.608305E-4 94.99654 9 GACATAT 110 0.0 77.77338 1 GGGAACA 20 0.002071079 71.247406 7 ACATATA 130 0.0 65.808235 2 ACAGTAC 25 0.0050140624 56.997925 8 CAGTACA 25 0.0050140624 56.997925 9 GGCTCTG 25 0.0050140624 56.997925 8 TACACAG 25 0.0050140624 56.997925 5 TGTATAT 35 2.7724868E-4 54.317905 2 ATATATT 35 2.7811227E-4 54.283733 5 ATATTTA 35 2.7811227E-4 54.283733 7 AGTACTC 155 0.0 52.094875 5 AAGTACT 160 0.0 50.46691 4 CATATAG 170 0.0 50.292286 3 ATTTATG 40 5.375058E-4 47.498272 9 AAAGTAC 180 0.0 47.49827 3 ATATAGG 190 0.0 44.998363 4 TAGGATA 190 0.0 44.998363 7 AGGATAT 195 0.0 43.84456 8 >>END_MODULE