FastQCFastQC Report
Fri 10 Feb 2017
SRR2568686_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568686_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425738
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38600.039.6268121
TCAACGC41350.036.0693974
GGTATCA23650.034.9493181
CAACGCA42800.034.847425
ATCAACG43100.034.4939423
AACGCAG44500.033.516866
GTACATG88450.030.7735311
TATCAAC48950.030.762892
TACATGG90150.028.9812772
ACATGGG93650.026.9316233
ACGCAGA54900.026.9080227
CGCAGAG56700.025.6349288
CATGGGG59300.022.9885714
GAGTACA59700.021.1654681
GCAGAGT69650.020.5276419
GTATAGC8200.020.2756481
AGTACAT61750.019.5396752
GTGTAGG11150.018.3195231
GTACCTG17900.018.3111931
TACCTGG16900.018.2703652
Produced by FastQC (version 0.11.5)