FastQCFastQC Report
Fri 10 Feb 2017
SRR2568686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425738
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.039.8343241
GTATCAA38600.036.10381
TCAACGC42400.031.6008634
CAACGCA43800.030.4823075
GTACATG82600.030.1157881
ATCAACG44750.030.0475483
AACGCAG46700.028.5894036
TACATGG89000.027.6783772
TATCAAC50450.027.5246982
ACATGGG86850.027.4083943
ACGCAGA56800.023.3374617
CGCAGAG58600.022.6951378
CATGGGG56350.021.6697984
GAGTACA58650.020.5986291
TAATACT18450.019.5718964
TATACCG1951.030917E-519.4926575
AGTACAT58200.019.2019672
CATGGGA35100.018.4097334
TACACCG2603.2741445E-718.2743665
GCAGAGT72100.018.1163149
Produced by FastQC (version 0.11.5)