FastQCFastQC Report
Fri 10 Feb 2017
SRR2568683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983347
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39550.040.9562531
TCAACGC43300.035.5418744
CAACGCA44300.034.846795
GGTATCA22850.034.3012581
ATCAACG46550.032.9589463
AACGCAG48600.031.960186
TATCAAC51600.030.0109832
GTACATG89700.028.9142881
ACATGGG89700.026.8476313
ACGCAGA58850.026.2321957
TACATGG97450.025.8348732
CGCAGAG60100.025.3704598
CATGGGG60000.023.6702654
GCAGAGT74050.020.2061359
TATACCG2601.4928446E-820.0956735
GTATATA17350.019.7126661
TAATACT24800.018.769714
GAGTACA67050.018.4907281
AGAGTAC65400.017.6854210-11
GTATAAA17300.017.2984331
Produced by FastQC (version 0.11.5)