FastQCFastQC Report
Fri 10 Feb 2017
SRR2568683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2983347
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40650.038.383841
GGTATCA23600.035.6776581
TCAACGC43750.033.883984
ATCAACG45550.032.7536053
CAACGCA45450.032.721135
AACGCAG48900.030.4125826
TATCAAC53200.028.4262392
GTACATG82850.028.421551
ACATGGG82200.026.30013
TACATGG87700.025.9740582
ACGCAGA57150.025.77297
CGCAGAG58150.025.076158
CATGGGG52700.022.9904484
GCAGAGT72950.019.5980769
GAGTACA60250.019.5807691
GGACCGT4650.019.4141546
GTGTAGC17850.018.6549851
GACCGTA2302.0544721E-618.5922747
GTATAGT10700.018.2278391
AGTACAT59700.017.763742
Produced by FastQC (version 0.11.5)