FastQCFastQC Report
Fri 10 Feb 2017
SRR2568682_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568682_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029803
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.039.5737651
GTATCAA64800.038.6317251
TCAACGC73100.033.4643334
CAACGCA74700.032.6203845
ATCAACG77200.031.5017133
GTACATG162700.030.5972351
AACGCAG80600.030.5865156
TATCAAC84100.029.0883922
TACATGG170900.028.378682
ACATGGG166100.028.310473
CATGGGG100000.026.1724224
ACGCAGA94650.025.9458337
CGCAGAG96000.025.333578
TATACCG3007.2759576E-1222.16665
GTATATA22250.021.5625341
TAATACT35550.021.1110484
GAGTACA115650.020.4135821
GCAGAGT118500.020.0423839
ATACCGT6050.019.6282846
AGAGTAC100400.019.18433610-11
Produced by FastQC (version 0.11.5)