FastQCFastQC Report
Fri 10 Feb 2017
SRR2568682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4029803
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35450.040.3937871
GTATCAA61350.039.7802161
TCAACGC70600.033.6512374
CAACGCA72450.032.7919585
ATCAACG74000.032.040893
GTACATG153200.031.7673631
AACGCAG78200.030.5023086
ACATGGG157350.029.7745953
TATCAAC82150.029.1825052
TACATGG164650.029.062692
ACGCAGA91250.025.9828767
CATGGGG94100.025.5503374
CGCAGAG94050.025.0490658
TATACCG3150.022.6264515
CATGGGA58750.021.3516674
TAATACT36650.020.22494
GCAGAGT118400.019.937629
AGAGTAC101150.019.22899810-11
GAGTACA109600.018.8817841
TTAATAC38500.018.14233
Produced by FastQC (version 0.11.5)