FastQCFastQC Report
Fri 10 Feb 2017
SRR2568681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148934
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24931.6738958196248002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.9923858890515261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.8795842453704326No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA9920.6660668484026482No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5530.37130541045026655No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4610.3095330817677629No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.3021472598600723No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.2954328763076262No Hit
GTACATGGGTGTGTGTGTGCAGCTTGATCGGGGCCATGGCCCAAACCCCT4090.27461828729504345No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3950.26521815032161894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.25917520512441755No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3760.25246082157197147No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA3350.22493184900694266No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.20613157506009375No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2970.19941719150764767No Hit
GTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGAG2860.19203136959995704No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2720.18263123262653255No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA2500.16785958881115123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.15845945183772678No Hit
GTCGTAGAGGACACAGGCGCCTTGTTCCCCGCAGAAGGTGCTCCAGAACA2240.1504021915747915No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2210.1483878765090577No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2120.14234493131185627No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2090.14033061624612245No Hit
CTATAAAACAGATTCCATGTTTTCACACCACTCATGGTCTTCCAGGTTAT2060.13831630118038862No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.13764486282514402No Hit
GTACATGGGGTGTTCCTTTGGTTGTTGGTTTTATCTCCCCCTCACTTTTT1990.1336162326936764No Hit
GGTATATTGGGGGGAAAAAAAGCAGTCTAATAAAATCCTAGACAGAGTTT1930.12958760256220878No Hit
CATCAGGACAGTCAGCCCTGAACTCAAGTCTTATGCGCTGGGAGTTCTTT1930.12958760256220878No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1920.12891616420696417No Hit
CTTTAACAGTGTGTTCCACGTATTCTTTTCCTGCCTGAATTACATCCAGG1870.12555897243074113No Hit
TCTTAATACCAGTCCAGAGTTCAACACGGCTGTTGTCCGAGCGCAGCAGC1800.1208589039440289No Hit
GTACATGGGATGGAGACGGTGGACAAGCGCGAGAAACTGGCGGAGGGCGC1790.12018746558878429No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA1760.11817315052305048No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.11750171216780586No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA1740.11683027381256127No Hit
GTGTAGAAATGCCAACAGCTGGGAAAGGGAGGGACCGCTATTTCAGTCAG1680.11280164368109363No Hit
CTCTACGACAATGTGGTCTACAGATACCTGTACGTCAGCATCGCCATTGC1660.11145876697060443No Hit
GGTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGA1640.11011589026011522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1620.10877301354962601No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1610.10810157519438142No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1570.105415821773403No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.10407294506291377No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1550.10407294506291377No Hit
ACAGATACCTGTACGTCAGCATCGCCATTGCGCTCAAGTCCTTTGCCTTC1530.10273006835242456No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1530.10273006835242456No Hit
GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1520.10205862999717996No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT1500.10071575328669076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGT156.60717E-494.997657
ACTAGTC200.002070725271.248248
CTTCTAT250.005013210756.998591
AGTAATG250.005013210756.998592
TTCTATC352.780389E-454.284372
TGGACCG352.780389E-454.284375
ATGTGGA352.780389E-454.284372
GTGGACC352.780389E-454.284374
AGGGGCC352.780389E-454.284376
GGACCGT352.780389E-454.284376
GTGGGTA558.143652E-751.81699
GAGTAAT405.373647E-447.4988251
TCTTAGG405.373647E-447.4988252
GGGAGTG707.860399E-847.4988255
TAACCGG205.382369E-447.49882514-15
CGGGAGT601.4837315E-647.4988254
GGCCTTA405.373647E-447.4988259
AGTGGGT652.5745812E-643.8450668
GTCGGGA652.5745812E-643.8450662
ACTGATT554.9431572E-543.1807488
Produced by FastQC (version 0.11.5)