##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568681_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 148934 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.26354626881706 33.0 31.0 34.0 23.0 34.0 2 30.302160688627175 33.0 31.0 34.0 24.0 34.0 3 30.305705883142867 33.0 31.0 34.0 23.0 34.0 4 33.5053647924584 37.0 35.0 37.0 25.0 37.0 5 33.56256462594169 37.0 35.0 37.0 25.0 37.0 6 33.81342070984463 37.0 35.0 37.0 26.0 37.0 7 33.79939436260357 37.0 35.0 37.0 26.0 37.0 8 33.81998737695892 37.0 35.0 37.0 26.0 37.0 9 35.419722830246954 39.0 37.0 39.0 27.0 39.0 10-11 35.34652262075819 39.0 37.0 39.0 25.5 39.0 12-13 35.311211677655876 39.0 37.0 39.0 25.0 39.0 14-15 36.35940416560356 40.0 37.0 41.0 25.0 41.0 16-17 36.37770421797575 40.0 37.0 41.0 25.0 41.0 18-19 36.50517343252716 40.0 37.0 41.0 25.0 41.0 20-21 36.5332361985846 40.0 37.5 41.0 25.0 41.0 22-23 36.401053486779375 40.0 36.5 41.0 25.0 41.0 24-25 36.25910470409711 40.0 36.0 41.0 24.0 41.0 26-27 36.058898572522054 40.0 35.5 41.0 24.0 41.0 28-29 35.79828313212564 40.0 35.0 41.0 22.5 41.0 30-31 35.40869781245384 39.0 35.0 41.0 21.5 41.0 32-33 34.92900882269999 39.0 35.0 41.0 19.0 41.0 34-35 34.56689204614125 39.0 35.0 41.0 16.0 41.0 36-37 34.2083473216324 38.0 34.5 41.0 15.0 41.0 38-39 33.853015429653404 38.0 34.0 41.0 13.0 41.0 40-41 33.39327823062565 38.0 33.0 41.0 11.0 41.0 42-43 32.871013334765735 37.5 33.0 40.0 9.5 41.0 44-45 32.198725610001745 37.0 30.5 40.0 8.0 41.0 46-47 31.570595028670418 36.5 28.5 40.0 8.0 41.0 48-49 31.03681832220984 36.5 25.0 40.0 7.0 41.0 50-51 29.779684289685363 35.5 23.0 39.0 7.0 40.0 52-53 29.686539003854058 35.0 21.5 39.5 7.0 40.5 54-55 29.83261041803752 35.0 20.0 40.0 7.0 41.0 56-57 29.47860461681013 35.0 18.5 40.0 7.0 41.0 58-59 29.01088065854674 35.0 16.0 40.0 7.0 41.0 60-61 28.59822471698873 35.0 14.0 39.0 7.0 41.0 62-63 28.145453690896638 35.0 12.5 39.0 7.0 41.0 64-65 27.80413807458337 35.0 11.0 39.0 7.0 41.0 66-67 27.452203660681914 35.0 10.0 37.5 7.0 41.0 68-69 27.004451636295272 35.0 10.0 37.0 7.0 40.0 70-71 26.559791585534533 34.0 10.0 36.5 7.0 39.5 72-73 26.144329031651605 34.0 10.0 36.0 7.0 39.0 74-75 25.773956920515126 34.0 10.0 35.0 7.0 39.0 76-77 25.437415230907654 33.5 10.0 35.0 7.0 37.0 78-79 25.180301341533834 33.0 10.0 35.0 7.0 37.0 80-81 24.888148441591575 33.0 10.0 35.0 7.0 36.5 82-83 24.67253615695543 33.0 9.0 35.0 7.0 36.0 84-85 24.422425369626815 32.5 8.0 35.0 7.0 36.0 86-87 24.145423476170656 32.0 8.0 35.0 7.0 35.0 88-89 23.93162071790189 32.0 8.0 35.0 7.0 35.0 90-91 23.746767024319496 31.5 8.0 35.0 7.0 35.0 92-93 23.541004740354786 31.0 8.0 35.0 7.0 35.0 94-95 23.35867229779634 31.0 8.0 35.0 7.0 35.0 96-97 23.177202653524382 30.0 8.0 35.0 7.0 35.0 98-99 23.004790712664672 30.0 7.5 35.0 7.0 35.0 100-101 21.841493547477405 26.5 7.0 34.5 5.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4675075844030978 1101 2 0.2605264527293514 1101 3 1.0089039951563201 1101 4 0.2897793923167882 1101 5 0.7042725490393025 1101 6 0.11282485017576249 1101 7 0.7222697157991007 1101 8 0.9783654780428108 1101 9 0.843975835810781 1101 10-11 0.914047679264165 1101 12-13 0.6845601707882096 1101 14-15 0.8862647426701145 1101 16-17 0.6326151441735419 1101 18-19 0.7915640268924946 1101 20-21 0.5451097022159885 1101 22-23 0.8389898348310538 1101 24-25 0.8617684405695272 1101 26-27 0.6871099056345216 1101 28-29 1.2497587996412847 1101 30-31 0.9515384007579542 1101 32-33 1.382614707085331 1101 34-35 1.5457160683824824 1101 36-37 1.4069925425163916 1101 38-39 0.7719085814434834 1101 40-41 0.6790875541541084 1101 42-43 0.6674821062972285 1101 44-45 1.3929653144390457 1101 46-47 1.433044898656945 1101 48-49 0.9865092234493105 1101 50-51 1.0264800906985982 1101 52-53 1.1475609444974992 1101 54-55 1.066871969088691 1101 56-57 0.6389261567391316 1101 58-59 0.9673472148974334 1101 60-61 0.17644927091599172 1101 62-63 0.3762932102395915 1101 64-65 0.327913957921357 1101 66-67 0.2153502742103086 1101 68-69 0.15062736697404233 1101 70-71 0.1335158340341316 1101 72-73 0.46135594010483416 1101 74-75 0.1552711578595094 1101 76-77 0.11735167564684446 1101 78-79 -0.01143421046997517 1101 80-81 0.1658825167094342 1101 82-83 0.33636926193970496 1101 84-85 0.32697789431530566 1101 86-87 -0.2097287325482462 1101 88-89 0.29341814379480624 1101 90-91 0.36603118564787707 1101 92-93 0.6543799576991809 1101 94-95 0.34555001281167463 1101 96-97 0.03513305879784667 1101 98-99 0.23686763129455457 1101 100-101 0.5836952927724859 1102 1 -0.44670362811320175 1102 2 -0.18875646774000998 1102 3 -0.21221725647340506 1102 4 -0.5259963573572648 1102 5 -0.10146408720581235 1102 6 -0.26162104291419297 1102 7 -0.006934977812370846 1102 8 -0.029717963938935554 1102 9 0.039803736723428074 1102 10-11 -0.07245818775017909 1102 12-13 0.16572053584688717 1102 14-15 -0.13446537467016384 1102 16-17 0.1406985861552883 1102 18-19 -0.025776259939313206 1102 20-21 -0.18768038904867268 1102 22-23 0.2706065232274 1102 24-25 -0.09833586190766397 1102 26-27 0.3107083410973672 1102 28-29 0.20210560537791622 1102 30-31 0.7499738636002036 1102 32-33 0.16455734072288664 1102 34-35 0.2810485325285015 1102 36-37 0.17152970027389358 1102 38-39 0.36649789043957526 1102 40-41 0.4710693012206022 1102 42-43 0.9779775430638509 1102 44-45 0.9457032544651156 1102 46-47 1.0463434644978875 1102 48-49 0.6874870591729092 1102 50-51 0.5612258534104626 1102 52-53 1.117313226114188 1102 54-55 1.09920573701568 1102 56-57 1.1359274605200973 1102 58-59 1.1549612957318516 1102 60-61 0.6928117220242065 1102 62-63 0.9064757395746632 1102 64-65 0.9110299944272207 1102 66-67 1.0122211998947925 1102 68-69 0.608710808955788 1102 70-71 0.9507257427694675 1102 72-73 0.8768057445376876 1102 74-75 0.7210469075335624 1102 76-77 0.636778012022333 1102 78-79 0.8955410176916949 1102 80-81 0.34841185178114387 1102 82-83 0.3136182841039812 1102 84-85 0.6034446479007052 1102 86-87 0.16452159339699435 1102 88-89 0.126077856962997 1102 90-91 0.12463614001554646 1102 92-93 0.8401687451828828 1102 94-95 0.22201676639707557 1102 96-97 -0.2030677234707312 1102 98-99 0.2775455973962515 1102 100-101 -0.017152164854636 1103 1 0.06104651647904902 1103 2 0.2980567190731769 1103 3 0.45678216515759473 1103 4 0.6633616530533786 1103 5 0.4077898167849341 1103 6 0.38840787553577627 1103 7 0.3294444322512504 1103 8 0.2166093929147408 1103 9 0.038126466625108435 1103 10-11 0.35064765377671137 1103 12-13 0.5684967070441118 1103 14-15 0.39101177975435775 1103 16-17 0.03534867291063648 1103 18-19 0.4677460072671593 1103 20-21 0.3086180493550259 1103 22-23 0.36164180373636157 1103 24-25 0.2751748379188328 1103 26-27 0.37571701663235757 1103 28-29 0.03834042319168418 1103 30-31 0.2860930076140846 1103 32-33 0.13028897750714918 1103 34-35 0.030614755778934466 1103 36-37 -0.3919752158626011 1103 38-39 -0.5608300447830885 1103 40-41 -0.7714986571368243 1103 42-43 -0.760483995397685 1103 44-45 -0.6450428415441358 1103 46-47 -0.8965715152591969 1103 48-49 -0.7726343983169706 1103 50-51 -0.7703530939579579 1103 52-53 -0.9269898392415108 1103 54-55 -0.3691863971659295 1103 56-57 0.013081885042939945 1103 58-59 0.019883215916934205 1103 60-61 -0.02442367415855884 1103 62-63 0.2430575787880791 1103 64-65 -0.10906832830267987 1103 66-67 0.40325648040375484 1103 68-69 0.05712029420737963 1103 70-71 0.17521966989497528 1103 72-73 -0.4962711785392351 1103 74-75 -0.15440132844098997 1103 76-77 -0.1352289284056596 1103 78-79 -0.8309193640318462 1103 80-81 -0.49456096487588397 1103 82-83 -0.3427437748896587 1103 84-85 -0.31737663607847466 1103 86-87 -0.281603334306876 1103 88-89 -0.3781442367327834 1103 90-91 0.3239131787662686 1103 92-93 -0.532156298194792 1103 94-95 -0.21609775066481163 1103 96-97 -0.36868368645511396 1103 98-99 -0.4916273349345772 1103 100-101 -0.3976032926741837 1104 1 -0.20728055119012367 1104 2 -0.35149685235539607 1104 3 -0.2120970641657145 1104 4 -0.15195789581880348 1104 5 -0.32922851028273215 1104 6 -0.46450565829881185 1104 7 -0.0029686316585255668 1104 8 -0.36180931009278083 1104 9 -0.17257607096887995 1104 10-11 -0.10773463005786965 1104 12-13 -0.2682337910761916 1104 14-15 0.3374697214836786 1104 16-17 -0.035022567690866424 1104 18-19 -0.15930991378547077 1104 20-21 0.0948316301820924 1104 22-23 -0.3211002075418321 1104 24-25 -0.19821566959996773 1104 26-27 -0.19656329351801816 1104 28-29 0.1423700284548417 1104 30-31 0.01662049821558753 1104 32-33 0.25079532149211303 1104 34-35 -0.22646348670226502 1104 36-37 -0.19854241511072246 1104 38-39 -0.5145718210988832 1104 40-41 -0.5405893526255525 1104 42-43 -0.908921495397685 1104 44-45 -0.3429986686118056 1104 46-47 -0.03226230473288183 1104 48-49 0.07522744378829316 1104 50-51 -0.4747116465895367 1104 52-53 -0.30642475465504404 1104 54-55 -0.8146163783689353 1104 56-57 -0.5446013856337508 1104 58-59 -0.30225024272968426 1104 60-61 -0.8207700087450256 1104 62-63 -0.9816252219637995 1104 64-65 -1.064631063265086 1104 66-67 -0.6857355308744388 1104 68-69 -0.450604094890366 1104 70-71 -0.24254348799976455 1104 72-73 -0.5743961785392351 1104 74-75 -0.5670540540048989 1104 76-77 -0.6149046802853597 1104 78-79 -0.3994109053852277 1104 80-81 -1.1666121866803962 1104 82-83 -1.0298913312806341 1104 84-85 -1.1336947751762203 1104 86-87 -1.2048293681414641 1104 88-89 -1.3566745468831591 1104 90-91 -1.544654725369071 1104 92-93 -2.0165019278940406 1104 94-95 -1.6307476566798478 1104 96-97 -1.9945941657784232 1104 98-99 -1.97479815260375 1104 100-101 -1.2523084148546353 1105 1 -0.6649418415127037 1105 2 -1.007242820097332 1105 3 -0.09894182223023051 1105 4 0.011348555794100434 1105 5 -0.3955087522182126 1105 6 -0.13990888410526026 1105 7 -0.7930391961746537 1105 8 -0.4049040681573004 1105 9 -0.4727272806462963 1105 10-11 -0.29460458973529313 1105 12-13 -0.07733157333425567 1105 14-15 -0.24153733496793706 1105 16-17 0.3707234000510695 1105 18-19 0.008280812020977635 1105 20-21 -0.381352845624356 1105 22-23 0.17612761503880847 1105 24-25 -0.024576556696743523 1105 26-27 -0.00213284996963381 1105 28-29 0.18647285103548228 1105 30-31 -0.10976559049409218 1105 32-33 0.12919753923404897 1105 34-35 -0.33898868831516893 1105 36-37 0.025499923598957253 1105 38-39 0.033185235352732434 1105 40-41 -0.08922846552878383 1105 42-43 0.231451488473283 1105 44-45 0.434723105581746 1105 46-47 0.07308043720260216 1105 48-49 -0.6399943304052584 1105 50-51 -0.7194193078798605 1105 52-53 -1.1908753594937558 1105 54-55 -1.8879530719173232 1105 56-57 -2.015871546924071 1105 58-59 -2.52704862982646 1105 60-61 -1.78473170229341 1105 62-63 -1.6139336897057355 1105 64-65 -1.7394042487489578 1105 66-67 -1.983870611519599 1105 68-69 -1.974167602954882 1105 70-71 -2.1034305847739567 1105 72-73 -2.149496984990847 1105 74-75 -2.187265747553287 1105 76-77 -1.5458522609305199 1105 78-79 -2.1146326795787758 1105 80-81 -1.9118238802287841 1105 82-83 -1.9719276216032142 1105 84-85 -1.5825355009826687 1105 86-87 -1.934920093947916 1105 88-89 -2.0745424904315435 1105 90-91 -1.298812991498103 1105 92-93 -1.9095210811198484 1105 94-95 -1.8300924147443638 1105 96-97 -1.5332786415848751 1105 98-99 -2.128906015506974 1105 100-101 -2.168765068080443 1106 1 -0.2625863065858063 1106 2 -0.30934307537697947 1106 3 -0.838614600136939 1106 4 -1.0903571763943418 1106 5 -1.1648287800669053 1106 6 -0.6331207662124783 1106 7 -0.6097019949678781 1106 8 -1.1068070618913453 1106 9 -1.0441475637746365 1106 10-11 -1.0341903404895234 1106 12-13 -0.5018500230905758 1106 14-15 -1.0613331112501356 1106 16-17 -0.9962410928707257 1106 18-19 -0.6990468742171245 1106 20-21 -0.7450683248538823 1106 22-23 -0.4177166643763712 1106 24-25 -0.691639680391333 1106 26-27 -0.7940509284101083 1106 28-29 -1.0125872557178184 1106 30-31 -1.2362740611369318 1106 32-33 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1.1021065986100638 1106 98-99 1.267473654950205 1106 100-101 0.4842866840662303 1107 1 0.4717160474493305 1107 2 0.28445127689630567 1107 3 0.2902945174669398 1107 4 0.16819516540568458 1107 5 0.5366834795131865 1107 6 0.7127902600685374 1107 7 0.7154093445319489 1107 8 0.7819513191589209 1107 9 1.264764490255608 1107 10-11 0.6512205144999541 1107 12-13 0.6751920167469407 1107 14-15 0.8759741857693939 1107 16-17 1.0076007316288624 1107 18-19 1.0067721440376616 1107 20-21 0.8352918767807367 1107 22-23 1.1228304897370833 1107 24-25 0.9674412181551375 1107 26-27 0.7233718680466055 1107 28-29 0.4656587189310315 1107 30-31 0.31043958835164176 1107 32-33 0.6861428980227231 1107 34-35 0.29072932792358586 1107 36-37 0.3425396427124099 1107 38-39 0.32298663978546927 1107 40-41 -0.1807360362990238 1107 42-43 0.16808743317374564 1107 44-45 0.6879324538371705 1107 46-47 0.38323514424671146 1107 48-49 -0.2554485766198695 1107 50-51 0.014287077900259959 1107 52-53 -0.288966208736678 1107 54-55 0.24878710802562054 1107 56-57 0.18977361436624918 1107 58-59 0.46581948516147165 1107 60-61 0.5382329674454489 1107 62-63 0.9439168698729361 1107 64-65 0.764902312585253 1107 66-67 0.011383941914679951 1107 68-69 0.45758574354501036 1107 70-71 0.5053306616600999 1107 72-73 0.41189208676688693 1107 74-75 0.39930436436244676 1107 76-77 0.6662390271976335 1107 78-79 0.21294028509096208 1107 80-81 -0.02678438055794885 1107 82-83 -0.15584796393369515 1107 84-85 0.15791874523194238 1107 86-87 0.33869741757282057 1107 88-89 -0.098516596202888 1107 90-91 -0.1461906522398202 1107 92-93 -0.4009353887783931 1107 94-95 0.2989398433201522 1107 96-97 0.447821863133143 1107 98-99 -0.0809578039642922 1107 100-101 0.24883422970318847 1108 1 0.15427008172126833 1108 2 0.05574207169523859 1108 3 -0.27054247555811983 1108 4 -0.07205283252766748 1108 5 -0.44226252927007437 1108 6 -0.5111828734886856 1108 7 0.005041653151600656 1108 8 0.015267430413551608 1108 9 -0.43117971020938484 1108 10-11 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-1.5981850809201177 2316 72-73 -1.971934895353396 2316 74-75 -1.836066775243836 2316 76-77 -2.246680122763234 2316 78-79 -1.8580668788365564 2316 80-81 -1.6635010141140256 2316 82-83 -1.9738902250859418 2316 84-85 -2.133349089335514 2316 86-87 -1.9248929743361565 2316 88-89 -1.9799806862636906 2316 90-91 -1.8526953780239381 2316 92-93 -1.596182514177226 2316 94-95 -1.5107946699541834 2316 96-97 -1.5918355927695735 2316 98-99 -1.629976526497554 2316 100-101 -1.666267209102422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 17.0 7 712.0 8 1625.0 9 1493.0 10 1490.0 11 1737.0 12 1722.0 13 1640.0 14 1400.0 15 1333.0 16 1923.0 17 2995.0 18 3019.0 19 3528.0 20 4903.0 21 5660.0 22 4286.0 23 3721.0 24 3589.0 25 2896.0 26 2543.0 27 2444.0 28 2287.0 29 2035.0 30 2179.0 31 2449.0 32 2943.0 33 3814.0 34 5599.0 35 9497.0 36 15568.0 37 23406.0 38 19911.0 39 4569.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.35792993694728 17.376093685998807 12.200936054202508 18.065040322851406 2 15.578729662035576 23.354664553689542 39.78955285016888 21.277052934106003 3 21.063021204023258 33.41345831039252 27.747861468838547 17.775659016745674 4 12.685484845636323 17.85220298924356 34.30714276122309 35.15516940389703 5 11.321869565509322 42.17601203225612 30.31833106161831 16.183787340616252 6 26.800690261802607 35.64584466423597 18.54373560555701 19.00972946840441 7 25.081247314138377 38.085517834121184 20.12381822088526 16.709416630855177 8 29.360299202975916 30.045189318400045 19.938359889611828 20.656151589012214 9 32.524659732620684 15.246395885232362 18.24048023528306 33.9884641468639 10-11 25.237363021057156 29.25104748603352 23.110160614525142 22.401428878384184 12-13 30.08574440512721 27.076297077169965 25.05891990250519 17.779038615197642 14-15 26.58493023755489 26.088065854673882 27.792176400284692 19.534827507486536 16-17 20.581264184135254 30.130460472424026 28.072166194421687 21.216109149019026 18-19 20.224798316026952 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30.31745605435965 20.073992506747956 44-45 18.6371144265245 30.44335074596801 30.31947036942538 20.600064458082105 46-47 18.864839639966295 30.162391182464305 30.0559657021228 20.916803475446603 48-49 18.674419697712363 29.81817083078741 30.330152889593165 21.17725658190706 50-51 18.91332957322805 29.61888647167835 29.89988719684151 21.56789675825209 52-53 18.605556823828003 29.45432204869271 29.08570239166342 22.854418735815866 54-55 18.28729504344206 29.11020989162985 28.29978379684961 24.30271126807848 56-57 18.75193038527133 28.224246981884594 28.32596989270415 24.69785274013993 58-59 19.509984288342487 26.881705990573007 28.888970953576752 24.719338767507757 60-61 18.696201001786026 26.386184484402484 29.20253266547598 25.7150818483355 62-63 19.02426250642065 25.68293902983546 29.404398607432174 25.88839985631171 64-65 19.33264845701394 25.37533908092284 29.673273708806704 25.61873875325652 66-67 19.599417187594423 25.5265790657544 29.21850765109143 25.655496095559748 68-69 19.636262304768813 24.471213891462874 30.213663161570175 25.67886064219814 70-71 19.52643452804598 24.452777740475646 30.008258691769505 26.012529039708866 72-73 19.30586702834813 24.161373494299486 29.665825130594758 26.866934346757628 74-75 19.567727986893523 23.42816281037238 29.470436569218577 27.533672633515515 76-77 19.011441309573367 23.23613144077242 30.62564625941692 27.126780990237286 78-79 18.984919494541206 23.697409590825465 30.510494581492477 26.807176333140852 80-81 19.244625452005952 24.100268937244618 29.609285553604465 27.04582005714497 82-83 19.93406121160845 23.74333561634637 29.030189490082325 27.292413681962852 84-85 19.93446167586061 24.447943701505164 27.721166662749585 27.896427959884633 86-87 19.904782049603316 24.078793189700615 27.483271387946 28.533153372750075 88-89 19.92580157797549 23.147893234849757 27.88148396844049 29.044821218734263 90-91 20.796108095041447 22.47648976494801 27.44444332530913 29.28295881470141 92-93 21.92356632298483 21.674785884312058 26.749772538802695 29.65187525390041 94-95 21.471216354161072 22.06858109923172 26.049669585773383 30.410532960833827 96-97 21.82941364086552 21.49468701213678 25.77831495190613 30.89758439509157 98-99 22.242963669266437 21.531860317545583 25.30023804167912 30.924937971508864 100-101 22.08079987376069 20.97822036146075 24.985982736102763 31.9549970286758 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 202.0 1 129.5 2 134.0 3 195.5 4 125.0 5 89.0 6 144.0 7 273.0 8 389.0 9 381.0 10 299.5 11 261.5 12 258.0 13 263.0 14 277.0 15 256.5 16 250.5 17 270.5 18 283.5 19 322.0 20 369.0 21 415.0 22 532.5 23 629.0 24 723.5 25 883.0 26 1079.0 27 1358.5 28 1668.5 29 1997.5 30 2404.5 31 2730.0 32 2942.5 33 3254.5 34 3572.5 35 3642.5 36 3910.5 37 4480.0 38 4548.5 39 4085.0 40 3504.0 41 3269.5 42 3109.0 43 3222.0 44 3701.5 45 3504.0 46 3368.5 47 4022.0 48 4849.0 49 5258.0 50 5888.0 51 6188.0 52 5696.5 53 5597.0 54 5393.5 55 4627.5 56 3815.0 57 3418.0 58 3252.0 59 2814.0 60 2486.5 61 2228.5 62 1771.0 63 1413.0 64 1254.5 65 1168.5 66 890.0 67 636.5 68 520.0 69 399.0 70 298.5 71 228.5 72 179.0 73 194.5 74 241.0 75 183.5 76 90.0 77 61.0 78 49.5 79 40.5 80 34.5 81 30.0 82 26.5 83 19.5 84 14.5 85 12.5 86 10.5 87 6.5 88 5.5 89 4.5 90 3.5 91 2.0 92 1.5 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007385821907690655 2 0.008728698618179865 3 0.0 4 0.0 5 6.71438355244605E-4 6 0.003357191776223025 7 0.00402863013146763 8 0.003357191776223025 9 0.004700068486712235 10-11 0.00402863013146763 12-13 0.002014315065733815 14-15 0.0 16-17 0.0 18-19 3.357191776223025E-4 20-21 3.357191776223025E-4 22-23 0.0 24-25 6.71438355244605E-4 26-27 0.00134287671048921 28-29 0.0016785958881115125 30-31 0.008392979440557563 32-33 3.357191776223025E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0023500342433561174 48-49 0.002014315065733815 50-51 0.00134287671048921 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.357191776223025E-4 64-65 0.00134287671048921 66-67 6.71438355244605E-4 68-69 0.00537150684195684 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.009735856151046772 82-83 0.00537150684195684 84-85 0.009064417795802167 86-87 0.008392979440557563 88-89 0.0060429451972014445 90-91 0.006378664374823748 92-93 0.005035787664334537 94-95 0.020143150657338148 96-97 0.0043643493090899325 98-99 0.007050102730068352 100-101 0.007050102730068352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 148934.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.51861898559093 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22251681967322 33.31542831052681 2 6.7742683856427925 5.489679992479891 3 2.772346137275047 3.369949104972673 4 1.5941404566996984 2.58369479098124 5 1.138435024690949 2.3063907502652183 6 0.7954131176879992 1.9337424631044624 7 0.5866171742948993 1.663824244296131 8 0.45901965333244954 1.4879073952220445 9 0.3761641202399496 1.371748559764728 >10 2.812116793159447 22.75168866746344 >50 0.3115368044277997 8.670283481273584 >100 0.14913995956649984 10.472424026750105 >500 0.0033142213236999965 1.0373722588529148 >1k 0.004971331985549996 3.545865954046759 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2493 1.6738958196248002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1478 0.9923858890515261 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1310 0.8795842453704326 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 992 0.6660668484026482 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 553 0.37130541045026655 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 461 0.3095330817677629 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.3021472598600723 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 440 0.2954328763076262 No Hit GTACATGGGTGTGTGTGTGCAGCTTGATCGGGGCCATGGCCCAAACCCCT 409 0.27461828729504345 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 395 0.26521815032161894 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 386 0.25917520512441755 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 376 0.25246082157197147 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 335 0.22493184900694266 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.20613157506009375 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 297 0.19941719150764767 No Hit GTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGAG 286 0.19203136959995704 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 272 0.18263123262653255 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 250 0.16785958881115123 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 236 0.15845945183772678 No Hit GTCGTAGAGGACACAGGCGCCTTGTTCCCCGCAGAAGGTGCTCCAGAACA 224 0.1504021915747915 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 221 0.1483878765090577 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 212 0.14234493131185627 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 209 0.14033061624612245 No Hit CTATAAAACAGATTCCATGTTTTCACACCACTCATGGTCTTCCAGGTTAT 206 0.13831630118038862 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 205 0.13764486282514402 No Hit GTACATGGGGTGTTCCTTTGGTTGTTGGTTTTATCTCCCCCTCACTTTTT 199 0.1336162326936764 No Hit GGTATATTGGGGGGAAAAAAAGCAGTCTAATAAAATCCTAGACAGAGTTT 193 0.12958760256220878 No Hit CATCAGGACAGTCAGCCCTGAACTCAAGTCTTATGCGCTGGGAGTTCTTT 193 0.12958760256220878 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 192 0.12891616420696417 No Hit CTTTAACAGTGTGTTCCACGTATTCTTTTCCTGCCTGAATTACATCCAGG 187 0.12555897243074113 No Hit TCTTAATACCAGTCCAGAGTTCAACACGGCTGTTGTCCGAGCGCAGCAGC 180 0.1208589039440289 No Hit GTACATGGGATGGAGACGGTGGACAAGCGCGAGAAACTGGCGGAGGGCGC 179 0.12018746558878429 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 176 0.11817315052305048 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 175 0.11750171216780586 No Hit GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA 174 0.11683027381256127 No Hit GTGTAGAAATGCCAACAGCTGGGAAAGGGAGGGACCGCTATTTCAGTCAG 168 0.11280164368109363 No Hit CTCTACGACAATGTGGTCTACAGATACCTGTACGTCAGCATCGCCATTGC 166 0.11145876697060443 No Hit GGTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGA 164 0.11011589026011522 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 162 0.10877301354962601 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 161 0.10810157519438142 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 157 0.105415821773403 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.10407294506291377 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 155 0.10407294506291377 No Hit ACAGATACCTGTACGTCAGCATCGCCATTGCGCTCAAGTCCTTTGCCTTC 153 0.10273006835242456 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 153 0.10273006835242456 No Hit GTACATGGGAAGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 152 0.10205862999717996 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 150 0.10071575328669076 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.71438355244605E-4 0.0 10-11 0.0 0.0 0.0 0.00134287671048921 0.0 12-13 0.0 0.0 0.0 0.00268575342097842 0.0 14-15 0.0 0.0 0.0 0.005035787664334537 0.0 16-17 0.0 0.0 0.0 0.009064417795802167 0.0 18-19 0.0 0.0 0.0 0.0134287671048921 0.0 20-21 0.0 0.0 0.0 0.01880027394684894 0.0 22-23 0.0 0.0 0.0 0.034579075295097156 0.0 24-25 0.0 0.0 0.0 0.07520109578739576 0.0 26-27 0.0 0.0 0.0 0.14033061624612245 0.0 28-29 6.71438355244605E-4 0.0 0.0 0.254139417460083 0.0 30-31 6.71438355244605E-4 0.0 0.0 0.4874642459075832 0.0 32-33 6.71438355244605E-4 0.0 0.0 0.7956544509648569 0.0 34-35 6.71438355244605E-4 0.0 0.0 1.2891616420696415 0.0 36-37 6.71438355244605E-4 0.0 0.0 1.8494769495212644 0.0 38-39 6.71438355244605E-4 0.0 0.0 2.5786590033169055 0.0 40-41 6.71438355244605E-4 0.0 0.0 3.5854808170061907 0.0 42-43 6.71438355244605E-4 0.0 0.0 4.213275679159896 0.0 44-45 6.71438355244605E-4 0.0 0.0 4.657767870331825 0.0 46-47 6.71438355244605E-4 0.0 0.0 5.1365034176212285 0.0 48-49 6.71438355244605E-4 0.0 0.0 5.587038553990358 0.0 50-51 6.71438355244605E-4 0.0 0.0 6.021123450655995 0.0 52-53 6.71438355244605E-4 0.0 0.0 6.471994306202747 0.0 54-55 6.71438355244605E-4 0.0 0.0 6.8778787919481115 0.0 56-57 6.71438355244605E-4 0.0 0.0 7.243141257201176 0.0 58-59 6.71438355244605E-4 0.0 0.0 7.56308163347523 0.0 60-61 6.71438355244605E-4 0.0 0.0 7.890407831656976 0.0 62-63 6.71438355244605E-4 0.0 0.0 8.261377522929621 0.0 64-65 6.71438355244605E-4 0.0 0.0 8.60112533068339 0.0 66-67 6.71438355244605E-4 0.0 0.0 8.964373480870721 0.0 68-69 6.71438355244605E-4 0.0 0.0 9.407187076154539 0.0 70-71 6.71438355244605E-4 0.0 0.0 9.880886835779606 0.0 72-73 6.71438355244605E-4 0.0 0.0 10.35525803375992 0.0 74-75 6.71438355244605E-4 0.0 0.0 10.811836115326251 0.0 76-77 6.71438355244605E-4 0.0 0.0 11.264049847583493 0.0 78-79 6.71438355244605E-4 0.0 0.0 11.723985120926049 0.0 80-81 6.71438355244605E-4 0.0 0.0 12.202720668215452 0.0 82-83 6.71438355244605E-4 0.0 0.0 12.723756831885265 0.0 84-85 6.71438355244605E-4 0.0 0.0 13.2451287147327 0.0 86-87 6.71438355244605E-4 0.0 0.0 13.762471967448668 0.0 88-89 6.71438355244605E-4 0.0 0.0 14.308351350262534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTAGT 15 6.60717E-4 94.99765 7 ACTAGTC 20 0.0020707252 71.24824 8 CTTCTAT 25 0.0050132107 56.99859 1 AGTAATG 25 0.0050132107 56.99859 2 TTCTATC 35 2.780389E-4 54.28437 2 TGGACCG 35 2.780389E-4 54.28437 5 ATGTGGA 35 2.780389E-4 54.28437 2 GTGGACC 35 2.780389E-4 54.28437 4 AGGGGCC 35 2.780389E-4 54.28437 6 GGACCGT 35 2.780389E-4 54.28437 6 GTGGGTA 55 8.143652E-7 51.8169 9 GAGTAAT 40 5.373647E-4 47.498825 1 TCTTAGG 40 5.373647E-4 47.498825 2 GGGAGTG 70 7.860399E-8 47.498825 5 TAACCGG 20 5.382369E-4 47.498825 14-15 CGGGAGT 60 1.4837315E-6 47.498825 4 GGCCTTA 40 5.373647E-4 47.498825 9 AGTGGGT 65 2.5745812E-6 43.845066 8 GTCGGGA 65 2.5745812E-6 43.845066 2 ACTGATT 55 4.9431572E-5 43.180748 8 >>END_MODULE