FastQCFastQC Report
Fri 10 Feb 2017
SRR2568681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148934
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT66114.438878966522084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27821.867941504290491No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT27011.8135549975156782RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT20101.349591094041656RNA PCR Primer, Index 31 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18861.266332737991325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15371.032000752010958No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA8670.5821370539970725No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT7920.5317791773537272RNA PCR Primer, Index 31 (96% over 27bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG6880.4619495884082882RNA PCR Primer, Index 31 (96% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5660.3800341090684464No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.3491479447271946No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.319604657096432No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG4620.3102045201230075RNA PCR Primer, Index 38 (95% over 24bp)
GTACATGGGTGTGTGTGTGCAGCTTGATCGGGGCCATGGCCCAAACCCCT4430.29744719137336No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4230.2840184242684679No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4120.27663260236077725No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3390.2276176024279211No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.21553171203351817No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3190.214188835323029No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.2121745202572952No Hit
TCTTAATACCAGTCCAGAGTTCAACACGGCTGTTGTCCGAGCGCAGCAGC3010.2021029449286261No Hit
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC2980.20008862986289228RNA PCR Primer, Index 38 (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA2940.19740287644191387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.19270280795520164No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC2690.18061691756079873No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC2650.17793116413982032No Hit
GTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGAG2630.17658828742933114No Hit
CTATAAAACAGATTCCATGTTTTCACACCACTCATGGTCTTCCAGGTTAT2500.16785958881115123No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2400.16114520525870518No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2340.15711657512723756No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA2320.15577369841674835No Hit
GGTATATTGGGGGGAAAAAAAGCAGTCTAATAAAATCCTAGACAGAGTTT2310.15510226006150374No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2280.15308794499576994No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA2190.1470449997985685RNA PCR Primer, Index 31 (96% over 29bp)
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.1463735614433239No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT2050.13764486282514402RNA PCR Primer, Index 31 (95% over 21bp)
CATCAGGACAGTCAGCCCTGAACTCAAGTCTTATGCGCTGGGAGTTCTTT2040.1369734244698994No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1910.12824472585171956No Hit
CTTTAACAGTGTGTTCCACGTATTCTTTTCCTGCCTGAATTACATCCAGG1900.12757328749647495No Hit
GTACATGGGGTGTTCCTTTGGTTGTTGGTTTTATCTCCCCCTCACTTTTT1890.12690184914123034No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.12555897243074113No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1840.12354465736500733No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1840.12354465736500733No Hit
GTCGTAGAGGACACAGGCGCCTTGTTCCCCGCAGAAGGTGCTCCAGAACA1810.12153034229927351No Hit
CTTTTACAATGTCTTGCTGGAGGGCTGCCTCACAACTGAACCATCACTTC1670.11213020532584902No Hit
CTCTACGACAATGTGGTCTACAGATACCTGTACGTCAGCATCGCCATTGC1650.11078732861535984No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA1630.10944445190487061No Hit
GGTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGA1620.10877301354962601No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1610.10810157519438142No Hit
GCTTAAGTTTGGCCATGCACCATCATAAATGCTTCAACATCTCCAGGGAC1570.105415821773403No Hit
GTCTAGGGTCAGAGTAGAGGCAAAGAACTCACTGGTGCTCAGCCCGCCCT1560.10474438341815838No Hit
GAGTAGAGGCAAAGAACTCACTGGTGCTCAGCCCGCCCTCGTGGTTTTTG1550.10407294506291377No Hit
GTGTAGAAATGCCAACAGCTGGGAAAGGGAGGGACCGCTATTTCAGTCAG1530.10273006835242456No Hit
GTACATGGGGGCGCTCCCACCCAGCTGAATCTGGTTTAGGGGTTCCTCCC1510.10138719164193535No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1500.10071575328669076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGG201.7333063E-595.060822
CGGAGAT255.2348576E-576.074221
TATTGAC200.002070783471.2477347
TACTTAG301.2912693E-463.3951841
CTTAGTC301.2955727E-463.3525923
TGGACCG407.4998443E-659.3930555
TGGTGCC250.005006686357.0173344
GTGCCGC250.005006686357.0173346
ATGGGTG1200.055.433525
ACTTAGT352.7712696E-454.320472
TAGGGGC352.7758747E-454.3022235
TTAGTCT352.7758747E-454.3022234
ACAGTGC352.780486E-454.283998
CTTTACA405.34718E-447.5463871
TTAACAG707.840026E-847.5144425
GGACCGT502.8100014E-547.5144426
AACGCTG252.8224416E-547.4984936-37
CAGTGCG405.373834E-447.498499
GGCCTTA405.373834E-447.498499
TCAACCT205.3825567E-447.4984934-35
Produced by FastQC (version 0.11.5)