##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568681_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 148934 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.450904427464515 34.0 31.0 34.0 31.0 34.0 2 32.50765439724979 34.0 31.0 34.0 31.0 34.0 3 32.526864248593334 34.0 31.0 34.0 30.0 34.0 4 36.03411578282998 37.0 35.0 37.0 35.0 37.0 5 36.11678998751125 37.0 35.0 37.0 35.0 37.0 6 36.20167322438127 37.0 37.0 37.0 35.0 37.0 7 36.111814629298884 37.0 36.0 37.0 35.0 37.0 8 36.126841419689256 37.0 36.0 37.0 35.0 37.0 9 37.894067170693056 39.0 38.0 39.0 35.0 39.0 10-11 37.789497361247264 39.0 38.0 39.0 35.0 39.0 12-13 37.770492298602065 39.0 38.0 39.0 35.0 39.0 14-15 38.93979883706877 40.5 38.0 41.0 35.5 41.0 16-17 38.90847288060483 40.0 38.0 41.0 35.5 41.0 18-19 39.11577611559483 41.0 39.0 41.0 35.5 41.0 20-21 39.140501833026704 41.0 39.0 41.0 35.5 41.0 22-23 39.02165388695664 41.0 38.5 41.0 35.0 41.0 24-25 38.8950642566506 41.0 38.0 41.0 35.0 41.0 26-27 38.67129399599823 40.0 38.0 41.0 35.0 41.0 28-29 38.40949682409658 40.0 37.5 41.0 34.5 41.0 30-31 38.03565673385526 40.0 36.5 41.0 34.0 41.0 32-33 37.44335410315979 40.0 35.0 41.0 33.0 41.0 34-35 37.04246176158567 40.0 35.0 41.0 32.5 41.0 36-37 36.64978446828796 39.0 35.0 41.0 31.5 41.0 38-39 36.12785864879746 39.0 35.0 41.0 30.0 41.0 40-41 35.86091825909463 39.0 35.0 41.0 28.5 41.0 42-43 35.376321054963945 38.5 35.0 41.0 25.0 41.0 44-45 34.769199779768215 38.0 35.0 41.0 21.0 41.0 46-47 33.92991862167135 38.0 33.5 40.5 14.5 41.0 48-49 33.13992103884942 37.5 33.0 40.0 9.0 41.0 50-51 32.38393516591242 37.0 32.5 40.0 8.0 41.0 52-53 32.31828863792015 37.0 32.5 40.0 7.0 41.0 54-55 32.00656330992251 37.0 31.5 40.0 7.0 41.0 56-57 31.438852108987874 35.5 30.5 40.0 7.0 41.0 58-59 30.981139296601178 35.0 28.5 40.0 7.0 41.0 60-61 30.413203163817528 35.0 26.5 39.5 7.0 41.0 62-63 29.800354519451567 35.0 23.0 39.0 7.0 41.0 64-65 29.171357782641977 35.0 20.0 39.0 7.0 41.0 66-67 28.38224649844898 35.0 17.0 37.5 7.0 41.0 68-69 27.41328373642016 35.0 12.5 37.0 7.0 40.0 70-71 26.82707440879853 35.0 10.0 36.5 7.0 39.5 72-73 26.37411202277519 34.0 10.0 36.0 7.0 39.0 74-75 26.071937905380906 34.0 10.0 35.0 7.0 39.0 76-77 24.800179945479208 32.0 10.0 34.5 7.0 36.5 78-79 25.586162326936766 33.5 10.0 35.0 7.0 37.0 80-81 25.54362334994024 34.0 10.0 35.0 7.0 36.5 82-83 25.430318127492715 34.0 10.0 35.0 7.0 36.0 84-85 25.258047188687605 34.0 10.0 35.0 7.0 36.0 86-87 25.11066311251964 33.5 10.0 35.0 7.0 35.0 88-89 25.010833657861873 33.0 10.0 35.0 7.0 35.0 90-91 24.936038782279397 33.0 10.0 35.0 7.0 35.0 92-93 24.855929472115164 33.0 10.0 35.0 7.0 35.0 94-95 24.82869257523467 33.0 10.0 35.0 7.0 35.0 96-97 24.775121866061475 33.0 10.0 35.0 7.0 35.0 98-99 24.733331542831053 33.0 10.0 35.0 7.0 35.0 100-101 23.830411457424095 31.0 9.0 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48349352864472195 1101 2 0.3008527888859973 1101 3 0.48477496662250275 1101 4 0.23483976375773352 1101 5 0.24982559321809106 1101 6 0.33582820428416227 1101 7 0.3321719283726452 1101 8 0.22498639113458552 1101 9 0.18731424442123767 1101 10-11 0.19211096105625813 1101 12-13 0.2223489097013882 1101 14-15 0.6957068228991332 1101 16-17 0.41045194250122563 1101 18-19 0.6806836150560329 1101 20-21 0.8774612110011901 1101 22-23 0.5174911947299066 1101 24-25 0.1717769975873793 1101 26-27 0.330056258342843 1101 28-29 0.33566962869588934 1101 30-31 0.11062844381399373 1101 32-33 0.47740620264720945 1101 34-35 0.5017993095289341 1101 36-37 0.3483785118785434 1101 38-39 -0.09819223694872647 1101 40-41 0.026091678333486357 1101 42-43 0.20791172253446888 1101 44-45 0.09822168852927149 1101 46-47 0.12997976601215555 1101 48-49 0.440948880821189 1101 50-51 1.1836733872059213 1101 52-53 1.1055595484962524 1101 54-55 1.179998426228039 1101 56-57 2.0395393831865505 1101 58-59 1.5866534999166184 1101 60-61 1.1633135867306912 1101 62-63 1.6140149877749757 1101 64-65 1.4323183182281163 1101 66-67 1.48864781755681 1101 68-69 1.3773002518231081 1101 70-71 1.5881481904684023 1101 72-73 1.467999246495026 1101 74-75 1.5454311932205655 1101 76-77 1.5674431117060976 1101 78-79 1.440418027454136 1101 80-81 1.55362985176507 1101 82-83 0.8905854039187524 1101 84-85 0.5158723009580797 1101 86-87 0.8767864994283698 1101 88-89 1.0094646758004409 1101 90-91 0.5340025872478087 1101 92-93 1.2412227188535603 1101 94-95 1.0928483304919148 1101 96-97 0.7280584609391774 1101 98-99 0.8560608863383123 1101 100-101 0.7561675399167562 1102 1 0.3550711036121257 1102 2 0.09107443165000717 1102 3 -0.07376479869692787 1102 4 0.2613065173431366 1102 5 0.3300081225531599 1102 6 0.2141854402685155 1102 7 0.216787312988032 1102 8 0.15823280573693665 1102 9 0.0925293682804309 1102 10-11 0.047847597301370115 1102 12-13 0.26615595011859483 1102 14-15 0.47347735744932606 1102 16-17 0.08105168174503063 1102 18-19 0.18785441036241224 1102 20-21 0.3154664261250417 1102 22-23 0.3096685089411224 1102 24-25 0.41851754517538353 1102 26-27 0.5095217081472754 1102 28-29 0.6193723666359219 1102 30-31 0.7006545194332858 1102 32-33 0.7523736081230865 1102 34-35 1.2834811869735248 1102 36-37 1.183580597928092 1102 38-39 1.6647151946027705 1102 40-41 1.8034710785942423 1102 42-43 1.732683560865624 1102 44-45 1.8771656259477822 1102 46-47 1.3386499094280637 1102 48-49 1.532343926453521 1102 50-51 1.6335430091094452 1102 52-53 2.3644904481051228 1102 54-55 1.6373647886791431 1102 56-57 1.1887571146076752 1102 58-59 1.381959888443351 1102 60-61 1.2270684759093093 1102 62-63 0.9810945184138262 1102 64-65 1.0153039766375045 1102 66-67 1.0031849753143085 1102 68-69 0.9877304995414917 1102 70-71 1.4490999505727018 1102 72-73 1.389120498124754 1102 74-75 1.0295902544982702 1102 76-77 0.7974300738964502 1102 78-79 0.520209422499768 1102 80-81 0.7043469312957065 1102 82-83 0.5221369032668584 1102 84-85 0.5890796021314806 1102 86-87 1.0700068384114232 1102 88-89 0.3634412077430724 1102 90-91 0.33537155726084933 1102 92-93 0.3663205024259213 1102 94-95 0.45477792631981373 1102 96-97 0.5525043540291357 1102 98-99 1.2630361144999824 1102 100-101 0.52311669245913 1103 1 0.2156841747515088 1103 2 0.11409351782698707 1103 3 0.02039367440891482 1103 4 0.08860553411583538 1103 5 0.07899597680417259 1103 6 -0.11166765400563605 1103 7 0.15548019904933597 1103 8 0.015607010479563144 1103 9 0.12977633184318194 1103 10-11 0.2273154978219054 1103 12-13 0.22700036885775177 1103 14-15 -0.063596095413601 1103 16-17 -0.09633409037758156 1103 18-19 0.055986278494287944 1103 20-21 -0.05636700360312119 1103 22-23 -0.11612674843308213 1103 24-25 -0.34536331081073257 1103 26-27 -0.22604798531715886 1103 28-29 -0.09401687570068873 1103 30-31 -0.032196839733870775 1103 32-33 0.08779870933766176 1103 34-35 -0.3053562913376382 1103 36-37 -0.905603901782726 1103 38-39 -0.9940760141884368 1103 40-41 -1.2844410093178453 1103 42-43 -0.9704685501812236 1103 44-45 -0.7630310195120202 1103 46-47 -0.5295397956037462 1103 48-49 -0.35903837545509987 1103 50-51 -1.3860347815209764 1103 52-53 -0.9671752546132169 1103 54-55 -0.9078058301756826 1103 56-57 -1.0027697795507962 1103 58-59 -0.7311112509435773 1103 60-61 -1.5463843870172376 1103 62-63 -1.4271472398279315 1103 64-65 -0.5931691292040249 1103 66-67 -0.4700943769124102 1103 68-69 -0.8806327161901457 1103 70-71 -0.7029821778252128 1103 72-73 -0.45356313403256365 1103 74-75 -0.7229834875491541 1103 76-77 -0.4917833442643378 1103 78-79 -0.2572052680728163 1103 80-81 -0.4201180773798292 1103 82-83 -0.6328949070281062 1103 84-85 -0.1832165227962257 1103 86-87 -0.7705946653479785 1103 88-89 -0.541127907352358 1103 90-91 -0.25473833284904046 1103 92-93 -0.04108262076667302 1103 94-95 -0.27321513325219016 1103 96-97 0.06337769121826042 1103 98-99 0.3899591914230598 1103 100-101 0.20920691802303892 1104 1 0.18860475745827898 1104 2 -0.1332044145038367 1104 3 -0.14419749100461843 1104 4 0.03979209426621111 1104 5 0.08721966101470002 1104 6 -0.13821840588533263 1104 7 0.1056094283726452 1104 8 0.2648433826600112 1104 9 0.374019752895812 1104 10-11 0.29201827037829275 1104 12-13 0.10708143088782407 1104 14-15 -0.2221957194737456 1104 16-17 0.24559495097579997 1104 18-19 0.11904431420857264 1104 20-21 0.3035072915096606 1104 22-23 0.06946418201804505 1104 24-25 0.17813292979077033 1104 26-27 -0.11505761877580056 1104 28-29 0.24635553971284452 1104 30-31 0.6119525963563603 1104 32-33 -0.08674898803075592 1104 34-35 -0.010449101488013923 1104 36-37 -0.48901555591806556 1104 38-39 -0.5950804784741521 1104 40-41 -0.6577669021749841 1104 42-43 -0.4833621122112959 1104 44-45 -0.8538439894368324 1104 46-47 -0.7891145136488618 1104 48-49 -0.8827402081618629 1104 50-51 -1.1237286255059402 1104 52-53 -1.3159182057410348 1104 54-55 -1.422490980551622 1104 56-57 -1.6408101930846328 1104 58-59 -2.0227877077104957 1104 60-61 -1.4272584471676133 1104 62-63 -1.760852596970789 1104 64-65 -1.9316912156701882 1104 66-67 -2.3434496400703075 1104 68-69 -2.6281949812277396 1104 70-71 -2.2971139917349888 1104 72-73 -2.0570934441829394 1104 74-75 -2.2628977262709604 1104 76-77 -2.0308752145650892 1104 78-79 -1.5936126928848466 1104 80-81 -2.0701122033196775 1104 82-83 -2.5696900198100643 1104 84-85 -2.305631936330059 1104 86-87 -1.5651494115885782 1104 88-89 -1.7417270614876976 1104 90-91 -2.0019481542776134 1104 92-93 -2.01162420911254 1104 94-95 -2.0513518813724936 1104 96-97 -2.0735172805862483 1104 98-99 -1.9186345585769402 1104 100-101 -2.00813330754087 1105 1 0.4363366122969907 1105 2 0.29169477904454766 1105 3 0.16779847673731751 1105 4 0.3172618523307307 1105 5 0.17492127391792422 1105 6 0.21157998121144317 1105 7 0.25959128321135694 1105 8 0.3190268504019471 1105 9 0.4077899141861323 1105 10-11 0.34431161715248493 1105 12-13 0.32192518088782407 1105 14-15 0.28687686117142164 1105 16-17 0.4817340638790242 1105 18-19 0.4586360480795406 1105 20-21 -0.01189089397421128 1105 22-23 -0.0027384389496987183 1105 24-25 0.3757135749520586 1105 26-27 0.13985669574032755 1105 28-29 0.016642838099940604 1105 30-31 -0.2877449842888069 1105 32-33 -0.18087701222430752 1105 34-35 -0.3283674482622061 1105 36-37 -0.21381394301483425 1105 38-39 -0.9825552768612482 1105 40-41 -0.7418142812072404 1105 42-43 -0.7105546525338724 1105 44-45 -0.5922512475013448 1105 46-47 -0.5045883039714454 1105 48-49 -0.2611424259037989 1105 50-51 -0.6591368916349722 1105 52-53 -0.7748395767087786 1105 54-55 -1.2334788837774298 1105 56-57 -1.2995803543749567 1105 58-59 -1.6335487964201754 1105 60-61 -1.5129439310385813 1105 62-63 -1.159290096970789 1105 64-65 -1.4092617801863163 1105 66-67 -1.9734899626509517 1105 68-69 -2.2946516344535475 1105 70-71 -2.196433548186601 1105 72-73 -2.1862517103119714 1105 74-75 -1.8439209117548323 1105 76-77 -1.2999024323070252 1105 78-79 -1.5903364832074267 1105 80-81 -1.5084942597712896 1105 82-83 -1.8307787294874842 1105 84-85 -1.931640000846187 1105 86-87 -1.8848318712659982 1105 88-89 -1.3170798840683453 1105 90-91 -1.1527798075034212 1105 92-93 -1.2516695720157642 1105 94-95 -1.2940434136305576 1105 96-97 -1.0232400628443123 1105 98-99 -1.448750485996296 1105 100-101 -0.9446252430247419 1106 1 0.12762229342950349 1106 2 0.11398533369759889 1106 3 0.16054621403135627 1106 4 -0.08335313954673751 1106 5 0.07710275453987947 1106 6 -0.07369502459037136 1106 7 -0.031137424145342152 1106 8 0.05098322078950446 1106 9 -0.1818480528595856 1106 10-11 -0.17080209832674598 1106 12-13 -0.393002876666138 1106 14-15 0.1260506834039532 1106 16-17 0.13301626392544108 1106 18-19 -0.5072202991007799 1106 20-21 -0.30583960597235205 1106 22-23 -0.37660708596757075 1106 24-25 -0.4072987248854858 1106 26-27 -0.2602071241714867 1106 28-29 -0.23416941532313018 1106 30-31 -0.06846556911126811 1106 32-33 -0.6444996624911852 1106 34-35 -0.4931098478908922 1106 36-37 -0.5688267069972 1106 38-39 0.2736526600150526 1106 40-41 -0.024420454333252906 1106 42-43 -0.27492573631201367 1106 44-45 0.05386734149842454 1106 46-47 0.5191701086532916 1106 48-49 0.9232681101834572 1106 50-51 0.7392153942782258 1106 52-53 1.077910481309324 1106 54-55 1.1371987676498136 1106 56-57 1.2936547349729182 1106 58-59 1.5609858984046134 1106 60-61 1.6039915528323867 1106 62-63 1.7696667375615824 1106 64-65 1.7578883706607478 1106 66-67 1.1014694246778944 1106 68-69 1.063436088916145 1106 70-71 0.5808929776894729 1106 72-73 0.8874602878314519 1106 74-75 1.0887490812829945 1106 76-77 0.7298318447874301 1106 78-79 0.5152564617914095 1106 80-81 0.9477891006371557 1106 82-83 0.45841249817554797 1106 84-85 0.9873087111519538 1106 86-87 0.9160500038790502 1106 88-89 0.9143543234043889 1106 90-91 1.1918187072331783 1106 92-93 1.6177316811752291 1106 94-95 1.0269417337354234 1106 96-97 1.0081767391979248 1106 98-99 0.9906786158834926 1106 100-101 0.5968576996533734 1107 1 0.1551757608596418 1107 2 0.23403623175466493 1107 3 0.08018601239674439 1107 4 0.21714115889206198 1107 5 -0.002278638305028835 1107 6 0.11497036962487073 1107 7 0.2131243093250248 1107 8 0.03923751531307573 1107 9 0.1655801270454731 1107 10-11 0.051876901330672354 1107 12-13 0.0846802829286375 1107 14-15 0.1190032601180917 1107 16-17 0.2475613625404236 1107 18-19 0.07299308121537251 1107 20-21 0.22200750409469094 1107 22-23 0.6062148197731503 1107 24-25 0.4919084399948517 1107 26-27 0.565931964557528 1107 28-29 0.5774833033182887 1107 30-31 0.438802086152279 1107 32-33 0.14329459190121696 1107 34-35 0.6477407369473624 1107 36-37 0.569870498503704 1107 38-39 0.8528627613217665 1107 40-41 0.3584161335393006 1107 42-43 0.0017835085370023762 1107 44-45 -0.4164502819538356 1107 46-47 0.03598114961643972 1107 48-49 0.5382047323143269 1107 50-51 0.6193357877593613 1107 52-53 0.14363749153788063 1107 54-55 0.16201157046878478 1107 56-57 0.4494321538541399 1107 58-59 0.16165638242555858 1107 60-61 0.18562420589361395 1107 62-63 0.5456418758183261 1107 64-65 0.12809832514613717 1107 66-67 0.6673921966643874 1107 68-69 0.0719272551668162 1107 70-71 0.2748194691493637 1107 72-73 0.1934114452728437 1107 74-75 0.11786864277665998 1107 76-77 -0.04817432170794689 1107 78-79 -0.17646770989165006 1107 80-81 -0.5210847798502876 1107 82-83 0.12865181692463068 1107 84-85 0.06830513849987341 1107 86-87 -0.200061188799463 1107 88-89 0.18944853375039727 1107 90-91 0.03097595286524424 1107 92-93 0.46464598646569044 1107 94-95 0.02735772747104548 1107 96-97 0.2964603979851823 1107 98-99 -0.14405441401911645 1107 100-101 -0.6894683415544769 1108 1 0.33170206758486387 1108 2 -0.13102878159244113 1108 3 -0.02809780746031265 1108 4 0.014178961354815556 1108 5 -0.061910085820741756 1108 6 -0.029436760315711297 1108 7 0.05369091571441942 1108 8 -0.30408462366910527 1108 9 -0.43551347495228754 1108 10-11 -0.06325269481158102 1108 12-13 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1.0 12 2.0 13 13.0 14 41.0 15 209.0 16 817.0 17 1952.0 18 2816.0 19 3203.0 20 4364.0 21 5653.0 22 5605.0 23 4247.0 24 4067.0 25 4138.0 26 4575.0 27 5244.0 28 5406.0 29 4102.0 30 2492.0 31 2143.0 32 2546.0 33 3172.0 34 4427.0 35 7548.0 36 14245.0 37 26913.0 38 23650.0 39 5337.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.733850875366834 18.07748356378728 12.284683934483477 21.90398162636241 2 13.540910435320372 21.884326853270952 43.334408862359176 21.2403538490495 3 18.357796070742747 33.77939221400083 32.34184269542214 15.520969019834288 4 10.44556649254032 21.033477916392496 31.959794271287954 36.56116131977923 5 9.234963138034297 41.649992614178096 33.890179542616195 15.224864705171418 6 23.654065668434836 37.44510844020681 18.55032565634862 20.350500235009736 7 21.476541617976924 37.50184685229211 18.959449839491747 22.06216169023922 8 27.075751675238696 31.629446600507606 21.648515449796555 19.646286274457143 9 28.991365302751554 13.556340392388574 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20.50774168423597 34-35 17.622571071749903 27.54407992802181 34.247384747606326 20.585964252621967 36-37 16.73090093598507 29.53590180885493 34.447137658291595 19.286059596868412 38-39 18.094256516309237 25.865819759087916 34.22959163119234 21.810332093410505 40-41 17.09582768206051 27.43094256516309 34.11678998751125 21.35643976526515 42-43 19.831603260504654 26.294533148911597 33.86533632347214 20.008527267111607 44-45 16.55196261431238 28.694253830555816 34.02245289859938 20.731330656532425 46-47 19.60096418547813 25.96250688224314 31.52235218284609 22.914176749432634 48-49 18.589442303302135 25.536814965018063 31.848671223494975 24.025071508184833 50-51 17.115635113540225 25.805390307115907 33.623954234761705 23.455020344582163 52-53 16.99511192877382 26.570158593739507 34.689526904534894 21.745202572951776 54-55 16.17763573126351 27.077430271126808 30.808613211221076 25.9363207863886 56-57 19.000698295889453 27.943250030214728 27.32619818174493 25.729853492150884 58-59 16.28943021741174 28.668067734701275 31.38974310768528 23.6527589402017 60-61 18.11339250943371 31.20744759423637 26.982421743859696 23.696738152470225 62-63 15.774825677231785 33.33937629881793 26.860981579026884 24.024816444923406 64-65 16.06488802049237 32.485639563966465 26.79887332265743 24.650599092883738 66-67 16.557334121154337 32.2585843393718 26.386184484402484 24.797897055071374 68-69 17.31572374340312 29.84308485637934 27.718653900385405 25.12253749983214 70-71 17.46612593497791 29.276726603730513 27.779419071535045 25.47772838975654 72-73 17.654128674446397 28.320598385862194 27.306055031087595 26.71921790860381 74-75 18.213436824365157 26.8018048262989 27.202317805202302 27.782440544133642 76-77 17.33821692830381 26.366041333745148 29.29250540507876 27.003236332872277 78-79 17.44598278432057 26.16494554634939 28.840291672821515 27.54877999650852 80-81 17.777001893456163 26.215303422992736 28.460593282930695 27.54710140062041 82-83 18.110706756012732 26.095787395759196 27.922771160379767 27.87073468784831 84-85 18.49376233767978 26.03099359447809 26.203888970953574 29.271355096888556 86-87 17.996226516443524 25.616380410114548 26.200531779177354 30.18686129426457 88-89 17.912632441215575 25.01309304792727 26.20993191615078 30.86434259470638 90-91 18.34369586528261 24.190581062752628 26.007828971222153 31.45789410074261 92-93 18.513905488337116 23.35531174882834 25.803040272872547 32.327742489962 94-95 18.065384667033722 23.619858460794717 25.07251534236642 33.24224152980515 96-97 18.029798434205755 23.14011575597244 25.261860958545395 33.568224851276405 98-99 18.81202411806572 23.286489317415768 24.864369452240588 33.03711711227792 100-101 18.700565351095115 23.217666886003197 24.283575274954007 33.79819248794768 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 167.0 1 111.0 2 113.0 3 197.0 4 143.5 5 84.5 6 145.0 7 274.5 8 404.5 9 404.0 10 308.5 11 240.5 12 228.0 13 223.0 14 232.5 15 242.5 16 250.0 17 275.5 18 283.0 19 306.5 20 352.0 21 402.5 22 443.0 23 514.5 24 619.0 25 717.5 26 834.5 27 1038.5 28 1311.5 29 1603.0 30 1954.0 31 2306.5 32 2577.0 33 2980.5 34 3441.0 35 3647.5 36 4108.5 37 4809.0 38 5017.0 39 4762.5 40 4389.5 41 4400.5 42 4373.5 43 4464.0 44 4872.5 45 4628.5 46 4307.0 47 4661.5 48 5319.5 49 5592.0 50 6144.0 51 6744.0 52 6190.5 53 5274.5 54 4712.5 55 4024.5 56 3262.0 57 2921.5 58 2764.5 59 2439.0 60 2038.0 61 1771.0 62 1340.0 63 876.5 64 750.5 65 723.0 66 522.0 67 350.5 68 305.5 69 204.0 70 129.0 71 88.0 72 68.0 73 71.0 74 85.5 75 78.5 76 35.5 77 9.0 78 6.5 79 3.0 80 1.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016785958881115125 2 0.11414452039158285 3 0.0 4 0.0 5 0.0 6 0.00268575342097842 7 0.02148602736782736 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0010071575328669076 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 3.357191776223025E-4 64-65 3.357191776223025E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 148934.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.45465776787033 #Duplication Level Percentage of deduplicated Percentage of total 1 75.20744050332817 22.152094216230008 2 9.455639646211361 5.570252595109243 3 3.9504878271177173 3.4908080089167015 4 2.206619859578736 2.5998093115071104 5 1.522750068386979 2.2426041065169806 6 1.0691164402297801 1.8894275316583184 7 0.7841706938998815 1.6168235594290088 8 0.599525850278107 1.412706299434649 9 0.5060636454819002 1.3415338337787208 >10 4.016595240266254 23.54935743349403 >50 0.44679493024528133 8.99928827534344 >100 0.2097200692988055 11.061946902654867 >500 0.011397829853195951 2.305047873554729 >1k 0.011397829853195951 7.329421085850107 >5k 0.00227956597063919 4.438878966522084 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 6611 4.438878966522084 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 1.867941504290491 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 2701 1.8135549975156782 RNA PCR Primer, Index 31 (95% over 22bp) ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 2010 1.349591094041656 RNA PCR Primer, Index 31 (95% over 24bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1886 1.266332737991325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1537 1.032000752010958 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 867 0.5821370539970725 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 792 0.5317791773537272 RNA PCR Primer, Index 31 (96% over 27bp) TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 688 0.4619495884082882 RNA PCR Primer, Index 31 (96% over 29bp) GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 566 0.3800341090684464 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.3491479447271946 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 476 0.319604657096432 No Hit CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG 462 0.3102045201230075 RNA PCR Primer, Index 38 (95% over 24bp) GTACATGGGTGTGTGTGTGCAGCTTGATCGGGGCCATGGCCCAAACCCCT 443 0.29744719137336 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 423 0.2840184242684679 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 412 0.27663260236077725 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 339 0.2276176024279211 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 321 0.21553171203351817 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 319 0.214188835323029 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 316 0.2121745202572952 No Hit TCTTAATACCAGTCCAGAGTTCAACACGGCTGTTGTCCGAGCGCAGCAGC 301 0.2021029449286261 No Hit ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC 298 0.20008862986289228 RNA PCR Primer, Index 38 (96% over 25bp) ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 294 0.19740287644191387 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 287 0.19270280795520164 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC 269 0.18061691756079873 No Hit TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 265 0.17793116413982032 No Hit GTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGAG 263 0.17658828742933114 No Hit CTATAAAACAGATTCCATGTTTTCACACCACTCATGGTCTTCCAGGTTAT 250 0.16785958881115123 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 240 0.16114520525870518 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 234 0.15711657512723756 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 232 0.15577369841674835 No Hit GGTATATTGGGGGGAAAAAAAGCAGTCTAATAAAATCCTAGACAGAGTTT 231 0.15510226006150374 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 228 0.15308794499576994 No Hit CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA 219 0.1470449997985685 RNA PCR Primer, Index 31 (96% over 29bp) GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 218 0.1463735614433239 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 205 0.13764486282514402 RNA PCR Primer, Index 31 (95% over 21bp) CATCAGGACAGTCAGCCCTGAACTCAAGTCTTATGCGCTGGGAGTTCTTT 204 0.1369734244698994 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 191 0.12824472585171956 No Hit CTTTAACAGTGTGTTCCACGTATTCTTTTCCTGCCTGAATTACATCCAGG 190 0.12757328749647495 No Hit GTACATGGGGTGTTCCTTTGGTTGTTGGTTTTATCTCCCCCTCACTTTTT 189 0.12690184914123034 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 187 0.12555897243074113 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 184 0.12354465736500733 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 184 0.12354465736500733 No Hit GTCGTAGAGGACACAGGCGCCTTGTTCCCCGCAGAAGGTGCTCCAGAACA 181 0.12153034229927351 No Hit CTTTTACAATGTCTTGCTGGAGGGCTGCCTCACAACTGAACCATCACTTC 167 0.11213020532584902 No Hit CTCTACGACAATGTGGTCTACAGATACCTGTACGTCAGCATCGCCATTGC 165 0.11078732861535984 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA 163 0.10944445190487061 No Hit GGTATTAAGAAGGGCCCTCCACGAAAGCTCAAATTTCCTGAGCCTCAAGA 162 0.10877301354962601 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 161 0.10810157519438142 No Hit GCTTAAGTTTGGCCATGCACCATCATAAATGCTTCAACATCTCCAGGGAC 157 0.105415821773403 No Hit GTCTAGGGTCAGAGTAGAGGCAAAGAACTCACTGGTGCTCAGCCCGCCCT 156 0.10474438341815838 No Hit GAGTAGAGGCAAAGAACTCACTGGTGCTCAGCCCGCCCTCGTGGTTTTTG 155 0.10407294506291377 No Hit GTGTAGAAATGCCAACAGCTGGGAAAGGGAGGGACCGCTATTTCAGTCAG 153 0.10273006835242456 No Hit GTACATGGGGGCGCTCCCACCCAGCTGAATCTGGTTTAGGGGTTCCTCCC 151 0.10138719164193535 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 150 0.10071575328669076 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.71438355244605E-4 0.0 10-11 0.0 0.0 0.0 6.71438355244605E-4 0.0 12-13 0.0 0.0 0.0 0.002014315065733815 0.0 14-15 0.0 0.0 0.0 0.004700068486712235 0.0 16-17 0.0 0.0 0.0 0.009064417795802167 0.0 18-19 0.0 0.0 0.0 0.015107362993003613 0.0 20-21 0.0 0.0 0.0 0.02148602736782736 0.0 22-23 0.0 0.0 0.0 0.03625767118320867 0.0 24-25 0.0 0.0 0.0 0.07922972591886339 0.0 26-27 0.0 0.0 0.0 0.1450306847328347 0.0 28-29 0.0 0.0 0.0 0.2591752051244175 0.0 30-31 0.0 0.0 0.0 0.4911571568614285 0.0 32-33 0.0 0.0 0.0 0.7976687660305908 0.0 34-35 0.0 0.0 0.0 1.2911759571353754 0.0 36-37 0.0 0.0 0.0 1.853505579652732 0.0 38-39 0.0 0.0 0.0 2.578994722494528 0.0 40-41 0.0 0.0 0.0 3.580445029341856 0.0 42-43 0.0 0.0 0.0 4.199511192877383 0.0 44-45 0.0 0.0 0.0 4.641989068983577 0.0 46-47 0.0 0.0 0.0 5.118374582029624 0.0 48-49 0.0 0.0 0.0 5.56689540333302 0.0 50-51 0.0 0.0 0.0 5.999637423288168 0.0 52-53 0.0 0.0 0.0 6.444801052815341 0.0 54-55 0.0 0.0 0.0 6.851692696093572 0.0 56-57 0.0 0.0 0.0 7.215612284636148 0.0 58-59 0.0 0.0 0.0 7.53387406502209 0.0 60-61 0.0 0.0 0.0 7.859857386493346 0.0 62-63 0.0 0.0 0.0 8.236870022963192 0.0 64-65 0.0 0.0 0.0 8.580310741670807 0.0 66-67 0.0 0.0 0.0 8.943223172680517 0.0 68-69 0.0 0.0 0.0 9.390401117273424 0.0 70-71 0.0 0.0 0.0 9.867793787852337 0.0 72-73 0.0 0.0 0.0 10.341157828299785 0.0 74-75 0.0 0.0 0.0 10.79404299891227 0.0 76-77 0.0 0.0 0.0 11.2445781352814 0.0 78-79 0.0 0.0 0.0 11.700820497670108 0.0 80-81 0.0 0.0 0.0 12.17619885318329 0.0 82-83 0.0 0.0 0.0 12.696563578497859 0.0 84-85 0.0 0.0 0.0 13.219949776411028 0.0 86-87 0.0 0.0 0.0 13.73930734419273 0.0 88-89 0.0 0.0 0.0 14.291229672203794 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTAGG 20 1.7333063E-5 95.06082 2 CGGAGAT 25 5.2348576E-5 76.07422 1 TATTGAC 20 0.0020707834 71.247734 7 TACTTAG 30 1.2912693E-4 63.395184 1 CTTAGTC 30 1.2955727E-4 63.352592 3 TGGACCG 40 7.4998443E-6 59.393055 5 TGGTGCC 25 0.0050066863 57.017334 4 GTGCCGC 25 0.0050066863 57.017334 6 ATGGGTG 120 0.0 55.43352 5 ACTTAGT 35 2.7712696E-4 54.32047 2 TAGGGGC 35 2.7758747E-4 54.302223 5 TTAGTCT 35 2.7758747E-4 54.302223 4 ACAGTGC 35 2.780486E-4 54.28399 8 CTTTACA 40 5.34718E-4 47.546387 1 TTAACAG 70 7.840026E-8 47.514442 5 GGACCGT 50 2.8100014E-5 47.514442 6 AACGCTG 25 2.8224416E-5 47.49849 36-37 CAGTGCG 40 5.373834E-4 47.49849 9 GGCCTTA 40 5.373834E-4 47.49849 9 TCAACCT 20 5.3825567E-4 47.49849 34-35 >>END_MODULE