FastQCFastQC Report
Fri 10 Feb 2017
SRR2568680_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568680_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331091
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50500.034.801821
TCAACGC49700.033.547134
ATCAACG50700.032.603893
GGTATCA30950.032.5361981
CAACGCA51300.032.4082345
AACGCAG52800.031.5775096
ACGCAGA61400.027.0776427
CGCAGAG62100.026.3134658
TATCAAC65300.025.6775232
GTACATG90150.025.5545141
ACATGGG89350.023.072433
TACATGG99550.021.4715232
GTATAGG16200.021.1110131
GCAGAGT80000.019.7726759
CATGGGG58250.019.2451254
TTAATAC29000.018.672613
TAATACT30900.018.2933464
AGAGTAC68300.017.31677410-11
GTATTAT17350.016.1526621
GGACCGA2951.2316978E-616.1021046
Produced by FastQC (version 0.11.5)