FastQCFastQC Report
Fri 10 Feb 2017
SRR2568680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331091
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52600.032.483751
TCAACGC50650.031.616194
GGTATCA32850.030.7155271
ATCAACG52650.030.4151973
CAACGCA53000.030.1246815
AACGCAG54550.029.3558226
ACGCAGA61800.025.7570367
CGCAGAG62300.025.4637248
TATCAAC65650.025.15172
GTACATG86300.023.7145631
ACATGGG81250.022.9256553
TACATGG94350.020.8800262
TATACCG2351.1289376E-720.220475
GCAGAGT79900.019.616919
GTATAGG16550.018.1175731
AGAGTAC69250.016.90676910-11
CATGGGG50850.016.7271214
GAGTACA68250.016.2484051
GGACCGT2154.776981E-415.4710116
TAATACT32300.015.4470614
Produced by FastQC (version 0.11.5)