FastQCFastQC Report
Fri 10 Feb 2017
SRR2568678_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568678_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165665
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG55270.47414994874170535No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC51110.4384621653734134No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT41340.35464734722240093No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT39690.340492336992189No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG36370.3120107406501868No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC36120.3098660421304577No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT33890.2907353313344743No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA33700.2891053604594802No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA32570.27941132315030476No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT31900.27366353111743086No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA31620.27126146877533425No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG31470.2699746496634968No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA30670.26311161440036374No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC28580.24518193477542863No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT27390.23497316982151817No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG25930.22244813046630035No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC25850.22176182693998706No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT25820.22150446311761957No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG25800.22133288723604122No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT25630.21987449224262545No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC25070.2150703675584323No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA24540.21052360669660666No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC24540.21052360669660666No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC24530.2104378187558175No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG24520.21035203081502832No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC24030.20614842171635933No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG23080.19799856734138882No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG22690.19465283765061145No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG22510.1931086547164065No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG22210.19053501649273163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.18187043447302612No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC20640.17706630978883298No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT20280.1739779439204231No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG20150.172862700690164No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT19620.16831593982833834No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT19580.16797278806518168No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT19530.16754384836123584No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT19300.1655707257230851No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA18960.16265393573625356No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG18040.15476144518365054No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA17230.14781262197972833No Hit
CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG16930.1452389837560534No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG16810.14420952846658344No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA16370.14043485907186026No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT15770.1352875826245105No Hit
CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT15560.13348603586793806No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC15210.13048345794031735No Hit
CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC15090.12945400265084736No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT14970.1284245473613774No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG14560.12490724178902171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.12233360356534682No Hit
CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA14180.12164730003903351No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC14100.1209609965127202No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG14030.12036048092719606No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA13900.11924523769693694No Hit
ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT13510.11589950800615957No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA13450.11538478036142458No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG13260.11375480948643049No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT13200.11324008184169551No Hit
GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA13140.11272535419696054No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC13110.11246799037459304No Hit
ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA12960.1111811712627556No Hit
CATTTATAGAAACCTGAAACATTGGAGTACTTCTACTGTTCGCAGTCATA12940.11100959538117726No Hit
ATATGGTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGAT12910.11075223155880978No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG12760.10946541244697232No Hit
CACTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCG12730.10920804862460484No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA12640.10843595715750237No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT12630.10835016921671321No Hit
TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC12620.10826438127592404No Hit
CTTCTGAGCGTTCTATCATTCTAGCCTCGTACCAACACATGATCTAGGAG12390.10629125863777329No Hit
CTATCATTCTAGCCTCGTACCAACACATGATCTAGGAGGCTGCTGACCTC12360.10603389481540579No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC12170.1044039239404117No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA12160.10431813599962253No Hit
GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT12120.1039749842364659No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC11950.1025165892430501No Hit
ATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGCCA11950.1025165892430501No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA11940.10243080130226095No Hit
ATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAG11760.10088661836805601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT4250.072.641581
ACATATA5250.058.8050882
TGCATAG250.00502279356.99572
AAAGACA752.386514E-950.6671944
CATATAG6400.048.9827843
GATTATC502.8268465E-547.5004929
GGCTCGG953.8562575E-1045.000479
ATGGGCG1300.043.8466075
GGTATCA14850.043.8182451
GGGAGAC554.9630515E-543.1822667
GGGATAC554.9630515E-543.1822667
CATTCCG1253.6379788E-1241.8004349
TGGGTAT4150.041.2052466
GCGGCTC1051.027729E-940.714717
TACCTGG1051.027729E-940.7112162
GTATCAA32800.040.4009171
ATATGGA1900.040.0004164
GGATGAC9950.039.6235288
GGGCCTA853.6404708E-739.1180537
GTACATG61400.038.909931
Produced by FastQC (version 0.11.5)