##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568678_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1165665 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33364131204077 34.0 31.0 34.0 31.0 34.0 2 32.46281221448701 34.0 31.0 34.0 31.0 34.0 3 32.483057310633846 34.0 31.0 34.0 31.0 34.0 4 35.855440456734996 37.0 35.0 37.0 35.0 37.0 5 35.90025350336503 37.0 37.0 37.0 35.0 37.0 6 36.026370355119184 37.0 37.0 37.0 35.0 37.0 7 36.003778100912356 37.0 37.0 37.0 35.0 37.0 8 36.019088674704996 37.0 37.0 37.0 35.0 37.0 9 37.85486910904934 39.0 39.0 39.0 35.0 39.0 10-11 37.81568203557626 39.0 39.0 39.0 35.0 39.0 12-13 37.828313451978055 39.0 39.0 39.0 35.0 39.0 14-15 39.26350366528977 41.0 39.0 41.0 36.0 41.0 16-17 39.24517206916224 41.0 39.0 41.0 36.0 41.0 18-19 39.28829809593665 41.0 39.5 41.0 36.0 41.0 20-21 39.3080880870576 41.0 40.0 41.0 36.0 41.0 22-23 39.23824769552144 41.0 39.5 41.0 36.0 41.0 24-25 39.15714291841996 41.0 39.0 41.0 36.0 41.0 26-27 39.06901854306341 41.0 39.0 41.0 36.0 41.0 28-29 38.97034010629126 41.0 39.0 41.0 35.0 41.0 30-31 38.87594463246302 41.0 39.0 41.0 35.0 41.0 32-33 38.74750979054875 40.5 38.5 41.0 35.0 41.0 34-35 38.660235573685405 40.0 38.0 41.0 35.0 41.0 36-37 38.55169881569748 40.0 38.0 41.0 34.5 41.0 38-39 38.445391257350956 40.0 38.0 41.0 34.0 41.0 40-41 38.371065443330636 40.0 38.0 41.0 34.0 41.0 42-43 38.227060948042535 40.0 38.0 41.0 34.0 41.0 44-45 38.04885065606328 40.0 38.0 41.0 33.0 41.0 46-47 38.11787091488549 40.0 38.0 41.0 33.5 41.0 48-49 38.24631776711148 40.0 38.0 41.0 34.0 41.0 50-51 37.162077440774155 39.0 36.5 40.0 32.5 40.5 52-53 37.34218793564189 39.5 37.5 40.0 33.0 40.5 54-55 37.9918904659572 40.0 38.0 41.0 33.0 41.0 56-57 38.02545714248948 40.0 38.0 41.0 33.0 41.0 58-59 37.91997400625394 40.0 38.0 41.0 33.0 41.0 60-61 37.72021206778963 40.0 37.0 41.0 33.0 41.0 62-63 37.49473304937525 40.0 37.0 41.0 32.5 41.0 64-65 37.35542887536299 39.0 37.0 41.0 32.5 41.0 66-67 37.223045643473895 39.0 36.0 41.0 32.5 41.0 68-69 36.900296397335424 39.0 36.0 41.0 32.0 41.0 70-71 36.5495815693188 38.5 35.0 40.5 32.0 41.0 72-73 36.15985896462534 37.0 35.0 39.5 31.5 41.0 74-75 35.70294767364551 37.0 35.0 39.0 31.0 41.0 76-77 35.31438320615271 36.0 35.0 39.0 31.0 40.5 78-79 34.90684888025291 36.0 35.0 37.5 31.0 39.5 80-81 34.49749241849074 35.5 35.0 37.0 30.5 39.0 82-83 34.156058987788086 35.0 34.5 37.0 30.0 39.0 84-85 33.76521556364822 35.0 34.0 36.0 30.0 37.0 86-87 33.57422844470753 35.0 34.0 36.0 30.0 37.0 88-89 33.355391986548454 35.0 34.0 36.0 29.0 36.5 90-91 33.209350456606316 35.0 34.0 35.0 29.0 36.0 92-93 33.07286570326809 35.0 34.0 35.0 29.0 36.0 94-95 32.94721468003243 35.0 34.0 35.0 29.0 36.0 96-97 32.85724843758713 35.0 34.0 35.0 29.0 36.0 98-99 32.71327697065624 35.0 34.0 35.0 28.5 35.5 100-101 31.501383759484927 34.5 31.5 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1790977199694268 1101 2 0.1395323016638912 1101 3 0.2951305234528405 1101 4 0.1165747033595963 1101 5 0.10996496144969825 1101 6 0.023848460024758822 1101 7 0.18005348516909692 1101 8 0.03576054152595276 1101 9 0.14043580875473083 1101 10-11 0.13386021094857625 1101 12-13 0.2809040333060011 1101 14-15 0.34342389652868377 1101 16-17 0.23006223473498721 1101 18-19 0.3090618532122704 1101 20-21 0.16360442011822585 1101 22-23 0.1869822705385289 1101 24-25 0.29547210685745995 1101 26-27 0.1723142668619957 1101 28-29 0.2298289719127311 1101 30-31 0.32986935224465697 1101 32-33 0.19952847509664906 1101 34-35 0.1717588078866541 1101 36-37 0.11270060109294633 1101 38-39 0.26310415054448555 1101 40-41 0.3919703290097871 1101 42-43 0.3770462162869421 1101 44-45 0.4464568747563007 1101 46-47 0.44296071134402837 1101 48-49 0.2877759593097693 1101 50-51 0.23829011975243475 1101 52-53 0.25998549287493944 1101 54-55 0.42217079247377143 1101 56-57 0.4442368511816994 1101 58-59 0.44294012599870314 1101 60-61 0.274064127436624 1101 62-63 0.42991799979744627 1101 64-65 0.4907947925919345 1101 66-67 0.5117718552571731 1101 68-69 0.5050773834311073 1101 70-71 0.5820133622815291 1101 72-73 0.4644219323015619 1101 74-75 0.3707688044918598 1101 76-77 0.38963276810168423 1101 78-79 0.36519991167059374 1101 80-81 0.3525755229912946 1101 82-83 0.5241381409859471 1101 84-85 0.4511667452322925 1101 86-87 0.3744820400122606 1101 88-89 0.26671800690949965 1101 90-91 0.3300897257782367 1101 92-93 0.2318976705589293 1101 94-95 0.08134812048704987 1101 96-97 0.09266118705359361 1101 98-99 0.075465970689919 1101 100-101 0.15076397730834046 1102 1 0.2305877720572056 1102 2 0.20647341415821785 1102 3 0.31912730560546976 1102 4 0.22538940298972676 1102 5 0.25718035498434233 1102 6 0.19015184550579534 1102 7 0.25927612335451755 1102 8 0.3136796369333297 1102 9 0.31105699711115875 1102 10-11 0.324675526284139 1102 12-13 0.304424430233766 1102 14-15 0.30637651866981486 1102 16-17 0.29352539802276567 1102 18-19 0.31511217027809835 1102 20-21 0.288326903253747 1102 22-23 0.2472972465619634 1102 24-25 0.19119935833943202 1102 26-27 0.2824536320112685 1102 28-29 0.33950543443784653 1102 30-31 0.3799117100818705 1102 32-33 0.3121902101275964 1102 34-35 0.3148367283303273 1102 36-37 0.09321768130094199 1102 38-39 0.0961243879281497 1102 40-41 0.12496483709710304 1102 42-43 -0.0018232901150483372 1102 44-45 0.20038087333612964 1102 46-47 0.07813221002497528 1102 48-49 -0.018124026758208345 1102 50-51 -0.09067410191950387 1102 52-53 -0.0284590194818648 1102 54-55 0.055935606481298805 1102 56-57 0.08144478434395097 1102 58-59 0.030343332161152148 1102 60-61 0.03849388083985872 1102 62-63 0.2145491832551869 1102 64-65 0.0799462426456401 1102 66-67 0.12692730964329257 1102 68-69 0.23101206472489366 1102 70-71 0.12708232578413003 1102 72-73 0.058173196295378204 1102 74-75 0.12079239904308992 1102 76-77 0.10320615165021962 1102 78-79 0.023839000426548296 1102 80-81 0.02527921138142375 1102 82-83 0.007723074629076621 1102 84-85 0.02836362266196346 1102 86-87 -0.04895813544131755 1102 88-89 -0.2299169606648448 1102 90-91 -0.3193345671717083 1102 92-93 -0.14250460080987182 1102 94-95 -0.16793280747541672 1102 96-97 -0.13000171646940828 1102 98-99 -0.09538947785372898 1102 100-101 -0.23399911092629466 1103 1 0.19320953511663674 1103 2 0.12227915059676775 1103 3 0.2880661997079841 1103 4 0.15290836667698215 1103 5 0.18876662490594498 1103 6 0.18618903933086273 1103 7 0.16835185106424433 1103 8 0.14906745509502883 1103 9 0.24504881742023343 1103 10-11 0.23005021022267869 1103 12-13 0.18545623513421106 1103 14-15 0.2803462710251594 1103 16-17 0.2831619632082649 1103 18-19 0.2716698563599209 1103 20-21 0.18881979520068626 1103 22-23 0.21840246787670026 1103 24-25 0.24261697336120847 1103 26-27 0.21908766460708762 1103 28-29 0.1919861838876855 1103 30-31 0.14527168205126628 1103 32-33 0.2146476007406264 1103 34-35 0.2826471242421178 1103 36-37 0.14379519780580807 1103 38-39 0.16870889978992665 1103 40-41 0.1777430019121411 1103 42-43 0.17634367639166015 1103 44-45 0.166344077021094 1103 46-47 0.24531744969894476 1103 48-49 0.31364726973919943 1103 50-51 0.3600694877190094 1103 52-53 0.26771259010591564 1103 54-55 0.38060858276593024 1103 56-57 0.4038008303895495 1103 58-59 0.37987144267713546 1103 60-61 0.38019678102386223 1103 62-63 0.39944462172515216 1103 64-65 0.4207885541049947 1103 66-67 0.33039968269621056 1103 68-69 0.383213327741295 1103 70-71 0.4128746206464129 1103 72-73 0.39385812246916174 1103 74-75 0.3893596007773823 1103 76-77 0.32668874931679426 1103 78-79 0.3639974512029269 1103 80-81 0.33776358759802605 1103 82-83 0.3091586185826216 1103 84-85 0.29140256635077577 1103 86-87 0.28622375613269924 1103 88-89 0.25357510347770074 1103 90-91 0.11171690832814818 1103 92-93 0.07406921106542796 1103 94-95 0.14351732550603202 1103 96-97 0.31497592871395597 1103 98-99 0.17241395138743343 1103 100-101 0.2899406232747346 1104 1 0.3353483221933189 1104 2 0.2604558164403059 1104 3 0.33507153340386253 1104 4 0.32539197113445795 1104 5 0.2738347735761337 1104 6 0.21807745623036823 1104 7 0.27453189081494145 1104 8 0.27672008323737884 1104 9 0.23813452742514585 1104 10-11 0.24253588637753865 1104 12-13 0.19020125450550296 1104 14-15 0.3791967564404999 1104 16-17 0.39266319281615125 1104 18-19 0.40718707318247027 1104 20-21 0.251028058128675 1104 22-23 0.22742128795255923 1104 24-25 0.19442781259286335 1104 26-27 0.31588494994363003 1104 28-29 0.5046312142648688 1104 30-31 0.35291310083101735 1104 32-33 0.3038605314617655 1104 34-35 0.3291585800812058 1104 36-37 0.2568899899840389 1104 38-39 0.16257612063564153 1104 40-41 0.24392772147922415 1104 42-43 0.2377070752331747 1104 44-45 0.25817252568045035 1104 46-47 0.47339190872233416 1104 48-49 0.3684607753563611 1104 50-51 0.2860826078524852 1104 52-53 0.28752550843387326 1104 54-55 0.3021342584028659 1104 56-57 0.3476310103960074 1104 58-59 0.31449274287071205 1104 60-61 0.31917234516832593 1104 62-63 0.3884165326532596 1104 64-65 0.18051281488359905 1104 66-67 0.0647026443910832 1104 68-69 0.13169400592622793 1104 70-71 0.1257715842224627 1104 72-73 0.21092446950251542 1104 74-75 0.17690241046145871 1104 76-77 0.18672616935066344 1104 78-79 0.17384384039161915 1104 80-81 0.16922915626508228 1104 82-83 0.14250721218680695 1104 84-85 0.13360026732160435 1104 86-87 0.2001844993171389 1104 88-89 0.1780219649024133 1104 90-91 0.20069399849970182 1104 92-93 0.16842809009217774 1104 94-95 0.20950643614669673 1104 96-97 0.19482118031882578 1104 98-99 0.2556457807979484 1104 100-101 0.05515719426412247 1105 1 0.21106911508484671 1105 2 0.18173749262724215 1105 3 0.3267838664121143 1105 4 0.25084553852714464 1105 5 0.15579308562430327 1105 6 0.19738828147534093 1105 7 0.23796979233044624 1105 8 0.3071162581007627 1105 9 0.13265398593069477 1105 10-11 0.29457229560987486 1105 12-13 0.32165597903684073 1105 14-15 0.4572034786807819 1105 16-17 0.4417501562366297 1105 18-19 0.38427843682587337 1105 20-21 0.36967250308912725 1105 22-23 0.3629966258060833 1105 24-25 0.38488548221623375 1105 26-27 0.3374042459079689 1105 28-29 0.3450249316598786 1105 30-31 0.39187026348059106 1105 32-33 0.2153771571061256 1105 34-35 0.2666445512262712 1105 36-37 0.315440141689173 1105 38-39 0.1299701592430509 1105 40-41 0.3198770395801276 1105 42-43 0.26233448819622396 1105 44-45 0.2771458263507469 1105 46-47 0.34470570614954 1105 48-49 0.3561236068243474 1105 50-51 0.23257123383740463 1105 52-53 0.2553181600229095 1105 54-55 0.34561596864605093 1105 56-57 0.33778952678170526 1105 58-59 0.19120184440479449 1105 60-61 0.19509970085494643 1105 62-63 0.14374314374630615 1105 64-65 0.2023847672358059 1105 66-67 0.14718118508056932 1105 68-69 0.11193025184335426 1105 70-71 0.17899560858815988 1105 72-73 0.14395797234312369 1105 74-75 0.2163022076719372 1105 76-77 0.3231543648335631 1105 78-79 0.22881276405917106 1105 80-81 0.1734653867020839 1105 82-83 0.05718420022068216 1105 84-85 0.08635156765133445 1105 86-87 0.09112563230681303 1105 88-89 0.1911960271944011 1105 90-91 0.006596566650912905 1105 92-93 0.05661956751442432 1105 94-95 -0.043408663158245986 1105 96-97 0.08983503502246037 1105 98-99 0.1338593560854946 1105 100-101 -0.04821176268567484 1106 1 0.1457563689231094 1106 2 0.027012302014462364 1106 3 0.1156142544744796 1106 4 0.1484738398811274 1106 5 0.031376442490078205 1106 6 0.06659001148143062 1106 7 0.06744194725813912 1106 8 0.05762065742897704 1106 9 0.15304082213003056 1106 10-11 0.06989444236314313 1106 12-13 0.15296227111656435 1106 14-15 0.19302409830238076 1106 16-17 0.1088972253328464 1106 18-19 0.11685390165354193 1106 20-21 0.1996286212764815 1106 22-23 0.1290570534029527 1106 24-25 0.1552082234733163 1106 26-27 0.13874893921830989 1106 28-29 0.13888863051396783 1106 30-31 0.04354351303842918 1106 32-33 0.06395982286122148 1106 34-35 0.05000318193536657 1106 36-37 0.08033013990566218 1106 38-39 0.053555054387437906 1106 40-41 0.04309464283800679 1106 42-43 0.0802820938321176 1106 44-45 0.36341901199942583 1106 46-47 0.1645933449290311 1106 48-49 0.15520128989435733 1106 50-51 0.10140000559253792 1106 52-53 0.093892725663558 1106 54-55 0.19838895736534568 1106 56-57 0.2655015788602455 1106 58-59 0.2432260369943009 1106 60-61 0.11564937536410724 1106 62-63 0.19713376619179712 1106 64-65 0.19780662342532906 1106 66-67 0.1831314808462139 1106 68-69 0.20937082004036967 1106 70-71 0.2503643978811496 1106 72-73 0.15614297821384326 1106 74-75 0.2674824835217109 1106 76-77 0.15163815717338736 1106 78-79 0.28365295400313784 1106 80-81 0.2808806141741087 1106 82-83 0.29551938518567766 1106 84-85 0.2842632801195677 1106 86-87 0.21616418216338928 1106 88-89 0.2368293038021676 1106 90-91 0.15178926019905958 1106 92-93 0.22652271912776456 1106 94-95 0.19243564372462885 1106 96-97 0.27074254509187057 1106 98-99 0.19798263016662077 1106 100-101 0.06123128013346246 1107 1 0.15470453074107127 1107 2 0.11728098444438473 1107 3 0.23156713484607394 1107 4 0.10665980622665217 1107 5 0.1888138164697537 1107 6 0.08783768075197429 1107 7 0.1876167720846098 1107 8 0.14964203308240087 1107 9 0.2740517590277136 1107 10-11 0.18558292432542345 1107 12-13 0.17484641249997424 1107 14-15 0.2736636972138271 1107 16-17 0.1468851457742133 1107 18-19 0.15860011136822294 1107 20-21 0.11988989288722252 1107 22-23 0.20906516162083477 1107 24-25 0.3120382474837555 1107 26-27 0.32008754621421787 1107 28-29 0.3705855548324166 1107 30-31 0.3149684058804425 1107 32-33 0.18221871718539262 1107 34-35 0.15781757271927432 1107 36-37 0.1332358784240668 1107 38-39 0.08401949893236349 1107 40-41 0.06717482376316042 1107 42-43 0.17639666341067084 1107 44-45 0.392706692029833 1107 46-47 0.23330512690533567 1107 48-49 0.12916302799585822 1107 50-51 0.07822491023667055 1107 52-53 0.08339227695263673 1107 54-55 0.19417582619077933 1107 56-57 0.24482983829869198 1107 58-59 0.2643257964300645 1107 60-61 0.40535071245368925 1107 62-63 0.36574446367480107 1107 64-65 0.31663263317818036 1107 66-67 0.285477039604352 1107 68-69 0.30263870007859595 1107 70-71 0.4167269425127458 1107 72-73 0.31553958272517235 1107 74-75 0.34874629259080336 1107 76-77 0.31757498204736834 1107 78-79 0.21554852199606245 1107 80-81 0.2099344740460154 1107 82-83 0.3196719882838224 1107 84-85 0.24234693577627553 1107 86-87 0.269145791529688 1107 88-89 0.1849995518538563 1107 90-91 0.3599424641329989 1107 92-93 0.29064661392325064 1107 94-95 0.18297899395135886 1107 96-97 0.22356007416995283 1107 98-99 0.3526890368083002 1107 100-101 0.34457479344419895 1108 1 0.25596510058503696 1108 2 0.15282578998156993 1108 3 0.2774209383160269 1108 4 0.3651735696909171 1108 5 0.269052567310176 1108 6 0.18668926075359593 1108 7 0.2583060472674106 1108 8 0.1620336145905128 1108 9 0.30053460595991055 1108 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-0.5833387213389614 2316 74-75 -0.3829356814420706 2316 76-77 -0.6293322809575628 2316 78-79 -0.4124862781217118 2316 80-81 -0.27778599476779675 2316 82-83 -0.24879871560175104 2316 84-85 -0.3350830888231542 2316 86-87 -0.35837588731005354 2316 88-89 -0.3164009059424586 2316 90-91 -0.3394570343805867 2316 92-93 -0.21878451242663033 2316 94-95 -0.19452535131444648 2316 96-97 -0.15730532231774674 2316 98-99 -0.19990031420368837 2316 100-101 0.04478303377776527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 14.0 7 317.0 8 701.0 9 653.0 10 708.0 11 895.0 12 1085.0 13 1048.0 14 1114.0 15 1203.0 16 1454.0 17 1888.0 18 2507.0 19 3347.0 20 4049.0 21 5064.0 22 5707.0 23 5251.0 24 4758.0 25 4452.0 26 5001.0 27 5965.0 28 7255.0 29 8689.0 30 10803.0 31 13997.0 32 18105.0 33 25321.0 34 37378.0 35 57314.0 36 100018.0 37 235533.0 38 458561.0 39 135494.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.04229581331503 23.862474262182566 12.295555936856555 23.799673987645846 2 12.857604925328186 23.3500948465425 43.392872310214685 20.39942791791463 3 13.786542031700929 36.39576062674367 29.614959627467734 20.20273771408766 4 9.394334540088876 18.977918089322788 46.54024329564367 25.08750407494467 5 9.773613653872882 42.05614532376816 37.725567038257815 10.444673984101138 6 24.18288653797153 45.40454376598429 20.032052244472144 10.380517451572027 7 25.180380132361694 33.09454235351246 28.739423847411704 12.98565366671414 8 23.95513399061774 37.03727852684102 23.50978548460712 15.497801997934118 9 27.35084826323529 15.712903175989526 23.557128860168262 33.37911970060692 10-11 21.241299313897333 29.526823379825647 32.12302317580512 17.108854130471897 12-13 23.12672172763438 27.07587483710344 31.158433687909493 18.63896974735269 14-15 21.89303959542407 28.275619496167426 27.042246271441623 22.78909463696688 16-17 19.588303672152804 31.004748362522683 28.9004130689349 20.506534896389613 18-19 19.82816638606801 30.332536218673926 30.020224550416607 19.819072844841454 20-21 19.468820427383495 30.12158012990166 30.613108040056584 19.796491402658255 22-23 20.66402810085458 29.684337222265356 29.165102815275656 20.486531861604405 24-25 20.310077785393986 30.802055174697568 29.51246650990054 19.3754005300079 26-27 19.20066951895698 31.243946318374967 30.206080405760744 19.34930375690731 28-29 19.85749251687724 27.71191886227365 32.0105214121547 20.420067208694405 30-31 20.282584616229396 30.183942542729596 29.96296954081716 19.570503300223844 32-33 20.057117929479347 29.645689539432002 30.975838978568763 19.321353552519884 34-35 20.29931412541339 30.14343743699948 30.606563635349783 18.95068480223735 36-37 20.715471426181622 28.916155156069713 29.530010766386567 20.838362651362097 38-39 20.61283631104042 30.771570146406074 29.90163529393598 18.713958248617523 40-41 19.707763379701714 29.41080842266003 31.880428768128066 19.000999429510195 42-43 20.006845901166805 28.923331912108235 30.19196833384663 20.877853852878324 44-45 19.08425073402779 29.98415057596249 30.22096971022792 20.7106289797818 46-47 20.7978802806389 29.012007281924046 29.781722439272972 20.408389998164076 48-49 19.614858650817073 30.460508706066452 29.209504951854115 20.715127691262364 50-51 20.1755985152883 29.180984949127648 29.100640992919182 21.54277554266487 52-53 20.327667039844208 29.169229581397744 29.82653678372431 20.676566595033734 54-55 20.52429300013297 29.685587197007717 29.453487923202637 20.33663187965668 56-57 20.236088413051778 29.865698978694567 29.221774695131103 20.67643791312255 58-59 19.214954553838368 28.66187969957063 30.899014725500034 21.224151021090965 60-61 20.17925390227896 31.190522148301614 28.798711465129344 19.831512484290084 62-63 19.852957261950976 29.591021299320765 31.032537709727855 19.523483729000404 64-65 20.07385749938016 29.093197371705948 30.223022366579933 20.609922762333962 66-67 20.023832891792836 30.11347647938944 28.8682550685646 20.99443556025312 68-69 19.900022221097345 29.787037807609913 30.104439157520126 20.20850081377262 70-71 20.83419121936489 28.414815348610567 30.481848847344327 20.269144584680216 72-73 19.75666250595154 30.533858355530963 28.585657114179458 21.123822024338036 74-75 20.35155898135399 30.122419391506135 28.595136681636664 20.930884945503212 76-77 19.508735357070854 30.258178807804985 30.734130303303264 19.49895553182089 78-79 19.874706712477426 29.082497973259898 29.774892443369232 21.267902870893437 80-81 19.55584804930598 31.586708046714723 28.012916821470036 20.844527082509266 82-83 21.363400414412638 29.850837190745644 28.45350298797517 20.33225940686655 84-85 19.3770437226878 30.35473577363082 28.347929871406883 21.920290632274494 86-87 19.600446512841106 30.148883374689827 30.519161269730137 19.731508842738933 88-89 20.215421694810257 29.508147490972615 30.12819165297776 20.148239161239367 90-91 19.995332443294334 30.077641030569062 30.02397270852138 19.90305381761522 92-93 21.038400573123674 30.371822563312794 29.015029481979422 19.57474738158411 94-95 20.074811041622333 28.572016997672772 29.921627753712233 21.431544206992662 96-97 20.950307481440646 29.296213913562003 29.971064355533056 19.782414249464296 98-99 19.84156912206642 29.04759146884257 30.36746268208504 20.743376727005966 100-101 20.5180235738264 28.778258025075342 30.525295209086327 20.178423192011927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 70.0 1 58.5 2 137.5 3 197.0 4 134.0 5 129.0 6 247.0 7 487.5 8 694.5 9 690.5 10 541.0 11 425.5 12 404.5 13 406.0 14 406.5 15 395.5 16 383.5 17 396.0 18 387.0 19 399.0 20 443.0 21 488.0 22 532.5 23 629.5 24 726.0 25 810.5 26 1155.5 27 1641.5 28 1912.0 29 2588.5 30 4830.5 31 7596.0 32 14672.0 33 26157.0 34 38240.5 35 51817.0 36 75787.0 37 97902.0 38 103011.0 39 106856.0 40 96493.5 41 78153.0 42 65628.0 43 59556.0 44 58016.5 45 55779.0 46 47182.5 47 31767.5 48 24060.0 49 18903.0 50 14832.0 51 9337.5 52 4861.0 53 3908.5 54 3562.0 55 3663.0 56 3772.5 57 3794.0 58 4288.0 59 4457.5 60 5508.5 61 6347.0 62 4769.5 63 2761.5 64 1563.5 65 1258.0 66 1080.5 67 876.0 68 589.0 69 414.0 70 387.0 71 371.5 72 281.0 73 299.5 74 309.0 75 356.0 76 353.0 77 224.5 78 212.0 79 285.5 80 259.5 81 162.0 82 92.0 83 60.0 84 32.5 85 12.5 86 6.5 87 6.5 88 8.0 89 4.5 90 1.5 91 2.5 92 1.5 93 0.0 94 0.0 95 0.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0055762161512956125 2 0.008407218197338 3 0.0018015467565724286 4 4.2893970394581633E-4 5 0.0040320332170906735 6 0.005833579973663102 7 0.006090943796030592 8 0.005233064388138959 9 0.00917930966444047 10-11 0.008235642315759673 12-13 0.004117821157879837 14-15 0.0 16-17 0.0 18-19 2.1446985197290817E-4 20-21 0.0012010311710482858 22-23 5.576216151295612E-4 24-25 0.001887334697361592 26-27 0.00446097292103649 28-29 0.0036030935131448573 30-31 0.011838735828904531 32-33 5.576216151295612E-4 34-35 0.0 36-37 0.0 38-39 2.573638223674898E-4 40-41 0.0 42-43 3.4315176315665303E-4 44-45 6.434095559187244E-4 46-47 0.003345729690777367 48-49 0.0035173055723556937 50-51 0.004418078950641909 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0015012889638103572 64-65 0.0036030935131448573 66-67 0.004203609098669 68-69 0.009093521723651306 70-71 7.720914671024694E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.01784389168414596 82-83 0.013726070526266123 84-85 0.017672315802567635 86-87 0.016213920809151856 88-89 0.01574208713481146 90-91 0.014841313756525245 92-93 0.010509022746672501 94-95 0.028224232519634716 96-97 0.012653721266401581 98-99 0.014798419786130664 100-101 0.015227359490076479 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1165665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 11.647437701328032 #Duplication Level Percentage of deduplicated Percentage of total 1 73.76240647404353 8.591430341064575 2 9.76083661905849 2.273774728666502 3 3.6062488683121363 1.26010677087451 4 1.9890754840267046 0.9267053113375978 5 1.3486183696380554 0.7853974221612915 6 0.8998325500947693 0.6288446141313577 7 0.6786105999686166 0.5532852280616711 8 0.5516875517256622 0.514059711145827 9 0.4639819655742121 0.48637809347088273 >10 4.378853492829445 11.15266957024874 >50 1.0008151512099752 8.262783454969034 >100 1.2747689674352223 31.484009832309596 >500 0.20546536216893532 16.48379409209762 >1k 0.07732567393454555 15.684277798276733 >5k 0.0014728699797056295 0.9124830311840607 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 5527 0.47414994874170535 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 5111 0.4384621653734134 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 4134 0.35464734722240093 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 3969 0.340492336992189 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 3637 0.3120107406501868 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3612 0.3098660421304577 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3389 0.2907353313344743 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 3370 0.2891053604594802 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3257 0.27941132315030476 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 3190 0.27366353111743086 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 3162 0.27126146877533425 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 3147 0.2699746496634968 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 3067 0.26311161440036374 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 2858 0.24518193477542863 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2739 0.23497316982151817 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 2593 0.22244813046630035 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 2585 0.22176182693998706 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2582 0.22150446311761957 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2580 0.22133288723604122 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2563 0.21987449224262545 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2507 0.2150703675584323 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 2454 0.21052360669660666 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 2454 0.21052360669660666 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 2453 0.2104378187558175 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 2452 0.21035203081502832 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 2403 0.20614842171635933 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2308 0.19799856734138882 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 2269 0.19465283765061145 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2251 0.1931086547164065 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2221 0.19053501649273163 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2120 0.18187043447302612 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 2064 0.17706630978883298 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 2028 0.1739779439204231 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 2015 0.172862700690164 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1962 0.16831593982833834 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1958 0.16797278806518168 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1953 0.16754384836123584 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1930 0.1655707257230851 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1896 0.16265393573625356 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 1804 0.15476144518365054 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1723 0.14781262197972833 No Hit CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG 1693 0.1452389837560534 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 1681 0.14420952846658344 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 1637 0.14043485907186026 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1577 0.1352875826245105 No Hit CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT 1556 0.13348603586793806 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 1521 0.13048345794031735 No Hit CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC 1509 0.12945400265084736 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1497 0.1284245473613774 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 1456 0.12490724178902171 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1426 0.12233360356534682 No Hit CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA 1418 0.12164730003903351 No Hit GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC 1410 0.1209609965127202 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1403 0.12036048092719606 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 1390 0.11924523769693694 No Hit ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT 1351 0.11589950800615957 No Hit GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA 1345 0.11538478036142458 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1326 0.11375480948643049 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 1320 0.11324008184169551 No Hit GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA 1314 0.11272535419696054 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1311 0.11246799037459304 No Hit ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA 1296 0.1111811712627556 No Hit CATTTATAGAAACCTGAAACATTGGAGTACTTCTACTGTTCGCAGTCATA 1294 0.11100959538117726 No Hit ATATGGTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGAT 1291 0.11075223155880978 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1276 0.10946541244697232 No Hit CACTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCG 1273 0.10920804862460484 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1264 0.10843595715750237 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 1263 0.10835016921671321 No Hit TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC 1262 0.10826438127592404 No Hit CTTCTGAGCGTTCTATCATTCTAGCCTCGTACCAACACATGATCTAGGAG 1239 0.10629125863777329 No Hit CTATCATTCTAGCCTCGTACCAACACATGATCTAGGAGGCTGCTGACCTC 1236 0.10603389481540579 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1217 0.1044039239404117 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 1216 0.10431813599962253 No Hit GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT 1212 0.1039749842364659 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1195 0.1025165892430501 No Hit ATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGCCA 1195 0.1025165892430501 No Hit TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA 1194 0.10243080130226095 No Hit ATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAG 1176 0.10088661836805601 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.578794078916327E-5 0.0 3 0.0 0.0 0.0 8.578794078916327E-5 0.0 4 0.0 0.0 0.0 8.578794078916327E-5 0.0 5 0.0 0.0 0.0 8.578794078916327E-5 0.0 6 0.0 0.0 0.0 8.578794078916327E-5 0.0 7 0.0 0.0 0.0 8.578794078916327E-5 0.0 8 0.0 0.0 0.0 8.578794078916327E-5 0.0 9 0.0 0.0 0.0 8.578794078916327E-5 0.0 10-11 0.0 0.0 0.0 8.578794078916327E-5 0.0 12-13 0.0 0.0 0.0 8.578794078916327E-5 0.0 14-15 0.0 0.0 0.0 1.286819111837449E-4 0.0 16-17 0.0 0.0 0.0 5.147276447349796E-4 0.0 18-19 0.0 0.0 0.0 9.43667348680796E-4 0.0 20-21 0.0 0.0 0.0 0.0020589105789399183 0.0 22-23 0.0 0.0 0.0 0.005447534240111867 0.0 24-25 0.0 0.0 0.0 0.009736931279570031 0.0 26-27 0.0 0.0 0.0 0.011452690095353296 0.0 28-29 0.0 0.0 0.0 0.012525039355217836 0.0 30-31 0.0 0.0 0.0 0.015141571549287317 0.0 32-33 0.0 0.0 0.0 0.019388074618350896 0.0 34-35 0.0 0.0 0.0 0.024578245036095278 0.0 36-37 0.0 0.0 0.0 0.03191311397356873 0.0 38-39 0.0 0.0 0.0 0.047097579493250635 0.0 40-41 0.0 0.0 0.0 0.06661433602278527 0.0 42-43 0.0 0.0 0.0 0.08608819858192535 0.0 44-45 0.0 0.0 0.0 0.10684888025290284 4.2893970394581636E-5 46-47 0.0 0.0 0.0 0.13086950367386857 8.578794078916327E-5 48-49 0.0 0.0 0.0 0.15746376531850917 8.578794078916327E-5 50-51 0.0 0.0 0.0 0.1881758481210296 8.578794078916327E-5 52-53 0.0 0.0 0.0 0.22313443399261368 8.578794078916327E-5 54-55 0.0 0.0 0.0 0.25830748971617057 8.578794078916327E-5 56-57 0.0 0.0 0.0 0.29498183440353787 8.578794078916327E-5 58-59 0.0 0.0 0.0 0.33731818318298995 8.578794078916327E-5 60-61 0.0 0.0 0.0 0.3879759622189909 8.578794078916327E-5 62-63 0.0 0.0 0.0 0.4392771508109105 8.578794078916327E-5 64-65 0.0 0.0 0.0 0.4949964183534721 8.578794078916327E-5 66-67 0.0 0.0 0.0 0.5598092076196849 8.578794078916327E-5 68-69 0.0 0.0 0.0 0.6349165497805973 8.578794078916327E-5 70-71 0.0 0.0 0.0 0.7232781287934356 8.578794078916327E-5 72-73 0.0 0.0 0.0 0.819918244092428 8.578794078916327E-5 74-75 0.0 0.0 0.0 0.9127407960263025 8.578794078916327E-5 76-77 0.0 0.0 0.0 1.0136703083647531 8.578794078916327E-5 78-79 0.0 0.0 0.0 1.1375909888346996 8.578794078916327E-5 80-81 0.0 0.0 0.0 1.2799560765743159 8.578794078916327E-5 82-83 0.0 0.0 0.0 1.4318007317711348 8.578794078916327E-5 84-85 0.0 0.0 0.0 1.593897045892259 8.578794078916327E-5 86-87 0.0 0.0 0.0 1.7696765365692544 8.578794078916327E-5 88-89 0.0 0.0 0.0 1.9586244761573866 8.578794078916327E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACATAT 425 0.0 72.64158 1 ACATATA 525 0.0 58.805088 2 TGCATAG 25 0.005022793 56.9957 2 AAAGACA 75 2.386514E-9 50.667194 4 CATATAG 640 0.0 48.982784 3 GATTATC 50 2.8268465E-5 47.500492 9 GGCTCGG 95 3.8562575E-10 45.00047 9 ATGGGCG 130 0.0 43.846607 5 GGTATCA 1485 0.0 43.818245 1 GGGAGAC 55 4.9630515E-5 43.182266 7 GGGATAC 55 4.9630515E-5 43.182266 7 CATTCCG 125 3.6379788E-12 41.800434 9 TGGGTAT 415 0.0 41.205246 6 GCGGCTC 105 1.027729E-9 40.71471 7 TACCTGG 105 1.027729E-9 40.711216 2 GTATCAA 3280 0.0 40.400917 1 ATATGGA 190 0.0 40.000416 4 GGATGAC 995 0.0 39.623528 8 GGGCCTA 85 3.6404708E-7 39.118053 7 GTACATG 6140 0.0 38.90993 1 >>END_MODULE