FastQCFastQC Report
Fri 10 Feb 2017
SRR2568678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165665
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG54150.4645416993733191No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC50040.42928285570897295No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT41570.35662046986055174No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT41180.35327474016977434No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC38250.3281388735185495No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG35280.302659855104168No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT33390.28644593429501614No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA32660.28018341461740726No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA32070.2751219261108466No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT31970.27426404670295496No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA31940.2740066828805875No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG31480.270060437604286No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT30850.2646557973345687No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA30340.2602806123543214No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT30130.2584790655977489No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC27990.240120446268868No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT27350.23463001805836153No Hit
CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC26890.23068377278206No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG26640.22853907426233097No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG25910.222276554584722No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT25080.21515615549922146No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC24930.21386933638738403No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC24920.21378354844659486No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA24620.21120991022291996No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC24330.2087220599400342No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC24050.20631999759793768No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG23150.19859908292691295No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG23060.1978269914598105No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22520.19319444265719568No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT22430.1924223511900932No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG22240.1907923803150991No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG22110.18967713708483996No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG21090.18092676712434533No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT20380.17483582332831474No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC20110.17251954892700733No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT19590.16805857600597085No Hit
GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG19540.16762963630202501No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT19400.16642860513097674No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA18860.16179605632836191No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT18790.16119554074283776No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG18630.15982293369021117No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA16820.14429531640737261No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG16770.1438663767034268No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA16390.14060643495343858No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC16310.1399201314271253No Hit
CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG16240.13931961584160113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16060.1377754329073962No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT15940.13674597761792626No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG15580.13365761174951638No Hit
TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA15160.1300545182363715No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC15100.12953979059163653No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT15100.12953979059163653No Hit
CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT14960.12833875942058823No Hit
CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC14840.1273093041311183No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG14720.12627984884164833No Hit
CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA14000.12010311710482857No Hit
ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT13680.11735790299957534No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG13660.11718632711799702No Hit
ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT13650.11710053917720786No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC13640.11701475123641869No Hit
GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA13300.11409796124958714No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG13180.11306850596011718No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC13110.11246799037459304No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC13080.11221062655222555No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA13010.11161011096670141No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC12990.11143853508512308No Hit
ATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAG12790.10972277626933981No Hit
TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC12780.10963698832855066No Hit
CATTTATAGAAACCTGAAACATTGGAGTACTTCTACTGTTCGCAGTCATA12770.10955120038776149No Hit
GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA12650.10852174509829154No Hit
ATATGGTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGAT12520.10740650186803241No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA12330.1057765309930383No Hit
CACTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCG12190.10457549982199002No Hit
CTTCTGAGCGTTCTATCATTCTAGCCTCGTACCAACACATGATCTAGGAG12140.10414656011804421No Hit
GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT12060.1034602565917309No Hit
ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA12040.10328868071015258No Hit
ATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGCCA11850.10165870983515847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.10122977013121265No Hit
GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG11780.10105819424963433No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC11750.10080083042726684No Hit
CTATCATTCTAGCCTCGTACCAACACATGATCTAGGAGGCTGCTGACCTC11740.10071504248647767No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC11710.10045767866411019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG200.002074518771.246249
GACATAT4200.070.235851
TACCTGG850.067.144572
ACAACGC250.005018082457.0092163
TAAGATC250.005018082457.0092163
GCATGAC250.005022344656.9969869
CCGCACA250.005022344656.9969869
ACATATA5400.054.606772
AGATCCT352.7857348E-454.294495
TAACCGC405.383918E-447.5076836
GGTATCA13700.045.148081
CATATAG6600.044.6284263
ATGGGTA6250.043.3270035
CGGGAGT3400.043.3158264
AACGCGT554.958622E-543.18886
CGCGTAC554.9649032E-543.179548
GTCGGGA3500.042.125222
TCGGGAG3500.042.0782283
GGATGCC1700.041.909558
CATGGGT7950.040.63554
Produced by FastQC (version 0.11.5)