##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568678_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1165665 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.75787897895193 34.0 31.0 34.0 31.0 34.0 2 32.86949852659212 34.0 31.0 34.0 31.0 34.0 3 32.93091754492071 34.0 31.0 34.0 31.0 34.0 4 36.3223722081387 37.0 37.0 37.0 35.0 37.0 5 36.37431337476891 37.0 37.0 37.0 35.0 37.0 6 36.400873321237235 37.0 37.0 37.0 35.0 37.0 7 36.33220007463551 37.0 37.0 37.0 35.0 37.0 8 36.33775312804279 37.0 37.0 37.0 35.0 37.0 9 38.17199195309115 39.0 39.0 39.0 37.0 39.0 10-11 38.08451656350667 39.0 39.0 39.0 36.0 39.0 12-13 38.105418366340245 39.0 39.0 39.0 37.0 39.0 14-15 39.55779147525232 41.0 39.5 41.0 37.0 41.0 16-17 39.54906941531229 41.0 40.0 41.0 37.0 41.0 18-19 39.657758017955416 41.0 40.0 41.0 37.0 41.0 20-21 39.635634594845 41.0 40.0 41.0 37.0 41.0 22-23 39.60040534802023 41.0 40.0 41.0 37.0 41.0 24-25 39.5601587934784 41.0 40.0 41.0 37.0 41.0 26-27 39.45667108474562 41.0 39.0 41.0 36.5 41.0 28-29 39.37044648333784 41.0 39.0 41.0 36.0 41.0 30-31 39.33849991206736 41.0 39.0 41.0 36.0 41.0 32-33 39.17778435485324 41.0 39.0 41.0 35.5 41.0 34-35 39.10163726284996 41.0 39.0 41.0 35.0 41.0 36-37 39.00871433902536 41.0 39.0 41.0 35.0 41.0 38-39 38.87916725645876 40.0 38.5 41.0 35.0 41.0 40-41 38.83047187656831 40.0 38.0 41.0 35.0 41.0 42-43 38.72149030810739 40.0 38.0 41.0 35.0 41.0 44-45 38.63218120128853 40.0 38.0 41.0 35.0 41.0 46-47 38.46402439809036 40.0 38.0 41.0 34.5 41.0 48-49 38.32048658920016 40.0 38.0 41.0 34.0 41.0 50-51 38.32630429840477 40.0 38.0 41.0 34.5 41.0 52-53 38.46121612984862 40.0 38.0 41.0 35.0 41.0 54-55 38.36657616038914 40.0 38.0 41.0 34.0 41.0 56-57 38.26471241737549 40.0 38.0 41.0 34.0 41.0 58-59 38.10740135459159 40.0 38.0 41.0 34.0 41.0 60-61 38.076814951122316 40.0 38.0 41.0 34.0 41.0 62-63 37.905698463966914 40.0 37.0 41.0 34.0 41.0 64-65 37.66707115680749 39.5 37.0 41.0 33.0 41.0 66-67 37.42630815886211 39.0 36.5 41.0 33.0 41.0 68-69 37.075497248351795 39.0 36.0 41.0 33.0 41.0 70-71 36.703902922366204 38.0 35.5 40.0 33.0 41.0 72-73 36.28887630665757 37.0 35.0 39.5 32.0 41.0 74-75 35.83359155503511 37.0 35.0 39.0 32.0 41.0 76-77 34.03367648509649 35.5 33.0 37.5 29.0 39.0 78-79 34.913241797600506 36.0 35.0 37.0 31.0 39.0 80-81 34.74501765086882 35.5 35.0 37.0 31.0 39.0 82-83 34.471970077166254 35.0 35.0 37.0 31.0 39.0 84-85 34.08560006519883 35.0 35.0 36.0 31.0 37.0 86-87 33.89679753617034 35.0 34.5 36.0 31.0 37.0 88-89 33.69498698167999 35.0 34.0 36.0 31.0 37.0 90-91 33.58520586961091 35.0 34.0 35.0 31.0 36.0 92-93 33.47775046861663 35.0 34.0 35.0 31.0 36.0 94-95 33.37442232545371 35.0 34.0 35.0 31.0 36.0 96-97 33.292202734061675 35.0 34.0 35.0 30.0 36.0 98-99 33.20677467368412 35.0 34.0 35.0 30.0 36.0 100-101 32.175340685359856 34.5 32.5 35.0 27.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32811812299456733 1101 2 0.26263986795330396 1101 3 0.20613726547176725 1101 4 0.2558064173402528 1101 5 0.12838311963988502 1101 6 0.1736443128029279 1101 7 0.20290931968360582 1101 8 0.18331453418266364 1101 9 0.2159093046043168 1101 10-11 0.16808258005463017 1101 12-13 0.2674260144407299 1101 14-15 0.34284514703938385 1101 16-17 0.33526033004037004 1101 18-19 0.34984300646232214 1101 20-21 0.3429381917826291 1101 22-23 0.338420270938272 1101 24-25 0.2883130391470061 1101 26-27 0.31366316169782493 1101 28-29 0.3214393694929072 1101 30-31 0.31147954533074085 1101 32-33 0.295146993006405 1101 34-35 0.38223038000653276 1101 36-37 0.41894374319066685 1101 38-39 0.3488942577815166 1101 40-41 0.31510582935796094 1101 42-43 0.37017448883923976 1101 44-45 0.30731186831254576 1101 46-47 0.2600957617891382 1101 48-49 0.3276101623842109 1101 50-51 0.272327666343692 1101 52-53 0.2708019793355092 1101 54-55 0.2509594860973152 1101 56-57 0.3234504913248415 1101 58-59 0.3476358994258817 1101 60-61 0.31549799175040505 1101 62-63 0.3412607732341755 1101 64-65 0.24585109328371146 1101 66-67 0.33533337522864315 1101 68-69 0.2441346117901162 1101 70-71 0.30976695054423686 1101 72-73 0.2818743227384388 1101 74-75 0.2635673177547133 1101 76-77 0.10209314563102367 1101 78-79 0.2911278304131599 1101 80-81 0.3013988720267733 1101 82-83 0.2905443105173333 1101 84-85 0.24800739434584074 1101 86-87 0.2655156400376839 1101 88-89 0.300506039127562 1101 90-91 0.2283439631834554 1101 92-93 0.23034656565707934 1101 94-95 0.13358342698881387 1101 96-97 0.18032420265671334 1101 98-99 0.2026565651466683 1101 100-101 0.10286213898224617 1102 1 0.39551202616252823 1102 2 0.13745953816889056 1102 3 0.16885113322543077 1102 4 0.1928651430547177 1102 5 0.23237104821805588 1102 6 0.21100909355336483 1102 7 0.14369598449266618 1102 8 0.15788297860513723 1102 9 0.26384781495670495 1102 10-11 0.23354116824656046 1102 12-13 0.24263432062160462 1102 14-15 0.2446558603139124 1102 16-17 0.3185593201935788 1102 18-19 0.34410335098771583 1102 20-21 0.33760245255410126 1102 22-23 0.2655389492838651 1102 24-25 0.30203832666346386 1102 26-27 0.18961199972235931 1102 28-29 0.23786072773010147 1102 30-31 0.23231701707345565 1102 32-33 0.23397868071342032 1102 34-35 0.3206688251930174 1102 36-37 0.20740791767349265 1102 38-39 0.36779068444290886 1102 40-41 0.3813160725391356 1102 42-43 0.31619408804646554 1102 44-45 0.34421037578864855 1102 46-47 0.2284004146346419 1102 48-49 0.30101573249597635 1102 50-51 0.2744640967802141 1102 52-53 0.5140707909341415 1102 54-55 0.39242061800658945 1102 56-57 0.3635140393161578 1102 58-59 0.3492538115103372 1102 60-61 0.5051234675738527 1102 62-63 0.49970661138004857 1102 64-65 0.43277724009124796 1102 66-67 0.5200702847469074 1102 68-69 0.4397665862271012 1102 70-71 0.4188372578127044 1102 72-73 0.3438133903716647 1102 74-75 0.32736305185984804 1102 76-77 0.24146559798126788 1102 78-79 0.30342901823027546 1102 80-81 0.361604301919499 1102 82-83 0.36998379386407265 1102 84-85 0.27013964517614397 1102 86-87 0.2234729586180535 1102 88-89 0.3272740574998494 1102 90-91 0.27209893489246895 1102 92-93 0.2352272671118456 1102 94-95 0.26511181460459454 1102 96-97 0.1513090841671243 1102 98-99 0.17634470809819192 1102 100-101 0.18582563708794453 1103 1 0.20680848258599838 1103 2 0.13157167697775662 1103 3 0.030109887666689872 1103 4 0.08184100149196638 1103 5 0.12258963775411758 1103 6 0.07765472360676284 1103 7 0.16805269442099302 1103 8 0.11130156486276377 1103 9 0.11971457123387808 1103 10-11 0.20577335098643346 1103 12-13 0.1670116232087011 1103 14-15 0.3426215924905023 1103 16-17 0.2262709123237059 1103 18-19 0.1298100992327278 1103 20-21 0.19948718284149436 1103 22-23 0.2325159059962516 1103 24-25 0.32860388098505666 1103 26-27 0.25153340748159536 1103 28-29 0.18104343071634332 1103 30-31 0.20575402402783993 1103 32-33 0.1513498690225319 1103 34-35 0.1430822438202597 1103 36-37 0.170342668656005 1103 38-39 0.2621498327059584 1103 40-41 0.21047230627503666 1103 42-43 0.2794997117888798 1103 44-45 0.17693958949741528 1103 46-47 0.3599168129478869 1103 48-49 0.3368879391684132 1103 50-51 0.4157681550707579 1103 52-53 0.4529116189024549 1103 54-55 0.4650169117378127 1103 56-57 0.3656470699408203 1103 58-59 0.4505430553806917 1103 60-61 0.4795675682381457 1103 62-63 0.31854500101363925 1103 64-65 0.33373064945691766 1103 66-67 0.4125033534833591 1103 68-69 0.41953814090112473 1103 70-71 0.3714480176305912 1103 72-73 0.23914121320513715 1103 74-75 0.3029986581036255 1103 76-77 0.2668965070498288 1103 78-79 0.32123142296106266 1103 80-81 0.37603453071316295 1103 82-83 0.2557734249993544 1103 84-85 0.20840930860398998 1103 86-87 0.2586654026490507 1103 88-89 0.3077781371002075 1103 90-91 0.26114230856418885 1103 92-93 0.1587475667606455 1103 94-95 0.23756785286391136 1103 96-97 0.2526469922808374 1103 98-99 0.23257342389491953 1103 100-101 0.277423524957328 1104 1 0.2938539924131547 1104 2 0.19189136577921317 1104 3 0.14779432191295427 1104 4 0.20606145598907943 1104 5 0.12029227334162584 1104 6 0.16965011038064404 1104 7 0.14361498915878457 1104 8 0.16227549384608864 1104 9 0.10429984793958624 1104 10-11 0.2756760274529668 1104 12-13 0.25110080238185617 1104 14-15 0.3359810625059225 1104 16-17 0.33412079565199093 1104 18-19 0.19623204664676308 1104 20-21 0.24129801282450813 1104 22-23 0.2223733750664607 1104 24-25 0.2386071272608561 1104 26-27 0.3844887485301527 1104 28-29 0.2956281802490537 1104 30-31 0.2826047480583185 1104 32-33 0.17185921062046106 1104 34-35 0.1145376759680019 1104 36-37 0.25164569231681355 1104 38-39 0.20571457648600244 1104 40-41 0.12741990065518394 1104 42-43 0.08398095969059227 1104 44-45 0.1343070170504319 1104 46-47 0.18697031360777316 1104 48-49 0.16351923900128185 1104 50-51 0.22480852449879052 1104 52-53 0.203637406633014 1104 54-55 0.3043614044910399 1104 56-57 0.3597487440387823 1104 58-59 0.36144064601514003 1104 60-61 0.4962757150955994 1104 62-63 0.343950114961622 1104 64-65 0.3058427356839175 1104 66-67 0.34056042504663253 1104 68-69 0.24359045495934595 1104 70-71 0.15782041078325904 1104 72-73 0.06810132285711035 1104 74-75 0.005212507215610174 1104 76-77 0.044500256082180556 1104 78-79 0.13531184366360094 1104 80-81 0.13188869431620986 1104 82-83 0.0174130809866071 1104 84-85 0.06822568592667011 1104 86-87 -0.0032729847010202207 1104 88-89 4.889993610035503E-4 1104 90-91 0.011203580863842433 1104 92-93 -0.05245828134508912 1104 94-95 0.04056607989789285 1104 96-97 -0.006909867950803061 1104 98-99 0.12081598636260082 1104 100-101 0.09580179285184443 1105 1 0.294892127678402 1105 2 0.13312110617766848 1105 3 0.16170901099482649 1105 4 0.06901405587452558 1105 5 0.21910281994450997 1105 6 0.14239156148669707 1105 7 0.19748482460515504 1105 8 0.1169593898816288 1105 9 0.2198976126108647 1105 10-11 0.24619850071106697 1105 12-13 0.22976049525635744 1105 14-15 0.39779993741110786 1105 16-17 0.45601945120393594 1105 18-19 0.3907479253755284 1105 20-21 0.4541249335198714 1105 22-23 0.43799590792300336 1105 24-25 0.4546229518092275 1105 26-27 0.41461885890337413 1105 28-29 0.2892445674996367 1105 30-31 0.34608725165745113 1105 32-33 0.38514859977508564 1105 34-35 0.495616523937791 1105 36-37 0.5127850868894939 1105 38-39 0.5443009903623874 1105 40-41 0.5782560204093627 1105 42-43 0.4923164215398472 1105 44-45 0.5262616138708225 1105 46-47 0.5633320800437929 1105 48-49 0.3550039175566866 1105 50-51 0.45365591377726133 1105 52-53 0.3125201873262142 1105 54-55 0.3278307112914476 1105 56-57 0.44798056651281115 1105 58-59 0.4370663329452782 1105 60-61 0.39234995773093573 1105 62-63 0.32758618130939254 1105 64-65 0.36097661933536784 1105 66-67 0.33368918864978525 1105 68-69 0.26825705196549166 1105 70-71 0.3307589431134872 1105 72-73 0.37427672804914636 1105 74-75 0.33926373091876627 1105 76-77 0.37733096940592503 1105 78-79 0.30892279352350727 1105 80-81 0.19597008489501633 1105 82-83 0.11790031763113973 1105 84-85 0.24215204314582195 1105 86-87 0.288315797633075 1105 88-89 0.3219839141429688 1105 90-91 0.23499719609008451 1105 92-93 0.19520180240233032 1105 94-95 0.3019584776130273 1105 96-97 0.34545820276236583 1105 98-99 0.32460590571119496 1105 100-101 0.3045666344128648 1106 1 0.22061909437132954 1106 2 0.13830072064195065 1106 3 0.13636271252418908 1106 4 0.14031921468135522 1106 5 0.14154515307472337 1106 6 0.1490555776572009 1106 7 0.09275276004204613 1106 8 0.10129235660428293 1106 9 0.20244149442351045 1106 10-11 0.20253358906919772 1106 12-13 0.21291838803759333 1106 14-15 0.21804149174131737 1106 16-17 0.2864051912868675 1106 18-19 0.2585725430352639 1106 20-21 0.22093034870647443 1106 22-23 0.23171051636793294 1106 24-25 0.2843194390348174 1106 26-27 0.14855053555275077 1106 28-29 0.24983453822032686 1106 30-31 0.14323465516204692 1106 32-33 0.04794743083196096 1106 34-35 0.28091797452709244 1106 36-37 0.31144568837872555 1106 38-39 0.3021191799496492 1106 40-41 0.22927020675658127 1106 42-43 0.2752658664219112 1106 44-45 0.19263536355498445 1106 46-47 0.22639750890397892 1106 48-49 0.34998639827247047 1106 50-51 0.1955660173695506 1106 52-53 0.2485055959732705 1106 54-55 0.1818656272434751 1106 56-57 0.10556216216104275 1106 58-59 0.11639515671949141 1106 60-61 0.21390220718473074 1106 62-63 0.2962405033521165 1106 64-65 0.2933448438497308 1106 66-67 0.16739718323979957 1106 68-69 0.11346033511279074 1106 70-71 0.12632719586677155 1106 72-73 0.29497549296747394 1106 74-75 0.1434063077955372 1106 76-77 0.1151227407300297 1106 78-79 0.25089968590345535 1106 80-81 0.15333719471473017 1106 82-83 0.18408531235491665 1106 84-85 0.11907721369410496 1106 86-87 0.17062308550668348 1106 88-89 0.07049765461233193 1106 90-91 0.08501549566054933 1106 92-93 0.12969537804551123 1106 94-95 0.1334918887272778 1106 96-97 0.29765823843259653 1106 98-99 0.3081139983943473 1106 100-101 0.35103453863830225 1107 1 0.1252440739666696 1107 2 0.025870853277986328 1107 3 0.05328260133891405 1107 4 0.07327697454476123 1107 5 0.04219342731948217 1107 6 0.001188659722636487 1107 7 0.06745021545642516 1107 8 0.08502993850073182 1107 9 0.11396382382714165 1107 10-11 0.09170927146616492 1107 12-13 0.020287669847206757 1107 14-15 0.07138352595370634 1107 16-17 0.04863400090392389 1107 18-19 0.011694168602666366 1107 20-21 0.08968566062379324 1107 22-23 0.0495281335788178 1107 24-25 0.07308350292267107 1107 26-27 0.0620853583560006 1107 28-29 0.1058316689228036 1107 30-31 0.09402551295478645 1107 32-33 0.17267259060140816 1107 34-35 0.025783472373966276 1107 36-37 0.16044775681729107 1107 38-39 0.13124002075191754 1107 40-41 0.0957260188850313 1107 42-43 0.016479600731393873 1107 44-45 0.08135390493410455 1107 46-47 0.02794734340589855 1107 48-49 0.1410702708125129 1107 50-51 0.18869932244930254 1107 52-53 0.1698003204969467 1107 54-55 0.24288456506693024 1107 56-57 0.2052185208661541 1107 58-59 0.29259868678312273 1107 60-61 0.2921185371968633 1107 62-63 0.3463834700861952 1107 64-65 0.14190356597007536 1107 66-67 0.22325690340665005 1107 68-69 0.10587899811518042 1107 70-71 0.19102970167396194 1107 72-73 0.08984945922717458 1107 74-75 0.11185668021411033 1107 76-77 0.003563855372412661 1107 78-79 0.13775517646213586 1107 80-81 0.11785569455647504 1107 82-83 0.191471607627399 1107 84-85 0.22797103972301613 1107 86-87 0.37846284321044976 1107 88-89 0.3197701758006062 1107 90-91 0.36111142706717914 1107 92-93 0.23044190120642583 1107 94-95 0.3371089356039718 1107 96-97 0.3316820986647002 1107 98-99 0.37681236885713076 1107 100-101 0.2473558754366536 1108 1 0.15060751479088452 1108 2 -0.010064523860741303 1108 3 0.033516888503889675 1108 4 0.08375057050959356 1108 5 0.0914821685351086 1108 6 0.04844712605954982 1108 7 0.008855408384846442 1108 8 0.008501617874919987 1108 9 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-0.654924996824704 2316 86-87 -0.6936571716061977 2316 88-89 -0.7373456124733551 2316 90-91 -0.6645895141696414 2316 92-93 -0.536554736555118 2316 94-95 -0.4457597946286356 2316 96-97 -0.639944265463825 2316 98-99 -0.6913178371512032 2316 100-101 -0.5898384125571141 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 0.0 11 0.0 12 0.0 13 8.0 14 40.0 15 121.0 16 389.0 17 883.0 18 1622.0 19 2340.0 20 3015.0 21 4055.0 22 5053.0 23 4587.0 24 3621.0 25 3797.0 26 4956.0 27 6435.0 28 8272.0 29 9667.0 30 11519.0 31 14366.0 32 18207.0 33 23613.0 34 33151.0 35 52205.0 36 93918.0 37 228294.0 38 480947.0 39 150565.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.57287801111135 24.009095004182843 12.504000514811558 23.91402646989425 2 12.820851940913775 23.42605633802817 43.34884919271728 20.404242528340777 3 13.49367099466828 36.484152822637725 30.05288826549652 19.96928791719748 4 9.260465056427018 19.25750537246979 46.23884220595111 25.24318736515208 5 9.665984652537393 41.971921606979706 38.08675734452008 10.27533639596282 6 23.901582777758236 45.66402627296117 19.969818352466394 10.464572596814198 7 24.74573158592093 33.14156649252041 28.811280146639845 13.30142177491882 8 23.71230156176946 37.26688199439805 23.564574727730523 15.456241716101967 9 27.06420798428365 15.574285922627856 23.593485263776472 33.76802082931202 10-11 20.989907048766156 29.549913568649654 32.27925690485689 17.180922477727307 12-13 22.90001844440727 26.965637640316903 31.262283760771748 18.87206015450408 14-15 21.709496296105655 28.198967971072307 27.056058129908678 23.03547760291336 16-17 19.425263690683 31.015686324973295 28.961236718954414 20.597813265389284 18-19 19.666113334448575 30.271089892893755 30.085444789026006 19.97735198363166 20-21 19.267242303749363 30.035988041161055 30.697884898319845 19.99888475676974 22-23 20.385702581788077 29.700642980616216 29.30130011624266 20.612354321353045 24-25 20.16694333277571 30.816615408372044 29.68794636537942 19.328494893472826 26-27 19.05573213573368 31.218446123028485 30.36288298953816 19.362938751699673 28-29 19.797434646893983 27.51078047970722 32.198342810890246 20.493442062508553 30-31 20.051000930799155 29.868401298829422 30.336245833923126 19.744351936448293 32-33 19.788103786250765 29.58667370127781 31.210339162623914 19.41488334984751 34-35 20.19135000193023 29.9477551440594 30.874093328700784 18.986801525309588 36-37 20.4998181294095 28.812548041288053 29.826991311389904 20.860642517912538 38-39 20.381112926955858 30.68844822483304 30.141249844509353 18.78918900370175 40-41 19.452201104090797 29.337802885048447 32.116345605298264 19.09365040556249 42-43 19.95187296521728 28.67178820673178 30.49920002745214 20.8771388005988 44-45 18.893807397494133 29.859693822839322 30.58734713661301 20.659151643053537 46-47 20.808208190174707 28.72609197325132 29.89907048766155 20.566629348912425 48-49 19.517700196883325 30.202373752321638 29.384085479104204 20.895840571690837 50-51 20.040577695993274 28.929580968803215 29.29842622022622 21.73141511497729 52-53 20.150000214469852 28.984356568997093 30.10804991142395 20.7575933051091 54-55 20.461153075712147 29.466570584172985 29.6469397296822 20.42533661043267 56-57 20.21635718667027 29.764040268859404 29.261923451420436 20.75767909304989 58-59 18.977622215645145 28.713095100221764 31.058580295367882 21.250702388765212 60-61 20.082322143292515 31.36091045064854 28.73841486980173 19.818352536257215 62-63 19.62308825705664 29.944988129017315 30.961753345215037 19.47017026871101 64-65 19.932896442447674 29.295781535111836 30.2724805925565 20.49884142988399 66-67 19.828810164155225 30.45450451030099 28.810893352721408 20.905791972822378 68-69 19.796897050181656 29.972333389095496 30.02912500589792 20.20164455482493 70-71 20.67566582165545 28.602085504840584 30.438676635225388 20.28357203827858 72-73 19.543608155001653 30.7742361656222 28.56506800839006 21.11708767098609 74-75 20.209365469495953 30.22364916163735 28.593034877087327 20.97395049177937 76-77 19.362810069788488 30.301844869666674 30.747427434125584 19.587917626419255 78-79 19.684043014073513 29.089017856759874 29.87080336117152 21.356135767995095 80-81 19.384685994689725 31.578412322579812 28.06698322416818 20.96991845856228 82-83 21.17289272646944 29.778795794675144 28.488116225502182 20.560195253353236 84-85 19.087945507500013 30.382185276215722 28.439989190719462 22.089880025564806 86-87 19.27281851990066 30.213869336387383 30.533172052004648 19.98014009170731 88-89 19.87873874569452 29.502472837393245 30.25062946901554 20.368158947896696 90-91 19.72269048139903 30.01115243230259 30.092479400170717 20.17367768612766 92-93 20.649672075596335 30.360266457344093 29.099140833772996 19.89092063328658 94-95 19.72269048139903 28.651370676823955 29.85411760668803 21.771821235088986 96-97 20.52176225587969 29.27968155516379 29.961138062822506 20.23741812613401 98-99 19.43602886770399 28.957266790344548 30.40588506535983 21.200819276591638 100-101 19.85017794800238 28.847651030605682 30.61293296140907 20.689238059982866 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76.0 1 60.0 2 132.0 3 185.0 4 122.0 5 141.0 6 248.5 7 469.5 8 680.5 9 679.0 10 510.0 11 367.0 12 350.0 13 383.5 14 385.0 15 365.0 16 366.5 17 337.5 18 329.5 19 356.0 20 407.0 21 455.0 22 502.5 23 665.0 24 718.0 25 792.5 26 1185.5 27 1642.0 28 1861.0 29 2584.0 30 5059.5 31 8072.5 32 15011.0 33 26608.0 34 38840.5 35 52481.5 36 76329.0 37 97961.5 38 103275.0 39 107225.0 40 96286.5 41 77955.5 42 65920.0 43 59650.5 44 58178.0 45 56337.0 46 47440.0 47 31936.0 48 24298.5 49 18740.5 50 14765.5 51 9308.5 52 4612.5 53 3701.0 54 3305.5 55 3369.5 56 3534.5 57 3631.5 58 4141.0 59 4298.0 60 5166.5 61 5946.0 62 4434.0 63 2474.5 64 1388.5 65 1113.5 66 954.5 67 734.5 68 458.0 69 325.0 70 302.5 71 294.0 72 206.0 73 233.5 74 273.0 75 360.5 76 336.5 77 185.5 78 243.0 79 286.0 80 176.0 81 92.0 82 48.5 83 25.0 84 10.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016299708749941022 2 0.10852174509829154 3 0.0 4 0.0 5 0.0 6 0.004804124684193143 7 0.03096944662488794 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0014155010230211939 30-31 0.0 32-33 0.0 34-35 0.0 36-37 8.578794078916326E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 4.289397039458163E-5 62-63 6.434095559187244E-4 64-65 3.4315176315665303E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.1446985197290817E-4 100-101 0.0013297130822320306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1165665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.487685560553375 #Duplication Level Percentage of deduplicated Percentage of total 1 71.44103521591349 7.49251113464921 2 10.264223299304751 2.152958929728279 3 4.01959459966236 1.2646873272647179 4 2.151849654534845 0.9027169020138744 5 1.4371775779939977 0.7536333266339361 6 1.0314328899706666 0.6490406296095119 7 0.7644271170527542 0.5611949866326731 8 0.6231785808995125 0.5228560803636768 9 0.4983743190220458 0.47041138344223177 >10 4.901625511031205 11.19960080379214 >50 1.1248285485170986 8.415974783416777 >100 1.4228670617373225 31.859325803852755 >500 0.22660779517570742 16.260276028796742 >1k 0.0911357437119693 16.59764866353514 >5k 0.001642085472287735 0.8971632162683466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 5415 0.4645416993733191 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 5004 0.42928285570897295 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 4157 0.35662046986055174 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 4118 0.35327474016977434 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 3825 0.3281388735185495 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 3528 0.302659855104168 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3339 0.28644593429501614 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 3266 0.28018341461740726 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 3207 0.2751219261108466 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 3197 0.27426404670295496 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3194 0.2740066828805875 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 3148 0.270060437604286 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 3085 0.2646557973345687 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 3034 0.2602806123543214 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 3013 0.2584790655977489 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 2799 0.240120446268868 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2735 0.23463001805836153 No Hit CAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGC 2689 0.23068377278206 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2664 0.22853907426233097 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 2591 0.222276554584722 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2508 0.21515615549922146 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 2493 0.21386933638738403 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 2492 0.21378354844659486 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 2462 0.21120991022291996 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2433 0.2087220599400342 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 2405 0.20631999759793768 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 2315 0.19859908292691295 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 2306 0.1978269914598105 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2252 0.19319444265719568 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 2243 0.1924223511900932 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 2224 0.1907923803150991 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 2211 0.18967713708483996 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 2109 0.18092676712434533 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 2038 0.17483582332831474 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 2011 0.17251954892700733 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 1959 0.16805857600597085 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 1954 0.16762963630202501 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1940 0.16642860513097674 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 1886 0.16179605632836191 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 1879 0.16119554074283776 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 1863 0.15982293369021117 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1682 0.14429531640737261 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 1677 0.1438663767034268 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 1639 0.14060643495343858 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 1631 0.1399201314271253 No Hit CTATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAG 1624 0.13931961584160113 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1606 0.1377754329073962 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 1594 0.13674597761792626 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 1558 0.13365761174951638 No Hit TGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAA 1516 0.1300545182363715 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 1510 0.12953979059163653 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1510 0.12953979059163653 No Hit CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT 1496 0.12833875942058823 No Hit CTTCTACTGTTCGCAGTCATAGCCACAGCATTTATAGGCTACGTCCTTCC 1484 0.1273093041311183 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1472 0.12627984884164833 No Hit CCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGA 1400 0.12010311710482857 No Hit ATGTTATGTTTACTCCTACGAATATGATGGCGAAGTGGGCTTTTGCTCAT 1368 0.11735790299957534 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1366 0.11718632711799702 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 1365 0.11710053917720786 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 1364 0.11701475123641869 No Hit GTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGA 1330 0.11409796124958714 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1318 0.11306850596011718 No Hit GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC 1311 0.11246799037459304 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 1308 0.11221062655222555 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 1301 0.11161011096670141 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 1299 0.11143853508512308 No Hit ATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAG 1279 0.10972277626933981 No Hit TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC 1278 0.10963698832855066 No Hit CATTTATAGAAACCTGAAACATTGGAGTACTTCTACTGTTCGCAGTCATA 1277 0.10955120038776149 No Hit GAATTAAGATGATTGAGAAGTATGAGATGGAGGCTAGTTGGCCAATGATA 1265 0.10852174509829154 No Hit ATATGGTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGAT 1252 0.10740650186803241 No Hit ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA 1233 0.1057765309930383 No Hit CACTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCG 1219 0.10457549982199002 No Hit CTTCTGAGCGTTCTATCATTCTAGCCTCGTACCAACACATGATCTAGGAG 1214 0.10414656011804421 No Hit GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT 1206 0.1034602565917309 No Hit ATAGAAGAGACAGTGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTA 1204 0.10328868071015258 No Hit ATATATCAATGATGACGAGACGTAATTCGTGAAGGAACCTACCAAGGCCA 1185 0.10165870983515847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.10122977013121265 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 1178 0.10105819424963433 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 1175 0.10080083042726684 No Hit CTATCATTCTAGCCTCGTACCAACACATGATCTAGGAGGCTGCTGACCTC 1174 0.10071504248647767 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1171 0.10045767866411019 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.578794078916327E-5 0.0 3 0.0 0.0 0.0 8.578794078916327E-5 0.0 4 0.0 0.0 0.0 8.578794078916327E-5 0.0 5 0.0 0.0 0.0 8.578794078916327E-5 0.0 6 0.0 0.0 0.0 8.578794078916327E-5 0.0 7 0.0 0.0 0.0 8.578794078916327E-5 0.0 8 0.0 0.0 0.0 8.578794078916327E-5 0.0 9 0.0 0.0 0.0 8.578794078916327E-5 0.0 10-11 0.0 0.0 0.0 1.286819111837449E-4 0.0 12-13 0.0 0.0 0.0 1.7157588157832654E-4 0.0 14-15 0.0 0.0 0.0 2.1446985197290817E-4 0.0 16-17 0.0 0.0 0.0 5.147276447349796E-4 0.0 18-19 0.0 0.0 0.0 9.43667348680796E-4 0.0 20-21 0.0 0.0 0.0 0.0021018045493345003 0.0 22-23 0.0 0.0 0.0 0.0056191101216901945 0.0 24-25 0.0 0.0 0.0 0.010165870983515847 0.0 26-27 0.0 0.0 0.0 0.011795841858509949 0.0 28-29 0.0 0.0 0.0 0.01286819111837449 0.0 30-31 0.0 0.0 0.0 0.015484723312443969 0.0 32-33 0.0 0.0 0.0 0.019645438440718387 0.0 34-35 0.0 0.0 0.0 0.024749820917673603 0.0 36-37 0.0 0.0 0.0 0.03204179588475248 0.0 38-39 0.0 0.0 0.0 0.047354943315618125 0.0 40-41 0.0 0.0 0.0 0.06691459381554735 0.0 42-43 0.0 0.0 0.0 0.08621688049310908 0.0 44-45 0.0 0.0 0.0 0.10710624407527033 0.0 46-47 0.0 0.0 0.0 0.13147001925939272 0.0 48-49 0.0 0.0 0.0 0.157892705022455 0.0 50-51 0.0 0.0 0.0 0.18877636370655376 0.0 52-53 0.0 0.0 0.0 0.2235633736965595 0.0 54-55 0.0 0.0 0.0 0.25835038368656515 0.0 56-57 0.0 0.0 0.0 0.2949389404331433 0.0 58-59 0.0 0.0 0.0 0.3374039711237791 0.0 60-61 0.0 0.0 0.0 0.3876328104558342 0.0 62-63 0.0 0.0 0.0 0.4393200447813051 0.0 64-65 0.0 0.0 0.0 0.4951251002646558 0.0 66-67 0.0 0.0 0.0 0.5599807835012632 0.0 68-69 0.0 0.0 0.0 0.6354312774253323 0.0 70-71 0.0 0.0 0.0 0.7243933720236946 0.0 72-73 0.0 0.0 0.0 0.8216768968786058 0.0 74-75 0.0 0.0 0.0 0.9146710246940588 0.0 76-77 0.0 0.0 0.0 1.0157721129140875 0.0 78-79 0.0 0.0 0.0 1.1395212175024558 0.0 80-81 0.0 0.0 0.0 1.2825297147979908 0.0 82-83 0.0 0.0 0.0 1.4349319916099395 0.0 84-85 0.0 0.0 0.0 1.5979719730797441 0.0 86-87 0.0 0.0 0.0 1.7750811768389718 0.0 88-89 0.0 0.0 0.0 1.9671174822955138 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGATG 20 0.0020745187 71.24624 9 GACATAT 420 0.0 70.23585 1 TACCTGG 85 0.0 67.14457 2 ACAACGC 25 0.0050180824 57.009216 3 TAAGATC 25 0.0050180824 57.009216 3 GCATGAC 25 0.0050223446 56.996986 9 CCGCACA 25 0.0050223446 56.996986 9 ACATATA 540 0.0 54.60677 2 AGATCCT 35 2.7857348E-4 54.29449 5 TAACCGC 40 5.383918E-4 47.507683 6 GGTATCA 1370 0.0 45.14808 1 CATATAG 660 0.0 44.628426 3 ATGGGTA 625 0.0 43.327003 5 CGGGAGT 340 0.0 43.315826 4 AACGCGT 55 4.958622E-5 43.1888 6 CGCGTAC 55 4.9649032E-5 43.17954 8 GTCGGGA 350 0.0 42.12522 2 TCGGGAG 350 0.0 42.078228 3 GGATGCC 170 0.0 41.90955 8 CATGGGT 795 0.0 40.6355 4 >>END_MODULE