FastQCFastQC Report
Fri 10 Feb 2017
SRR2568673_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568673_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4171780
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50300.039.7584531
GTACATG151550.034.3220751
TCAACGC58100.033.601624
TACATGG156400.032.6806642
CAACGCA59800.032.566965
ACATGGG162000.030.610813
AACGCAG63950.030.3796316
ATCAACG65200.029.8693373
TATCAAC65900.029.8421632
GGTATCA32850.029.2099861
CATGGGG123300.025.579944
ACGCAGA79000.024.4117377
TATAACG1951.571425E-824.3601952
GAGTACA102400.023.3336891
AGTACAT99050.023.1637042
CGCAGAG88800.021.3966988
GTACCGT7900.019.8420526
CATGGGA57550.019.7263664
GTATTAC7300.019.5215261
TACCGTA4650.019.4088767
Produced by FastQC (version 0.11.5)