FastQCFastQC Report
Fri 10 Feb 2017
SRR2568673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4171780
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43650.035.422291
GTACATG132400.032.8783951
TACATGG134100.031.9233072
ACATGGG139700.029.8282953
TCAACGC52850.027.870324
GGTATCA32700.027.4973981
CAACGCA54200.027.1761325
ATCAACG57500.025.6164593
AACGCAG57800.025.48356
TATCAAC59450.025.2830412
CATGGGG101650.024.259694
GAGTACA91550.022.6571711
AGTACAT87600.021.719512
TATACCG4400.021.5974545
ACGCAGA72550.020.1710327
ACCGTAT6900.019.2742738
CATGGGA52200.018.932944
GTATATA10350.018.386421
CGCAGAG79900.018.071578
TACCGTA6550.017.409447