FastQCFastQC Report
Fri 10 Feb 2017
SRR2568672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872611
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48350.034.384831
TCAACGC51700.030.6855834
GGTATCA28800.030.3473421
CAACGCA52450.030.3373625
ATCAACG52650.030.041693
GTACATG112900.029.1143741
AACGCAG57400.027.8042686
TACATGG119600.026.8082162
ACATGGG113500.026.783213
TATCAAC60550.026.5938282
CATGGGG70700.024.2530464
ACGCAGA66150.023.9110417
CGCAGAG68700.022.7469568
GTATATA31600.020.8940721
CTATACG2001.2864046E-518.999344
GTATAGG17500.018.4572161
AGTACAT77500.016.6710342
GCAGAGT89800.016.6616639
GAGTACA84500.016.6391181
AGAGTAC72400.016.49977110-11
Produced by FastQC (version 0.11.5)