FastQCFastQC Report
Fri 10 Feb 2017
SRR2568672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2568672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872611
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49000.034.1729431
TCAACGC46800.033.6026544
ATCAACG48300.032.4607283
CAACGCA48500.032.4248285
GGTATCA28350.031.7135281
AACGCAG51950.030.5458586
GTACATG100700.029.288561
TATCAAC55800.028.724162
ACATGGG99050.027.9644013
TACATGG107100.026.6892642
ACGCAGA61600.025.6050937
CGCAGAG64150.024.581888
CATGGGG62900.023.4909824
TAATACT30650.018.7562644
GTATATA34750.018.7543321
GCAGAGT88850.017.1601569
GTATAGC17200.017.147431
GTACCGT4455.456968E-1217.0824996
GTATAGA17400.016.9503351
GTGTAGA25900.016.3465481
Produced by FastQC (version 0.11.5)