##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2568671_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 128094 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.367886083657314 30.0 15.0 33.0 7.0 34.0 2 24.48806345340141 30.0 15.0 33.0 7.0 34.0 3 24.4226895873343 30.0 15.0 34.0 7.0 34.0 4 26.851366964885163 35.0 17.0 37.0 7.0 37.0 5 26.78767155370275 35.0 17.0 37.0 7.0 37.0 6 27.08269708183053 35.0 17.0 37.0 8.0 37.0 7 27.101948569019626 35.0 17.0 37.0 8.0 37.0 8 27.095617281059223 35.0 17.0 37.0 8.0 37.0 9 28.237317907161927 35.0 17.0 39.0 8.0 39.0 10-11 28.08518353708995 35.0 17.0 39.0 8.0 39.0 12-13 28.01983309132356 35.0 16.0 39.0 8.0 39.0 14-15 28.63819929114557 36.0 17.0 40.5 8.0 41.0 16-17 28.617573812981092 35.5 17.0 40.0 8.0 41.0 18-19 28.703592674129936 36.0 16.5 41.0 8.0 41.0 20-21 28.68995034896248 36.0 16.0 41.0 8.0 41.0 22-23 28.574507783346604 35.5 15.5 41.0 8.0 41.0 24-25 28.37905756709916 35.0 15.0 41.0 8.0 41.0 26-27 28.162849157649852 35.0 13.0 40.5 8.0 41.0 28-29 27.95843286961138 35.0 13.0 40.0 8.0 41.0 30-31 27.73644745265196 34.0 13.0 40.0 8.0 41.0 32-33 27.391505456930066 34.0 13.0 40.0 7.0 41.0 34-35 27.219549705684887 33.5 13.0 40.0 7.0 41.0 36-37 27.042558589785628 33.0 12.0 40.0 7.0 41.0 38-39 26.793795962340155 33.0 12.0 40.0 7.0 41.0 40-41 26.5211524349306 33.0 11.0 40.0 7.0 41.0 42-43 26.206668540290725 31.5 10.0 40.0 7.0 41.0 44-45 25.83663169235093 30.0 9.0 40.0 7.0 41.0 46-47 25.594141802114073 30.0 9.0 40.0 7.0 41.0 48-49 25.44210892001187 29.0 8.0 40.0 7.0 41.0 50-51 24.68230362077849 28.0 8.5 38.5 7.0 40.0 52-53 24.82731431604915 28.0 8.0 38.5 7.0 40.0 54-55 25.072001811169923 28.0 8.0 39.5 7.0 41.0 56-57 24.813640763814075 28.0 8.0 40.0 7.0 41.0 58-59 24.52011803831561 27.0 8.0 39.0 7.0 41.0 60-61 24.16426998922666 26.0 8.0 39.0 7.0 41.0 62-63 23.850840008119036 26.0 8.0 38.0 7.0 41.0 64-65 23.59504348369166 26.0 8.0 37.5 7.0 40.5 66-67 23.26915780598623 25.0 8.0 37.0 7.0 40.0 68-69 22.818086717566786 24.0 8.0 36.0 7.0 39.0 70-71 22.2951972769997 23.5 8.0 35.0 7.0 38.0 72-73 21.80378081721236 22.5 7.5 35.0 7.0 37.0 74-75 21.450356769247584 22.0 7.0 35.0 7.0 37.0 76-77 20.995218355270346 20.5 7.0 35.0 7.0 36.0 78-79 20.58505472543601 20.0 7.0 34.0 7.0 35.5 80-81 20.183829843708526 20.0 7.0 34.0 7.0 35.0 82-83 19.738859743625774 18.5 7.0 33.5 7.0 35.0 84-85 19.401302949396538 18.0 7.0 33.0 7.0 35.0 86-87 19.243992692866176 17.0 7.0 33.0 7.0 35.0 88-89 18.94439630271519 16.5 7.0 33.0 7.0 35.0 90-91 18.68889643543023 15.0 7.0 33.0 7.0 35.0 92-93 18.390443736630914 15.0 7.0 32.0 7.0 35.0 94-95 18.115173232157634 13.0 7.0 32.0 7.0 35.0 96-97 17.882562805439754 11.5 7.0 31.0 6.0 35.0 98-99 17.638152450544133 10.5 7.0 31.0 5.0 35.0 100-101 16.712016175621027 10.0 7.0 28.5 5.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1851275557684424 1101 2 0.6848376689185116 1101 3 0.7357294852884451 1101 4 1.6458695906908325 1101 5 1.435443494746231 1101 6 0.10172198983491754 1101 7 1.0050662877917702 1101 8 0.721808878028682 1101 9 1.264849169152022 1101 10-11 0.7811150374960789 1101 12-13 1.1677509509148223 1101 14-15 1.369429196848401 1101 16-17 0.7411084945994659 1101 18-19 1.2432746721733388 1101 20-21 0.9919137422058881 1101 22-23 1.5583442356610107 1101 24-25 1.1681917998752667 1101 26-27 1.3151812907622933 1101 28-29 1.4848290059471339 1101 30-31 1.4990749709592244 1101 32-33 1.1656479339471666 1101 34-35 1.4207403724398304 1101 36-37 1.547165071717739 1101 38-39 2.049296374323216 1101 40-41 2.5233364317901135 1101 42-43 1.7775122019109908 1101 44-45 1.552982342729031 1101 46-47 1.6218605916050386 1101 48-49 2.1649727915549413 1101 50-51 1.9915007846204276 1101 52-53 2.019170633908228 1101 54-55 2.305282865638734 1101 56-57 2.6735762726011423 1101 58-59 2.489310744508323 1101 60-61 2.154052103546256 1101 62-63 1.892530698182437 1101 64-65 2.246609133050189 1101 66-67 1.9045223483508877 1101 68-69 1.8309326100627636 1101 70-71 2.006811280749062 1101 72-73 0.9010147214891902 1101 74-75 0.991612123664833 1101 76-77 0.9407412261774617 1101 78-79 1.1752123034965969 1101 80-81 1.4648702617561078 1101 82-83 1.8284443639967058 1101 84-85 1.6749738147533044 1101 86-87 1.3578595247636116 1101 88-89 0.5807477479813095 1101 90-91 0.41182724223446243 1101 92-93 1.1196845291390218 1101 94-95 2.0921316042861555 1101 96-97 1.9572927004898197 1101 98-99 1.5354281198905078 1101 100-101 1.2057809391051428 1102 1 0.05051217115305917 1102 2 0.2859772700581118 1102 3 -0.4166921671332098 1102 4 -0.14650932668808636 1102 5 0.27981671411945186 1102 6 0.1270068901198158 1102 7 0.7995819573074385 1102 8 0.6103416415614475 1102 9 0.23315401245686473 1102 10-11 0.8429027867838315 1102 12-13 0.44392616459003165 1102 14-15 0.23748475240395805 1102 16-17 -0.05233879884782411 1102 18-19 -0.6514902850916222 1102 20-21 -0.26414038884824365 1102 22-23 0.06368611600289142 1102 24-25 0.16017897936244907 1102 26-27 0.3064562195372247 1102 28-29 0.061574020192150414 1102 30-31 0.42731571169996485 1102 32-33 0.4006906689899026 1102 34-35 -0.6549362657368114 1102 36-37 -0.16651014195747038 1102 38-39 -0.2210027709759288 1102 40-41 -0.3461436251899457 1102 42-43 -0.23815731375852423 1102 44-45 -0.22817862593193894 1102 46-47 0.09550731525176204 1102 48-49 -0.07167250759035682 1102 50-51 -0.6402655971459552 1102 52-53 -0.2206797934421978 1102 54-55 0.8763298741857461 1102 56-57 0.4337258452507129 1102 58-59 1.1079004880980676 1102 60-61 0.7231404226345717 1102 62-63 0.9682073363590717 1102 64-65 1.5612458851869384 1102 66-67 0.8375707813993216 1102 68-69 1.281965373595522 1102 70-71 1.5796389160766964 1102 72-73 1.1825318155062874 1102 74-75 0.5647958843485945 1102 76-77 0.40085518629142314 1102 78-79 0.6730755513598474 1102 80-81 0.06511954950539334 1102 82-83 0.5474614580138031 1102 84-85 1.014539341818832 1102 86-87 0.4889136558177434 1102 88-89 1.0080981753317353 1102 90-91 0.5703030257102455 1102 92-93 0.6394494864039793 1102 94-95 0.06951764417219408 1102 96-97 0.628767059464181 1102 98-99 0.5774509119132993 1102 100-101 0.7389005972247986 1103 1 -0.8057815351406497 1103 2 -1.1720646879838448 1103 3 -1.2649439153849578 1103 4 -0.8704603756391336 1103 5 -0.9318965725938355 1103 6 -1.0284126902997635 1103 7 -1.5341593014338208 1103 8 -0.1300429738231692 1103 9 -1.12471312041027 1103 10-11 -0.39354826216722216 1103 12-13 -1.0082591500952809 1103 14-15 -0.9863788839596808 1103 16-17 -0.5383527848618144 1103 18-19 -0.28829098439232226 1103 20-21 -0.3120424867503395 1103 22-23 -1.2441810168642427 1103 24-25 -0.7780203213368537 1103 26-27 -0.8534214028403966 1103 28-29 -0.5150868189686939 1103 30-31 -1.6692751973909452 1103 32-33 -1.4272813589821283 1103 34-35 -1.0775761258766714 1103 36-37 -1.590461190908524 1103 38-39 -1.1570167569899148 1103 40-41 -1.2815457230920408 1103 42-43 -1.3991712997725116 1103 44-45 -1.2300142902676043 1103 46-47 -1.9588633141188687 1103 48-49 -1.309522157940009 1103 50-51 -1.0303005621809191 1103 52-53 -1.660627345889747 1103 54-55 -1.17375753840167 1103 56-57 -1.1584944344695671 1103 58-59 -0.7212953160977342 1103 60-61 -1.4217896472954976 1103 62-63 -1.9546073489556122 1103 64-65 -1.4112191497780948 1103 66-67 -2.1638278199992804 1103 68-69 -1.9686465145163616 1103 70-71 -2.127616328678549 1103 72-73 -1.2547933593188887 1103 74-75 -1.2134383813856715 1103 76-77 -1.5040399186036808 1103 78-79 -1.5805957773114834 1103 80-81 -1.5029748560890077 1103 82-83 -1.9773637168113716 1103 84-85 -0.7264571616776685 1103 86-87 -1.2700024280983406 1103 88-89 -0.8599088176752581 1103 90-91 -0.8130885826813596 1103 92-93 -0.4123861779316833 1103 94-95 -0.9449054327508826 1103 96-97 -2.0851315419344196 1103 98-99 -1.359437199974817 1103 100-101 -1.8166938083696067 1104 1 -0.7061767920576436 1104 2 -0.5926180476676386 1104 3 -0.4404379865312009 1104 4 -0.3001046444138389 1104 5 0.13608761712948336 1104 6 -0.1339462871377073 1104 7 -0.3175585109199872 1104 8 0.03517441748117989 1104 9 0.04761889540000652 1104 10-11 -0.19710557442018484 1104 12-13 -0.5519350394233413 1104 14-15 -0.18697176933517312 1104 16-17 -0.342305354031776 1104 18-19 -0.7950103519812508 1104 20-21 -0.1126353721258333 1104 22-23 0.4324988250329902 1104 24-25 0.839568611469474 1104 26-27 -0.042749466081506426 1104 28-29 0.5633716790550274 1104 30-31 0.20503310300431465 1104 32-33 0.09801508370561862 1104 34-35 0.8198547041628572 1104 36-37 0.06902497509938499 1104 38-39 -0.050099760942483584 1104 40-41 0.10481791327159584 1104 42-43 0.2968761310575303 1104 44-45 -0.0966150807814401 1104 46-47 -0.17230204929673576 1104 48-49 -0.3800755176238013 1104 50-51 -0.6119211155406035 1104 52-53 0.24767304936716883 1104 54-55 -0.4767219652791397 1104 56-57 0.0379482532774702 1104 58-59 0.01902088943586122 1104 60-61 0.7284079811630022 1104 62-63 0.84124245341593 1104 64-65 0.28996662097289416 1104 66-67 0.5278717847438017 1104 68-69 0.6953851060369978 1104 70-71 -0.2997507160303243 1104 72-73 -0.28799494034655737 1104 74-75 0.30296478066966515 1104 76-77 -0.17439564982897693 1104 78-79 0.355767859052154 1104 80-81 0.09860617157897522 1104 82-83 0.8459564412913956 1104 84-85 -0.138512497646051 1104 86-87 -0.15280875220901535 1104 88-89 0.05985402817454144 1104 90-91 -0.2520213890054741 1104 92-93 0.7101434663370902 1104 94-95 0.28770326290129233 1104 96-97 0.37178545411300945 1104 98-99 -0.012599255310782098 1104 100-101 -0.06293886765814705 1105 1 0.24762755576844242 1105 2 0.9927080392888818 1105 3 1.39512101967998 1105 4 0.5959357282569684 1105 5 1.1603112196139556 1105 6 0.2836002967132245 1105 7 0.24944459467007718 1105 8 0.17352845474825784 1105 9 0.9549946717975217 1105 10-11 0.6880264131574556 1105 12-13 0.5095168239306922 1105 14-15 0.9964133238325275 1105 16-17 1.2055595528005263 1105 18-19 0.8419849896336551 1105 20-21 0.49307114961329646 1105 22-23 0.8569553467721214 1105 24-25 0.8542037046371718 1105 26-27 1.1089974283284327 1105 28-29 1.1323157784339095 1105 30-31 0.43177999741424955 1105 32-33 0.5188225371217712 1105 34-35 0.5088686793181374 1105 36-37 0.6562920558447267 1105 38-39 0.9743955806724252 1105 40-41 0.7860679132715944 1105 42-43 0.7831338950326838 1105 44-45 1.0149532421999297 1105 46-47 0.9097243482187913 1105 48-49 1.2150719979041469 1105 50-51 1.5513552819749208 1105 52-53 1.2999246021621964 1105 54-55 1.7482392148450856 1105 56-57 1.8516516694265377 1105 58-59 1.8479416968892757 1105 60-61 1.3890058072499585 1105 62-63 2.0856523913041265 1105 64-65 1.6106964346374895 1105 66-67 1.7657988033773435 1105 68-69 2.4031879010680512 1105 70-71 1.7561499050876854 1105 72-73 1.5101417056161779 1105 74-75 1.1200843458870544 1105 76-77 1.8215282632144998 1105 78-79 1.5611250019092964 1105 80-81 2.2155316374174845 1105 82-83 1.7991784909808324 1105 84-85 2.1007343967638867 1105 86-87 2.660273545927634 1105 88-89 1.8089223511559105 1105 90-91 1.6307425861498075 1105 92-93 1.3290099259644172 1105 94-95 1.8452731386776868 1105 96-97 1.4364593671564876 1105 98-99 1.8044426701550549 1105 100-101 1.487526970851171 1106 1 -0.00653911089822401 1106 2 -0.06145862737778529 1106 3 -0.22630755174859374 1106 4 -0.04394522412398416 1106 5 -0.009926875624138631 1106 6 0.11098124909417706 1106 7 0.7309922137176947 1106 8 0.053290359510164365 1106 9 0.7389232432260933 1106 10-11 0.14963355601459938 1106 12-13 0.7955287286925952 1106 14-15 0.7791514190706224 1106 16-17 0.44157145756242855 1106 18-19 0.6279968943955581 1106 20-21 -0.08401218372003783 1106 22-23 -0.49304465322788005 1106 24-25 0.03069179987527093 1106 26-27 0.2836998092808152 1106 28-29 0.39779196891009505 1106 30-31 0.1481490450332963 1106 32-33 0.17120348950272302 1106 34-35 -0.8450003683009122 1106 36-37 0.1277206272732947 1106 38-39 -0.03125918123233973 1106 40-41 -0.532682086728407 1106 42-43 -0.3150803906816009 1106 44-45 -0.17433247208578706 1106 46-47 0.2945457767902191 1106 48-49 -0.3405827640006116 1106 50-51 -1.1075732894536472 1106 52-53 -0.3280515883139934 1106 54-55 -0.8586060232501573 1106 56-57 -0.8194792829544149 1106 58-59 -0.7148559221583426 1106 60-61 -0.6922441927500422 1106 62-63 -1.2667285610768282 1106 64-65 -1.0006130891720382 1106 66-67 -1.158903577575039 1106 68-69 -0.8167525751224254 1106 70-71 -1.624207237769454 1106 72-73 -1.526763056288587 1106 74-75 -0.944498987446277 1106 76-77 -0.6597217367854995 1106 78-79 -0.5567321409478474 1106 80-81 -0.5957778863920389 1106 82-83 0.4506665862189294 1106 84-85 -0.5370632222837308 1106 86-87 -0.657881215977131 1106 88-89 -0.7261966964631377 1106 90-91 -0.07937646146924138 1106 92-93 0.10996230691679898 1106 94-95 -0.4574054327508854 1106 96-97 -0.1718739661768467 1106 98-99 -0.12799780603542033 1106 100-101 0.1488364946606957 1107 1 0.4750435221549978 1107 2 0.2947038376082105 1107 3 0.026423540688380598 1107 4 0.19730127447545698 1107 5 0.37459693389967086 1107 6 0.8434742462930558 1107 7 0.3686672837457081 1107 8 0.5170158497062438 1107 9 1.4103518146546676 1107 10-11 1.2859780938297263 1107 12-13 1.146859260905483 1107 14-15 0.7159511389585802 1107 16-17 1.4463683763299358 1107 18-19 1.3790123005580206 1107 20-21 1.5642371159998518 1107 22-23 1.1883629097973305 1107 24-25 1.5382898390909574 1107 26-27 1.0021171762275962 1107 28-29 1.472651912887688 1107 30-31 2.336454367162151 1107 32-33 1.588150268214207 1107 34-35 2.263332965032422 1107 36-37 1.4020903751724525 1107 38-39 1.7650993621850297 1107 40-41 1.7695237956245364 1107 42-43 1.8855498614192392 1107 44-45 1.4188397968217785 1107 46-47 0.9530541801515646 1107 48-49 0.6727925861394439 1107 50-51 0.6579729290337468 1107 52-53 1.1218783836748045 1107 54-55 0.9034387946770188 1107 56-57 0.9075865433761194 1107 58-59 1.4858093439481017 1107 60-61 1.278974294644918 1107 62-63 1.6087616350016063 1107 64-65 1.143784669931609 1107 66-67 1.7923219126210412 1107 68-69 1.4402152119924203 1107 70-71 0.8948053672725607 1107 72-73 0.9008980081371831 1107 74-75 0.5520696400046994 1107 76-77 0.7214757422060956 1107 78-79 0.40618802711938073 1107 80-81 0.4350694525435337 1107 82-83 0.41446210442621023 1107 84-85 0.8861860774361574 1107 86-87 0.43889749550746515 1107 88-89 0.7921156284668349 1107 90-91 1.0981795609397231 1107 92-93 1.1898292536955104 1107 94-95 0.46475843279533535 1107 96-97 0.7836232327027055 1107 98-99 0.7632136785584187 1107 100-101 0.6356362145486507 1108 1 0.40482452546541126 1108 2 -0.5357010516202081 1108 3 -0.27100452144556186 1108 4 -0.20682401200276956 1108 5 0.4211337304364662 1108 6 -0.03295814484521742 1108 7 -0.1599168771913959 1108 8 -0.16034600412620037 1108 9 -0.49441009010723747 1108 10-11 -0.5829422015611598 1108 12-13 -0.5582591500952816 1108 14-15 -0.4878940354748309 1108 16-17 -1.1216861181951465 1108 18-19 -0.9473818934832288 1108 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-0.46923186199268585 2316 74-75 0.09485038866780471 2316 76-77 0.2825910974390986 2316 78-79 -0.11482037624196195 2316 80-81 0.32697166797516175 2316 82-83 0.16809082864317304 2316 84-85 0.3556283998196541 2316 86-87 0.22919543286422694 2316 88-89 0.6507863694905147 2316 90-91 0.31284724619564486 2316 92-93 0.3384159611057491 2316 94-95 0.5457362784790654 2316 96-97 0.6132641799907113 2316 98-99 0.3285307144636853 2316 100-101 0.21966982799402857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 63.0 7 2385.0 8 5989.0 9 5139.0 10 5048.0 11 5827.0 12 6163.0 13 5529.0 14 4550.0 15 3460.0 16 2919.0 17 2637.0 18 2480.0 19 2487.0 20 2755.0 21 2921.0 22 2371.0 23 2187.0 24 2130.0 25 1739.0 26 1647.0 27 1768.0 28 1860.0 29 1918.0 30 2045.0 31 2271.0 32 2722.0 33 3414.0 34 4335.0 35 5656.0 36 8134.0 37 12313.0 38 10811.0 39 416.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.06244193399799 22.608071076690063 13.510348435048053 17.8191385542639 2 16.638429768197184 25.900392714099453 35.954810552532344 21.506366965171022 3 23.383974018674078 31.62882927895575 26.874434000562097 18.112762701808073 4 15.78996674317298 19.249925835714397 33.88527175355598 31.074835667556638 5 13.335259077672557 39.640406280008435 29.597389315241745 17.426945327077267 6 27.250796327524828 34.695677971394666 18.330366622946727 19.723159078133783 7 24.772812441446508 34.8088813940416 20.02529510961214 20.393011054899755 8 27.411195253337496 28.981185104223595 21.014911390428605 22.592708252010304 9 29.817384040036536 17.54643473372735 19.079034688444214 33.5571465377919 10-11 24.33373541478801 28.637958831543486 23.91799099805986 23.110314755608645 12-13 30.09403654504503 27.632457246357014 22.965371598543193 19.30813461005477 14-15 27.641809920839382 26.737786313176258 24.70100082751729 20.919402938467062 16-17 21.3351132761878 30.538510781145096 25.799022592783427 22.32735334988368 18-19 22.672891286443107 29.09085941128941 28.05645875864115 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23.611750837282287 26.958928278672527 27.105696642283338 22.323624241761845 46-47 22.724824145711185 28.892801099235687 27.070630577176807 21.31174417787632 48-49 22.07913874066585 29.413349051264138 27.10484302237073 21.402669185699285 50-51 19.654539776719492 29.08306659380123 28.70833008041221 22.554063549067063 52-53 20.175418052367792 29.305431948412885 25.348181803987696 25.170968195231623 54-55 20.878027073867628 27.693724920761316 25.286508345433823 26.141739659937237 56-57 22.81644729651662 25.630786765968743 25.567942292379037 25.984823645135602 58-59 24.6553312411198 25.16160007494496 25.47426108951239 24.708807594422847 60-61 23.19273346136431 25.51641763080238 25.96842943463394 25.32241947319937 62-63 22.196069246833343 25.89573940706911 25.642016511505357 26.26617483459219 64-65 22.26208134905145 27.443203997189475 24.951986884222034 25.342727769537042 66-67 23.229878641439907 26.232419793663126 23.929378608260503 26.608322956636464 68-69 22.577245134971598 27.060683543809656 24.967501415103545 25.3945699061152 70-71 23.741641847556668 27.311299948865475 24.699145546026926 24.24791265755093 72-73 23.61781191937171 27.403313191874716 25.757256389838712 23.221618498914857 74-75 23.31451902509095 28.895576685871315 24.658453947882027 23.131450341155713 76-77 23.897294174590535 27.644932627601605 25.022249285680825 23.43552391212703 78-79 24.70646556435118 27.73471044701547 25.169406841850517 22.38941714678283 80-81 25.90581336789628 27.155076497846974 24.810365760820766 22.128744373435982 82-83 24.505077083439065 26.750237164797326 26.022150996872938 22.72253475489067 84-85 24.194688123431053 27.70483675134593 25.237074602858563 22.86340052236446 86-87 24.988873533063174 27.49877023260172 24.35993534937106 23.152420884964044 88-89 25.142009408733333 26.321419508481526 24.083624509555136 24.452946573230005 90-91 26.367959148617203 24.73101488202133 24.060308883926478 24.84071708543498 92-93 27.72921928615764 24.93997962234983 23.523693898025865 23.80710719346666 94-95 26.747431998219675 24.76057360824259 23.814580706117525 24.67741368742021 96-97 25.90890813908549 24.974722730803915 24.564040303093 24.552328827017593 98-99 26.496894361440894 26.04559100828821 22.12284352344552 25.33467110682538 100-101 26.59165794774896 25.218233208926087 22.39408466980027 25.79602417352468 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 31.0 1 22.5 2 15.5 3 23.5 4 22.5 5 17.0 6 23.5 7 37.5 8 49.5 9 46.5 10 36.0 11 27.5 12 27.0 13 30.5 14 39.5 15 48.5 16 44.0 17 49.0 18 66.5 19 77.0 20 104.0 21 147.5 22 180.0 23 226.0 24 316.0 25 444.0 26 573.5 27 718.5 28 912.5 29 1125.0 30 1354.5 31 1482.0 32 1627.5 33 1745.0 34 1773.5 35 1862.5 36 1984.5 37 2091.5 38 2136.0 39 2138.0 40 2333.5 41 2628.0 42 2901.0 43 3041.0 44 3023.0 45 3553.5 46 4578.0 47 6555.0 48 9812.5 49 10464.0 50 9364.0 51 8305.0 52 5924.5 53 4049.0 54 3232.5 55 2915.5 56 2592.5 57 2265.0 58 2095.5 59 1980.0 60 1732.0 61 1555.0 62 1385.5 63 1134.5 64 971.5 65 823.5 66 718.0 67 662.0 68 586.0 69 500.5 70 454.5 71 368.5 72 291.5 73 269.0 74 231.0 75 185.0 76 162.0 77 144.5 78 117.0 79 100.0 80 86.0 81 67.5 82 57.5 83 48.0 84 39.5 85 32.5 86 25.5 87 17.0 88 14.5 89 13.0 90 10.0 91 8.5 92 5.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005464736833887614 2 0.008587443596109108 3 0.0015613533811107466 4 0.0 5 0.003903383452776867 6 0.0046840601433322405 7 0.0046840601433322405 8 0.0031227067622214932 9 0.008587443596109108 10-11 0.00819710525083142 12-13 0.004293721798054554 14-15 0.0 16-17 0.0 18-19 3.9033834527768665E-4 20-21 3.9033834527768665E-4 22-23 0.0 24-25 0.0019516917263884335 26-27 0.004293721798054554 28-29 0.0019516917263884335 30-31 0.0117101503583306 32-33 3.9033834527768665E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 7.806766905553733E-4 46-47 0.003903383452776867 48-49 0.0019516917263884335 50-51 0.0031227067622214932 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0011710150358330601 64-65 0.0031227067622214932 66-67 0.0019516917263884335 68-69 0.008977781941386794 70-71 3.9033834527768665E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.014442518775274407 82-83 0.014442518775274407 84-85 0.017565225537495902 86-87 0.01639421050166284 88-89 0.016784548846940528 90-91 0.015613533811107468 92-93 0.009758458631942168 94-95 0.02146860899027277 96-97 0.011319812013052913 98-99 0.016003872156385154 100-101 0.015613533811107468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 128094.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.9304417068715 #Duplication Level Percentage of deduplicated Percentage of total 1 93.64930505458567 60.806907427358034 2 2.760544413985476 3.5848673630302743 3 1.0700716587313037 2.084406763782847 4 0.5626893666137642 1.461426764719659 5 0.35107969028038283 1.1397879682108452 6 0.2308469196364161 0.8993395475197902 7 0.1683258789015534 0.765063156744266 8 0.14668398018563938 0.7619404499820445 9 0.10941182128600972 0.6393742095648508 >10 0.7899293031308614 9.961434571486565 >50 0.08897225027653537 3.890111949037425 >100 0.05891405761554369 7.641263447155995 >500 0.012023277064396671 5.139975330616578 >1k 0.0012023277064396672 1.2241010507908257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1568 1.2241010507908257 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 882 0.6885568410698394 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 880 0.6869954876887286 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 712 0.5558418036754258 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 679 0.5300794728870986 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 663 0.5175886458382126 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 597 0.46606398426155793 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 566 0.4418630068543414 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 545 0.42546879635267854 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 537 0.4192233828282355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 523 0.4082939091604603 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 496 0.3872156385154652 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 402 0.3138320296032601 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 373 0.2911924055771543 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 359 0.28026293190937906 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 356 0.2779209018377129 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 331 0.2584039845738286 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 326 0.2545006011210517 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 283 0.2209315034271707 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 280 0.21858947335550455 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 264 0.2060986463066186 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 263 0.20531796961606322 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 257 0.20063390947273096 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 254 0.19829187940106485 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 240 0.1873624057332896 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 216 0.16862616515996065 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 215 0.16784548846940528 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 214 0.1670648117788499 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 211 0.1647227817071838 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT 204 0.15925804487329617 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 201 0.15691601480163006 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT 198 0.15457398472996392 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 192 0.1498899245866317 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 190 0.14832857120552095 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 187 0.1459865411338548 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 187 0.1459865411338548 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 178 0.13896045091885645 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 177 0.13817977422830108 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 173 0.1350570674660796 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 168 0.1311536840133027 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 155 0.12100488703608288 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 150 0.11710150358330601 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 140 0.10929473667775227 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT 137 0.10695270660608616 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA 133 0.10382999984386465 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 131 0.10226864646275392 No Hit GTACATGGGAAGGCTAGGGAAAACTGGCAGCCTCCAGATAGAAGTGGAAA 129 0.10070729308164317 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.806766905553734E-4 0.0 3 0.0 0.0 0.0 0.0015613533811107468 0.0 4 0.0 0.0 0.0 0.0023420300716661202 0.0 5 0.0 0.0 0.0 0.0023420300716661202 0.0 6 0.0 0.0 0.0 0.0031227067622214937 0.0 7 0.0 0.0 0.0 0.003903383452776867 0.0 8 0.0 0.0 0.0 0.0046840601433322405 0.0 9 0.0 0.0 0.0 0.009368120286664481 0.0 10-11 0.0 0.0 0.0 0.0117101503583306 0.0 12-13 0.0 0.0 0.0 0.017174887192218213 0.0 14-15 0.0 0.0 0.0 0.026543007478882698 0.0 16-17 0.0 0.0 0.0 0.03239808265804799 0.0 18-19 0.0 0.0 0.0 0.03864349618249098 0.0 20-21 0.0 0.0 0.0 0.051134323231376955 0.0 22-23 0.0 0.0 0.0 0.09407154121192249 0.0 24-25 0.0 0.0 0.0 0.23771605227411122 0.0 26-27 0.0 0.0 0.0 0.4844098864896092 0.0 28-29 0.0 0.0 0.0 0.7955095476759255 0.0 30-31 0.0 0.0 0.0 1.3423735694099646 0.0 32-33 0.0 0.0 0.0 2.0687932299717393 0.0 34-35 0.0 0.0 0.0 3.397895295642263 0.0 36-37 0.0 0.0 0.0 4.718019579371399 0.0 38-39 0.0 0.0 0.0 6.198963261354942 0.0 40-41 0.0 0.0 0.0 8.377441566349711 0.0 42-43 0.0 0.0 0.0 9.579683669804986 0.0 44-45 0.0 0.0 0.0 10.4314019392009 0.0 46-47 0.0 0.0 0.0 11.32215404312458 0.0 48-49 0.0 0.0 0.0 12.182459756116602 0.0 50-51 0.0 0.0 0.0 13.010367386450575 0.0 52-53 0.0 0.0 0.0 13.9038518587912 0.0 54-55 0.0 0.0 0.0 14.575624151014098 0.0 56-57 0.0 0.0 0.0 15.04481084203788 0.0 58-59 0.0 0.0 0.0 15.39884772120474 0.0 60-61 3.903383452776867E-4 0.0 0.0 15.70760535231939 0.0 62-63 7.806766905553734E-4 0.0 0.0 16.010898246600153 0.0 64-65 7.806766905553734E-4 0.0 0.0 16.292332193545363 0.0 66-67 7.806766905553734E-4 0.0 0.0 16.61162895998251 0.0 68-69 7.806766905553734E-4 0.0 0.0 17.04763689165769 0.0 70-71 7.806766905553734E-4 0.0 0.0 17.483644823332867 0.0 72-73 7.806766905553734E-4 0.0 0.0 17.788108732649462 0.0 74-75 7.806766905553734E-4 0.0 0.0 18.109357190812997 0.0 76-77 7.806766905553734E-4 0.0 0.0 18.376738957328214 0.0 78-79 7.806766905553734E-4 0.0 0.0 18.699158430527582 0.0 80-81 7.806766905553734E-4 0.0 0.0 19.044217527753055 0.0 82-83 7.806766905553734E-4 0.0 0.0 19.368198354333536 0.0 84-85 7.806766905553734E-4 0.0 0.0 19.68476275235374 0.0 86-87 7.806766905553734E-4 3.903383452776867E-4 0.0 19.957999594048122 0.0 88-89 7.806766905553734E-4 7.806766905553734E-4 0.0 20.320233578465814 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGTT 20 0.0020689112 71.2582 4 CATATTG 25 0.0050088307 57.006565 5 AAAAGTA 170 0.0 44.71103 2 AGTACTC 180 0.0 42.22708 5 AAAAAGT 185 0.0 41.08581 1 AAAGTAC 190 0.0 40.004604 3 AAGTACT 195 0.0 38.978848 4 TATACAG 50 0.0016095449 38.004375 5 ATTCTGA 65 1.3207059E-4 36.542667 1 TAATACT 55 0.002569655 34.54943 4 TTTGTAC 75 3.0612314E-4 31.670315 3 TATATGG 60 0.003935743 31.670313 2 GTATATG 80 4.4685972E-4 29.690916 1 GTACCCA 70 0.008359531 27.14598 1 GTAGGTC 70 0.008359531 27.14598 8 TGTCGGT 35 0.008374901 27.14598 88-89 CATGGGG 305 0.0 26.478456 4 TTTTGTA 90 8.89802E-4 26.391928 2 TCGCCGG 165 0.0 23.032953 94-95 AGTGTGC 105 0.0021841906 22.62165 9 >>END_MODULE